Restriction map for
pF1KE2654
(Circular) (Six-base) MAPSORT of: pF1KE2654.seq Check: 4571 from: 1 to: 3950
pF1KE2654 3950 bp
With 182 enzymes: SgfI *
December 1, 2016 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 504 504
Size: 3950
AccI GT'mk_AC
Cuts at: 299 988 299
Size: 689 3261
Acc65I G'GTAC_C
Cuts at: 966 966
Size: 3950
AclI AA'CG_TT
Cuts at: 944 3790 944
Size: 2846 1104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1775 2207 2954 3222 886
Size: 889 432 747 268 1614
Fragments arranged by size:
1614 889 747 432 268
AflIII A'CryG_T
Cuts at: 221 528 1302 2406 3383 221
Size: 307 774 1104 977 788
Fragments arranged by size:
1104 977 788 774 307
AgeI A'CCGG_T
Cuts at: 2338 2338
Size: 3950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 904
Size: 3950
AlwNI CAG_nnn'CTG
Cuts at: 1255 2822 1255
Size: 1567 2383
ApaLI G'TGCA_C
Cuts at: 896 2720 896
Size: 1824 2126
ApoI r'AATT_y
Cuts at: 954 3303 3559 954
Size: 2349 256 1345
Fragments arranged by size:
2349 1345 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3950
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
AvaI C'yCGr_G
Cuts at: 585 970 585
Size: 385 3565
BamHI G'GATC_C
Cuts at: 161 975 161
Size: 814 3136
BanI G'GyrC_C
Cuts at: 826 966 1380 1628 1663 826
Size: 140 414 248 35 3113
Fragments arranged by size:
3113 414 248 140 35
BanII G_rGCy'C
Cuts at: 601 918 964 1994 601
Size: 317 46 1030 2557
Fragments arranged by size:
2557 1030 317 46
BbeI G_GCGC'C
Cuts at: 1384 1632 1384
Size: 248 3702
BbsI GAAGACnn'nnnn_
Cuts at: 525 525
Size: 3950
BciVI GTATCCnnnnn_n'
Cuts at: 1848 2609 3950 1848
Size: 761 1341 1848
Fragments arranged by size:
1848 1341 761
BclI T'GATC_A
Cuts at: 1470 1470
Size: 3950
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3950
BglII A'GATC_T
Cuts at: 1465 3502 1465
Size: 2037 1913
BlpI GC'TnA_GC
Cuts at: 364 1062 364
Size: 698 3252
Bme1580I G_kGCm'C
Cuts at: 900 1575 1668 2724 900
Size: 675 93 1056 2126
Fragments arranged by size:
2126 1056 675 93
BmrI ACTGGGnnnn_n'
Cuts at: 753 790 1162 1243 1578 3722 753
Size: 37 372 81 335 2144 981
Fragments arranged by size:
2144 981 372 335 81 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 3950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 576 902 576
Size: 326 3624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 2005 2497 2795 1126
Size: 879 492 298 2281
Fragments arranged by size:
2281 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 747 3654 747
Size: 2907 1043
BsaAI yAC'GTr
Cuts at: 222 1303 222
Size: 1081 2869
BsaBI GATnn'nnATC
Cuts at: 6 1464 3501 6
Size: 1458 2037 455
Fragments arranged by size:
2037 1458 455
BsaHI Gr'CG_yC
Cuts at: 1381 1629 2331 3827 1381
Size: 248 702 1496 1504
Fragments arranged by size:
1504 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1139 1660 2338 2612 2759 3170 1139
Size: 521 678 274 147 411 1919
Fragments arranged by size:
1919 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1375 2133 2710 3206 1375
Size: 758 577 496 2119
Fragments arranged by size:
2119 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 3950
BsiEI CG_ry'CG
Cuts at: 104 1492 1538 2746 104
Size: 1388 46 1208 1308
Fragments arranged by size:
1388 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 370 900 918 964 1742 1932 2724 370
Size: 530 18 46 778 190 792 1596
Fragments arranged by size:
1596 792 778 530 190 46 18
Bsp1286I G_dGCh'C
Cuts at: 370 601 900 918 964 1575 1668 1742 1932
Size: 231 299 18 46 611 93 74 190
Cuts at: 1932 1994 2724 370
Size: 62 730 1596
Fragments arranged by size:
1596 730 611 299 231 190 93 74
62 46 18
BspEI T'CCGG_A
Cuts at: 3170 3170
Size: 3950
BspHI T'CATG_A
Cuts at: 290 3126 290
Size: 2836 1114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 504 1000 1214 1516 1897 504
Size: 496 214 302 381 2557
Fragments arranged by size:
2557 496 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2242 2296 3780 3945 50
Size: 2192 54 1484 165 55
Fragments arranged by size:
2192 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1862 1862
Size: 3950
BsrFI r'CCGG_y
Cuts at: 1948 2129 2338 1948
Size: 181 209 3560
Fragments arranged by size:
3560 209 181
BssSI C'ACGA_G
Cuts at: 2221 2579 2221
Size: 358 3592
BstAPI GCAn_nnn'nTGC
Cuts at: 1327 1327
Size: 3950
BstBI TT'CG_AA
Cuts at: 2311 2311
Size: 3950
BstEII G'GTnAC_C
Cuts at: 516 516
Size: 3950
BstXI CCAn_nnnn'nTGG
Cuts at: 283 283
Size: 3950
BstYI r'GATC_y
Cuts at: 1 153 161 975 1442 1465 1800 2046 3058
Size: 152 8 814 467 23 335 246 1012
Cuts at: 3058 3144 3156 3502 1
Size: 86 12 346 449
Fragments arranged by size:
1012 814 467 449 346 335 246 152
86 23 12 8
Bsu36I CC'TnA_GG
Cuts at: 413 413
Size: 3950
BtgI C'CryG_G
Cuts at: 107 282 107
Size: 175 3775
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1900 2081 2244 1900
Size: 181 163 3606
Fragments arranged by size:
3606 181 163
BtsI GCAGTG_nn'
Cuts at: 745 1193 745
Size: 448 3502
DraI TTT'AAA
Cuts at: 949 949
Size: 3950
DraIII CAC_nnn'GTG
Cuts at: 546 546
Size: 3950
DrdI GACnn_nn'nnGTC
Cuts at: 1656 2514 1656
Size: 858 3092
EaeI y'GGCC_r
Cuts at: 1535 1709 2100 2127 3472 1535
Size: 174 391 27 1345 2013
Fragments arranged by size:
2013 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1535 1535
Size: 3950
EarI CTCTTCn'nnn_
Cuts at: 451 457 627 1973 2183 451
Size: 6 170 1346 210 2218
Fragments arranged by size:
2218 1346 210 170 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2158 2468 2614 3760 2158
Size: 310 146 1146 2348
Fragments arranged by size:
2348 1146 310 146
EcoICRI GAG'CTC
Cuts at: 916 962 916
Size: 46 3904
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 576 886 902 1775 2207 2954 3222 576
Size: 310 16 873 432 747 268 1304
Fragments arranged by size:
1304 873 747 432 310 268 16
EcoO109I rG'GnC_Cy
Cuts at: 185 1089 185
Size: 904 3046
EcoRI G'AATT_C
Cuts at: 954 954
Size: 3950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3950
FspI TGC'GCA
Cuts at: 1731 1731
Size: 3950
HaeII r_GCGC'y
Cuts at: 384 1384 1632 2654 3633 384
Size: 1000 248 1022 979 701
Fragments arranged by size:
1022 1000 979 701 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1792 1822 1824 1854 1792
Size: 30 2 30 3888
Fragments arranged by size:
3888 30 30 2
HincII GTy'rAC
Cuts at: 989 989
Size: 3950
KasI G'GCGC_C
Cuts at: 1380 1628 1380
Size: 248 3702
KpnI G_GTAC'C
Cuts at: 970 970
Size: 3950
MluI A'CGCG_T
Cuts at: 3383 3383
Size: 3950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 428 875 2621 2805 428
Size: 447 1746 184 1573
Fragments arranged by size:
1746 1573 447 184
MscI TGG'CCA
Cuts at: 1711 1711
Size: 3950
MslI CAynn'nnrTG
Cuts at: 817 1939 2066 817
Size: 1122 127 2701
Fragments arranged by size:
2701 1122 127
MspA1I CmG'CkG
Cuts at: 723 779 1061 1375 1735 2748 2993 723
Size: 56 282 314 360 1013 245 1680
Fragments arranged by size:
1680 1013 360 314 282 245 56
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 3950
NarI GG'CG_CC
Cuts at: 1381 1629 1381
Size: 248 3702
NcoI C'CATG_G
Cuts at: 107 107
Size: 3950
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 3950
NspI r_CATG'y
Cuts at: 532 634 1003 1503 2410 532
Size: 102 369 500 907 2072
Fragments arranged by size:
2072 907 500 369 102
PciI A'CATG_T
Cuts at: 528 2406 528
Size: 1878 2072
PflMI CCAn_nnn'nTGG
Cuts at: 857 857
Size: 3950
PmeI GTTT'AAAC
Cuts at: 949 949
Size: 3950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3918
PpuMI rG'GwC_Cy
Cuts at: 185 185
Size: 3950
PstI C_TGCA'G
Cuts at: 997 1682 997
Size: 685 3265
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3950
PvuII CAG'CTG
Cuts at: 723 779 1375 1735 723
Size: 56 596 360 2938
Fragments arranged by size:
2938 596 360 56
RsrII CG'GwC_CG
Cuts at: 2145 2145
Size: 3950
SacI G_AGCT'C
Cuts at: 918 964 918
Size: 46 3904
SalI G'TCGA_C
Cuts at: 987 987
Size: 3950
SanDI GG'GwC_CC
Cuts at: 185 185
Size: 3950
SapI GCTCTTCn'nnn_
Cuts at: 451 1973 2183 451
Size: 1522 210 2218
Fragments arranged by size:
2218 1522 210
SbfI CC_TGCA'GG
Cuts at: 997 997
Size: 3950
ScaI AGT'ACT
Cuts at: 763 3467 763
Size: 2704 1246
SfcI C'TryA_G
Cuts at: 33 993 1678 2671 2862 33
Size: 960 685 993 191 1121
Fragments arranged by size:
1121 993 960 685 191
SfoI GGC'GCC
Cuts at: 1382 1630 1382
Size: 248 3702
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
SmaI CCC'GGG
Cuts at: 587 972 587
Size: 385 3565
SmlI C'TyrA_G
Cuts at: 1105 2020 2512 2774 1105
Size: 915 492 262 2281
Fragments arranged by size:
2281 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3950
SphI G_CATG'C
Cuts at: 1003 1503 1003
Size: 500 3450
StyI C'CwwG_G
Cuts at: 107 875 929 1084 1438 107
Size: 768 54 155 354 2619
Fragments arranged by size:
2619 768 354 155 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1533 1666 2125 2334 1533
Size: 133 459 209 3149
Fragments arranged by size:
3149 459 209 133
TatI w'GTAC_w
Cuts at: 224 647 761 3465 224
Size: 423 114 2704 709
Fragments arranged by size:
2704 709 423 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3467 3499 3467
Size: 32 3918
Tth111I GACn'n_nGTC
Cuts at: 1747 1747
Size: 3950
XbaI T'CTAG_A
Cuts at: 981 981
Size: 3950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1445 1445
Size: 3950
XmaI C'CCGG_G
Cuts at: 585 970 585
Size: 385 3565
XmnI GAAnn'nnTTC
Cuts at: 271 271
Size: 3950
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI