Restriction map for
pF1KE2674
(Circular) (Six-base) MAPSORT of: pF1KE2674.seq Check: 8190 from: 1 to: 6056
pF1KE2674 6056 bp
With 182 enzymes: SgfI *
February 27, 2017 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1941 2063 1941
Size: 122 5934
AccI GT'mk_AC
Cuts at: 3094 3094
Size: 6056
Acc65I G'GTAC_C
Cuts at: 2167 3072 2167
Size: 905 5151
AclI AA'CG_TT
Cuts at: 5896 5896
Size: 6056
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 2858 3881 4313 5060 5328 293
Size: 2565 1023 432 747 268 1021
Fragments arranged by size:
2565 1023 1021 747 432 268
AflIII A'CryG_T
Cuts at: 1933 3408 4512 5489 1933
Size: 1475 1104 977 2500
Fragments arranged by size:
2500 1475 1104 977
AgeI A'CCGG_T
Cuts at: 4444 4444
Size: 6056
AleI CACnn'nnGTG
Cuts at: 2047 2047
Size: 6056
AlwNI CAG_nnn'CTG
Cuts at: 3361 4928 3361
Size: 1567 4489
ApaLI G'TGCA_C
Cuts at: 2256 4826 2256
Size: 2570 3486
ApoI r'AATT_y
Cuts at: 527 666 1451 1477 3060 5409 5665 527
Size: 139 785 26 1583 2349 256 918
Fragments arranged by size:
2349 1583 918 785 256 139 26
AseI AT'TA_AT
Cuts at: 20 718 20
Size: 698 5358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6056
AvaI C'yCGr_G
Cuts at: 329 2325 3076 329
Size: 1996 751 3309
Fragments arranged by size:
3309 1996 751
BamHI G'GATC_C
Cuts at: 3081 3081
Size: 6056
BanI G'GyrC_C
Cuts at: 2167 3072 3486 3734 3769 2167
Size: 905 414 248 35 4454
Fragments arranged by size:
4454 905 414 248 35
BanII G_rGCy'C
Cuts at: 1574 3070 4100 1574
Size: 1496 1030 3530
Fragments arranged by size:
3530 1496 1030
BbeI G_GCGC'C
Cuts at: 3490 3738 3490
Size: 248 5808
BbsI GAAGACnn'nnnn_
Cuts at: 2918 2918
Size: 6056
BciVI GTATCCnnnnn_n'
Cuts at: 2570 2733 3954 4715 6056 2570
Size: 163 1221 761 1341 2570
Fragments arranged by size:
2570 1341 1221 761 163
BclI T'GATC_A
Cuts at: 513 1755 3576 513
Size: 1242 1821 2993
Fragments arranged by size:
2993 1821 1242
BglII A'GATC_T
Cuts at: 2103 3571 5608 2103
Size: 1468 2037 2551
Fragments arranged by size:
2551 2037 1468
BlpI GC'TnA_GC
Cuts at: 3168 3168
Size: 6056
Bme1580I G_kGCm'C
Cuts at: 2260 2604 3681 3774 4830 2260
Size: 344 1077 93 1056 3486
Fragments arranged by size:
3486 1077 1056 344 93
BmgBI CAC'GTC
Cuts at: 1134 1134
Size: 6056
BmrI ACTGGGnnnn_n'
Cuts at: 2358 2579 3268 3349 3684 5828 2358
Size: 221 689 81 335 2144 2586
Fragments arranged by size:
2586 2144 689 335 221 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4423 4423
Size: 6056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 910 1440 1544 1703 2896 3040 572
Size: 338 530 104 159 1193 144 3588
Fragments arranged by size:
3588 1193 530 338 159 144 104
Bpu10I CC'TnA_GC
Cuts at: 1940 1940
Size: 6056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 291 3232 4111 4603 4901 208
Size: 83 2941 879 492 298 1363
Fragments arranged by size:
2941 1363 879 492 298 83
BsaI GGTCTCn'nnnn_
Cuts at: 2942 5760 2942
Size: 2818 3238
BsaAI yAC'GTr
Cuts at: 3409 3409
Size: 6056
BsaBI GATnn'nnATC
Cuts at: 6 1721 1727 2030 3570 5607 6
Size: 1715 6 303 1540 2037 455
Fragments arranged by size:
2037 1715 1540 455 303 6
BsaHI Gr'CG_yC
Cuts at: 258 3487 3735 4437 5933 258
Size: 3229 248 702 1496 381
Fragments arranged by size:
3229 1496 702 381 248
BsaWI w'CCGG_w
Cuts at: 3245 3766 4444 4718 4865 5276 3245
Size: 521 678 274 147 411 4025
Fragments arranged by size:
4025 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 551 581 2793 2823 551
Size: 30 2212 30 3784
Fragments arranged by size:
3784 2212 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1015 1700 2938 2941 1015
Size: 685 1238 3 4130
Fragments arranged by size:
4130 1238 685 3
BseYI C'CCAG_C
Cuts at: 548 3481 4239 4816 5312 548
Size: 2933 758 577 496 1292
Fragments arranged by size:
2933 1292 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 2042 1504
Size: 538 5518
BsiEI CG_ry'CG
Cuts at: 104 807 950 2389 3598 3644 4852 104
Size: 703 143 1439 1209 46 1208 1308
Fragments arranged by size:
1439 1308 1209 1208 703 143 46
BsiHKAI G_wGCw'C
Cuts at: 1941 2260 3070 3848 4038 4830 1941
Size: 319 810 778 190 792 3167
Fragments arranged by size:
3167 810 792 778 319 190
BsmI GAATG_Cn'
Cuts at: 606 1495 2064 606
Size: 889 569 4598
Fragments arranged by size:
4598 889 569
BsmBI CGTCTCn'nnnn_
Cuts at: 2769 2769
Size: 6056
Bsp1286I G_dGCh'C
Cuts at: 1574 1941 2260 2604 3070 3681 3774 3848 4038
Size: 367 319 344 466 611 93 74 190
Cuts at: 4038 4100 4830 1574
Size: 62 730 2800
Fragments arranged by size:
2800 730 611 466 367 344 319 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5276 5276
Size: 6056
BspHI T'CATG_A
Cuts at: 1629 1719 2190 5232 1629
Size: 90 471 3042 2453
Fragments arranged by size:
3042 2453 471 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1941 2063 3106 3320 3622 4003 1941
Size: 122 1043 214 302 381 3994
Fragments arranged by size:
3994 1043 381 302 214 122
BsrBI CCG'CTC
Cuts at: 50 1424 4348 4402 5886 6051 50
Size: 1374 2924 54 1484 165 55
Fragments arranged by size:
2924 1484 1374 165 55 54
BsrDI GCAATG_nn'
Cuts at: 968 3968 968
Size: 3000 3056
BsrFI r'CCGG_y
Cuts at: 121 4054 4235 4444 121
Size: 3933 181 209 1733
Fragments arranged by size:
3933 1733 209 181
BssSI C'ACGA_G
Cuts at: 194 4327 4685 194
Size: 4133 358 1565
Fragments arranged by size:
4133 1565 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3433 3433
Size: 6056
BstBI TT'CG_AA
Cuts at: 4417 4417
Size: 6056
BstXI CCAn_nnnn'nTGG
Cuts at: 2874 3018 2874
Size: 144 5912
BstYI r'GATC_y
Cuts at: 1 2103 2185 2464 3081 3548 3571 3906 4152
Size: 2102 82 279 617 467 23 335 246
Cuts at: 4152 5164 5250 5262 5608 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2102 1012 617 467 449 346 335 279
246 86 82 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6056
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4006 4187 4350 4006
Size: 181 163 5712
Fragments arranged by size:
5712 181 163
BtsI GCAGTG_nn'
Cuts at: 1165 1492 1644 3299 1165
Size: 327 152 1655 3922
Fragments arranged by size:
3922 1655 327 152
ClaI AT'CG_AT
Cuts at: 2385 2385
Size: 6056
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 348 383 348
Size: 35 6021
DraI TTT'AAA
Cuts at: 3055 3055
Size: 6056
DrdI GACnn_nn'nnGTC
Cuts at: 3762 4620 3762
Size: 858 5198
EaeI y'GGCC_r
Cuts at: 808 2051 2455 3641 3815 4206 4233 5578 808
Size: 1243 404 1186 174 391 27 1345 1286
Fragments arranged by size:
1345 1286 1243 1186 404 391 174 27
EagI C'GGCC_G
Cuts at: 3641 3641
Size: 6056
EarI CTCTTCn'nnn_
Cuts at: 561 2772 2834 2930 2966 4079 4289 561
Size: 2211 62 96 36 1113 210 2328
Fragments arranged by size:
2328 2211 1113 210 96 62 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4264 4574 4720 5866 4264
Size: 310 146 1146 4454
Fragments arranged by size:
4454 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3068 3068
Size: 6056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 572 910 1440 1544 1703 2858 2896 3040
Size: 279 338 530 104 159 1155 38 144
Cuts at: 3040 3881 4313 5060 5328 293
Size: 841 432 747 268 1021
Fragments arranged by size:
1155 1021 841 747 530 432 338 279
268 159 144 104 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1583 3019 1583
Size: 1436 4620
EcoO109I rG'GnC_Cy
Cuts at: 1588 3195 1588
Size: 1607 4449
EcoRI G'AATT_C
Cuts at: 3060 3060
Size: 6056
EcoRV GAT'ATC
Cuts at: 2481 2657 2481
Size: 176 5880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 442 3031 4075 442
Size: 2589 1044 2423
Fragments arranged by size:
2589 2423 1044
FspI TGC'GCA
Cuts at: 3837 3837
Size: 6056
HaeII r_GCGC'y
Cuts at: 3490 3738 4760 5739 3490
Size: 248 1022 979 3807
Fragments arranged by size:
3807 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 1923 1955 3898 3928 3930 3960 163
Size: 32 1728 32 1943 30 2 30 2259
Fragments arranged by size:
2259 1943 1728 32 32 30 30 2
HincII GTy'rAC
Cuts at: 840 3095 840
Size: 2255 3801
KasI G'GCGC_C
Cuts at: 3486 3734 3486
Size: 248 5808
KpnI G_GTAC'C
Cuts at: 2171 3076 2171
Size: 905 5151
MfeI C'AATT_G
Cuts at: 407 407
Size: 6056
MluI A'CGCG_T
Cuts at: 5489 5489
Size: 6056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 970 4727 4911 970
Size: 3757 184 2115
Fragments arranged by size:
3757 2115 184
MscI TGG'CCA
Cuts at: 2053 2457 3817 2053
Size: 404 1360 4292
Fragments arranged by size:
4292 1360 404
MslI CAynn'nnrTG
Cuts at: 395 509 755 1247 1883 2036 2047 4045 4172
Size: 114 246 492 636 153 11 1998 127
Cuts at: 4172 395
Size: 2279
Fragments arranged by size:
2279 1998 636 492 246 153 127 114
11
MspA1I CmG'CkG
Cuts at: 640 2836 2945 3167 3481 3841 4854 5099 640
Size: 2196 109 222 314 360 1013 245 1597
Fragments arranged by size:
2196 1597 1013 360 314 245 222 109
NaeI GCC'GGC
Cuts at: 123 4237 123
Size: 4114 1942
NarI GG'CG_CC
Cuts at: 3487 3735 3487
Size: 248 5808
NcoI C'CATG_G
Cuts at: 107 107
Size: 6056
NdeI CA'TA_TG
Cuts at: 900 1274 900
Size: 374 5682
NgoMIV G'CCGG_C
Cuts at: 121 4235 121
Size: 4114 1942
NspI r_CATG'y
Cuts at: 1391 1937 3109 3609 4516 1391
Size: 546 1172 500 907 2931
Fragments arranged by size:
2931 1172 907 546 500
PciI A'CATG_T
Cuts at: 1933 4512 1933
Size: 2579 3477
PmeI GTTT'AAAC
Cuts at: 3055 3055
Size: 6056
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5195 5227 5195
Size: 32 6024
PpuMI rG'GwC_Cy
Cuts at: 1588 1588
Size: 6056
PspXI vC'TCGA_Gb
Cuts at: 329 329
Size: 6056
PstI C_TGCA'G
Cuts at: 744 1172 3103 3788 744
Size: 428 1931 685 3012
Fragments arranged by size:
3012 1931 685 428
PvuI CG_AT'CG
Cuts at: 104 2389 104
Size: 2285 3771
PvuII CAG'CTG
Cuts at: 640 2836 3481 3841 640
Size: 2196 645 360 2855
Fragments arranged by size:
2855 2196 645 360
RsrII CG'GwC_CG
Cuts at: 4251 4251
Size: 6056
SacI G_AGCT'C
Cuts at: 3070 3070
Size: 6056
SalI G'TCGA_C
Cuts at: 3093 3093
Size: 6056
SapI GCTCTTCn'nnn_
Cuts at: 2966 4079 4289 2966
Size: 1113 210 4733
Fragments arranged by size:
4733 1113 210
SbfI CC_TGCA'GG
Cuts at: 3103 3103
Size: 6056
ScaI AGT'ACT
Cuts at: 156 986 5573 156
Size: 830 4587 639
Fragments arranged by size:
4587 830 639
SfcI C'TryA_G
Cuts at: 33 740 921 1168 1565 1778 2180 2732 3099
Size: 707 181 247 397 213 402 552 367
Cuts at: 3099 3784 4777 4968 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 707 685 552 402 397 367
247 213 191 181
SfoI GGC'GCC
Cuts at: 3488 3736 3488
Size: 248 5808
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6056
SmaI CCC'GGG
Cuts at: 3078 3078
Size: 6056
SmlI C'TyrA_G
Cuts at: 187 306 329 3211 4126 4618 4880 187
Size: 119 23 2882 915 492 262 1363
Fragments arranged by size:
2882 1363 915 492 262 119 23
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6056
SphI G_CATG'C
Cuts at: 3109 3609 3109
Size: 500 5556
SspI AAT'ATT
Cuts at: 2342 2342
Size: 6056
StuI AGG'CCT
Cuts at: 1119 1119
Size: 6056
StyI C'CwwG_G
Cuts at: 107 2952 3190 3544 107
Size: 2845 238 354 2619
Fragments arranged by size:
2845 2619 354 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 726 1376 3639 3772 4231 4440 726
Size: 650 2263 133 459 209 2342
Fragments arranged by size:
2342 2263 650 459 209 133
TatI w'GTAC_w
Cuts at: 154 984 1869 2345 2594 5571 154
Size: 830 885 476 249 2977 639
Fragments arranged by size:
2977 885 830 639 476 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 551 583 2793 2825 5573 5605 551
Size: 32 2210 32 2748 32 1002
Fragments arranged by size:
2748 2210 1002 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3853 3853
Size: 6056
XbaI T'CTAG_A
Cuts at: 3087 3087
Size: 6056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3551 3551
Size: 6056
XhoI C'TCGA_G
Cuts at: 329 329
Size: 6056
XmaI C'CCGG_G
Cuts at: 3076 3076
Size: 6056
XmnI GAAnn'nnTTC
Cuts at: 2064 2064
Size: 6056
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I DraIII FseI FspAI
HindIII HpaI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI