Restriction map for
pF1KE2671
(Circular) (Six-base) MAPSORT of: pF1KE2671.seq Check: 1906 from: 1 to: 4112
pF1KE2671 4112 bp
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AleI CACnn'nnGTG
Cuts at: 923 923
Size: 4112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1084 1116 1084
Size: 32 4080
AlwNI CAG_nnn'CTG
Cuts at: 565 1417 2984 565
Size: 852 1567 1693
Fragments arranged by size:
1693 1567 852
ApaLI G'TGCA_C
Cuts at: 934 2882 934
Size: 1948 2164
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 185 296 1132 185
Size: 111 836 3165
Fragments arranged by size:
3165 836 111
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 455 485 1128 1542 1790 1825 455
Size: 30 643 414 248 35 2742
Fragments arranged by size:
2742 643 414 248 35 30
BanII G_rGCy'C
Cuts at: 589 1126 2156 589
Size: 537 1030 2545
Fragments arranged by size:
2545 1030 537
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BbsI GAAGACnn'nnnn_
Cuts at: 197 197
Size: 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglI GCCn_nnn'nGGC
Cuts at: 145 562 145
Size: 417 3695
BglII A'GATC_T
Cuts at: 1627 3664 1627
Size: 2037 2075
BlpI GC'TnA_GC
Cuts at: 841 1224 841
Size: 383 3729
Bme1580I G_kGCm'C
Cuts at: 488 938 1737 1830 2886 488
Size: 450 799 93 1056 1714
Fragments arranged by size:
1714 1056 799 450 93
BmgBI CAC'GTC
Cuts at: 582 1100 582
Size: 518 3594
BmrI ACTGGGnnnn_n'
Cuts at: 517 553 1324 1405 1740 3884 517
Size: 36 771 81 335 2144 745
Fragments arranged by size:
2144 771 745 335 81 36
BmtI G_CTAG'C
Cuts at: 412 412
Size: 4112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1093 232
Size: 861 3251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 994 1288 2167 2659 2957 343
Size: 651 294 879 492 298 1498
Fragments arranged by size:
1498 879 651 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 703 3816 703
Size: 3113 999
BsaAI yAC'GTr
Cuts at: 1465 1465
Size: 4112
BsaBI GATnn'nnATC
Cuts at: 6 779 1626 3663 6
Size: 773 847 2037 455
Fragments arranged by size:
2037 847 773 455
BsaHI Gr'CG_yC
Cuts at: 346 962 1543 1791 2493 3989 346
Size: 616 581 248 702 1496 469
Fragments arranged by size:
1496 702 616 581 469 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 571 601 571
Size: 30 4082
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 414 655 388
Size: 26 241 3845
Fragments arranged by size:
3845 241 26
BseYI C'CCAG_C
Cuts at: 1537 2295 2872 3368 1537
Size: 758 577 496 2281
Fragments arranged by size:
2281 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 938 1126 1904 2094 2886 938
Size: 188 778 190 792 2164
Fragments arranged by size:
2164 792 778 190 188
BsmBI CGTCTCn'nnnn_
Cuts at: 610 610
Size: 4112
Bsp1286I G_dGCh'C
Cuts at: 488 589 938 1126 1737 1830 1904 2094 2156
Size: 101 349 188 611 93 74 190 62
Cuts at: 2156 2886 488
Size: 730 1714
Fragments arranged by size:
1714 730 611 349 190 188 101 93
74 62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 695 938 2024 695
Size: 243 1086 2783
Fragments arranged by size:
2783 1086 243
BsrFI r'CCGG_y
Cuts at: 488 943 2110 2291 2500 488
Size: 455 1167 181 209 2100
Fragments arranged by size:
2100 1167 455 209 181
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 492 1489 492
Size: 997 3115
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 909 909
Size: 4112
BstYI r'GATC_y
Cuts at: 1 390 1137 1604 1627 1962 2208 3220 3306
Size: 389 747 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1012 747 467 449 389 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 451 451
Size: 4112
BtgI C'CryG_G
Cuts at: 107 862 887 1016 107
Size: 755 25 129 3203
Fragments arranged by size:
3203 755 129 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 1355 1355
Size: 4112
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 137 899 1055 1697 1871 2262 2289 3634 137
Size: 762 156 642 174 391 27 1345 615
Fragments arranged by size:
1345 762 642 615 391 174 156 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 613 2135 2345 613
Size: 1522 210 2380
Fragments arranged by size:
2380 1522 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1093 1937 2369 3116 3384 232
Size: 861 844 432 747 268 960
Fragments arranged by size:
960 861 844 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1050 1251 1050
Size: 201 3911
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 1546 1794 2816 3795 1546
Size: 248 1022 979 1863
Fragments arranged by size:
1863 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 375 407 503 535 571 603 1954 1984 1986
Size: 32 96 32 36 32 1351 30 2
Cuts at: 1986 2016 375
Size: 30 2471
Fragments arranged by size:
2471 1351 96 36 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 139 901 1057 1873 139
Size: 762 156 816 2378
Fragments arranged by size:
2378 816 762 156
MslI CAynn'nnrTG
Cuts at: 923 992 2101 2228 923
Size: 69 1109 127 2807
Fragments arranged by size:
2807 1109 127 69
MspA1I CmG'CkG
Cuts at: 125 464 829 1223 1537 1897 2910 3155 125
Size: 339 365 394 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 394 365 360 339 314 245
NaeI GCC'GGC
Cuts at: 945 2293 945
Size: 1348 2764
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NcoI C'CATG_G
Cuts at: 107 1016 107
Size: 909 3203
NgoMIV G'CCGG_C
Cuts at: 943 2291 943
Size: 1348 2764
NheI G'CTAG_C
Cuts at: 408 408
Size: 4112
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PflMI CCAn_nnn'nTGG
Cuts at: 565 565
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 829 1537 1897 829
Size: 708 360 3044
Fragments arranged by size:
3044 708 360
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 1155 1840 2833 3024 33
Size: 1122 685 993 191 1121
Fragments arranged by size:
1122 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 187 298 1134 187
Size: 111 836 3165
Fragments arranged by size:
3165 836 111
SmlI C'TyrA_G
Cuts at: 322 1009 1267 2182 2674 2936 322
Size: 687 258 915 492 262 1498
Fragments arranged by size:
1498 915 687 492 262 258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StuI AGG'CCT
Cuts at: 271 271
Size: 4112
StyI C'CwwG_G
Cuts at: 107 377 1016 1246 1600 107
Size: 270 639 230 354 2619
Fragments arranged by size:
2619 639 354 270 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1695 1828 2287 2496 1695
Size: 133 459 209 3311
Fragments arranged by size:
3311 459 209 133
TatI w'GTAC_w
Cuts at: 3627 3627
Size: 4112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 653 685 3629 3661 653
Size: 32 2944 32 1104
Fragments arranged by size:
2944 1104 32 32
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 147 894 1607 147
Size: 747 713 2652
Fragments arranged by size:
2652 747 713
XmaI C'CCGG_G
Cuts at: 185 296 1132 185
Size: 111 836 3165
Fragments arranged by size:
3165 836 111
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI Bpu10I BsgI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI