Restriction map for
pF1KE2660
(Circular) (Six-base) MAPSORT of: pF1KE2660.seq Check: 7517 from: 1 to: 5507
pF1KE2660 5507 bp
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AatII G_ACGT'C
Cuts at: 680 680
Size: 5507
AccI GT'mk_AC
Cuts at: 890 2545 890
Size: 1655 3852
Acc65I G'GTAC_C
Cuts at: 1535 2523 1535
Size: 988 4519
AclI AA'CG_TT
Cuts at: 5347 5347
Size: 5507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 1886 2197 3332 3764 4511 4779 200
Size: 1686 311 1135 432 747 268 928
Fragments arranged by size:
1686 1135 928 747 432 311 268
AflII C'TTAA_G
Cuts at: 2010 2340 2010
Size: 330 5177
AflIII A'CryG_T
Cuts at: 982 1133 1904 2859 3963 4940 982
Size: 151 771 955 1104 977 1549
Fragments arranged by size:
1549 1104 977 955 771 151
AgeI A'CCGG_T
Cuts at: 3895 3895
Size: 5507
AhdI GACnn_n'nnGTC
Cuts at: 134 134
Size: 5507
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 559 591 559
Size: 32 5475
AlwNI CAG_nnn'CTG
Cuts at: 696 2812 4379 696
Size: 2116 1567 1824
Fragments arranged by size:
2116 1824 1567
ApaI G_GGCC'C
Cuts at: 606 606
Size: 5507
ApaLI G'TGCA_C
Cuts at: 4277 4277
Size: 5507
ApoI r'AATT_y
Cuts at: 210 311 868 2511 4860 5116 210
Size: 101 557 1643 2349 256 601
Fragments arranged by size:
2349 1643 601 557 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 5507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
AvaI C'yCGr_G
Cuts at: 2527 2527
Size: 5507
AvrII C'CTAG_G
Cuts at: 1447 1447
Size: 5507
BamHI G'GATC_C
Cuts at: 2167 2532 2167
Size: 365 5142
BanI G'GyrC_C
Cuts at: 454 1535 2062 2523 2937 3185 3220 454
Size: 1081 527 461 414 248 35 2741
Fragments arranged by size:
2741 1081 527 461 414 248 35
BanII G_rGCy'C
Cuts at: 606 1804 2521 3551 606
Size: 1198 717 1030 2562
Fragments arranged by size:
2562 1198 1030 717
BbeI G_GCGC'C
Cuts at: 2941 3189 2941
Size: 248 5259
BbsI GAAGACnn'nnnn_
Cuts at: 881 1017 881
Size: 136 5371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 432 466 432
Size: 34 5473
BciVI GTATCCnnnnn_n'
Cuts at: 329 1787 1832 3405 4166 5507 329
Size: 1458 45 1573 761 1341 329
Fragments arranged by size:
1573 1458 1341 761 329 45
BclI T'GATC_A
Cuts at: 608 1680 3027 608
Size: 1072 1347 3088
Fragments arranged by size:
3088 1347 1072
BfrBI ATG'CAT
Cuts at: 229 864 229
Size: 635 4872
BglI GCCn_nnn'nGGC
Cuts at: 552 552
Size: 5507
BglII A'GATC_T
Cuts at: 3022 5059 3022
Size: 2037 3470
BlpI GC'TnA_GC
Cuts at: 1180 2619 1180
Size: 1439 4068
Bme1580I G_kGCm'C
Cuts at: 606 912 1640 3132 3225 4281 606
Size: 306 728 1492 93 1056 1832
Fragments arranged by size:
1832 1492 1056 728 306 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 1263 1799 2719 2800 3135 5279 724
Size: 539 536 920 81 335 2144 952
Fragments arranged by size:
2144 952 920 539 536 335 81
BmtI G_CTAG'C
Cuts at: 745 745
Size: 5507
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3874 3874
Size: 5507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 547 1314 1662 2408 471
Size: 76 767 348 746 3570
Fragments arranged by size:
3570 767 746 348 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 2683 3562 4054 4352 190
Size: 2493 879 492 298 1345
Fragments arranged by size:
2493 1345 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1189 5211 1189
Size: 4022 1485
BsaAI yAC'GTr
Cuts at: 1122 2860 1122
Size: 1738 3769
BsaBI GATnn'nnATC
Cuts at: 6 1207 1990 2172 3021 5058 6
Size: 1201 783 182 849 2037 455
Fragments arranged by size:
2037 1201 849 783 455 182
BsaHI Gr'CG_yC
Cuts at: 677 2938 3186 3888 5384 677
Size: 2261 248 702 1496 800
Fragments arranged by size:
2261 1496 800 702 248
BsaWI w'CCGG_w
Cuts at: 474 2696 3217 3895 4169 4316 4727 474
Size: 2222 521 678 274 147 411 1254
Fragments arranged by size:
2222 1254 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 559 589 2368 2398 559
Size: 30 1779 30 3668
Fragments arranged by size:
3668 1779 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 1412 2389 403
Size: 1009 977 3521
Fragments arranged by size:
3521 1009 977
BseYI C'CCAG_C
Cuts at: 2134 2932 3690 4267 4763 2134
Size: 798 758 577 496 2878
Fragments arranged by size:
2878 798 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2431 3049 3095 4303 104
Size: 2327 618 46 1208 1308
Fragments arranged by size:
2327 1308 1208 618 46
BsiHKAI G_wGCw'C
Cuts at: 1804 2521 3299 3489 4281 1804
Size: 717 778 190 792 3030
Fragments arranged by size:
3030 792 778 717 190
BsmI GAATG_Cn'
Cuts at: 2272 2272
Size: 5507
Bsp1286I G_dGCh'C
Cuts at: 606 912 1640 1804 2521 3132 3225 3299 3489
Size: 306 728 164 717 611 93 74 190
Cuts at: 3489 3551 4281 606
Size: 62 730 1832
Fragments arranged by size:
1832 730 728 717 611 306 190 164
93 74 62
BspEI T'CCGG_A
Cuts at: 4727 4727
Size: 5507
BspHI T'CATG_A
Cuts at: 1169 4683 1169
Size: 3514 1993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 704 2557 2771 3073 3454 692
Size: 12 1853 214 302 381 2745
Fragments arranged by size:
2745 1853 381 302 214 12
BsrBI CCG'CTC
Cuts at: 50 3799 3853 5337 5502 50
Size: 3749 54 1484 165 55
Fragments arranged by size:
3749 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3419 3419
Size: 5507
BsrFI r'CCGG_y
Cuts at: 2055 3505 3686 3895 2055
Size: 1450 181 209 3667
Fragments arranged by size:
3667 1450 209 181
BsrGI T'GTAC_A
Cuts at: 1745 1745
Size: 5507
BssHII G'CGCG_C
Cuts at: 760 760
Size: 5507
BssSI C'ACGA_G
Cuts at: 1243 3778 4136 1243
Size: 2535 358 2614
Fragments arranged by size:
2614 2535 358
BstAPI GCAn_nnn'nTGC
Cuts at: 352 696 2884 352
Size: 344 2188 2975
Fragments arranged by size:
2975 2188 344
BstBI TT'CG_AA
Cuts at: 3868 3868
Size: 5507
BstXI CCAn_nnnn'nTGG
Cuts at: 525 1779 525
Size: 1254 4253
BstYI r'GATC_y
Cuts at: 1 2167 2532 2999 3022 3357 3603 4615 4701
Size: 2166 365 467 23 335 246 1012 86
Cuts at: 4701 4713 5059 1
Size: 12 346 449
Fragments arranged by size:
2166 1012 467 449 365 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 722 2448 107
Size: 615 1726 3166
Fragments arranged by size:
3166 1726 615
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3457 3638 3801 3457
Size: 181 163 5163
Fragments arranged by size:
5163 181 163
BtsI GCAGTG_nn'
Cuts at: 215 1466 2163 2372 2750 215
Size: 1251 697 209 378 2972
Fragments arranged by size:
2972 1251 697 378 209
DraI TTT'AAA
Cuts at: 2506 2506
Size: 5507
DrdI GACnn_nn'nnGTC
Cuts at: 3213 4071 3213
Size: 858 4649
EaeI y'GGCC_r
Cuts at: 734 3092 3266 3657 3684 5029 734
Size: 2358 174 391 27 1345 1212
Fragments arranged by size:
2358 1345 1212 391 174 27
EagI C'GGCC_G
Cuts at: 3092 3092
Size: 5507
EarI CTCTTCn'nnn_
Cuts at: 1324 1574 2112 2242 2303 3530 3740 1324
Size: 250 538 130 61 1227 210 3091
Fragments arranged by size:
3091 1227 538 250 210 130 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 708 1984 3715 4025 4171 5317 708
Size: 1276 1731 310 146 1146 898
Fragments arranged by size:
1731 1276 1146 898 310 146
EcoICRI GAG'CTC
Cuts at: 1802 2519 1802
Size: 717 4790
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 471 547 1314 1662 1886 2197 2408 3332
Size: 271 76 767 348 224 311 211 924
Cuts at: 3332 3764 4511 4779 200
Size: 432 747 268 928
Fragments arranged by size:
928 924 767 747 432 348 311 271
268 224 211 76
EcoO109I rG'GnC_Cy
Cuts at: 602 603 801 1925 2474 2646 602
Size: 1 198 1124 549 172 3463
Fragments arranged by size:
3463 1124 549 198 172 1
EcoRI G'AATT_C
Cuts at: 2511 2511
Size: 5507
EcoRV GAT'ATC
Cuts at: 278 278
Size: 5507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 5507
FspI TGC'GCA
Cuts at: 1101 3288 1101
Size: 2187 3320
HaeII r_GCGC'y
Cuts at: 433 2941 3189 4211 5190 433
Size: 2508 248 1022 979 750
Fragments arranged by size:
2508 1022 979 750 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 451 483 1861 1893 2016 2048 2152
Size: 32 299 32 1378 32 123 32 104
Cuts at: 2152 2184 2368 2400 2413 2445 3349 3379 3381
Size: 32 184 32 13 32 904 30 2
Cuts at: 3381 3411 120
Size: 30 2216
Fragments arranged by size:
2216 1378 904 299 184 123 104 32
32 32 32 32 32 32 30 30
13 2
HincII GTy'rAC
Cuts at: 681 891 2546 681
Size: 210 1655 3642
Fragments arranged by size:
3642 1655 210
KasI G'GCGC_C
Cuts at: 2937 3185 2937
Size: 248 5259
KpnI G_GTAC'C
Cuts at: 1539 2527 1539
Size: 988 4519
MfeI C'AATT_G
Cuts at: 1007 1007
Size: 5507
MluI A'CGCG_T
Cuts at: 4940 4940
Size: 5507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1925 4178 4362 1925
Size: 2253 184 3070
Fragments arranged by size:
3070 2253 184
MscI TGG'CCA
Cuts at: 3268 3268
Size: 5507
MslI CAynn'nnrTG
Cuts at: 421 823 922 1174 1213 1219 1777 3496 3623
Size: 402 99 252 39 6 558 1719 127
Cuts at: 3623 421
Size: 2305
Fragments arranged by size:
2305 1719 558 402 252 127 99 39
6
MspA1I CmG'CkG
Cuts at: 2443 2618 2932 3292 4305 4550 2443
Size: 175 314 360 1013 245 3400
Fragments arranged by size:
3400 1013 360 314 245 175
NaeI GCC'GGC
Cuts at: 2057 3688 2057
Size: 1631 3876
NarI GG'CG_CC
Cuts at: 2938 3186 2938
Size: 248 5259
NcoI C'CATG_G
Cuts at: 107 2448 107
Size: 2341 3166
NgoMIV G'CCGG_C
Cuts at: 2055 3686 2055
Size: 1631 3876
NheI G'CTAG_C
Cuts at: 741 741
Size: 5507
NsiI A_TGCA'T
Cuts at: 231 866 231
Size: 635 4872
NspI r_CATG'y
Cuts at: 1908 2195 2560 3060 3967 1908
Size: 287 365 500 907 3448
Fragments arranged by size:
3448 907 500 365 287
PasI CC'CwG_GG
Cuts at: 2045 2045
Size: 5507
PciI A'CATG_T
Cuts at: 1904 3963 1904
Size: 2059 3448
PflMI CCAn_nnn'nTGG
Cuts at: 584 584
Size: 5507
PfoI T'CCnGG_A
Cuts at: 2163 2163
Size: 5507
PmeI GTTT'AAAC
Cuts at: 2506 2506
Size: 5507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 559 591 4646 4678 559
Size: 32 4055 32 1388
Fragments arranged by size:
4055 1388 32 32
PpuMI rG'GwC_Cy
Cuts at: 1925 2474 1925
Size: 549 4958
PspOMI G'GGCC_C
Cuts at: 602 602
Size: 5507
PstI C_TGCA'G
Cuts at: 119 692 2115 2554 3239 119
Size: 573 1423 439 685 2387
Fragments arranged by size:
2387 1423 685 573 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5507
PvuII CAG'CTG
Cuts at: 2443 2932 3292 2443
Size: 489 360 4658
Fragments arranged by size:
4658 489 360
RsrII CG'GwC_CG
Cuts at: 3702 3702
Size: 5507
SacI G_AGCT'C
Cuts at: 1804 2521 1804
Size: 717 4790
SalI G'TCGA_C
Cuts at: 889 2544 889
Size: 1655 3852
SapI GCTCTTCn'nnn_
Cuts at: 1574 2242 3530 3740 1574
Size: 668 1288 210 3341
Fragments arranged by size:
3341 1288 668 210
SbfI CC_TGCA'GG
Cuts at: 2554 2554
Size: 5507
ScaI AGT'ACT
Cuts at: 1165 2314 5024 1165
Size: 1149 2710 1648
Fragments arranged by size:
2710 1648 1149
SfcI C'TryA_G
Cuts at: 33 115 688 1383 1805 2111 2550 3235 4228
Size: 82 573 695 422 306 439 685 993
Cuts at: 4228 4419 33
Size: 191 1121
Fragments arranged by size:
1121 993 695 685 573 439 422 306
191 82
SfoI GGC'GCC
Cuts at: 2939 3187 2939
Size: 248 5259
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5507
SmaI CCC'GGG
Cuts at: 2529 2529
Size: 5507
SmlI C'TyrA_G
Cuts at: 169 2010 2340 2662 3577 4069 4331 169
Size: 1841 330 322 915 492 262 1345
Fragments arranged by size:
1841 1345 915 492 330 322 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5507
SphI G_CATG'C
Cuts at: 2195 2560 3060 2195
Size: 365 500 4642
Fragments arranged by size:
4642 500 365
StuI AGG'CCT
Cuts at: 392 661 2410 392
Size: 269 1749 3489
Fragments arranged by size:
3489 1749 269
StyI C'CwwG_G
Cuts at: 107 139 617 1447 2448 2641 2995 107
Size: 32 478 830 1001 193 354 2619
Fragments arranged by size:
2619 1001 830 478 354 193 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 531 1207 2308 2417 3090 3223 3682 3891 531
Size: 676 1101 109 673 133 459 209 2147
Fragments arranged by size:
2147 1101 676 673 459 209 133 109
TatI w'GTAC_w
Cuts at: 1163 1745 2312 5022 1163
Size: 582 567 2710 1648
Fragments arranged by size:
2710 1648 582 567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5024 5056 5024
Size: 32 5475
Tth111I GACn'n_nGTC
Cuts at: 1929 3304 1929
Size: 1375 4132
XbaI T'CTAG_A
Cuts at: 2538 2538
Size: 5507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1779 3002 1779
Size: 1223 4284
XmaI C'CCGG_G
Cuts at: 2527 2527
Size: 5507
XmnI GAAnn'nnTTC
Cuts at: 1410 1583 2272 1410
Size: 173 689 4645
Fragments arranged by size:
4645 689 173
ZraI GAC'GTC
Cuts at: 678 678
Size: 5507
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AleI AlfI AscI BaeI BbvCI BmgBI
Bpu10I BsgI BsiWI BsmBI BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI
NotI NruI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI