Restriction map for
pF1KE2673
(Circular) (Six-base) MAPSORT of: pF1KE2673.seq Check: 567 from: 1 to: 5492
pF1KE2673 5492 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1885 1885
Size: 5492
AccI GT'mk_AC
Cuts at: 1328 1657 2530 1328
Size: 329 873 4290
Fragments arranged by size:
4290 873 329
Acc65I G'GTAC_C
Cuts at: 2508 2508
Size: 5492
AclI AA'CG_TT
Cuts at: 5332 5332
Size: 5492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 488 3317 3749 4496 4764 229
Size: 259 2829 432 747 268 957
Fragments arranged by size:
2829 957 747 432 268 259
AflIII A'CryG_T
Cuts at: 2844 3948 4925 2844
Size: 1104 977 3411
Fragments arranged by size:
3411 1104 977
AgeI A'CCGG_T
Cuts at: 3880 3880
Size: 5492
AlwNI CAG_nnn'CTG
Cuts at: 1098 2797 4364 1098
Size: 1699 1567 2226
Fragments arranged by size:
2226 1699 1567
ApaLI G'TGCA_C
Cuts at: 4262 4262
Size: 5492
ApoI r'AATT_y
Cuts at: 551 626 865 939 1023 1264 1494 1723 2271
Size: 75 239 74 84 241 230 229 548
Cuts at: 2271 2416 2496 4845 5101 551
Size: 145 80 2349 256 942
Fragments arranged by size:
2349 942 548 256 241 239 230 229
145 84 80 75 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 5492
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5492
AvaI C'yCGr_G
Cuts at: 126 141 2096 2512 126
Size: 15 1955 416 3106
Fragments arranged by size:
3106 1955 416 15
BamHI G'GATC_C
Cuts at: 801 2517 801
Size: 1716 3776
BanI G'GyrC_C
Cuts at: 145 1873 2508 2922 3170 3205 145
Size: 1728 635 414 248 35 2432
Fragments arranged by size:
2432 1728 635 414 248 35
BanII G_rGCy'C
Cuts at: 2506 3536 2506
Size: 1030 4462
BbeI G_GCGC'C
Cuts at: 149 2926 3174 149
Size: 2777 248 2467
Fragments arranged by size:
2777 2467 248
BbsI GAAGACnn'nnnn_
Cuts at: 1457 2081 1457
Size: 624 4868
BciVI GTATCCnnnnn_n'
Cuts at: 3390 4151 5492 3390
Size: 761 1341 3390
Fragments arranged by size:
3390 1341 761
BclI T'GATC_A
Cuts at: 3012 3012
Size: 5492
BfrBI ATG'CAT
Cuts at: 1579 1579
Size: 5492
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5492
BglII A'GATC_T
Cuts at: 2406 3007 5044 2406
Size: 601 2037 2854
Fragments arranged by size:
2854 2037 601
BlpI GC'TnA_GC
Cuts at: 2113 2604 2113
Size: 491 5001
Bme1580I G_kGCm'C
Cuts at: 3117 3210 4266 3117
Size: 93 1056 4343
Fragments arranged by size:
4343 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1071 2704 2785 3120 5264 1071
Size: 1633 81 335 2144 1299
Fragments arranged by size:
2144 1633 1299 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1089 3859 1089
Size: 2770 2722
Bpu10I CC'TnA_GC
Cuts at: 914 1908 914
Size: 994 4498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2668 3547 4039 4337 2668
Size: 879 492 298 3823
Fragments arranged by size:
3823 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5196 5196
Size: 5492
BsaAI yAC'GTr
Cuts at: 2845 2845
Size: 5492
BsaBI GATnn'nnATC
Cuts at: 6 755 3006 5043 6
Size: 749 2251 2037 455
Fragments arranged by size:
2251 2037 749 455
BsaHI Gr'CG_yC
Cuts at: 146 2923 3171 3873 5369 146
Size: 2777 248 702 1496 269
Fragments arranged by size:
2777 1496 702 269 248
BsaWI w'CCGG_w
Cuts at: 2681 3202 3880 4154 4301 4712 2681
Size: 521 678 274 147 411 3461
Fragments arranged by size:
3461 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2127 2157 2127
Size: 30 5462
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 141 144 147 247 811 141
Size: 3 3 100 564 4822
Fragments arranged by size:
4822 564 100 3 3
BseYI C'CCAG_C
Cuts at: 2917 3675 4252 4748 2917
Size: 758 577 496 3661
Fragments arranged by size:
3661 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3034 3080 4288 104
Size: 2930 46 1208 1308
Fragments arranged by size:
2930 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 133 2506 3284 3474 4266 133
Size: 2373 778 190 792 1359
Fragments arranged by size:
2373 1359 792 778 190
BsmI GAATG_Cn'
Cuts at: 694 732 1579 694
Size: 38 847 4607
Fragments arranged by size:
4607 847 38
BsmBI CGTCTCn'nnnn_
Cuts at: 1648 1648
Size: 5492
Bsp1286I G_dGCh'C
Cuts at: 133 2506 3117 3210 3284 3474 3536 4266 133
Size: 2373 611 93 74 190 62 730 1359
Fragments arranged by size:
2373 1359 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4712 4712
Size: 5492
BspHI T'CATG_A
Cuts at: 1490 1548 2070 4668 1490
Size: 58 522 2598 2314
Fragments arranged by size:
2598 2314 522 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1885 2542 2756 3058 3439 1885
Size: 657 214 302 381 3938
Fragments arranged by size:
3938 657 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3784 3838 5322 5487 50
Size: 3734 54 1484 165 55
Fragments arranged by size:
3734 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 904 1854 2042 3404 904
Size: 950 188 1362 2992
Fragments arranged by size:
2992 1362 950 188
BsrFI r'CCGG_y
Cuts at: 3490 3671 3880 3490
Size: 181 209 5102
Fragments arranged by size:
5102 209 181
BssSI C'ACGA_G
Cuts at: 944 3763 4121 944
Size: 2819 358 2315
Fragments arranged by size:
2819 2315 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 2869 1516
Size: 1353 4139
BstBI TT'CG_AA
Cuts at: 3853 3853
Size: 5492
BstEII G'GTnAC_C
Cuts at: 1129 1129
Size: 5492
BstXI CCAn_nnnn'nTGG
Cuts at: 679 2028 679
Size: 1349 4143
BstYI r'GATC_y
Cuts at: 1 801 2406 2517 2984 3007 3342 3588 4600
Size: 800 1605 111 467 23 335 246 1012
Cuts at: 4600 4686 4698 5044 1
Size: 86 12 346 449
Fragments arranged by size:
1605 1012 800 467 449 346 335 246
111 86 23 12
BstZ17I GTA'TAC
Cuts at: 1658 1658
Size: 5492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3442 3623 3786 3442
Size: 181 163 5148
Fragments arranged by size:
5148 181 163
BtsI GCAGTG_nn'
Cuts at: 2735 2735
Size: 5492
ClaI AT'CG_AT
Cuts at: 759 759
Size: 5492
DraI TTT'AAA
Cuts at: 839 2258 2491 839
Size: 1419 233 3840
Fragments arranged by size:
3840 1419 233
DraIII CAC_nnn'GTG
Cuts at: 698 698
Size: 5492
DrdI GACnn_nn'nnGTC
Cuts at: 3198 4056 3198
Size: 858 4634
EaeI y'GGCC_r
Cuts at: 767 1189 3077 3251 3642 3669 5014 767
Size: 422 1888 174 391 27 1345 1245
Fragments arranged by size:
1888 1345 1245 422 391 174 27
EagI C'GGCC_G
Cuts at: 3077 3077
Size: 5492
EarI CTCTTCn'nnn_
Cuts at: 186 2278 3515 3725 186
Size: 2092 1237 210 1953
Fragments arranged by size:
2092 1953 1237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3700 4010 4156 5302 3700
Size: 310 146 1146 3890
Fragments arranged by size:
3890 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2504 2504
Size: 5492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 488 3317 3749 4496 4764 229
Size: 259 2829 432 747 268 957
Fragments arranged by size:
2829 957 747 432 268 259
EcoO109I rG'GnC_Cy
Cuts at: 2631 2631
Size: 5492
EcoRI G'AATT_C
Cuts at: 551 865 939 1494 2496 551
Size: 314 74 555 1002 3547
Fragments arranged by size:
3547 1002 555 314 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 276 3511 276
Size: 3235 2257
FspI TGC'GCA
Cuts at: 3273 3273
Size: 5492
HaeII r_GCGC'y
Cuts at: 149 208 2926 3174 4196 5175 149
Size: 59 2718 248 1022 979 466
Fragments arranged by size:
2718 1022 979 466 248 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2052 2084 2202 2234 3334 3364 3366 3396 2052
Size: 32 118 32 1100 30 2 30 4148
Fragments arranged by size:
4148 1100 118 32 32 30 30 2
HincII GTy'rAC
Cuts at: 791 2531 791
Size: 1740 3752
HindIII A'AGCT_T
Cuts at: 253 2049 253
Size: 1796 3696
HpaI GTT'AAC
Cuts at: 791 791
Size: 5492
KasI G'GCGC_C
Cuts at: 145 2922 3170 145
Size: 2777 248 2467
Fragments arranged by size:
2777 2467 248
KpnI G_GTAC'C
Cuts at: 2512 2512
Size: 5492
MfeI C'AATT_G
Cuts at: 1763 1763
Size: 5492
MluI A'CGCG_T
Cuts at: 4925 4925
Size: 5492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1427 4163 4347 1427
Size: 2736 184 2572
Fragments arranged by size:
2736 2572 184
MscI TGG'CCA
Cuts at: 769 1191 3253 769
Size: 422 2062 3008
Fragments arranged by size:
3008 2062 422
MslI CAynn'nnrTG
Cuts at: 3481 3608 3481
Size: 127 5365
MspA1I CmG'CkG
Cuts at: 2603 2917 3277 4290 4535 2603
Size: 314 360 1013 245 3560
Fragments arranged by size:
3560 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3673 3673
Size: 5492
NarI GG'CG_CC
Cuts at: 146 2923 3171 146
Size: 2777 248 2467
Fragments arranged by size:
2777 2467 248
NgoMIV G'CCGG_C
Cuts at: 3671 3671
Size: 5492
NsiI A_TGCA'T
Cuts at: 1581 1581
Size: 5492
NspI r_CATG'y
Cuts at: 1918 2545 3045 3952 1918
Size: 627 500 907 3458
Fragments arranged by size:
3458 907 627 500
PciI A'CATG_T
Cuts at: 3948 3948
Size: 5492
PmeI GTTT'AAAC
Cuts at: 2491 2491
Size: 5492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 4631
Size: 32 5460
PshAI GACnn'nnGTC
Cuts at: 1122 1122
Size: 5492
PspXI vC'TCGA_Gb
Cuts at: 2096 2096
Size: 5492
PstI C_TGCA'G
Cuts at: 410 441 1961 2539 3224 410
Size: 31 1520 578 685 2678
Fragments arranged by size:
2678 1520 685 578 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5492
PvuII CAG'CTG
Cuts at: 2917 3277 2917
Size: 360 5132
RsrII CG'GwC_CG
Cuts at: 3687 3687
Size: 5492
SacI G_AGCT'C
Cuts at: 2506 2506
Size: 5492
SalI G'TCGA_C
Cuts at: 2529 2529
Size: 5492
SapI GCTCTTCn'nnn_
Cuts at: 3515 3725 3515
Size: 210 5282
SbfI CC_TGCA'GG
Cuts at: 2539 2539
Size: 5492
ScaI AGT'ACT
Cuts at: 5009 5009
Size: 5492
SfcI C'TryA_G
Cuts at: 33 406 437 530 1957 2535 3220 4213 4404
Size: 373 31 93 1427 578 685 993 191
Cuts at: 4404 33
Size: 1121
Fragments arranged by size:
1427 1121 993 685 578 373 191 93
31
SfoI GGC'GCC
Cuts at: 147 2924 3172 147
Size: 2777 248 2467
Fragments arranged by size:
2777 2467 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5492
SmaI CCC'GGG
Cuts at: 2514 2514
Size: 5492
SmlI C'TyrA_G
Cuts at: 2096 2647 3562 4054 4316 2096
Size: 551 915 492 262 3272
Fragments arranged by size:
3272 915 551 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5492
SphI G_CATG'C
Cuts at: 2545 3045 2545
Size: 500 4992
StyI C'CwwG_G
Cuts at: 2626 2980 2626
Size: 354 5138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3075 3208 3667 3876 3075
Size: 133 459 209 4691
Fragments arranged by size:
4691 459 209 133
TatI w'GTAC_w
Cuts at: 2362 5007 2362
Size: 2645 2847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 930 962 5009 5041 930
Size: 32 4047 32 1381
Fragments arranged by size:
4047 1381 32 32
Tth111I GACn'n_nGTC
Cuts at: 3289 3289
Size: 5492
XbaI T'CTAG_A
Cuts at: 2523 2523
Size: 5492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1671 1867 2028 2987 1671
Size: 196 161 959 4176
Fragments arranged by size:
4176 959 196 161
XhoI C'TCGA_G
Cuts at: 2096 2096
Size: 5492
XmaI C'CCGG_G
Cuts at: 2512 2512
Size: 5492
XmnI GAAnn'nnTTC
Cuts at: 256 551 959 256
Size: 295 408 4789
Fragments arranged by size:
4789 408 295
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PmeI PpiI PshAI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI BpmI
BsgI BsiWI BsrGI BssHII Bsu36I BtgI CspCI EcoNI
EcoRV FseI FspAI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI