Restriction map for
pF1KE2680
(Circular) (Six-base) MAPSORT of: pF1KE2680.seq Check: 3521 from: 1 to: 4394
pF1KE2680 4394 bp
With 182 enzymes: SgfI *
March 1, 2017 16:47 ..
AatII G_ACGT'C
Cuts at: 359 1313 359
Size: 954 3440
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 642 2219 2651 3398 3666 280
Size: 362 1577 432 747 268 1008
Fragments arranged by size:
1577 1008 747 432 362 268
AfeI AGC'GCT
Cuts at: 508 1000 508
Size: 492 3902
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 1319 2782 1319
Size: 1463 2931
AleI CACnn'nnGTG
Cuts at: 1273 1273
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 1699 3266 1699
Size: 1567 2827
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 621 1232 1414 621
Size: 611 182 3601
Fragments arranged by size:
3601 611 182
BamHI G'GATC_C
Cuts at: 214 837 1121 1419 214
Size: 623 284 298 3189
Fragments arranged by size:
3189 623 298 284
BanI G'GyrC_C
Cuts at: 1249 1410 1824 2072 2107 1249
Size: 161 414 248 35 3536
Fragments arranged by size:
3536 414 248 161 35
BanII G_rGCy'C
Cuts at: 164 272 1408 2438 164
Size: 108 1136 1030 2120
Fragments arranged by size:
2120 1136 1030 108
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 390 390
Size: 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1273 1307 1273
Size: 34 4360
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglII A'GATC_T
Cuts at: 743 1909 3946 743
Size: 1166 2037 1191
Fragments arranged by size:
2037 1191 1166
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 2019 2112 3168 2019
Size: 93 1056 3245
Fragments arranged by size:
3245 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 979 730
Size: 249 4145
Bpu10I CC'TnA_GC
Cuts at: 796 796
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 1570 2449 2941 3239 158
Size: 1412 879 492 298 1313
Fragments arranged by size:
1412 1313 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 562 859 4098 562
Size: 297 3239 858
Fragments arranged by size:
3239 858 297
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1159 1908 3945 6
Size: 1153 749 2037 455
Fragments arranged by size:
2037 1153 749 455
BsaHI Gr'CG_yC
Cuts at: 356 1310 1825 2073 2775 4271 356
Size: 954 515 248 702 1496 479
Fragments arranged by size:
1496 954 702 515 479 248
BsaWI w'CCGG_w
Cuts at: 1319 1583 2104 2782 3056 3203 3614 1319
Size: 264 521 678 274 147 411 2099
Fragments arranged by size:
2099 678 521 411 274 264 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 174 204 1069 1099 174
Size: 30 865 30 3469
Fragments arranged by size:
3469 865 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 161 1012 1090 161
Size: 851 78 3465
Fragments arranged by size:
3465 851 78
BseYI C'CCAG_C
Cuts at: 526 1016 1819 2577 3154 3650 526
Size: 490 803 758 577 496 1270
Fragments arranged by size:
1270 803 758 577 496 490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 840 865 1015 1204 757
Size: 83 25 150 189 3947
Fragments arranged by size:
3947 189 150 83 25
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 184 272 1408 2186 2376 3168 184
Size: 88 1136 778 190 792 1410
Fragments arranged by size:
1410 1136 792 778 190 88
BsmI GAATG_Cn'
Cuts at: 329 329
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 164 184 272 1408 2019 2112 2186 2376 2438
Size: 20 88 1136 611 93 74 190 62
Cuts at: 2438 3168 164
Size: 730 1390
Fragments arranged by size:
1390 1136 730 611 190 93 88 74
62 20
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1226 1444 1658 1960 2341 1226
Size: 218 214 302 381 3279
Fragments arranged by size:
3279 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 1319 2392 2573 2782 1319
Size: 1073 181 209 2931
Fragments arranged by size:
2931 1073 209 181
BssSI C'ACGA_G
Cuts at: 468 640 763 1024 2665 3023 468
Size: 172 123 261 1641 358 1839
Fragments arranged by size:
1839 1641 358 261 172 123
BstAPI GCAn_nnn'nTGC
Cuts at: 684 888 1771 684
Size: 204 883 3307
Fragments arranged by size:
3307 883 204
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 238 238
Size: 4394
BstYI r'GATC_y
Cuts at: 1 214 743 837 1121 1419 1886 1909 2244
Size: 213 529 94 284 298 467 23 335
Cuts at: 2244 2490 3502 3588 3600 3946 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 529 467 449 346 335 298 284
246 213 94 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1199 1199
Size: 4394
BtgI C'CryG_G
Cuts at: 107 842 107
Size: 735 3659
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1032 2344 2525 2688 1032
Size: 1312 181 163 2738
Fragments arranged by size:
2738 1312 181 163
BtsI GCAGTG_nn'
Cuts at: 778 1637 778
Size: 859 3535
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DraIII CAC_nnn'GTG
Cuts at: 684 1227 684
Size: 543 3851
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 407 1043 1130 1146 1979 2153 2544 2571 3916
Size: 636 87 16 833 174 391 27 1345
Cuts at: 3916 407
Size: 885
Fragments arranged by size:
1345 885 833 636 391 174 87 27
16
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 146 152 2417 2627 146
Size: 6 2265 210 1913
Fragments arranged by size:
2265 1913 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 739 1134 2602 2912 3058 4204 739
Size: 395 1468 310 146 1146 929
Fragments arranged by size:
1468 1146 929 395 310 146
EcoICRI GAG'CTC
Cuts at: 270 1406 270
Size: 1136 3258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 642 730 979 2219 2651 3398 3666 280
Size: 362 88 249 1240 432 747 268 1008
Fragments arranged by size:
1240 1008 747 432 362 268 249 88
EcoNI CCTnn'n_nnAGG
Cuts at: 364 799 364
Size: 435 3959
EcoO109I rG'GnC_Cy
Cuts at: 617 1533 617
Size: 916 3478
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 510 640 791 1002 1083 1828 2076 3098 4077
Size: 130 151 211 81 745 248 1022 979
Cuts at: 4077 510
Size: 827
Fragments arranged by size:
1022 979 827 745 248 211 151 130
81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1069 1101 1160 1192 2236 2266 2268 2298 1069
Size: 32 59 32 1044 30 2 30 3165
Fragments arranged by size:
3165 1044 59 32 32 30 30 2
HincII GTy'rAC
Cuts at: 435 1173 1433 435
Size: 738 260 3396
Fragments arranged by size:
3396 738 260
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3249 3065
Size: 184 4210
MscI TGG'CCA
Cuts at: 409 1132 2155 409
Size: 723 1023 2648
Fragments arranged by size:
2648 1023 723
MslI CAynn'nnrTG
Cuts at: 847 1273 2383 2510 847
Size: 426 1110 127 2731
Fragments arranged by size:
2731 1110 426 127
MspA1I CmG'CkG
Cuts at: 394 717 1116 1505 1819 2179 3192 3437 394
Size: 323 399 389 314 360 1013 245 1351
Fragments arranged by size:
1351 1013 399 389 360 323 314 245
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 107 842 107
Size: 735 3659
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 525 726 1197 1447 1947 2854 525
Size: 201 471 250 500 907 2065
Fragments arranged by size:
2065 907 500 471 250 201
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 609 609
Size: 4394
PfoI T'CCnGG_A
Cuts at: 245 376 1313 245
Size: 131 937 3326
Fragments arranged by size:
3326 937 131
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 617 617
Size: 4394
PshAI GACnn'nnGTC
Cuts at: 394 394
Size: 4394
PstI C_TGCA'G
Cuts at: 893 1441 2126 893
Size: 548 685 3161
Fragments arranged by size:
3161 685 548
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 717 1819 2179 717
Size: 1102 360 2932
Fragments arranged by size:
2932 1102 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 272 1408 272
Size: 1136 3258
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SanDI GG'GwC_CC
Cuts at: 617 617
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 152 2417 2627 152
Size: 2265 210 1919
Fragments arranged by size:
2265 1919 210
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 898 3911 898
Size: 3013 1381
SexAI A'CCwGG_T
Cuts at: 968 968
Size: 4394
SfcI C'TryA_G
Cuts at: 33 415 889 1437 2122 3115 3306 33
Size: 382 474 548 685 993 191 1121
Fragments arranged by size:
1121 993 685 548 474 382 191
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 623 1234 1416 623
Size: 611 182 3601
Fragments arranged by size:
3601 611 182
SmlI C'TyrA_G
Cuts at: 173 1549 2464 2956 3218 173
Size: 1376 915 492 262 1349
Fragments arranged by size:
1376 1349 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
SrfI GCCC'GGGC
Cuts at: 1234 1234
Size: 4394
StuI AGG'CCT
Cuts at: 1259 1259
Size: 4394
StyI C'CwwG_G
Cuts at: 107 842 1528 1882 107
Size: 735 686 354 2619
Fragments arranged by size:
2619 735 686 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1977 2110 2569 2778 1977
Size: 133 459 209 3593
Fragments arranged by size:
3593 459 209 133
TatI w'GTAC_w
Cuts at: 518 896 3909 518
Size: 378 3013 1003
Fragments arranged by size:
3013 1003 378
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 174 206 346 378 3911 3943 174
Size: 32 140 32 3533 32 625
Fragments arranged by size:
3533 625 140 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 348 378 1889 348
Size: 30 1511 2853
Fragments arranged by size:
2853 1511 30
XmaI C'CCGG_G
Cuts at: 621 1232 1414 621
Size: 611 182 3601
Fragments arranged by size:
3601 611 182
ZraI GAC'GTC
Cuts at: 357 1311 357
Size: 954 3440
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SspI SwaI XhoI XmnI