Restriction map for
pF1KE2666
(Circular) (Six-base) MAPSORT of: pF1KE2666.seq Check: 9224 from: 1 to: 3878
pF1KE2666 3878 bp
With 182 enzymes: SgfI *
January 12, 2017 11:32 ..
AccI GT'mk_AC
Cuts at: 916 916
Size: 3878
Acc65I G'GTAC_C
Cuts at: 894 894
Size: 3878
AclI AA'CG_TT
Cuts at: 377 3718 377
Size: 3341 537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 1703 2135 2882 3150 464
Size: 1239 432 747 268 1192
Fragments arranged by size:
1239 1192 747 432 268
AflIII A'CryG_T
Cuts at: 794 1230 2334 3311 794
Size: 436 1104 977 1361
Fragments arranged by size:
1361 1104 977 436
AgeI A'CCGG_T
Cuts at: 2266 2266
Size: 3878
AlwNI CAG_nnn'CTG
Cuts at: 1183 2750 1183
Size: 1567 2311
ApaLI G'TGCA_C
Cuts at: 2648 2648
Size: 3878
ApoI r'AATT_y
Cuts at: 284 666 882 3231 3487 284
Size: 382 216 2349 256 675
Fragments arranged by size:
2349 675 382 256 216
AseI AT'TA_AT
Cuts at: 20 590 20
Size: 570 3308
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
AvaI C'yCGr_G
Cuts at: 898 898
Size: 3878
BamHI G'GATC_C
Cuts at: 903 903
Size: 3878
BanI G'GyrC_C
Cuts at: 894 1308 1556 1591 894
Size: 414 248 35 3181
Fragments arranged by size:
3181 414 248 35
BanII G_rGCy'C
Cuts at: 892 1922 892
Size: 1030 2848
BbeI G_GCGC'C
Cuts at: 1312 1560 1312
Size: 248 3630
BciVI GTATCCnnnnn_n'
Cuts at: 711 1776 2537 3878 711
Size: 1065 761 1341 711
Fragments arranged by size:
1341 1065 761 711
BclI T'GATC_A
Cuts at: 1398 1398
Size: 3878
BglII A'GATC_T
Cuts at: 120 432 1393 3430 120
Size: 312 961 2037 568
Fragments arranged by size:
2037 961 568 312
BlpI GC'TnA_GC
Cuts at: 990 990
Size: 3878
Bme1580I G_kGCm'C
Cuts at: 1503 1596 2652 1503
Size: 93 1056 2729
Fragments arranged by size:
2729 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1090 1171 1506 3650 1090
Size: 81 335 2144 1318
Fragments arranged by size:
2144 1318 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 3878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 380 1054 1933 2425 2723 301
Size: 79 674 879 492 298 1456
Fragments arranged by size:
1456 879 674 492 298 79
BsaI GGTCTCn'nnnn_
Cuts at: 3582 3582
Size: 3878
BsaAI yAC'GTr
Cuts at: 795 1231 795
Size: 436 3442
BsaBI GATnn'nnATC
Cuts at: 6 437 1392 3429 6
Size: 431 955 2037 455
Fragments arranged by size:
2037 955 455 431
BsaHI Gr'CG_yC
Cuts at: 1309 1557 2259 3755 1309
Size: 248 702 1496 1432
Fragments arranged by size:
1496 1432 702 248
BsaWI w'CCGG_w
Cuts at: 1067 1588 2266 2540 2687 3098 1067
Size: 521 678 274 147 411 1847
Fragments arranged by size:
1847 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1303 2061 2638 3134 1303
Size: 758 577 496 2047
Fragments arranged by size:
2047 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1420 1466 2674 104
Size: 1316 46 1208 1308
Fragments arranged by size:
1316 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 892 1670 1860 2652 892
Size: 778 190 792 2118
Fragments arranged by size:
2118 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 892 1503 1596 1670 1860 1922 2652 892
Size: 611 93 74 190 62 730 2118
Fragments arranged by size:
2118 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3098 3098
Size: 3878
BspHI T'CATG_A
Cuts at: 3054 3054
Size: 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 928 1142 1444 1825 928
Size: 214 302 381 2981
Fragments arranged by size:
2981 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2170 2224 3708 3873 50
Size: 2120 54 1484 165 55
Fragments arranged by size:
2120 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1790 1790
Size: 3878
BsrFI r'CCGG_y
Cuts at: 1876 2057 2266 1876
Size: 181 209 3488
Fragments arranged by size:
3488 209 181
BssSI C'ACGA_G
Cuts at: 2149 2507 2149
Size: 358 3520
BstAPI GCAn_nnn'nTGC
Cuts at: 1255 1255
Size: 3878
BstBI TT'CG_AA
Cuts at: 2239 2239
Size: 3878
BstYI r'GATC_y
Cuts at: 1 120 432 903 1370 1393 1728 1974 2986
Size: 119 312 471 467 23 335 246 1012
Cuts at: 2986 3072 3084 3430 1
Size: 86 12 346 449
Fragments arranged by size:
1012 471 467 449 346 335 312 246
119 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3878
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1828 2009 2172 1828
Size: 181 163 3534
Fragments arranged by size:
3534 181 163
BtsI GCAGTG_nn'
Cuts at: 816 1121 816
Size: 305 3573
DraI TTT'AAA
Cuts at: 877 877
Size: 3878
DrdI GACnn_nn'nnGTC
Cuts at: 1584 2442 1584
Size: 858 3020
EaeI y'GGCC_r
Cuts at: 195 1463 1637 2028 2055 3400 195
Size: 1268 174 391 27 1345 673
Fragments arranged by size:
1345 1268 673 391 174 27
EagI C'GGCC_G
Cuts at: 1463 1463
Size: 3878
EarI CTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2086 2396 2542 3688 2086
Size: 310 146 1146 2276
Fragments arranged by size:
2276 1146 310 146
EcoICRI GAG'CTC
Cuts at: 890 890
Size: 3878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 1703 2135 2882 3150 464
Size: 1239 432 747 268 1192
Fragments arranged by size:
1239 1192 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1017 1017
Size: 3878
EcoRI G'AATT_C
Cuts at: 666 882 666
Size: 216 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 192 1897 192
Size: 1705 2173
FspI TGC'GCA
Cuts at: 1659 1659
Size: 3878
HaeII r_GCGC'y
Cuts at: 1312 1560 2582 3561 1312
Size: 248 1022 979 1629
Fragments arranged by size:
1629 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 666 698 1720 1750 1752 1782 227
Size: 32 407 32 1022 30 2 30 2323
Fragments arranged by size:
2323 1022 407 32 32 30 30 2
HincII GTy'rAC
Cuts at: 917 917
Size: 3878
KasI G'GCGC_C
Cuts at: 1308 1556 1308
Size: 248 3630
KpnI G_GTAC'C
Cuts at: 898 898
Size: 3878
MluI A'CGCG_T
Cuts at: 3311 3311
Size: 3878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2549 2733 2549
Size: 184 3694
MscI TGG'CCA
Cuts at: 197 1639 197
Size: 1442 2436
MslI CAynn'nnrTG
Cuts at: 146 1867 1994 146
Size: 1721 127 2030
Fragments arranged by size:
2030 1721 127
MspA1I CmG'CkG
Cuts at: 989 1303 1663 2676 2921 989
Size: 314 360 1013 245 1946
Fragments arranged by size:
1946 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 3878
NarI GG'CG_CC
Cuts at: 1309 1557 1309
Size: 248 3630
NcoI C'CATG_G
Cuts at: 107 107
Size: 3878
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 3878
NspI r_CATG'y
Cuts at: 931 1431 2338 931
Size: 500 907 2471
Fragments arranged by size:
2471 907 500
PciI A'CATG_T
Cuts at: 2334 2334
Size: 3878
PmeI GTTT'AAAC
Cuts at: 877 877
Size: 3878
PmlI CAC'GTG
Cuts at: 795 795
Size: 3878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3846
PsiI TTA'TAA
Cuts at: 526 868 526
Size: 342 3536
PstI C_TGCA'G
Cuts at: 925 1610 925
Size: 685 3193
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3878
PvuII CAG'CTG
Cuts at: 1303 1663 1303
Size: 360 3518
RsrII CG'GwC_CG
Cuts at: 2073 2073
Size: 3878
SacI G_AGCT'C
Cuts at: 892 892
Size: 3878
SalI G'TCGA_C
Cuts at: 915 915
Size: 3878
SapI GCTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
SbfI CC_TGCA'GG
Cuts at: 925 925
Size: 3878
ScaI AGT'ACT
Cuts at: 3395 3395
Size: 3878
SfcI C'TryA_G
Cuts at: 33 921 1606 2599 2790 33
Size: 888 685 993 191 1121
Fragments arranged by size:
1121 993 888 685 191
SfoI GGC'GCC
Cuts at: 1310 1558 1310
Size: 248 3630
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
SmaI CCC'GGG
Cuts at: 900 900
Size: 3878
SmlI C'TyrA_G
Cuts at: 316 395 1033 1948 2440 2702 316
Size: 79 638 915 492 262 1492
Fragments arranged by size:
1492 915 638 492 262 79
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3878
SphI G_CATG'C
Cuts at: 931 1431 931
Size: 500 3378
SspI AAT'ATT
Cuts at: 165 807 165
Size: 642 3236
StyI C'CwwG_G
Cuts at: 107 1012 1366 107
Size: 905 354 2619
Fragments arranged by size:
2619 905 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1461 1594 2053 2262 1461
Size: 133 459 209 3077
Fragments arranged by size:
3077 459 209 133
TatI w'GTAC_w
Cuts at: 3393 3393
Size: 3878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 3846
Tth111I GACn'n_nGTC
Cuts at: 1675 1675
Size: 3878
XbaI T'CTAG_A
Cuts at: 909 909
Size: 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1373 1373
Size: 3878
XmaI C'CCGG_G
Cuts at: 898 898
Size: 3878
XmnI GAAnn'nnTTC
Cuts at: 377 377
Size: 3878
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PmlI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI