Restriction map for
pF1KE2657
(Circular) (Six-base) MAPSORT of: pF1KE2657.seq Check: 8681 from: 1 to: 3704
pF1KE2657 3704 bp
With 182 enzymes: SgfI *
December 21, 2016 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 472 472
Size: 3704
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1529 1961 2708 2976 1529
Size: 432 747 268 2257
Fragments arranged by size:
2257 747 432 268
AflIII A'CryG_T
Cuts at: 1056 2160 3137 1056
Size: 1104 977 1623
Fragments arranged by size:
1623 1104 977
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AlwNI CAG_nnn'CTG
Cuts at: 432 435 457 1009 2576 432
Size: 3 22 552 1567 1560
Fragments arranged by size:
1567 1560 552 22 3
ApaLI G'TGCA_C
Cuts at: 199 2474 199
Size: 2275 1429
ApoI r'AATT_y
Cuts at: 708 3057 3313 708
Size: 2349 256 1099
Fragments arranged by size:
2349 1099 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 167 724 167
Size: 557 3147
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 330 460 681 720 1134 1382 1417 330
Size: 130 221 39 414 248 35 2617
Fragments arranged by size:
2617 414 248 221 130 39 35
BanII G_rGCy'C
Cuts at: 145 267 718 1748 145
Size: 122 451 1030 2101
Fragments arranged by size:
2101 1030 451 122
BbeI G_GCGC'C
Cuts at: 334 1138 1386 334
Size: 804 248 2652
Fragments arranged by size:
2652 804 248
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglI GCCn_nnn'nGGC
Cuts at: 179 179
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 618 816 618
Size: 198 3506
Bme1580I G_kGCm'C
Cuts at: 203 1329 1422 2478 203
Size: 1126 93 1056 1429
Fragments arranged by size:
1429 1126 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 190 497 916 997 1332 3476 190
Size: 307 419 81 335 2144 418
Fragments arranged by size:
2144 419 418 335 307 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 286 2071 286
Size: 1785 1919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 473 334
Size: 139 3565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 880 1759 2251 2549 529
Size: 351 879 492 298 1684
Fragments arranged by size:
1684 879 492 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 3408 283
Size: 3125 579
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 331 1135 1383 2085 3581 331
Size: 804 248 702 1496 454
Fragments arranged by size:
1496 804 702 454 248
BsaWI w'CCGG_w
Cuts at: 628 893 1414 2092 2366 2513 2924 628
Size: 265 521 678 274 147 411 1408
Fragments arranged by size:
1408 678 521 411 274 265 147
BseYI C'CCAG_C
Cuts at: 341 588 1129 1887 2464 2960 341
Size: 247 541 758 577 496 1085
Fragments arranged by size:
1085 758 577 541 496 247
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2500 104
Size: 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 46
BsiHKAI G_wGCw'C
Cuts at: 203 368 718 1496 1686 2478 203
Size: 165 350 778 190 792 1429
Fragments arranged by size:
1429 792 778 350 190 165
BsmI GAATG_Cn'
Cuts at: 182 553 182
Size: 371 3333
Bsp1286I G_dGCh'C
Cuts at: 145 203 267 368 718 1329 1422 1496 1686
Size: 58 64 101 350 611 93 74 190
Cuts at: 1686 1748 2478 145
Size: 62 730 1371
Fragments arranged by size:
1371 730 611 350 190 101 93 74
64 62 58
BspEI T'CCGG_A
Cuts at: 628 2924 628
Size: 2296 1408
BspHI T'CATG_A
Cuts at: 476 2880 476
Size: 2404 1300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 472 754 968 1270 1651 472
Size: 282 214 302 381 2525
Fragments arranged by size:
2525 381 302 282 214
BsrBI CCG'CTC
Cuts at: 50 687 1996 2050 3534 3699 50
Size: 637 1309 54 1484 165 55
Fragments arranged by size:
1484 1309 637 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 1616
Size: 3704
BsrFI r'CCGG_y
Cuts at: 1702 1883 2092 1702
Size: 181 209 3314
Fragments arranged by size:
3314 209 181
BssSI C'ACGA_G
Cuts at: 409 1975 2333 409
Size: 1566 358 1780
Fragments arranged by size:
1780 1566 358
BstAPI GCAn_nnn'nTGC
Cuts at: 188 467 1081 188
Size: 279 614 2811
Fragments arranged by size:
2811 614 279
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstEII G'GTnAC_C
Cuts at: 372 372
Size: 3704
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3704
BstYI r'GATC_y
Cuts at: 1 729 1196 1219 1554 1800 2812 2898 2910
Size: 728 467 23 335 246 1012 86 12
Cuts at: 2910 3256 1
Size: 346 449
Fragments arranged by size:
1012 728 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 377 578 947 377
Size: 201 369 3134
Fragments arranged by size:
3134 369 201
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DraIII CAC_nnn'GTG
Cuts at: 671 671
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 656 1289 1463 1854 1881 3226 656
Size: 633 174 391 27 1345 1134
Fragments arranged by size:
1345 1134 633 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1912 2222 2368 3514 1912
Size: 310 146 1146 2102
Fragments arranged by size:
2102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 716
Size: 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 473 1529 1961 2708 2976 334
Size: 139 1056 432 747 268 1062
Fragments arranged by size:
1062 1056 747 432 268 139
EcoO109I rG'GnC_Cy
Cuts at: 129 636 843 129
Size: 507 207 2990
Fragments arranged by size:
2990 507 207
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1723 1723
Size: 3704
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 334 1138 1386 2408 3387 334
Size: 804 248 1022 979 651
Fragments arranged by size:
1022 979 804 651 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 1546 1576 1578 1608 601
Size: 32 913 30 2 30 2697
Fragments arranged by size:
2697 913 32 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
KasI G'GCGC_C
Cuts at: 330 1134 1382 330
Size: 804 248 2652
Fragments arranged by size:
2652 804 248
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2559 2375
Size: 184 3520
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 112 247 304 658 1693 1820 112
Size: 135 57 354 1035 127 1996
Fragments arranged by size:
1996 1035 354 135 127 57
MspA1I CmG'CkG
Cuts at: 432 815 1129 1489 2502 2747 432
Size: 383 314 360 1013 245 1389
Fragments arranged by size:
1389 1013 383 360 314 245
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 331 1135 1383 331
Size: 804 248 2652
Fragments arranged by size:
2652 804 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3704
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 757 1257 2164 757
Size: 500 907 2297
Fragments arranged by size:
2297 907 500
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PflMI CCAn_nnn'nTGG
Cuts at: 457 457
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PpuMI rG'GwC_Cy
Cuts at: 129 636 129
Size: 507 3197
PstI C_TGCA'G
Cuts at: 560 751 1436 560
Size: 191 685 2828
Fragments arranged by size:
2828 685 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 432 1129 1489 432
Size: 697 360 2647
Fragments arranged by size:
2647 697 360
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 718 718
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SanDI GG'GwC_CC
Cuts at: 636 636
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SexAI A'CCwGG_T
Cuts at: 368 368
Size: 3704
SfcI C'TryA_G
Cuts at: 33 556 747 1432 2425 2616 33
Size: 523 191 685 993 191 1121
Fragments arranged by size:
1121 993 685 523 191 191
SfoI GGC'GCC
Cuts at: 332 1136 1384 332
Size: 804 248 2652
Fragments arranged by size:
2652 804 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 169 726 169
Size: 557 3147
SmlI C'TyrA_G
Cuts at: 508 859 1774 2266 2528 508
Size: 351 915 492 262 1684
Fragments arranged by size:
1684 915 492 351 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
StyI C'CwwG_G
Cuts at: 107 838 1192 107
Size: 731 354 2619
Fragments arranged by size:
2619 731 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1287 1420 1879 2088 1287
Size: 133 459 209 2903
Fragments arranged by size:
2903 459 209 133
TatI w'GTAC_w
Cuts at: 3219 3219
Size: 3704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 408 1199 408
Size: 791 2913
XmaI C'CCGG_G
Cuts at: 167 724 167
Size: 557 3147
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI