Restriction map for
pF1KE2655
(Circular) (Six-base) MAPSORT of: pF1KE2655.seq Check: 6436 from: 1 to: 4436
pF1KE2655 4436 bp
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 1034 1367 1474 1034
Size: 333 107 3996
Fragments arranged by size:
3996 333 107
Acc65I G'GTAC_C
Cuts at: 1452 1452
Size: 4436
AclI AA'CG_TT
Cuts at: 4276 4276
Size: 4436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 886 925 2261 2693 3440 3708 437
Size: 449 39 1336 432 747 268 1165
Fragments arranged by size:
1336 1165 747 449 432 268 39
AflIII A'CryG_T
Cuts at: 1002 1313 1788 2892 3869 1002
Size: 311 475 1104 977 1569
Fragments arranged by size:
1569 1104 977 475 311
AgeI A'CCGG_T
Cuts at: 2824 2824
Size: 4436
AhdI GACnn_n'nnGTC
Cuts at: 1401 1401
Size: 4436
AlwNI CAG_nnn'CTG
Cuts at: 1741 3308 1741
Size: 1567 2869
ApaLI G'TGCA_C
Cuts at: 3206 3206
Size: 4436
ApoI r'AATT_y
Cuts at: 1440 3789 4045 1440
Size: 2349 256 1831
Fragments arranged by size:
2349 1831 256
AseI AT'TA_AT
Cuts at: 20 941 20
Size: 921 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 767 1008 1456 767
Size: 241 448 3747
Fragments arranged by size:
3747 448 241
BamHI G'GATC_C
Cuts at: 1461 1461
Size: 4436
BanI G'GyrC_C
Cuts at: 1452 1866 2114 2149 1452
Size: 414 248 35 3739
Fragments arranged by size:
3739 414 248 35
BanII G_rGCy'C
Cuts at: 119 289 360 776 1450 2480 119
Size: 170 71 416 674 1030 2075
Fragments arranged by size:
2075 1030 674 416 170 71
BbeI G_GCGC'C
Cuts at: 1870 2118 1870
Size: 248 4188
BbsI GAAGACnn'nnnn_
Cuts at: 458 809 458
Size: 351 4085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1387 1421 1387
Size: 34 4402
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3095 4436 2334
Size: 761 1341 2334
Fragments arranged by size:
2334 1341 761
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BglI GCCn_nnn'nGGC
Cuts at: 538 596 1414 538
Size: 58 818 3560
Fragments arranged by size:
3560 818 58
BglII A'GATC_T
Cuts at: 1951 3988 1951
Size: 2037 2399
BlpI GC'TnA_GC
Cuts at: 113 1548 113
Size: 1435 3001
Bme1580I G_kGCm'C
Cuts at: 854 2061 2154 3210 854
Size: 1207 93 1056 2080
Fragments arranged by size:
2080 1207 1056 93
BmgBI CAC'GTC
Cuts at: 1005 1194 1316 1005
Size: 189 122 4125
Fragments arranged by size:
4125 189 122
BmrI ACTGGGnnnn_n'
Cuts at: 1648 1729 2064 4208 1648
Size: 81 335 2144 1876
Fragments arranged by size:
2144 1876 335 81
BmtI G_CTAG'C
Cuts at: 1393 1393
Size: 4436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 569
Size: 4436
Bpu10I CC'TnA_GC
Cuts at: 1051 1051
Size: 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1612 2491 2983 3281 1612
Size: 879 492 298 2767
Fragments arranged by size:
2767 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4140 4140
Size: 4436
BsaAI yAC'GTr
Cuts at: 471 1789 471
Size: 1318 3118
BsaBI GATnn'nnATC
Cuts at: 6 1950 3987 6
Size: 1944 2037 455
Fragments arranged by size:
2037 1944 455
BsaHI Gr'CG_yC
Cuts at: 1867 2115 2817 4313 1867
Size: 248 702 1496 1990
Fragments arranged by size:
1990 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1625 2146 2824 3098 3245 3656 1625
Size: 521 678 274 147 411 2405
Fragments arranged by size:
2405 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1270 1300 1270
Size: 30 4406
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1332 1332
Size: 4436
BseYI C'CCAG_C
Cuts at: 151 442 655 1376 1861 2619 3196 3692 151
Size: 291 213 721 485 758 577 496 895
Fragments arranged by size:
895 758 721 577 496 485 291 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 952
Size: 4436
BsiEI CG_ry'CG
Cuts at: 104 1978 2024 3232 104
Size: 1874 46 1208 1308
Fragments arranged by size:
1874 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 313 360 1450 2228 2418 3210 313
Size: 47 1090 778 190 792 1539
Fragments arranged by size:
1539 1090 792 778 190 47
BsiWI C'GTAC_G
Cuts at: 158 158
Size: 4436
BsmBI CGTCTCn'nnnn_
Cuts at: 1322 1322
Size: 4436
Bsp1286I G_dGCh'C
Cuts at: 119 289 313 360 776 854 1450 2061 2154
Size: 170 24 47 416 78 596 611 93
Cuts at: 2154 2228 2418 2480 3210 119
Size: 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 596 416 190 170 93
78 74 62 47 24
BspEI T'CCGG_A
Cuts at: 3656 3656
Size: 4436
BspHI T'CATG_A
Cuts at: 3612 3612
Size: 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1486 1700 2002 2383 1486
Size: 214 302 381 3539
Fragments arranged by size:
3539 381 302 214
BsrBI CCG'CTC
Cuts at: 50 640 734 2728 2782 4266 4431 50
Size: 590 94 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 590 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 1341 2348 1341
Size: 1007 3429
BsrFI r'CCGG_y
Cuts at: 1348 2434 2615 2824 1348
Size: 1086 181 209 2960
Fragments arranged by size:
2960 1086 209 181
BsrGI T'GTAC_A
Cuts at: 1286 1286
Size: 4436
BssSI C'ACGA_G
Cuts at: 2707 3065 2707
Size: 358 4078
BstAPI GCAn_nnn'nTGC
Cuts at: 1813 1813
Size: 4436
BstBI TT'CG_AA
Cuts at: 131 2797 131
Size: 2666 1770
BstEII G'GTnAC_C
Cuts at: 1059 1059
Size: 4436
BstXI CCAn_nnnn'nTGG
Cuts at: 783 1198 783
Size: 415 4021
BstYI r'GATC_y
Cuts at: 1 271 395 954 1461 1928 1951 2286 2532
Size: 270 124 559 507 467 23 335 246
Cuts at: 2532 3544 3630 3642 3988 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 559 507 467 449 346 335 270
246 124 86 23 12
BtgI C'CryG_G
Cuts at: 107 568 1269 1321 107
Size: 461 701 52 3222
Fragments arranged by size:
3222 701 461 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 533 2386 2567 2730 533
Size: 1853 181 163 2239
Fragments arranged by size:
2239 1853 181 163
BtsI GCAGTG_nn'
Cuts at: 794 1026 1679 794
Size: 232 653 3551
Fragments arranged by size:
3551 653 232
DraI TTT'AAA
Cuts at: 1435 1435
Size: 4436
DrdI GACnn_nn'nnGTC
Cuts at: 2142 3000 2142
Size: 858 3578
EaeI y'GGCC_r
Cuts at: 1110 1415 2021 2195 2586 2613 3958 1110
Size: 305 606 174 391 27 1345 1588
Fragments arranged by size:
1588 1345 606 391 305 174 27
EagI C'GGCC_G
Cuts at: 2021 2021
Size: 4436
EarI CTCTTCn'nnn_
Cuts at: 2459 2669 2459
Size: 210 4226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 810 1217 1291 2644 2954 3100 4246 810
Size: 407 74 1353 310 146 1146 1000
Fragments arranged by size:
1353 1146 1000 407 310 146 74
EcoICRI GAG'CTC
Cuts at: 358 1448 358
Size: 1090 3346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 569 886 925 2261 2693 3440 3708 437
Size: 132 317 39 1336 432 747 268 1165
Fragments arranged by size:
1336 1165 747 432 317 268 132 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1054 1202 1054
Size: 148 4288
EcoO109I rG'GnC_Cy
Cuts at: 1172 1575 1172
Size: 403 4033
EcoRI G'AATT_C
Cuts at: 1440 1440
Size: 4436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1158 1254 2455 1158
Size: 96 1201 3139
Fragments arranged by size:
3139 1201 96
FspI TGC'GCA
Cuts at: 2217 2217
Size: 4436
HaeII r_GCGC'y
Cuts at: 1870 2118 3140 4119 1870
Size: 248 1022 979 2187
Fragments arranged by size:
2187 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 129 161 256 288 465 497 1387 1419 2278
Size: 32 95 32 177 32 890 32 859
Cuts at: 2278 2308 2310 2340 129
Size: 30 2 30 2225
Fragments arranged by size:
2225 890 859 177 95 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1047 1475 1047
Size: 428 4008
HindIII A'AGCT_T
Cuts at: 1135 1135
Size: 4436
KasI G'GCGC_C
Cuts at: 1866 2114 1866
Size: 248 4188
KpnI G_GTAC'C
Cuts at: 1456 1456
Size: 4436
MluI A'CGCG_T
Cuts at: 3869 3869
Size: 4436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 340 778 3107 3291 340
Size: 438 2329 184 1485
Fragments arranged by size:
2329 1485 438 184
MscI TGG'CCA
Cuts at: 1112 1417 2197 1112
Size: 305 780 3351
Fragments arranged by size:
3351 780 305
MslI CAynn'nnrTG
Cuts at: 1360 2425 2552 1360
Size: 1065 127 3244
Fragments arranged by size:
3244 1065 127
MspA1I CmG'CkG
Cuts at: 322 415 570 692 1547 1861 2221 3234 3479
Size: 93 155 122 855 314 360 1013 245
Cuts at: 3479 322
Size: 1279
Fragments arranged by size:
1279 1013 855 360 314 245 155 122
93
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 4436
NarI GG'CG_CC
Cuts at: 1867 2115 1867
Size: 248 4188
NcoI C'CATG_G
Cuts at: 107 107
Size: 4436
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 4436
NheI G'CTAG_C
Cuts at: 1389 1389
Size: 4436
NspI r_CATG'y
Cuts at: 1489 1989 2896 1489
Size: 500 907 3029
Fragments arranged by size:
3029 907 500
PciI A'CATG_T
Cuts at: 2892 2892
Size: 4436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4436
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PmlI CAC'GTG
Cuts at: 471 471
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4404
PpuMI rG'GwC_Cy
Cuts at: 1172 1172
Size: 4436
PstI C_TGCA'G
Cuts at: 254 839 1483 2168 254
Size: 585 644 685 2522
Fragments arranged by size:
2522 685 644 585
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 415 1861 2221 415
Size: 1446 360 2630
Fragments arranged by size:
2630 1446 360
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 360 1450 360
Size: 1090 3346
SacII CC_GC'GG
Cuts at: 571 571
Size: 4436
SalI G'TCGA_C
Cuts at: 1473 1473
Size: 4436
SanDI GG'GwC_CC
Cuts at: 1172 1172
Size: 4436
SapI GCTCTTCn'nnn_
Cuts at: 2459 2669 2459
Size: 210 4226
SbfI CC_TGCA'GG
Cuts at: 1483 1483
Size: 4436
ScaI AGT'ACT
Cuts at: 3953 3953
Size: 4436
SfcI C'TryA_G
Cuts at: 33 250 696 835 1479 2164 3157 3348 33
Size: 217 446 139 644 685 993 191 1121
Fragments arranged by size:
1121 993 685 644 446 217 191 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 596 596
Size: 4436
SfoI GGC'GCC
Cuts at: 1868 2116 1868
Size: 248 4188
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 769 1010 1458 769
Size: 241 448 3747
Fragments arranged by size:
3747 448 241
SmlI C'TyrA_G
Cuts at: 1591 2506 2998 3260 1591
Size: 915 492 262 2767
Fragments arranged by size:
2767 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 1489 1989 1489
Size: 500 3936
StyI C'CwwG_G
Cuts at: 107 533 1570 1924 107
Size: 426 1037 354 2619
Fragments arranged by size:
2619 1037 426 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 669 777 819 1204 2019 2152 2611 2820 669
Size: 108 42 385 815 133 459 209 2285
Fragments arranged by size:
2285 815 459 385 209 133 108 42
TatI w'GTAC_w
Cuts at: 1286 3951 1286
Size: 2665 1771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 603 635 3953 3985 603
Size: 32 3318 32 1054
Fragments arranged by size:
3318 1054 32 32
Tth111I GACn'n_nGTC
Cuts at: 1153 2233 1153
Size: 1080 3356
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 976 1165 1425 1931 976
Size: 189 260 506 3481
Fragments arranged by size:
3481 506 260 189
XmaI C'CCGG_G
Cuts at: 767 1008 1456 767
Size: 241 448 3747
Fragments arranged by size:
3747 448 241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BsmI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspOMI PspXI PsrI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI