Restriction map for
pF1KE2641
(Circular) (Six-base) MAPSORT of: pF1KE2641.seq Check: 4172 from: 1 to: 4430
pF1KE2641 4430 bp
With 182 enzymes: SgfI *
October 5, 2016 15:26 ..
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 570 1446 570
Size: 876 3554
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 781 919 1439 2255 2687 3434 3702 560
Size: 221 138 520 816 432 747 268 1288
Fragments arranged by size:
1288 816 747 520 432 268 221 138
AfeI AGC'GCT
Cuts at: 916 916
Size: 4430
AflIII A'CryG_T
Cuts at: 1020 1275 1782 2886 3863 1020
Size: 255 507 1104 977 1587
Fragments arranged by size:
1587 1104 977 507 255
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 1735 3302 1735
Size: 1567 2863
ApaLI G'TGCA_C
Cuts at: 3200 3200
Size: 4430
ApoI r'AATT_y
Cuts at: 448 1095 1434 3783 4039 448
Size: 647 339 2349 256 839
Fragments arranged by size:
2349 839 647 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 1450 1450
Size: 4430
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 782 815 782
Size: 33 4397
BamHI G'GATC_C
Cuts at: 1455 1455
Size: 4430
BanI G'GyrC_C
Cuts at: 570 1039 1446 1860 2108 2143 570
Size: 469 407 414 248 35 2857
Fragments arranged by size:
2857 469 414 407 248 35
BanII G_rGCy'C
Cuts at: 1444 2474 1444
Size: 1030 3400
BbeI G_GCGC'C
Cuts at: 1864 2112 1864
Size: 248 4182
BbsI GAAGACnn'nnnn_
Cuts at: 819 819
Size: 4430
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 110 702 1950 110
Size: 592 1248 2590
Fragments arranged by size:
2590 1248 592
BglII A'GATC_T
Cuts at: 1945 3982 1945
Size: 2037 2393
BlpI GC'TnA_GC
Cuts at: 1542 1542
Size: 4430
Bme1580I G_kGCm'C
Cuts at: 1042 2055 2148 3204 1042
Size: 1013 93 1056 2268
Fragments arranged by size:
2268 1056 1013 93
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4202 1642
Size: 81 335 2144 1870
Fragments arranged by size:
2144 1870 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 153 832 2797 153
Size: 679 1965 1786
Fragments arranged by size:
1965 1786 679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 986 1133 1309 779
Size: 207 147 176 3900
Fragments arranged by size:
3900 207 176 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 895 1606 2485 2977 3275 127
Size: 768 711 879 492 298 1282
Fragments arranged by size:
1282 879 768 711 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 4134 187
Size: 3947 483
BsaAI yAC'GTr
Cuts at: 1783 1783
Size: 4430
BsaBI GATnn'nnATC
Cuts at: 6 316 1944 3981 6
Size: 310 1628 2037 455
Fragments arranged by size:
2037 1628 455 310
BsaHI Gr'CG_yC
Cuts at: 1861 2109 2811 4307 1861
Size: 248 702 1496 1984
Fragments arranged by size:
1984 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1619 2140 2818 3092 3239 3650 1619
Size: 521 678 274 147 411 2399
Fragments arranged by size:
2399 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 984 1014 1131 1161 1307 1337 984
Size: 30 117 30 146 30 4077
Fragments arranged by size:
4077 146 117 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 142
Size: 4430
BseYI C'CCAG_C
Cuts at: 1855 2613 3190 3686 1855
Size: 758 577 496 2599
Fragments arranged by size:
2599 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1001 1001
Size: 4430
BsiEI CG_ry'CG
Cuts at: 104 1972 2018 3226 104
Size: 1868 46 1208 1308
Fragments arranged by size:
1868 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 524 1444 2222 2412 3204 524
Size: 920 778 190 792 1750
Fragments arranged by size:
1750 920 792 778 190
BsmI GAATG_Cn'
Cuts at: 543 1400 543
Size: 857 3573
Bsp1286I G_dGCh'C
Cuts at: 524 1042 1444 2055 2148 2222 2412 2474 3204
Size: 518 402 611 93 74 190 62 730
Cuts at: 3204 524
Size: 1750
Fragments arranged by size:
1750 730 611 518 402 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3650 3650
Size: 4430
BspHI T'CATG_A
Cuts at: 459 669 3606 459
Size: 210 2937 1283
Fragments arranged by size:
2937 1283 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 1480 1694 1996 2377 164
Size: 1316 214 302 381 2217
Fragments arranged by size:
2217 1316 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2722 2776 4260 4425 50
Size: 2672 54 1484 165 55
Fragments arranged by size:
2672 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4430
BsrFI r'CCGG_y
Cuts at: 860 2428 2609 2818 860
Size: 1568 181 209 2472
Fragments arranged by size:
2472 1568 209 181
BssSI C'ACGA_G
Cuts at: 2701 3059 2701
Size: 358 4072
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4430
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 177 177
Size: 4430
BstYI r'GATC_y
Cuts at: 1 480 1455 1922 1945 2280 2526 3538 3624
Size: 479 975 467 23 335 246 1012 86
Cuts at: 3624 3636 3982 1
Size: 12 346 449
Fragments arranged by size:
1012 975 479 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 196 549 196
Size: 353 4077
BtgI C'CryG_G
Cuts at: 1237 1237
Size: 4430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2380 2561 2724 2380
Size: 181 163 4086
Fragments arranged by size:
4086 181 163
BtsI GCAGTG_nn'
Cuts at: 1673 1673
Size: 4430
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4430
DrdI GACnn_nn'nnGTC
Cuts at: 2136 2994 2136
Size: 858 3572
EaeI y'GGCC_r
Cuts at: 354 2015 2189 2580 2607 3952 354
Size: 1661 174 391 27 1345 832
Fragments arranged by size:
1661 1345 832 391 174 27
EagI C'GGCC_G
Cuts at: 2015 2015
Size: 4430
EarI CTCTTCn'nnn_
Cuts at: 1331 2453 2663 1331
Size: 1122 210 3098
Fragments arranged by size:
3098 1122 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2638 2948 3094 4240 2638
Size: 310 146 1146 2828
Fragments arranged by size:
2828 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 779 781 919 986 1133 1309 1439 2255
Size: 219 2 138 67 147 176 130 816
Cuts at: 2255 2687 3434 3702 560
Size: 432 747 268 1288
Fragments arranged by size:
1288 816 747 432 268 219 176 147
138 130 67 2
EcoNI CCTnn'n_nnAGG
Cuts at: 578 806 578
Size: 228 4202
EcoO109I rG'GnC_Cy
Cuts at: 1404 1569 1404
Size: 165 4265
EcoRI G'AATT_C
Cuts at: 448 1095 1434 448
Size: 647 339 3444
Fragments arranged by size:
3444 647 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 299 2449 299
Size: 2150 2280
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 918 1864 2112 3134 4113 918
Size: 946 248 1022 979 1235
Fragments arranged by size:
1235 1022 979 946 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 165 197 391 423 595 627 984 1016 2272
Size: 32 194 32 172 32 357 32 1256
Cuts at: 2272 2302 2304 2334 165
Size: 30 2 30 2261
Fragments arranged by size:
2261 1256 357 194 172 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1281 1469 1127
Size: 154 188 4088
Fragments arranged by size:
4088 188 154
HindIII A'AGCT_T
Cuts at: 664 664
Size: 4430
KasI G'GCGC_C
Cuts at: 1860 2108 1860
Size: 248 4182
KpnI G_GTAC'C
Cuts at: 574 1450 574
Size: 876 3554
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3285 3101
Size: 184 4246
MscI TGG'CCA
Cuts at: 356 2191 356
Size: 1835 2595
MslI CAynn'nnrTG
Cuts at: 2419 2546 2419
Size: 127 4303
MspA1I CmG'CkG
Cuts at: 958 1541 1855 2215 3228 3473 958
Size: 583 314 360 1013 245 1915
Fragments arranged by size:
1915 1013 583 360 314 245
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 4430
NarI GG'CG_CC
Cuts at: 1861 2109 1861
Size: 248 4182
NcoI C'CATG_G
Cuts at: 1237 1237
Size: 4430
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 4430
NspI r_CATG'y
Cuts at: 1024 1279 1483 1983 2890 1024
Size: 255 204 500 907 2564
Fragments arranged by size:
2564 907 500 255 204
PciI A'CATG_T
Cuts at: 1020 1275 2886 1020
Size: 255 1611 2564
Fragments arranged by size:
2564 1611 255
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4398
PshAI GACnn'nnGTC
Cuts at: 1150 1150
Size: 4430
PstI C_TGCA'G
Cuts at: 161 933 1477 2162 161
Size: 772 544 685 2429
Fragments arranged by size:
2429 772 685 544
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 958 1855 2215 958
Size: 897 360 3173
Fragments arranged by size:
3173 897 360
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 1331 2453 2663 1331
Size: 1122 210 3098
Fragments arranged by size:
3098 1122 210
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4430
ScaI AGT'ACT
Cuts at: 3947 3947
Size: 4430
SfcI C'TryA_G
Cuts at: 33 157 929 1473 2158 3151 3342 33
Size: 124 772 544 685 993 191 1121
Fragments arranged by size:
1121 993 772 685 544 191 124
SfoI GGC'GCC
Cuts at: 1862 2110 1862
Size: 248 4182
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 1452 1452
Size: 4430
SmlI C'TyrA_G
Cuts at: 142 910 1585 2500 2992 3254 142
Size: 768 675 915 492 262 1318
Fragments arranged by size:
1318 915 768 675 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3930
StyI C'CwwG_G
Cuts at: 890 1237 1564 1918 890
Size: 347 327 354 3402
Fragments arranged by size:
3402 354 347 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2013 2146 2605 2814 2013
Size: 133 459 209 3629
Fragments arranged by size:
3629 459 209 133
TatI w'GTAC_w
Cuts at: 146 3945 146
Size: 3799 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 4398
Tth111I GACn'n_nGTC
Cuts at: 1029 2227 1029
Size: 1198 3232
XbaI T'CTAG_A
Cuts at: 123 1461 123
Size: 1338 3092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1925 1925
Size: 4430
XmaI C'CCGG_G
Cuts at: 1450 1450
Size: 4430
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI