Restriction map for
pF1KE2662
(Circular) (Six-base) MAPSORT of: pF1KE2662.seq Check: 9261 from: 1 to: 3854
pF1KE2662 3854 bp
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 343 448 343
Size: 105 3749
AccI GT'mk_AC
Cuts at: 479 892 479
Size: 413 3441
Acc65I G'GTAC_C
Cuts at: 870 870
Size: 3854
AclI AA'CG_TT
Cuts at: 3694 3694
Size: 3854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 1679 2111 2858 3126 342
Size: 1337 432 747 268 1070
Fragments arranged by size:
1337 1070 747 432 268
AflIII A'CryG_T
Cuts at: 578 1206 2310 3287 578
Size: 628 1104 977 1145
Fragments arranged by size:
1145 1104 977 628
AgeI A'CCGG_T
Cuts at: 2242 2242
Size: 3854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 534 534
Size: 3854
AlwNI CAG_nnn'CTG
Cuts at: 1159 2726 1159
Size: 1567 2287
ApaLI G'TGCA_C
Cuts at: 2624 2624
Size: 3854
ApoI r'AATT_y
Cuts at: 858 3207 3463 858
Size: 2349 256 1249
Fragments arranged by size:
2349 1249 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
AvaI C'yCGr_G
Cuts at: 787 874 787
Size: 87 3767
BamHI G'GATC_C
Cuts at: 879 879
Size: 3854
BanI G'GyrC_C
Cuts at: 870 1284 1532 1567 870
Size: 414 248 35 3157
Fragments arranged by size:
3157 414 248 35
BanII G_rGCy'C
Cuts at: 332 346 734 763 868 1898 332
Size: 14 388 29 105 1030 2288
Fragments arranged by size:
2288 1030 388 105 29 14
BbeI G_GCGC'C
Cuts at: 1288 1536 1288
Size: 248 3606
BbsI GAAGACnn'nnnn_
Cuts at: 219 219
Size: 3854
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3820
BciVI GTATCCnnnnn_n'
Cuts at: 1752 2513 3854 1752
Size: 761 1341 1752
Fragments arranged by size:
1752 1341 761
BclI T'GATC_A
Cuts at: 1374 1374
Size: 3854
BglI GCCn_nnn'nGGC
Cuts at: 385 385
Size: 3854
BglII A'GATC_T
Cuts at: 485 1369 3406 485
Size: 884 2037 933
Fragments arranged by size:
2037 933 884
BlpI GC'TnA_GC
Cuts at: 966 966
Size: 3854
Bme1580I G_kGCm'C
Cuts at: 1479 1572 2628 1479
Size: 93 1056 2705
Fragments arranged by size:
2705 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 252 1066 1147 1482 3626 252
Size: 814 81 335 2144 480
Fragments arranged by size:
2144 814 480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 3854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1030 1909 2401 2699 718
Size: 312 879 492 298 1873
Fragments arranged by size:
1873 879 492 312 298
BsaI GGTCTCn'nnnn_
Cuts at: 559 3558 559
Size: 2999 855
BsaAI yAC'GTr
Cuts at: 1207 1207
Size: 3854
BsaBI GATnn'nnATC
Cuts at: 6 1368 3405 6
Size: 1362 2037 455
Fragments arranged by size:
2037 1362 455
BsaHI Gr'CG_yC
Cuts at: 1285 1533 2235 3731 1285
Size: 248 702 1496 1408
Fragments arranged by size:
1496 1408 702 248
BsaWI w'CCGG_w
Cuts at: 1043 1564 2242 2516 2663 3074 1043
Size: 521 678 274 147 411 1823
Fragments arranged by size:
1823 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 742 742
Size: 3854
BseYI C'CCAG_C
Cuts at: 554 629 696 1279 2037 2614 3110 554
Size: 75 67 583 758 577 496 1298
Fragments arranged by size:
1298 758 583 577 496 75 67
BsiEI CG_ry'CG
Cuts at: 104 392 1396 1442 2650 104
Size: 288 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 288 46
BsiHKAI G_wGCw'C
Cuts at: 149 265 332 734 868 1646 1836 2628 149
Size: 116 67 402 134 778 190 792 1375
Fragments arranged by size:
1375 792 778 402 190 134 116 67
Bsp1286I G_dGCh'C
Cuts at: 149 265 332 346 734 763 868 1479 1572
Size: 116 67 14 388 29 105 611 93
Cuts at: 1572 1646 1836 1898 2628 149
Size: 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 388 190 116 105 93
74 67 62 29 14
BspEI T'CCGG_A
Cuts at: 3074 3074
Size: 3854
BspHI T'CATG_A
Cuts at: 3030 3030
Size: 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 343 448 725 904 1118 1420 1801 343
Size: 105 277 179 214 302 381 2396
Fragments arranged by size:
2396 381 302 277 214 179 105
BsrBI CCG'CTC
Cuts at: 50 2146 2200 3684 3849 50
Size: 2096 54 1484 165 55
Fragments arranged by size:
2096 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1766 1766
Size: 3854
BsrFI r'CCGG_y
Cuts at: 704 1852 2033 2242 704
Size: 1148 181 209 2316
Fragments arranged by size:
2316 1148 209 181
BssSI C'ACGA_G
Cuts at: 142 2125 2483 142
Size: 1983 358 1513
Fragments arranged by size:
1983 1513 358
BstAPI GCAn_nnn'nTGC
Cuts at: 153 440 1231 153
Size: 287 791 2776
Fragments arranged by size:
2776 791 287
BstBI TT'CG_AA
Cuts at: 2215 2215
Size: 3854
BstYI r'GATC_y
Cuts at: 1 485 879 1346 1369 1704 1950 2962 3048
Size: 484 394 467 23 335 246 1012 86
Cuts at: 3048 3060 3406 1
Size: 12 346 449
Fragments arranged by size:
1012 484 467 449 394 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 413 827 413
Size: 414 3440
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1804 1985 2148 1804
Size: 181 163 3510
Fragments arranged by size:
3510 181 163
BtsI GCAGTG_nn'
Cuts at: 1097 1097
Size: 3854
DraI TTT'AAA
Cuts at: 853 853
Size: 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1560 2418 1560
Size: 858 2996
EaeI y'GGCC_r
Cuts at: 389 644 1439 1613 2004 2031 3376 389
Size: 255 795 174 391 27 1345 867
Fragments arranged by size:
1345 867 795 391 255 174 27
EagI C'GGCC_G
Cuts at: 389 1439 389
Size: 1050 2804
EarI CTCTTCn'nnn_
Cuts at: 295 1877 2087 295
Size: 1582 210 2062
Fragments arranged by size:
2062 1582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2062 2372 2518 3664 2062
Size: 310 146 1146 2252
Fragments arranged by size:
2252 1146 310 146
EcoICRI GAG'CTC
Cuts at: 330 732 866 330
Size: 402 134 3318
Fragments arranged by size:
3318 402 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 1679 2111 2858 3126 342
Size: 1337 432 747 268 1070
Fragments arranged by size:
1337 1070 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 721 721
Size: 3854
EcoO109I rG'GnC_Cy
Cuts at: 844 993 844
Size: 149 3705
EcoRI G'AATT_C
Cuts at: 858 858
Size: 3854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3854
FspI TGC'GCA
Cuts at: 1635 1635
Size: 3854
HaeII r_GCGC'y
Cuts at: 380 1288 1536 2558 3537 380
Size: 908 248 1022 979 697
Fragments arranged by size:
1022 979 908 697 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 797 829 1696 1726 1728 1758 797
Size: 32 867 30 2 30 2893
Fragments arranged by size:
2893 867 32 30 30 2
HincII GTy'rAC
Cuts at: 893 893
Size: 3854
KasI G'GCGC_C
Cuts at: 1284 1532 1284
Size: 248 3606
KpnI G_GTAC'C
Cuts at: 874 874
Size: 3854
MluI A'CGCG_T
Cuts at: 3287 3287
Size: 3854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2709 2525
Size: 184 3670
MscI TGG'CCA
Cuts at: 646 1615 646
Size: 969 2885
MslI CAynn'nnrTG
Cuts at: 175 217 1843 1970 175
Size: 42 1626 127 2059
Fragments arranged by size:
2059 1626 127 42
MspA1I CmG'CkG
Cuts at: 311 965 1279 1639 2652 2897 311
Size: 654 314 360 1013 245 1268
Fragments arranged by size:
1268 1013 654 360 314 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 3854
NarI GG'CG_CC
Cuts at: 1285 1533 1285
Size: 248 3606
NcoI C'CATG_G
Cuts at: 413 413
Size: 3854
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 3854
NotI GC'GGCC_GC
Cuts at: 389 389
Size: 3854
NspI r_CATG'y
Cuts at: 156 582 907 1407 2314 156
Size: 426 325 500 907 1696
Fragments arranged by size:
1696 907 500 426 325
PasI CC'CwG_GG
Cuts at: 609 609
Size: 3854
PciI A'CATG_T
Cuts at: 578 2310 578
Size: 1732 2122
PfoI T'CCnGG_A
Cuts at: 572 572
Size: 3854
PmeI GTTT'AAAC
Cuts at: 853 853
Size: 3854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 3822
PpuMI rG'GwC_Cy
Cuts at: 844 844
Size: 3854
PstI C_TGCA'G
Cuts at: 725 901 1586 725
Size: 176 685 2993
Fragments arranged by size:
2993 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3854
PvuII CAG'CTG
Cuts at: 1279 1639 1279
Size: 360 3494
RsrII CG'GwC_CG
Cuts at: 2049 2049
Size: 3854
SacI G_AGCT'C
Cuts at: 332 734 868 332
Size: 402 134 3318
Fragments arranged by size:
3318 402 134
SalI G'TCGA_C
Cuts at: 891 891
Size: 3854
SanDI GG'GwC_CC
Cuts at: 844 844
Size: 3854
SapI GCTCTTCn'nnn_
Cuts at: 1877 2087 1877
Size: 210 3644
SbfI CC_TGCA'GG
Cuts at: 901 901
Size: 3854
ScaI AGT'ACT
Cuts at: 604 3371 604
Size: 2767 1087
SfcI C'TryA_G
Cuts at: 33 721 764 897 1582 2575 2766 33
Size: 688 43 133 685 993 191 1121
Fragments arranged by size:
1121 993 688 685 191 133 43
SfoI GGC'GCC
Cuts at: 1286 1534 1286
Size: 248 3606
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
SmaI CCC'GGG
Cuts at: 789 876 789
Size: 87 3767
SmlI C'TyrA_G
Cuts at: 733 1009 1924 2416 2678 733
Size: 276 915 492 262 1909
Fragments arranged by size:
1909 915 492 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3854
SphI G_CATG'C
Cuts at: 907 1407 907
Size: 500 3354
StuI AGG'CCT
Cuts at: 127 127
Size: 3854
StyI C'CwwG_G
Cuts at: 413 988 1342 413
Size: 575 354 2925
Fragments arranged by size:
2925 575 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 234 1437 1570 2029 2238 234
Size: 1203 133 459 209 1850
Fragments arranged by size:
1850 1203 459 209 133
TatI w'GTAC_w
Cuts at: 602 3369 602
Size: 2767 1087
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 3822
Tth111I GACn'n_nGTC
Cuts at: 1651 1651
Size: 3854
XbaI T'CTAG_A
Cuts at: 885 885
Size: 3854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 1349 216
Size: 1133 2721
XmaI C'CCGG_G
Cuts at: 787 874 787
Size: 87 3767
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI