Restriction map for
pF1KE2689
(Circular) (Six-base) MAPSORT of: pF1KE2689.seq Check: 4671 from: 1 to: 4799
pF1KE2689 4799 bp
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 363 1084 363
Size: 721 4078
AatII G_ACGT'C
Cuts at: 928 1682 928
Size: 754 4045
AccI GT'mk_AC
Cuts at: 1837 1837
Size: 4799
Acc65I G'GTAC_C
Cuts at: 1815 1815
Size: 4799
AclI AA'CG_TT
Cuts at: 4639 4639
Size: 4799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 2624 3056 3803 4071 850
Size: 1774 432 747 268 1578
Fragments arranged by size:
1774 1578 747 432 268
AflIII A'CryG_T
Cuts at: 462 794 2151 3255 4232 462
Size: 332 1357 1104 977 1029
Fragments arranged by size:
1357 1104 1029 977 332
AgeI A'CCGG_T
Cuts at: 3187 3187
Size: 4799
AhdI GACnn_n'nnGTC
Cuts at: 924 924
Size: 4799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 964 964
Size: 4799
AlwNI CAG_nnn'CTG
Cuts at: 378 1252 2104 3671 378
Size: 874 852 1567 1506
Fragments arranged by size:
1567 1506 874 852
ApaI G_GGCC'C
Cuts at: 167 640 167
Size: 473 4326
ApaLI G'TGCA_C
Cuts at: 1192 3569 1192
Size: 2377 2422
ApoI r'AATT_y
Cuts at: 1803 4152 4408 1803
Size: 2349 256 2194
Fragments arranged by size:
2349 2194 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4799
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
AvaI C'yCGr_G
Cuts at: 613 804 1766 1819 613
Size: 191 962 53 3593
Fragments arranged by size:
3593 962 191 53
BamHI G'GATC_C
Cuts at: 1824 1824
Size: 4799
BanI G'GyrC_C
Cuts at: 331 409 618 1072 1815 2229 2477 2512 331
Size: 78 209 454 743 414 248 35 2618
Fragments arranged by size:
2618 743 454 414 248 209 78 35
BanII G_rGCy'C
Cuts at: 167 640 662 1502 1568 1813 2843 167
Size: 473 22 840 66 245 1030 2123
Fragments arranged by size:
2123 1030 840 473 245 66 22
BbeI G_GCGC'C
Cuts at: 2233 2481 2233
Size: 248 4551
BbsI GAAGACnn'nnnn_
Cuts at: 762 838 762
Size: 76 4723
BbvCI CC'TCA_GC
Cuts at: 451 1365 1666 451
Size: 914 301 3584
Fragments arranged by size:
3584 914 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1601 1635 90
Size: 34 1477 34 3254
Fragments arranged by size:
3254 1477 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2697 3458 4799 2697
Size: 761 1341 2697
Fragments arranged by size:
2697 1341 761
BclI T'GATC_A
Cuts at: 2319 2319
Size: 4799
BglI GCCn_nnn'nGGC
Cuts at: 508 1712 508
Size: 1204 3595
BglII A'GATC_T
Cuts at: 119 2314 4351 119
Size: 2195 2037 567
Fragments arranged by size:
2195 2037 567
BlpI GC'TnA_GC
Cuts at: 553 1911 553
Size: 1358 3441
Bme1580I G_kGCm'C
Cuts at: 167 623 640 1075 1196 2424 2517 3573 167
Size: 456 17 435 121 1228 93 1056 1393
Fragments arranged by size:
1393 1228 1056 456 435 121 93 17
BmrI ACTGGGnnnn_n'
Cuts at: 415 2011 2092 2427 4571 415
Size: 1596 81 335 2144 643
Fragments arranged by size:
2144 1596 643 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 3166 666
Size: 2500 2299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 724 1208 1582 167
Size: 557 484 374 3384
Fragments arranged by size:
3384 557 484 374
Bpu10I CC'TnA_GC
Cuts at: 451 1365 1666 451
Size: 914 301 3584
Fragments arranged by size:
3584 914 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1975 2854 3346 3644 1975
Size: 879 492 298 3130
Fragments arranged by size:
3130 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1456 4503 1456
Size: 3047 1752
BsaAI yAC'GTr
Cuts at: 465 2152 465
Size: 1687 3112
BsaBI GATnn'nnATC
Cuts at: 6 2313 4350 6
Size: 2307 2037 455
Fragments arranged by size:
2307 2037 455
BsaHI Gr'CG_yC
Cuts at: 925 1679 2230 2478 3180 4676 925
Size: 754 551 248 702 1496 1048
Fragments arranged by size:
1496 1048 754 702 551 248
BsaWI w'CCGG_w
Cuts at: 1988 2509 3187 3461 3608 4019 1988
Size: 521 678 274 147 411 2768
Fragments arranged by size:
2768 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 673 703 673
Size: 30 4769
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 244 132
Size: 112 4687
BseYI C'CCAG_C
Cuts at: 548 588 990 1354 2224 2982 3559 4055 548
Size: 40 402 364 870 758 577 496 1292
Fragments arranged by size:
1292 870 758 577 496 402 364 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 655 970 1265 303
Size: 352 315 295 3837
Fragments arranged by size:
3837 352 315 295
BsiEI CG_ry'CG
Cuts at: 104 823 2341 2387 3595 104
Size: 719 1518 46 1208 1308
Fragments arranged by size:
1518 1308 1208 719 46
BsiHKAI G_wGCw'C
Cuts at: 1196 1568 1813 2591 2781 3573 1196
Size: 372 245 778 190 792 2422
Fragments arranged by size:
2422 792 778 372 245 190
BsmI GAATG_Cn'
Cuts at: 282 282
Size: 4799
BsmBI CGTCTCn'nnnn_
Cuts at: 916 1147 916
Size: 231 4568
Bsp1286I G_dGCh'C
Cuts at: 167 623 640 662 1075 1196 1502 1568 1813
Size: 456 17 22 413 121 306 66 245
Cuts at: 1813 2424 2517 2591 2781 2843 3573 167
Size: 611 93 74 190 62 730 1393
Fragments arranged by size:
1393 730 611 456 413 306 245 190
121 93 74 66 62 22 17
BspEI T'CCGG_A
Cuts at: 4019 4019
Size: 4799
BspHI T'CATG_A
Cuts at: 3975 3975
Size: 4799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 363 677 905 1064 1084 1798 1849 2063 2365
Size: 314 228 159 20 714 51 214 302
Cuts at: 2365 2746 363
Size: 381 2416
Fragments arranged by size:
2416 714 381 314 302 228 214 159
51 20
BsrBI CCG'CTC
Cuts at: 50 3091 3145 4629 4794 50
Size: 3041 54 1484 165 55
Fragments arranged by size:
3041 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 861 2711 861
Size: 1850 2949
BsrFI r'CCGG_y
Cuts at: 698 2797 2978 3187 698
Size: 2099 181 209 2310
Fragments arranged by size:
2310 2099 209 181
BssSI C'ACGA_G
Cuts at: 3070 3428 3070
Size: 358 4441
BstAPI GCAn_nnn'nTGC
Cuts at: 409 1252 2176 409
Size: 843 924 3032
Fragments arranged by size:
3032 924 843
BstBI TT'CG_AA
Cuts at: 932 3160 932
Size: 2228 2571
BstXI CCAn_nnnn'nTGG
Cuts at: 297 498 1377 297
Size: 201 879 3719
Fragments arranged by size:
3719 879 201
BstYI r'GATC_y
Cuts at: 1 119 953 1248 1349 1824 2291 2314 2649
Size: 118 834 295 101 475 467 23 335
Cuts at: 2649 2895 3907 3993 4005 4351 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 834 475 467 449 346 335 295
246 118 101 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 641 641
Size: 4799
BtgI C'CryG_G
Cuts at: 290 327 491 823 1091 1340 290
Size: 37 164 332 268 249 3749
Fragments arranged by size:
3749 332 268 249 164 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2749 2930 3093 2749
Size: 181 163 4455
Fragments arranged by size:
4455 181 163
BtsI GCAGTG_nn'
Cuts at: 263 1130 1650 2042 263
Size: 867 520 392 3020
Fragments arranged by size:
3020 867 520 392
DraI TTT'AAA
Cuts at: 1798 1798
Size: 4799
DraIII CAC_nnn'GTG
Cuts at: 409 409
Size: 4799
DrdI GACnn_nn'nnGTC
Cuts at: 2505 3363 2505
Size: 858 3941
EaeI y'GGCC_r
Cuts at: 485 820 1379 2384 2558 2949 2976 4321 485
Size: 335 559 1005 174 391 27 1345 963
Fragments arranged by size:
1345 1005 963 559 391 335 174 27
EagI C'GGCC_G
Cuts at: 820 2384 820
Size: 1564 3235
EarI CTCTTCn'nnn_
Cuts at: 221 233 2822 3032 221
Size: 12 2589 210 1988
Fragments arranged by size:
2589 1988 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3007 3317 3463 4609 3007
Size: 310 146 1146 3197
Fragments arranged by size:
3197 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1566 1811 1566
Size: 245 4554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 724 850 1208 1582 2624 3056 3803 4071
Size: 557 126 358 374 1042 432 747 268
Cuts at: 4071 167
Size: 895
Fragments arranged by size:
1042 895 747 557 432 374 358 268
126
EcoNI CCTnn'n_nnAGG
Cuts at: 421 421
Size: 4799
EcoO109I rG'GnC_Cy
Cuts at: 163 164 184 637 739 1938 163
Size: 1 20 453 102 1199 3024
Fragments arranged by size:
3024 1199 453 102 20 1
EcoRI G'AATT_C
Cuts at: 1803 1803
Size: 4799
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4799
FspI TGC'GCA
Cuts at: 2580 2580
Size: 4799
HaeII r_GCGC'y
Cuts at: 2233 2481 3503 4482 2233
Size: 248 1022 979 2550
Fragments arranged by size:
2550 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 910 942 945 977 2641 2671 2673 2703 910
Size: 32 3 32 1664 30 2 30 3006
Fragments arranged by size:
3006 1664 32 32 30 30 3 2
HincII GTy'rAC
Cuts at: 1838 1838
Size: 4799
KasI G'GCGC_C
Cuts at: 2229 2477 2229
Size: 248 4551
KpnI G_GTAC'C
Cuts at: 1819 1819
Size: 4799
MluI A'CGCG_T
Cuts at: 4232 4232
Size: 4799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3470 3654 3470
Size: 184 4615
MscI TGG'CCA
Cuts at: 487 1381 2560 487
Size: 894 1179 2726
Fragments arranged by size:
2726 1179 894
MslI CAynn'nnrTG
Cuts at: 346 946 1426 2788 2915 346
Size: 600 480 1362 127 2230
Fragments arranged by size:
2230 1362 600 480 127
MspA1I CmG'CkG
Cuts at: 317 825 990 1044 1910 2224 2584 3597 3842
Size: 508 165 54 866 314 360 1013 245
Cuts at: 3842 317
Size: 1274
Fragments arranged by size:
1274 1013 866 508 360 314 245 165
54
NaeI GCC'GGC
Cuts at: 2980 2980
Size: 4799
NarI GG'CG_CC
Cuts at: 2230 2478 2230
Size: 248 4551
NcoI C'CATG_G
Cuts at: 290 327 1091 1340 290
Size: 37 764 249 3749
Fragments arranged by size:
3749 764 249 37
NgoMIV G'CCGG_C
Cuts at: 2978 2978
Size: 4799
NotI GC'GGCC_GC
Cuts at: 820 820
Size: 4799
NspI r_CATG'y
Cuts at: 714 1200 1852 2352 3259 714
Size: 486 652 500 907 2254
Fragments arranged by size:
2254 907 652 500 486
PasI CC'CwG_GG
Cuts at: 160 1067 160
Size: 907 3892
PciI A'CATG_T
Cuts at: 3255 3255
Size: 4799
PflMI CCAn_nnn'nTGG
Cuts at: 702 702
Size: 4799
PmeI GTTT'AAAC
Cuts at: 1798 1798
Size: 4799
PmlI CAC'GTG
Cuts at: 465 465
Size: 4799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4767
PpuMI rG'GwC_Cy
Cuts at: 184 739 184
Size: 555 4244
PspOMI G'GGCC_C
Cuts at: 163 636 163
Size: 473 4326
PstI C_TGCA'G
Cuts at: 1846 2531 1846
Size: 685 4114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4799
PvuII CAG'CTG
Cuts at: 317 990 2224 2584 317
Size: 673 1234 360 2532
Fragments arranged by size:
2532 1234 673 360
RsrII CG'GwC_CG
Cuts at: 2994 2994
Size: 4799
SacI G_AGCT'C
Cuts at: 1568 1813 1568
Size: 245 4554
SacII CC_GC'GG
Cuts at: 826 826
Size: 4799
SalI G'TCGA_C
Cuts at: 1836 1836
Size: 4799
SanDI GG'GwC_CC
Cuts at: 184 739 184
Size: 555 4244
SapI GCTCTTCn'nnn_
Cuts at: 2822 3032 2822
Size: 210 4589
SbfI CC_TGCA'GG
Cuts at: 1846 1846
Size: 4799
ScaI AGT'ACT
Cuts at: 1288 4316 1288
Size: 3028 1771
SexAI A'CCwGG_T
Cuts at: 1163 1163
Size: 4799
SfcI C'TryA_G
Cuts at: 33 1122 1842 2527 3520 3711 33
Size: 1089 720 685 993 191 1121
Fragments arranged by size:
1121 1089 993 720 685 191
SfoI GGC'GCC
Cuts at: 2231 2479 2231
Size: 248 4551
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
SmaI CCC'GGG
Cuts at: 615 1821 615
Size: 1206 3593
SmlI C'TyrA_G
Cuts at: 1954 2869 3361 3623 1954
Size: 915 492 262 3130
Fragments arranged by size:
3130 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4799
SphI G_CATG'C
Cuts at: 1852 2352 1852
Size: 500 4299
StuI AGG'CCT
Cuts at: 646 1483 646
Size: 837 3962
StyI C'CwwG_G
Cuts at: 167 290 327 367 732 1091 1340 1933 2287
Size: 123 37 40 365 359 249 593 354
Cuts at: 2287 167
Size: 2679
Fragments arranged by size:
2679 593 365 359 354 249 123 40
37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 303 397 1495 2382 2515 2974 3183 303
Size: 94 1098 887 133 459 209 1919
Fragments arranged by size:
1919 1098 887 459 209 133 94
TatI w'GTAC_w
Cuts at: 1219 1232 1286 4314 1219
Size: 13 54 3028 1704
Fragments arranged by size:
3028 1704 54 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4316 4348 4316
Size: 32 4767
Tth111I GACn'n_nGTC
Cuts at: 1678 2596 1678
Size: 918 3881
XbaI T'CTAG_A
Cuts at: 1830 1830
Size: 4799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 495 591 994 2294 297
Size: 198 96 403 1300 2802
Fragments arranged by size:
2802 1300 403 198 96
XmaI C'CCGG_G
Cuts at: 613 1819 613
Size: 1206 3593
ZraI GAC'GTC
Cuts at: 926 1680 926
Size: 754 4045
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI AscI AvrII BaeI BfrBI
BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PfoI PshAI PsiI PspXI
PsrI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI