Restriction map for
pF1KE2691
(Circular) (Six-base) MAPSORT of: pF1KE2691.seq Check: 7746 from: 1 to: 5558
pF1KE2691 5558 bp
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AatII G_ACGT'C
Cuts at: 587 587
Size: 5558
AccI GT'mk_AC
Cuts at: 1040 1616 2596 1040
Size: 576 980 4002
Fragments arranged by size:
4002 980 576
Acc65I G'GTAC_C
Cuts at: 2574 2574
Size: 5558
AclI AA'CG_TT
Cuts at: 5398 5398
Size: 5558
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2338 3383 3815 4562 4830 1276
Size: 1062 1045 432 747 268 2004
Fragments arranged by size:
2004 1062 1045 747 432 268
AfeI AGC'GCT
Cuts at: 1654 1807 2485 1654
Size: 153 678 4727
Fragments arranged by size:
4727 678 153
AflIII A'CryG_T
Cuts at: 399 960 2910 4014 4991 399
Size: 561 1950 1104 977 966
Fragments arranged by size:
1950 1104 977 966 561
AgeI A'CCGG_T
Cuts at: 1760 3946 1760
Size: 2186 3372
AleI CACnn'nnGTG
Cuts at: 400 2068 400
Size: 1668 3890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2413 2413
Size: 5558
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2522 2554 2522
Size: 32 5526
AlwNI CAG_nnn'CTG
Cuts at: 1686 2043 2106 2863 4430 1686
Size: 357 63 757 1567 2814
Fragments arranged by size:
2814 1567 757 357 63
ApaI G_GGCC'C
Cuts at: 2205 2205
Size: 5558
ApaLI G'TGCA_C
Cuts at: 4328 4328
Size: 5558
ApoI r'AATT_y
Cuts at: 851 2562 4911 5167 851
Size: 1711 2349 256 1242
Fragments arranged by size:
2349 1711 1242 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5558
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5558
AvaI C'yCGr_G
Cuts at: 2198 2287 2578 2198
Size: 89 291 5178
Fragments arranged by size:
5178 291 89
BamHI G'GATC_C
Cuts at: 431 2346 2583 431
Size: 1915 237 3406
Fragments arranged by size:
3406 1915 237
BanI G'GyrC_C
Cuts at: 667 2163 2574 2988 3236 3271 667
Size: 1496 411 414 248 35 2954
Fragments arranged by size:
2954 1496 414 411 248 35
BanII G_rGCy'C
Cuts at: 1148 1820 2205 2572 3602 1148
Size: 672 385 367 1030 3104
Fragments arranged by size:
3104 1030 672 385 367
BbeI G_GCGC'C
Cuts at: 2992 3240 2992
Size: 248 5310
BbsI GAAGACnn'nnnn_
Cuts at: 579 1101 1718 2035 2430 579
Size: 522 617 317 395 3707
Fragments arranged by size:
3707 617 522 395 317
BbvCI CC'TCA_GC
Cuts at: 2363 2363
Size: 5558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 396 430 396
Size: 34 5524
BciVI GTATCCnnnnn_n'
Cuts at: 2471 3456 4217 5558 2471
Size: 985 761 1341 2471
Fragments arranged by size:
2471 1341 985 761
BclI T'GATC_A
Cuts at: 345 542 3078 345
Size: 197 2536 2825
Fragments arranged by size:
2825 2536 197
BglI GCCn_nnn'nGGC
Cuts at: 645 749 645
Size: 104 5454
BglII A'GATC_T
Cuts at: 419 3073 5110 419
Size: 2654 2037 867
Fragments arranged by size:
2654 2037 867
BlpI GC'TnA_GC
Cuts at: 223 2670 223
Size: 2447 3111
Bme1580I G_kGCm'C
Cuts at: 670 697 2205 3183 3276 4332 670
Size: 27 1508 978 93 1056 1896
Fragments arranged by size:
1896 1508 1056 978 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 2447 2770 2851 3186 5330 2447
Size: 323 81 335 2144 2675
Fragments arranged by size:
2675 2144 335 323 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3925 3925
Size: 5558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 2155 202
Size: 1953 3605
Bpu10I CC'TnA_GC
Cuts at: 994 2363 2513 994
Size: 1369 150 4039
Fragments arranged by size:
4039 1369 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 2734 3613 4105 4403 468
Size: 2266 879 492 298 1623
Fragments arranged by size:
2266 1623 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1273 5262 1273
Size: 3989 1569
BsaAI yAC'GTr
Cuts at: 402 963 2911 402
Size: 561 1948 3049
Fragments arranged by size:
3049 1948 561
BsaBI GATnn'nnATC
Cuts at: 6 3072 5109 6
Size: 3066 2037 455
Fragments arranged by size:
3066 2037 455
BsaHI Gr'CG_yC
Cuts at: 584 908 1304 2989 3237 3939 5435 584
Size: 324 396 1685 248 702 1496 707
Fragments arranged by size:
1685 1496 707 702 396 324 248
BsaWI w'CCGG_w
Cuts at: 300 1760 2111 2747 3268 3946 4220 4367 4778
Size: 1460 351 636 521 678 274 147 411
Cuts at: 4778 300
Size: 1080
Fragments arranged by size:
1460 1080 678 636 521 411 351 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 279 309 2401 2431 279
Size: 30 2092 30 3406
Fragments arranged by size:
3406 2092 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 955 2005 2008 2326 2380 955
Size: 1050 3 318 54 4133
Fragments arranged by size:
4133 1050 318 54 3
BseYI C'CCAG_C
Cuts at: 1386 1395 1820 2464 2983 3741 4318 4814 1386
Size: 9 425 644 519 758 577 496 2130
Fragments arranged by size:
2130 758 644 577 519 496 425 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1609 856
Size: 753 4805
BsiEI CG_ry'CG
Cuts at: 104 815 3100 3146 4354 104
Size: 711 2285 46 1208 1308
Fragments arranged by size:
2285 1308 1208 711 46
BsiHKAI G_wGCw'C
Cuts at: 1148 2572 3350 3540 4332 1148
Size: 1424 778 190 792 2374
Fragments arranged by size:
2374 1424 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1311 1639 1914 1923 1311
Size: 328 275 9 4946
Fragments arranged by size:
4946 328 275 9
Bsp1286I G_dGCh'C
Cuts at: 670 697 1148 1820 2205 2572 3183 3276 3350
Size: 27 451 672 385 367 611 93 74
Cuts at: 3350 3540 3602 4332 670
Size: 190 62 730 1896
Fragments arranged by size:
1896 730 672 611 451 385 367 190
93 74 62 27
BspEI T'CCGG_A
Cuts at: 2111 4778 2111
Size: 2667 2891
BspHI T'CATG_A
Cuts at: 449 2393 4734 449
Size: 1944 2341 1273
Fragments arranged by size:
2341 1944 1273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 797 2255 2541 2608 2822 3124 3505 797
Size: 1458 286 67 214 302 381 2850
Fragments arranged by size:
2850 1458 381 302 286 214 67
BsrBI CCG'CTC
Cuts at: 50 295 1101 1880 3850 3904 5388 5553 50
Size: 245 806 779 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 806 779 245 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1826 3470 1826
Size: 1644 3914
BsrFI r'CCGG_y
Cuts at: 1318 1760 3556 3737 3946 1318
Size: 442 1796 181 209 2930
Fragments arranged by size:
2930 1796 442 209 181
BssSI C'ACGA_G
Cuts at: 166 263 3829 4187 166
Size: 97 3566 358 1537
Fragments arranged by size:
3566 1537 358 97
BstAPI GCAn_nnn'nTGC
Cuts at: 2043 2935 2043
Size: 892 4666
BstBI TT'CG_AA
Cuts at: 246 3919 246
Size: 3673 1885
BstEII G'GTnAC_C
Cuts at: 306 1732 306
Size: 1426 4132
BstXI CCAn_nnnn'nTGG
Cuts at: 618 1869 618
Size: 1251 4307
BstYI r'GATC_y
Cuts at: 1 149 419 431 1670 1895 1997 2346 2583
Size: 148 270 12 1239 225 102 349 237
Cuts at: 2583 3050 3073 3408 3654 4666 4752 4764 5110
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5110 1
Size: 449
Fragments arranged by size:
1239 1012 467 449 349 346 335 270
246 237 225 148 102 86 23 12
12
Bsu36I CC'TnA_GG
Cuts at: 1182 2011 1182
Size: 829 4729
BtgI C'CryG_G
Cuts at: 107 210 686 815 824 1310 107
Size: 103 476 129 9 486 4355
Fragments arranged by size:
4355 486 476 129 103 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1035 1359 1446 1473 3508 3689 3852 1035
Size: 324 87 27 2035 181 163 2741
Fragments arranged by size:
2741 2035 324 181 163 87 27
BtsI GCAGTG_nn'
Cuts at: 640 1550 2801 640
Size: 910 1251 3397
Fragments arranged by size:
3397 1251 910
DraI TTT'AAA
Cuts at: 2557 2557
Size: 5558
DraIII CAC_nnn'GTG
Cuts at: 701 701
Size: 5558
DrdI GACnn_nn'nnGTC
Cuts at: 2251 3264 4122 2251
Size: 1013 858 3687
Fragments arranged by size:
3687 1013 858
EaeI y'GGCC_r
Cuts at: 812 1316 1722 3143 3317 3708 3735 5080 812
Size: 504 406 1421 174 391 27 1345 1290
Fragments arranged by size:
1421 1345 1290 504 406 391 174 27
EagI C'GGCC_G
Cuts at: 812 3143 812
Size: 2331 3227
EarI CTCTTCn'nnn_
Cuts at: 601 799 1108 1153 1369 1661 3581 3791 601
Size: 198 309 45 216 292 1920 210 2368
Fragments arranged by size:
2368 1920 309 292 216 210 198 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3766 4076 4222 5368 3766
Size: 310 146 1146 3956
Fragments arranged by size:
3956 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1146 2570 1146
Size: 1424 4134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1276 2155 2338 3383 3815 4562 4830 202
Size: 1074 879 183 1045 432 747 268 930
Fragments arranged by size:
1074 1045 930 879 747 432 268 183
EcoNI CCTnn'n_nnAGG
Cuts at: 544 937 544
Size: 393 5165
EcoO109I rG'GnC_Cy
Cuts at: 537 1473 2244 2697 537
Size: 936 771 453 3398
Fragments arranged by size:
3398 936 771 453
EcoRI G'AATT_C
Cuts at: 2562 2562
Size: 5558
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1542 3577 1542
Size: 2035 3523
FspI TGC'GCA
Cuts at: 406 3339 406
Size: 2933 2625
FspAI rTGC'GCAy
Cuts at: 406 406
Size: 5558
HaeII r_GCGC'y
Cuts at: 1174 1656 1809 2487 2992 3240 4262 5241 1174
Size: 482 153 678 505 248 1022 979 1491
Fragments arranged by size:
1491 1022 979 678 505 482 248 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1472 1504 2331 2363 2401 2433 3400 3430 3432
Size: 32 827 32 38 32 967 30 2
Cuts at: 3432 3462 1472
Size: 30 3568
Fragments arranged by size:
3568 967 827 38 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2597 2597
Size: 5558
KasI G'GCGC_C
Cuts at: 2988 3236 2988
Size: 248 5310
KpnI G_GTAC'C
Cuts at: 2578 2578
Size: 5558
MluI A'CGCG_T
Cuts at: 4991 4991
Size: 5558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 605 4229 4413 605
Size: 3624 184 1750
Fragments arranged by size:
3624 1750 184
MscI TGG'CCA
Cuts at: 1724 3319 1724
Size: 1595 3963
MslI CAynn'nnrTG
Cuts at: 400 860 2068 2395 3547 3674 400
Size: 460 1208 327 1152 127 2284
Fragments arranged by size:
2284 1208 1152 460 327 127
MspA1I CmG'CkG
Cuts at: 2040 2133 2669 2983 3343 4356 4601 2040
Size: 93 536 314 360 1013 245 2997
Fragments arranged by size:
2997 1013 536 360 314 245 93
NaeI GCC'GGC
Cuts at: 1320 3739 1320
Size: 2419 3139
NarI GG'CG_CC
Cuts at: 2989 3237 2989
Size: 248 5310
NcoI C'CATG_G
Cuts at: 107 210 107
Size: 103 5455
NgoMIV G'CCGG_C
Cuts at: 1318 3737 1318
Size: 2419 3139
NspI r_CATG'y
Cuts at: 2611 3111 4018 2611
Size: 500 907 4151
Fragments arranged by size:
4151 907 500
PasI CC'CwG_GG
Cuts at: 1014 1014
Size: 5558
PciI A'CATG_T
Cuts at: 4014 4014
Size: 5558
PflMI CCAn_nnn'nTGG
Cuts at: 1845 2178 1845
Size: 333 5225
PfoI T'CCnGG_A
Cuts at: 494 617 779 1511 1685 494
Size: 123 162 732 174 4367
Fragments arranged by size:
4367 732 174 162 123
PmeI GTTT'AAAC
Cuts at: 2557 2557
Size: 5558
PmlI CAC'GTG
Cuts at: 402 963 402
Size: 561 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 4697
Size: 32 5526
PpuMI rG'GwC_Cy
Cuts at: 2244 2244
Size: 5558
PshAI GACnn'nnGTC
Cuts at: 1345 1345
Size: 5558
PspOMI G'GGCC_C
Cuts at: 2201 2201
Size: 5558
PstI C_TGCA'G
Cuts at: 578 2605 3290 578
Size: 2027 685 2846
Fragments arranged by size:
2846 2027 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5558
PvuII CAG'CTG
Cuts at: 2133 2983 3343 2133
Size: 850 360 4348
Fragments arranged by size:
4348 850 360
RsrII CG'GwC_CG
Cuts at: 3753 3753
Size: 5558
SacI G_AGCT'C
Cuts at: 1148 2572 1148
Size: 1424 4134
SalI G'TCGA_C
Cuts at: 2595 2595
Size: 5558
SapI GCTCTTCn'nnn_
Cuts at: 799 1108 1153 1369 3581 3791 799
Size: 309 45 216 2212 210 2566
Fragments arranged by size:
2566 2212 309 216 210 45
SbfI CC_TGCA'GG
Cuts at: 2605 2605
Size: 5558
ScaI AGT'ACT
Cuts at: 5075 5075
Size: 5558
SexAI A'CCwGG_T
Cuts at: 1405 2300 1405
Size: 895 4663
SfcI C'TryA_G
Cuts at: 33 574 1116 2601 3286 4279 4470 33
Size: 541 542 1485 685 993 191 1121
Fragments arranged by size:
1485 1121 993 685 542 541 191
SfoI GGC'GCC
Cuts at: 2990 3238 2990
Size: 248 5310
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5558
SmaI CCC'GGG
Cuts at: 2580 2580
Size: 5558
SmlI C'TyrA_G
Cuts at: 483 2713 3628 4120 4382 483
Size: 2230 915 492 262 1659
Fragments arranged by size:
2230 1659 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5558
SphI G_CATG'C
Cuts at: 2611 3111 2611
Size: 500 5058
StuI AGG'CCT
Cuts at: 370 370
Size: 5558
StyI C'CwwG_G
Cuts at: 107 210 1445 1811 1979 2543 2692 3046 107
Size: 103 1235 366 168 564 149 354 2619
Fragments arranged by size:
2619 1235 564 366 354 168 149 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2202 3141 3274 3733 3942 2202
Size: 939 133 459 209 3818
Fragments arranged by size:
3818 939 459 209 133
TatI w'GTAC_w
Cuts at: 5073 5073
Size: 5558
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 279 311 554 586 1927 1959 5075 5107 279
Size: 32 243 32 1341 32 3116 32 730
Fragments arranged by size:
3116 1341 730 243 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 910 2415 2434 3355 910
Size: 1505 19 921 3113
Fragments arranged by size:
3113 1505 921 19
XbaI T'CTAG_A
Cuts at: 2589 2589
Size: 5558
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1608 1869 3053 1608
Size: 261 1184 4113
Fragments arranged by size:
4113 1184 261
XmaI C'CCGG_G
Cuts at: 2578 2578
Size: 5558
XmnI GAAnn'nnTTC
Cuts at: 709 1525 709
Size: 816 4742
ZraI GAC'GTC
Cuts at: 585 585
Size: 5558
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI