Restriction map for
pF1KE2656
(Circular) (Six-base) MAPSORT of: pF1KE2656.seq Check: 5310 from: 1 to: 3905
pF1KE2656 3905 bp
With 182 enzymes: SgfI *
December 1, 2016 14:58 ..
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 527 921 527
Size: 394 3511
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 775 1730 2162 2909 3177 353
Size: 422 955 432 747 268 1081
Fragments arranged by size:
1081 955 747 432 422 268
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 175 449 829 1210 2777 175
Size: 274 380 381 1567 1303
Fragments arranged by size:
1567 1303 381 380 274
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 306 724 909 3258 3514 306
Size: 418 185 2349 256 697
Fragments arranged by size:
2349 697 418 256 185
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 925 925
Size: 3905
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 527 921 1335 1583 1618 527
Size: 394 414 248 35 2814
Fragments arranged by size:
2814 414 394 248 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BbsI GAAGACnn'nnnn_
Cuts at: 874 874
Size: 3905
BciVI GTATCCnnnnn_n'
Cuts at: 652 1803 2564 3905 652
Size: 1151 761 1341 652
Fragments arranged by size:
1341 1151 761 652
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BfrBI ATG'CAT
Cuts at: 757 757
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 1530 1623 2679 1530
Size: 93 1056 2756
Fragments arranged by size:
2756 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 594 1117 1198 1533 3677 594
Size: 523 81 335 2144 822
Fragments arranged by size:
2144 822 523 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1960 2452 2750 1081
Size: 879 492 298 2236
Fragments arranged by size:
2236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 1336 1584 2286 3782 1336
Size: 248 702 1496 1459
Fragments arranged by size:
1496 1459 702 248
BsaWI w'CCGG_w
Cuts at: 524 1094 1615 2293 2567 2714 3125 524
Size: 570 521 678 274 147 411 1304
Fragments arranged by size:
1304 678 570 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 671 671
Size: 3905
BseYI C'CCAG_C
Cuts at: 1330 2088 2665 3161 1330
Size: 758 577 496 2074
Fragments arranged by size:
2074 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 875
Size: 3905
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 919 1697 1887 2679 919
Size: 778 190 792 2145
Fragments arranged by size:
2145 792 778 190
BsmI GAATG_Cn'
Cuts at: 611 647 611
Size: 36 3869
Bsp1286I G_dGCh'C
Cuts at: 919 1530 1623 1697 1887 1949 2679 919
Size: 611 93 74 190 62 730 2145
Fragments arranged by size:
2145 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1169 1471 1852 955
Size: 214 302 381 3008
Fragments arranged by size:
3008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 206 343 1817 206
Size: 137 1474 2294
Fragments arranged by size:
2294 1474 137
BsrFI r'CCGG_y
Cuts at: 145 605 1903 2084 2293 145
Size: 460 1298 181 209 1757
Fragments arranged by size:
1757 1298 460 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstXI CCAn_nnnn'nTGG
Cuts at: 857 857
Size: 3905
BstYI r'GATC_y
Cuts at: 1 204 379 930 1397 1420 1755 2001 3013
Size: 203 175 551 467 23 335 246 1012
Cuts at: 3013 3099 3111 3457 1
Size: 86 12 346 449
Fragments arranged by size:
1012 551 467 449 346 335 246 203
175 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 827 1855 2036 2199 827
Size: 1028 181 163 2533
Fragments arranged by size:
2533 1028 181 163
BtsI GCAGTG_nn'
Cuts at: 450 882 1148 450
Size: 432 266 3207
Fragments arranged by size:
3207 432 266
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 189 1490 1664 2055 2082 3427 189
Size: 1301 174 391 27 1345 667
Fragments arranged by size:
1345 1301 667 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 775 1730 2162 2909 3177 353
Size: 422 955 432 747 268 1081
Fragments arranged by size:
1081 955 747 432 422 268
EcoO109I rG'GnC_Cy
Cuts at: 1044 1044
Size: 3905
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 1339 1587 2609 3588 1339
Size: 248 1022 979 1656
Fragments arranged by size:
1656 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 647 679 1747 1777 1779 1809 647
Size: 32 1068 30 2 30 2743
Fragments arranged by size:
2743 1068 32 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 531 925 531
Size: 394 3511
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 159 255 2576 2760 159
Size: 96 2321 184 1304
Fragments arranged by size:
2321 1304 184 96
MscI TGG'CCA
Cuts at: 191 1666 191
Size: 1475 2430
MslI CAynn'nnrTG
Cuts at: 1894 2021 1894
Size: 127 3778
MspA1I CmG'CkG
Cuts at: 1016 1330 1690 2703 2948 1016
Size: 314 360 1013 245 1973
Fragments arranged by size:
1973 1013 360 314 245
NaeI GCC'GGC
Cuts at: 147 2086 147
Size: 1939 1966
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NdeI CA'TA_TG
Cuts at: 288 288
Size: 3905
NgoMIV G'CCGG_C
Cuts at: 145 2084 145
Size: 1939 1966
NsiI A_TGCA'T
Cuts at: 759 759
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PflMI CCAn_nnn'nTGG
Cuts at: 274 274
Size: 3905
PfoI T'CCnGG_A
Cuts at: 651 651
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 449 481 3044 3076 449
Size: 32 2563 32 1278
Fragments arranged by size:
2563 1278 32 32
PstI C_TGCA'G
Cuts at: 506 952 1637 506
Size: 446 685 2774
Fragments arranged by size:
2774 685 446
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 1330 1690 1330
Size: 360 3545
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 516 3422 516
Size: 2906 999
SfcI C'TryA_G
Cuts at: 33 370 502 948 1633 2626 2817 33
Size: 337 132 446 685 993 191 1121
Fragments arranged by size:
1121 993 685 446 337 191 132
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 1060 1975 2467 2729 1060
Size: 915 492 262 2236
Fragments arranged by size:
2236 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
StyI C'CwwG_G
Cuts at: 1039 1393 1039
Size: 354 3551
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 1488 1621 2080 2289 423
Size: 1065 133 459 209 2039
Fragments arranged by size:
2039 1065 459 209 133
TatI w'GTAC_w
Cuts at: 514 3420 514
Size: 2906 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1400 1400
Size: 3905
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI