Restriction map for
pF1KE2648
(Circular) (Six-base) MAPSORT of: pF1KE2648.seq Check: 211 from: 1 to: 5513
pF1KE2648 5513 bp
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AccI GT'mk_AC
Cuts at: 577 2378 2551 577
Size: 1801 173 3539
Fragments arranged by size:
3539 1801 173
Acc65I G'GTAC_C
Cuts at: 900 2529 900
Size: 1629 3884
AclI AA'CG_TT
Cuts at: 5353 5353
Size: 5513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1702 1717 2433 3338 3770 4517 4785 1511
Size: 191 15 716 905 432 747 268 2239
Fragments arranged by size:
2239 905 747 716 432 268 191 15
AflIII A'CryG_T
Cuts at: 423 2865 3969 4946 423
Size: 2442 1104 977 990
Fragments arranged by size:
2442 1104 990 977
AgeI A'CCGG_T
Cuts at: 3901 3901
Size: 5513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 766 1693 766
Size: 927 4586
AlwNI CAG_nnn'CTG
Cuts at: 183 693 1285 1944 2818 4385 183
Size: 510 592 659 874 1567 1311
Fragments arranged by size:
1567 1311 874 659 592 510
ApaLI G'TGCA_C
Cuts at: 4283 4283
Size: 5513
ApoI r'AATT_y
Cuts at: 306 1267 1309 1430 1949 2014 2443 2517 4866
Size: 961 42 121 519 65 429 74 2349
Cuts at: 4866 5122 306
Size: 256 697
Fragments arranged by size:
2349 961 697 519 429 256 121 74
65 42
AseI AT'TA_AT
Cuts at: 20 2278 2332 20
Size: 2258 54 3201
Fragments arranged by size:
3201 2258 54
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
AvaI C'yCGr_G
Cuts at: 141 2533 141
Size: 2392 3121
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 383 416 383
Size: 33 5480
BamHI G'GATC_C
Cuts at: 2131 2538 2131
Size: 407 5106
BanI G'GyrC_C
Cuts at: 900 2529 2943 3191 3226 900
Size: 1629 414 248 35 3187
Fragments arranged by size:
3187 1629 414 248 35
BanII G_rGCy'C
Cuts at: 1043 1244 2527 3557 1043
Size: 201 1283 1030 2999
Fragments arranged by size:
2999 1283 1030 201
BbeI G_GCGC'C
Cuts at: 2947 3195 2947
Size: 248 5265
BbsI GAAGACnn'nnnn_
Cuts at: 341 341
Size: 5513
BbvCI CC'TCA_GC
Cuts at: 2172 2172
Size: 5513
BciVI GTATCCnnnnn_n'
Cuts at: 950 968 974 3411 4172 5513 950
Size: 18 6 2437 761 1341 950
Fragments arranged by size:
2437 1341 950 761 18 6
BclI T'GATC_A
Cuts at: 3033 3033
Size: 5513
BfrBI ATG'CAT
Cuts at: 1514 1514
Size: 5513
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5513
BglII A'GATC_T
Cuts at: 1400 3028 5065 1400
Size: 1628 2037 1848
Fragments arranged by size:
2037 1848 1628
BlpI GC'TnA_GC
Cuts at: 2625 2625
Size: 5513
Bme1580I G_kGCm'C
Cuts at: 3138 3231 4287 3138
Size: 93 1056 4364
Fragments arranged by size:
4364 1056 93
BmgBI CAC'GTC
Cuts at: 1303 1303
Size: 5513
BmrI ACTGGGnnnn_n'
Cuts at: 193 1395 1609 1878 2725 2806 3141 5285 193
Size: 1202 214 269 847 81 335 2144 421
Fragments arranged by size:
2144 1202 847 421 335 269 214 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3880 3880
Size: 5513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 950 2375 620
Size: 330 1425 3758
Fragments arranged by size:
3758 1425 330
Bpu10I CC'TnA_GC
Cuts at: 2172 2172
Size: 5513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2689 3568 4060 4358 2689
Size: 879 492 298 3844
Fragments arranged by size:
3844 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1162 5217 1162
Size: 4055 1458
BsaAI yAC'GTr
Cuts at: 1217 2866 1217
Size: 1649 3864
BsaBI GATnn'nnATC
Cuts at: 6 1399 3027 5064 6
Size: 1393 1628 2037 455
Fragments arranged by size:
2037 1628 1393 455
BsaHI Gr'CG_yC
Cuts at: 2944 3192 3894 5390 2944
Size: 248 702 1496 3067
Fragments arranged by size:
3067 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2702 3223 3901 4175 4322 4733 2702
Size: 521 678 274 147 411 3482
Fragments arranged by size:
3482 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1231 1261 2026 2056 1231
Size: 30 765 30 4688
Fragments arranged by size:
4688 765 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1252 1252
Size: 5513
BseYI C'CCAG_C
Cuts at: 1644 2938 3696 4273 4769 1644
Size: 1294 758 577 496 2388
Fragments arranged by size:
2388 1294 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 3055 3101 4309 104
Size: 12 2939 46 1208 1308
Fragments arranged by size:
2939 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 1043 1447 2527 3305 3495 4287 1043
Size: 404 1080 778 190 792 2269
Fragments arranged by size:
2269 1080 792 778 404 190
BsmI GAATG_Cn'
Cuts at: 828 828
Size: 5513
BsmBI CGTCTCn'nnnn_
Cuts at: 1250 1250
Size: 5513
Bsp1286I G_dGCh'C
Cuts at: 1043 1244 1447 2527 3138 3231 3305 3495 3557
Size: 201 203 1080 611 93 74 190 62
Cuts at: 3557 4287 1043
Size: 730 2269
Fragments arranged by size:
2269 1080 730 611 203 201 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4733 4733
Size: 5513
BspHI T'CATG_A
Cuts at: 1529 4689 1529
Size: 3160 2353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 964 2364 2563 2777 3079 3460 964
Size: 1400 199 214 302 381 3017
Fragments arranged by size:
3017 1400 381 302 214 199
BsrBI CCG'CTC
Cuts at: 50 3805 3859 5343 5508 50
Size: 3755 54 1484 165 55
Fragments arranged by size:
3755 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 748 3425 748
Size: 2677 2836
BsrFI r'CCGG_y
Cuts at: 168 3511 3692 3901 168
Size: 3343 181 209 1780
Fragments arranged by size:
3343 1780 209 181
BsrGI T'GTAC_A
Cuts at: 1475 1543 1475
Size: 68 5445
BssSI C'ACGA_G
Cuts at: 3784 4142 3784
Size: 358 5155
BstAPI GCAn_nnn'nTGC
Cuts at: 2890 2890
Size: 5513
BstBI TT'CG_AA
Cuts at: 3874 3874
Size: 5513
BstEII G'GTnAC_C
Cuts at: 851 851
Size: 5513
BstXI CCAn_nnnn'nTGG
Cuts at: 1116 1116
Size: 5513
BstYI r'GATC_y
Cuts at: 1 1400 2131 2538 3005 3028 3363 3609 4621
Size: 1399 731 407 467 23 335 246 1012
Cuts at: 4621 4707 4719 5065 1
Size: 86 12 346 449
Fragments arranged by size:
1399 1012 731 467 449 407 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 578 578
Size: 5513
Bsu36I CC'TnA_GG
Cuts at: 968 968
Size: 5513
BtgI C'CryG_G
Cuts at: 908 2135 908
Size: 1227 4286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 822 3463 3644 3807 822
Size: 2641 181 163 2528
Fragments arranged by size:
2641 2528 181 163
BtsI GCAGTG_nn'
Cuts at: 2756 2756
Size: 5513
DraI TTT'AAA
Cuts at: 527 1062 2124 2512 527
Size: 535 1062 388 3528
Fragments arranged by size:
3528 1062 535 388
DrdI GACnn_nn'nnGTC
Cuts at: 3219 4077 3219
Size: 858 4655
EaeI y'GGCC_r
Cuts at: 113 3098 3272 3663 3690 5035 113
Size: 2985 174 391 27 1345 591
Fragments arranged by size:
2985 1345 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 3098 113
Size: 2985 2528
EarI CTCTTCn'nnn_
Cuts at: 809 1811 2404 3536 3746 809
Size: 1002 593 1132 210 2576
Fragments arranged by size:
2576 1132 1002 593 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 3721 4031 4177 5323 187
Size: 3534 310 146 1146 377
Fragments arranged by size:
3534 1146 377 310 146
EcoICRI GAG'CTC
Cuts at: 1041 2525 1041
Size: 1484 4029
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 950 1511 1702 1717 2375 2433 3338 3770
Size: 330 561 191 15 658 58 905 432
Cuts at: 3770 4517 4785 620
Size: 747 268 1348
Fragments arranged by size:
1348 905 747 658 561 432 330 268
191 58 15
EcoNI CCTnn'n_nnAGG
Cuts at: 971 971
Size: 5513
EcoO109I rG'GnC_Cy
Cuts at: 1611 2652 1611
Size: 1041 4472
EcoRI G'AATT_C
Cuts at: 2517 2517
Size: 5513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3532 3532
Size: 5513
FspI TGC'GCA
Cuts at: 3294 3294
Size: 5513
HaeII r_GCGC'y
Cuts at: 2947 3195 4217 5196 2947
Size: 248 1022 979 3264
Fragments arranged by size:
3264 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1192 1224 2403 2435 3355 3385 3387 3417 1192
Size: 32 1179 32 920 30 2 30 3288
Fragments arranged by size:
3288 1179 920 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2552 2552
Size: 5513
HindIII A'AGCT_T
Cuts at: 2116 2116
Size: 5513
KasI G'GCGC_C
Cuts at: 2943 3191 2943
Size: 248 5265
KpnI G_GTAC'C
Cuts at: 904 2533 904
Size: 1629 3884
MluI A'CGCG_T
Cuts at: 4946 4946
Size: 5513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2340 4184 4368 2340
Size: 1844 184 3485
Fragments arranged by size:
3485 1844 184
MscI TGG'CCA
Cuts at: 3274 3274
Size: 5513
MslI CAynn'nnrTG
Cuts at: 268 1324 1888 3502 3629 268
Size: 1056 564 1614 127 2152
Fragments arranged by size:
2152 1614 1056 564 127
MspA1I CmG'CkG
Cuts at: 693 1473 1842 2008 2624 2938 3298 4311 4556
Size: 780 369 166 616 314 360 1013 245
Cuts at: 4556 693
Size: 1650
Fragments arranged by size:
1650 1013 780 616 369 360 314 245
166
NaeI GCC'GGC
Cuts at: 170 3694 170
Size: 3524 1989
NarI GG'CG_CC
Cuts at: 2944 3192 2944
Size: 248 5265
NcoI C'CATG_G
Cuts at: 908 908
Size: 5513
NdeI CA'TA_TG
Cuts at: 546 588 546
Size: 42 5471
NgoMIV G'CCGG_C
Cuts at: 168 3692 168
Size: 3524 1989
NsiI A_TGCA'T
Cuts at: 1516 1516
Size: 5513
NspI r_CATG'y
Cuts at: 427 2566 3066 3973 427
Size: 2139 500 907 1967
Fragments arranged by size:
2139 1967 907 500
PciI A'CATG_T
Cuts at: 423 3969 423
Size: 3546 1967
PflMI CCAn_nnn'nTGG
Cuts at: 183 183
Size: 5513
PmeI GTTT'AAAC
Cuts at: 2512 2512
Size: 5513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 4652
Size: 32 5481
PpuMI rG'GwC_Cy
Cuts at: 1611 1611
Size: 5513
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1742 1774 1742
Size: 32 5481
PstI C_TGCA'G
Cuts at: 2560 3245 2560
Size: 685 4828
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5513
PvuII CAG'CTG
Cuts at: 693 1473 1842 2008 2938 3298 693
Size: 780 369 166 930 360 2908
Fragments arranged by size:
2908 930 780 369 360 166
RsrII CG'GwC_CG
Cuts at: 3708 3708
Size: 5513
SacI G_AGCT'C
Cuts at: 1043 2527 1043
Size: 1484 4029
SalI G'TCGA_C
Cuts at: 2550 2550
Size: 5513
SanDI GG'GwC_CC
Cuts at: 1611 1611
Size: 5513
SapI GCTCTTCn'nnn_
Cuts at: 1811 2404 3536 3746 1811
Size: 593 1132 210 3578
Fragments arranged by size:
3578 1132 593 210
SbfI CC_TGCA'GG
Cuts at: 2560 2560
Size: 5513
ScaI AGT'ACT
Cuts at: 5030 5030
Size: 5513
SfcI C'TryA_G
Cuts at: 33 1175 2427 2556 3241 4234 4425 33
Size: 1142 1252 129 685 993 191 1121
Fragments arranged by size:
1252 1142 1121 993 685 191 129
SfoI GGC'GCC
Cuts at: 2945 3193 2945
Size: 248 5265
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
SmaI CCC'GGG
Cuts at: 143 2535 143
Size: 2392 3121
SmlI C'TyrA_G
Cuts at: 2668 3583 4075 4337 2668
Size: 915 492 262 3844
Fragments arranged by size:
3844 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5513
SphI G_CATG'C
Cuts at: 2566 3066 2566
Size: 500 5013
SspI AAT'ATT
Cuts at: 1654 1654
Size: 5513
StyI C'CwwG_G
Cuts at: 681 908 1606 2193 2647 3001 681
Size: 227 698 587 454 354 3193
Fragments arranged by size:
3193 698 587 454 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1805 3096 3229 3688 3897 1805
Size: 1291 133 459 209 3421
Fragments arranged by size:
3421 1291 459 209 133
TatI w'GTAC_w
Cuts at: 1475 1543 5028 1475
Size: 68 3485 1960
Fragments arranged by size:
3485 1960 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5030 5062 5030
Size: 32 5481
Tth111I GACn'n_nGTC
Cuts at: 652 3310 652
Size: 2658 2855
XbaI T'CTAG_A
Cuts at: 1252 2544 1252
Size: 1292 4221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 209 327 377 465 1782 3008 209
Size: 118 50 88 1317 1226 2714
Fragments arranged by size:
2714 1317 1226 118 88 50
XmaI C'CCGG_G
Cuts at: 141 2533 141
Size: 2392 3121
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BcgI BmtI BsgI BsiWI BssHII ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI