Restriction map for
pF1KE2658
(Circular) (Six-base) MAPSORT of: pF1KE2658.seq Check: 5800 from: 1 to: 6509
pF1KE2658 6509 bp
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2875 3275 2875
Size: 400 6109
AatII G_ACGT'C
Cuts at: 3495 3495
Size: 6509
AccI GT'mk_AC
Cuts at: 3547 3547
Size: 6509
Acc65I G'GTAC_C
Cuts at: 2188 3525 2188
Size: 1337 5172
AclI AA'CG_TT
Cuts at: 6349 6349
Size: 6509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 248 880 1633 1676 1940 2144 2762 4334
Size: 150 632 753 43 264 204 618 1572
Cuts at: 4334 4766 5513 5781 98
Size: 432 747 268 826
Fragments arranged by size:
1572 826 753 747 632 618 432 268
264 204 150 43
AflIII A'CryG_T
Cuts at: 3861 4965 5942 3861
Size: 1104 977 4428
Fragments arranged by size:
4428 1104 977
AgeI A'CCGG_T
Cuts at: 4897 4897
Size: 6509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2640 2640
Size: 6509
AlwNI CAG_nnn'CTG
Cuts at: 1029 1207 1793 2391 3814 5381 1029
Size: 178 586 598 1423 1567 2157
Fragments arranged by size:
2157 1567 1423 598 586 178
ApaI G_GGCC'C
Cuts at: 340 1070 1137 340
Size: 730 67 5712
Fragments arranged by size:
5712 730 67
ApaLI G'TGCA_C
Cuts at: 5279 5279
Size: 6509
ApoI r'AATT_y
Cuts at: 722 3513 5862 6118 722
Size: 2791 2349 256 1113
Fragments arranged by size:
2791 2349 1113 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6509
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
AvaI C'yCGr_G
Cuts at: 462 3529 462
Size: 3067 3442
AvrII C'CTAG_G
Cuts at: 705 705
Size: 6509
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2172 2205 2420 2453 2172
Size: 33 215 33 6228
Fragments arranged by size:
6228 215 33 33
BamHI G'GATC_C
Cuts at: 2916 3534 2916
Size: 618 5891
BanI G'GyrC_C
Cuts at: 785 1182 1551 1663 2188 3525 3939 4187 4222
Size: 397 369 112 525 1337 414 248 35
Cuts at: 4222 785
Size: 3072
Fragments arranged by size:
3072 1337 525 414 397 369 248 112
35
BanII G_rGCy'C
Cuts at: 340 410 1070 1137 2398 3336 3384 3523 4553
Size: 70 660 67 1261 938 48 139 1030
Cuts at: 4553 340
Size: 2296
Fragments arranged by size:
2296 1261 1030 938 660 139 70 67
48
BbeI G_GCGC'C
Cuts at: 1555 1667 3943 4191 1555
Size: 112 2276 248 3873
Fragments arranged by size:
3873 2276 248 112
BbsI GAAGACnn'nnnn_
Cuts at: 532 2657 532
Size: 2125 4384
BbvCI CC'TCA_GC
Cuts at: 587 980 587
Size: 393 6116
BciVI GTATCCnnnnn_n'
Cuts at: 1331 2439 4407 5168 6509 1331
Size: 1108 1968 761 1341 1331
Fragments arranged by size:
1968 1341 1331 1108 761
BclI T'GATC_A
Cuts at: 2211 4029 2211
Size: 1818 4691
BglI GCCn_nnn'nGGC
Cuts at: 1929 1929
Size: 6509
BglII A'GATC_T
Cuts at: 1419 4024 6061 1419
Size: 2605 2037 1867
Fragments arranged by size:
2605 2037 1867
BlpI GC'TnA_GC
Cuts at: 404 2278 3621 404
Size: 1874 1343 3292
Fragments arranged by size:
3292 1874 1343
Bme1580I G_kGCm'C
Cuts at: 340 469 629 1070 1137 1934 2147 4134 4227
Size: 129 160 441 67 797 213 1987 93
Cuts at: 4227 5283 340
Size: 1056 1566
Fragments arranged by size:
1987 1566 1056 797 441 213 160 129
93 67
BmrI ACTGGGnnnn_n'
Cuts at: 829 1271 2003 2401 2597 2668 2990 3721 3802
Size: 442 732 398 196 71 322 731 81
Cuts at: 3802 4137 6281 829
Size: 335 2144 1057
Fragments arranged by size:
2144 1057 732 731 442 398 335 322
196 81 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2745 3174 4876 2745
Size: 429 1702 4378
Fragments arranged by size:
4378 1702 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 557 898 2382 2444 3299 3362 260
Size: 297 341 1484 62 855 63 3407
Fragments arranged by size:
3407 1484 855 341 297 63 62
Bpu10I CC'TnA_GC
Cuts at: 587 980 1339 587
Size: 393 359 5757
Fragments arranged by size:
5757 393 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1569 2105 2574 3685 4564 5056 5354 505
Size: 1064 536 469 1111 879 492 298 1660
Fragments arranged by size:
1660 1111 1064 879 536 492 469 298
BsaI GGTCTCn'nnnn_
Cuts at: 1025 1158 1339 6213 1025
Size: 133 181 4874 1321
Fragments arranged by size:
4874 1321 181 133
BsaAI yAC'GTr
Cuts at: 3862 3862
Size: 6509
BsaBI GATnn'nnATC
Cuts at: 6 1904 2216 2921 4023 6060 6
Size: 1898 312 705 1102 2037 455
Fragments arranged by size:
2037 1898 1102 705 455 312
BsaHI Gr'CG_yC
Cuts at: 1146 1552 1664 3492 3940 4188 4890 6386 1146
Size: 406 112 1828 448 248 702 1496 1269
Fragments arranged by size:
1828 1496 1269 702 448 406 248 112
BsaWI w'CCGG_w
Cuts at: 141 932 3698 4219 4897 5171 5318 5729 141
Size: 791 2766 521 678 274 147 411 921
Fragments arranged by size:
2766 921 791 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 665 695 2465 2495 2970 3000 665
Size: 30 1770 30 475 30 4174
Fragments arranged by size:
4174 1770 475 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 895 997 1321 1480 1549 1869 2030 2341 2721
Size: 102 324 159 69 320 161 311 380
Cuts at: 2721 2774 895
Size: 53 4630
Fragments arranged by size:
4630 380 324 320 311 161 159 102
69 53
BseYI C'CCAG_C
Cuts at: 307 355 1499 1657 1786 1793 1918 2236 3292
Size: 48 1144 158 129 7 125 318 1056
Cuts at: 3292 3934 4692 5269 5765 307
Size: 642 758 577 496 1051
Fragments arranged by size:
1144 1056 1051 758 642 577 496 318
158 129 125 48 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 479 922 170
Size: 309 443 5757
Fragments arranged by size:
5757 443 309
BsiEI CG_ry'CG
Cuts at: 104 4051 4097 5305 104
Size: 3947 46 1208 1308
Fragments arranged by size:
3947 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 917 2005 3336 3384 3523 4301 4491 5283 917
Size: 1088 1331 48 139 778 190 792 2143
Fragments arranged by size:
2143 1331 1088 792 778 190 139 48
BsmI GAATG_Cn'
Cuts at: 3054 3054
Size: 6509
Bsp1286I G_dGCh'C
Cuts at: 340 410 469 629 917 1070 1137 1934 2005
Size: 70 59 160 288 153 67 797 71
Cuts at: 2005 2147 2398 3336 3384 3523 4134 4227 4301
Size: 142 251 938 48 139 611 93 74
Cuts at: 4301 4491 4553 5283 340
Size: 190 62 730 1566
Fragments arranged by size:
1566 938 797 730 611 288 251 190
160 153 142 139 93 74 71 70
67 62 59 48
BspEI T'CCGG_A
Cuts at: 5729 5729
Size: 6509
BspHI T'CATG_A
Cuts at: 5685 5685
Size: 6509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1808 2875 3275 3559 3773 4075 4456 1808
Size: 1067 400 284 214 302 381 3861
Fragments arranged by size:
3861 1067 400 381 302 284 214
BsrBI CCG'CTC
Cuts at: 50 716 4801 4855 6339 6504 50
Size: 666 4085 54 1484 165 55
Fragments arranged by size:
4085 1484 666 165 55 54
BsrDI GCAATG_nn'
Cuts at: 130 2632 4421 130
Size: 2502 1789 2218
Fragments arranged by size:
2502 2218 1789
BsrFI r'CCGG_y
Cuts at: 1142 4507 4688 4897 1142
Size: 3365 181 209 2754
Fragments arranged by size:
3365 2754 209 181
BsrGI T'GTAC_A
Cuts at: 1100 2316 1100
Size: 1216 5293
BssSI C'ACGA_G
Cuts at: 4780 5138 4780
Size: 358 6151
BstAPI GCAn_nnn'nTGC
Cuts at: 3886 3886
Size: 6509
BstBI TT'CG_AA
Cuts at: 4870 4870
Size: 6509
BstEII G'GTnAC_C
Cuts at: 709 709
Size: 6509
BstXI CCAn_nnnn'nTGG
Cuts at: 2273 3016 2273
Size: 743 5766
BstYI r'GATC_y
Cuts at: 1 1193 1326 1419 1446 2916 3534 4001 4024
Size: 1192 133 93 27 1470 618 467 23
Cuts at: 4024 4359 4605 5617 5703 5715 6061 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1470 1192 1012 618 467 449 346 335
246 133 93 86 27 23 12
Bsu36I CC'TnA_GG
Cuts at: 1178 3234 1178
Size: 2056 4453
BtgI C'CryG_G
Cuts at: 107 2195 107
Size: 2088 4421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4459 4640 4803 4459
Size: 181 163 6165
Fragments arranged by size:
6165 181 163
BtsI GCAGTG_nn'
Cuts at: 1932 2147 2289 2463 3752 1932
Size: 215 142 174 1289 4689
Fragments arranged by size:
4689 1289 215 174 142
DraI TTT'AAA
Cuts at: 3508 3508
Size: 6509
DrdI GACnn_nn'nnGTC
Cuts at: 3466 4215 5073 3466
Size: 749 858 4902
Fragments arranged by size:
4902 858 749
EaeI y'GGCC_r
Cuts at: 643 683 1256 1902 4094 4268 4659 4686 6031
Size: 40 573 646 2192 174 391 27 1345
Cuts at: 6031 643
Size: 1121
Fragments arranged by size:
2192 1345 1121 646 573 391 174 40
27
EagI C'GGCC_G
Cuts at: 4094 4094
Size: 6509
EarI CTCTTCn'nnn_
Cuts at: 1526 2163 3137 4532 4742 1526
Size: 637 974 1395 210 3293
Fragments arranged by size:
3293 1395 974 637 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 572 4717 5027 5173 6319 572
Size: 4145 310 146 1146 762
Fragments arranged by size:
4145 1146 762 310 146
EcoICRI GAG'CTC
Cuts at: 3334 3382 3521 3334
Size: 48 139 6322
Fragments arranged by size:
6322 139 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 248 260 557 880 898 1633 1676 1940
Size: 150 12 297 323 18 735 43 264
Cuts at: 1940 2144 2382 2444 2762 3299 3362 4334 4766
Size: 204 238 62 318 537 63 972 432
Cuts at: 4766 5513 5781 98
Size: 747 268 826
Fragments arranged by size:
972 826 747 735 537 432 323 318
297 268 264 238 204 150 63 62
43 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 704 880 1678 2045 704
Size: 176 798 367 5168
Fragments arranged by size:
5168 798 367 176
EcoO109I rG'GnC_Cy
Cuts at: 336 337 490 568 676 1026 1066 1067 1134
Size: 1 153 78 108 350 40 1 67
Cuts at: 1134 1797 1837 2419 2527 3012 3289 3393 3648
Size: 663 40 582 108 485 277 104 255
Cuts at: 3648 336
Size: 3197
Fragments arranged by size:
3197 663 582 485 350 277 255 153
108 108 104 78 67 40 40 1
1
EcoRI G'AATT_C
Cuts at: 3513 3513
Size: 6509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2695 4528 2695
Size: 1833 4676
FspI TGC'GCA
Cuts at: 4290 4290
Size: 6509
HaeII r_GCGC'y
Cuts at: 1555 1574 1667 1919 3943 4191 5213 6192 1555
Size: 19 93 252 2024 248 1022 979 1872
Fragments arranged by size:
2024 1872 1022 979 252 248 93 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 1411 1443 2196 2228 2716 2748 4351
Size: 32 1270 32 753 32 488 32 1603
Cuts at: 4351 4381 4383 4413 109
Size: 30 2 30 2205
Fragments arranged by size:
2205 1603 1270 753 488 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1224 2811 3548 1224
Size: 1587 737 4185
Fragments arranged by size:
4185 1587 737
HindIII A'AGCT_T
Cuts at: 638 638
Size: 6509
KasI G'GCGC_C
Cuts at: 1551 1663 3939 4187 1551
Size: 112 2276 248 3873
Fragments arranged by size:
3873 2276 248 112
KpnI G_GTAC'C
Cuts at: 2192 3529 2192
Size: 1337 5172
MluI A'CGCG_T
Cuts at: 5942 5942
Size: 6509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1559 2771 2868 3185 5180 5364 1559
Size: 1212 97 317 1995 184 2704
Fragments arranged by size:
2704 1995 1212 317 184 97
MscI TGG'CCA
Cuts at: 645 685 1258 1904 4270 645
Size: 40 573 646 2366 2884
Fragments arranged by size:
2884 2366 646 573 40
MslI CAynn'nnrTG
Cuts at: 1763 1898 2603 2688 2888 4498 4625 1763
Size: 135 705 85 200 1610 127 3647
Fragments arranged by size:
3647 1610 705 200 135 127 85
MspA1I CmG'CkG
Cuts at: 403 1008 1503 1793 2236 2491 3620 3934 4294
Size: 605 495 290 443 255 1129 314 360
Cuts at: 4294 5307 5552 403
Size: 1013 245 1360
Fragments arranged by size:
1360 1129 1013 605 495 443 360 314
290 255 245
NaeI GCC'GGC
Cuts at: 1144 4690 1144
Size: 3546 2963
NarI GG'CG_CC
Cuts at: 1552 1664 3940 4188 1552
Size: 112 2276 248 3873
Fragments arranged by size:
3873 2276 248 112
NcoI C'CATG_G
Cuts at: 107 2195 107
Size: 2088 4421
NgoMIV G'CCGG_C
Cuts at: 1142 4688 1142
Size: 3546 2963
NspI r_CATG'y
Cuts at: 2693 3562 4062 4969 2693
Size: 869 500 907 4233
Fragments arranged by size:
4233 907 869 500
PasI CC'CwG_GG
Cuts at: 233 2147 2415 3398 233
Size: 1914 268 983 3344
Fragments arranged by size:
3344 1914 983 268
PciI A'CATG_T
Cuts at: 4965 4965
Size: 6509
PflMI CCAn_nnn'nTGG
Cuts at: 1793 2391 1793
Size: 598 5911
PfoI T'CCnGG_A
Cuts at: 1833 2421 1833
Size: 588 5921
PmeI GTTT'AAAC
Cuts at: 3508 3508
Size: 6509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5648 5680 5648
Size: 32 6477
PpuMI rG'GwC_Cy
Cuts at: 490 568 676 1797 1837 2419 2527 3393 490
Size: 78 108 1121 40 582 108 866 3606
Fragments arranged by size:
3606 1121 866 582 108 108 78 40
PshAI GACnn'nnGTC
Cuts at: 1586 1586
Size: 6509
PsiI TTA'TAA
Cuts at: 1876 1876
Size: 6509
PspOMI G'GGCC_C
Cuts at: 336 1066 1133 336
Size: 730 67 5712
Fragments arranged by size:
5712 730 67
PstI C_TGCA'G
Cuts at: 1805 3556 4241 1805
Size: 1751 685 4073
Fragments arranged by size:
4073 1751 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6509
PvuII CAG'CTG
Cuts at: 403 1008 1503 1793 2236 2491 3934 4294 403
Size: 605 495 290 443 255 1443 360 2618
Fragments arranged by size:
2618 1443 605 495 443 360 290 255
RsrII CG'GwC_CG
Cuts at: 4704 4704
Size: 6509
SacI G_AGCT'C
Cuts at: 3336 3384 3523 3336
Size: 48 139 6322
Fragments arranged by size:
6322 139 48
SalI G'TCGA_C
Cuts at: 3546 3546
Size: 6509
SanDI GG'GwC_CC
Cuts at: 1837 3393 1837
Size: 1556 4953
SapI GCTCTTCn'nnn_
Cuts at: 2163 4532 4742 2163
Size: 2369 210 3930
Fragments arranged by size:
3930 2369 210
SbfI CC_TGCA'GG
Cuts at: 3556 3556
Size: 6509
ScaI AGT'ACT
Cuts at: 6026 6026
Size: 6509
SexAI A'CCwGG_T
Cuts at: 1880 1880
Size: 6509
SfcI C'TryA_G
Cuts at: 33 1801 2297 3552 4237 5230 5421 33
Size: 1768 496 1255 685 993 191 1121
Fragments arranged by size:
1768 1255 1121 993 685 496 191
SfoI GGC'GCC
Cuts at: 1553 1665 3941 4189 1553
Size: 112 2276 248 3873
Fragments arranged by size:
3873 2276 248 112
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
SgrAI Cr'CCGG_yG
Cuts at: 1142 1142
Size: 6509
SmaI CCC'GGG
Cuts at: 464 3531 464
Size: 3067 3442
SmlI C'TyrA_G
Cuts at: 484 1584 2120 2553 3664 4579 5071 5333 484
Size: 1100 536 433 1111 915 492 262 1660
Fragments arranged by size:
1660 1111 1100 915 536 492 433 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6509
SphI G_CATG'C
Cuts at: 3562 4062 3562
Size: 500 6009
StuI AGG'CCT
Cuts at: 482 578 482
Size: 96 6413
StyI C'CwwG_G
Cuts at: 107 705 728 773 1187 1382 1451 1526 1674
Size: 598 23 45 414 195 69 75 148
Cuts at: 1674 1841 2195 2694 2898 3015 3202 3643 3997
Size: 167 354 499 204 117 187 441 354
Cuts at: 3997 107
Size: 2619
Fragments arranged by size:
2619 598 499 441 414 354 354 204
195 187 167 148 117 75 69 45
23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 242 246 1200 1834 2700 4092 4225 4684 4893
Size: 4 954 634 866 1392 133 459 209
Cuts at: 4893 242
Size: 1858
Fragments arranged by size:
1858 1392 954 866 634 459 209 133
4
TatI w'GTAC_w
Cuts at: 1100 2316 6024 1100
Size: 1216 3708 1585
Fragments arranged by size:
3708 1585 1216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 665 697 6026 6058 665
Size: 32 5329 32 1116
Fragments arranged by size:
5329 1116 32 32
Tth111I GACn'n_nGTC
Cuts at: 2251 4306 2251
Size: 2055 4454
XbaI T'CTAG_A
Cuts at: 3540 3540
Size: 6509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 315 627 873 4004 315
Size: 312 246 3131 2820
Fragments arranged by size:
3131 2820 312 246
XmaI C'CCGG_G
Cuts at: 462 3529 462
Size: 3067 3442
XmnI GAAnn'nnTTC
Cuts at: 1683 2678 1683
Size: 995 5514
ZraI GAC'GTC
Cuts at: 3493 3493
Size: 6509
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI BcgI BfrBI
BmgBI BmtI BsiWI BsmBI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PspXI PsrI SacII
SfiI SnaBI SrfI SspI SwaI XhoI