Restriction map for
pF1KE2639
(Circular) (Six-base) MAPSORT of: pF1KE2639.seq Check: 5157 from: 1 to: 5273
pF1KE2639 5273 bp
With 182 enzymes: SgfI *
September 8, 2016 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 206 1167 1461 206
Size: 961 294 4018
Fragments arranged by size:
4018 961 294
AccI GT'mk_AC
Cuts at: 554 764 1217 2255 2311 554
Size: 210 453 1038 56 3516
Fragments arranged by size:
3516 1038 453 210 56
Acc65I G'GTAC_C
Cuts at: 449 2289 449
Size: 1840 3433
AclI AA'CG_TT
Cuts at: 5113 5113
Size: 5273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 712 913 1501 1519 3098 3530 4277 4545
Size: 487 201 588 18 1579 432 747 268
Cuts at: 4545 225
Size: 953
Fragments arranged by size:
1579 953 747 588 487 432 268 201
18
AflIII A'CryG_T
Cuts at: 1700 2625 3729 4706 1700
Size: 925 1104 977 2267
Fragments arranged by size:
2267 1104 977 925
AgeI A'CCGG_T
Cuts at: 3661 3661
Size: 5273
AhdI GACnn_n'nnGTC
Cuts at: 1317 1317
Size: 5273
AleI CACnn'nnGTG
Cuts at: 409 409
Size: 5273
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1780 1812 1780
Size: 32 5241
AlwNI CAG_nnn'CTG
Cuts at: 368 1182 1497 1749 1869 2578 4145 368
Size: 814 315 252 120 709 1567 1496
Fragments arranged by size:
1567 1496 814 709 315 252 120
ApaLI G'TGCA_C
Cuts at: 963 4043 963
Size: 3080 2193
ApoI r'AATT_y
Cuts at: 1636 2277 4626 4882 1636
Size: 641 2349 256 2027
Fragments arranged by size:
2349 2027 641 256
AseI AT'TA_AT
Cuts at: 20 1979 20
Size: 1959 3314
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
AvaI C'yCGr_G
Cuts at: 326 2293 326
Size: 1967 3306
BamHI G'GATC_C
Cuts at: 632 986 1453 2298 632
Size: 354 467 845 3607
Fragments arranged by size:
3607 845 467 354
BanI G'GyrC_C
Cuts at: 167 449 536 853 2289 2703 2951 2986 167
Size: 282 87 317 1436 414 248 35 2454
Fragments arranged by size:
2454 1436 414 317 282 248 87 35
BanII G_rGCy'C
Cuts at: 151 2287 3317 151
Size: 2136 1030 2107
Fragments arranged by size:
2136 2107 1030
BbeI G_GCGC'C
Cuts at: 857 2707 2955 857
Size: 1850 248 3175
Fragments arranged by size:
3175 1850 248
BbsI GAAGACnn'nnnn_
Cuts at: 674 2106 674
Size: 1432 3841
BciVI GTATCCnnnnn_n'
Cuts at: 1092 3171 3932 5273 1092
Size: 2079 761 1341 1092
Fragments arranged by size:
2079 1341 1092 761
BclI T'GATC_A
Cuts at: 791 1001 1043 2793 791
Size: 210 42 1750 3271
Fragments arranged by size:
3271 1750 210 42
BglII A'GATC_T
Cuts at: 2788 4825 2788
Size: 2037 3236
BlpI GC'TnA_GC
Cuts at: 2385 2385
Size: 5273
Bme1580I G_kGCm'C
Cuts at: 541 967 1743 1902 2898 2991 4047 541
Size: 426 776 159 996 93 1056 1767
Fragments arranged by size:
1767 1056 996 776 426 159 93
BmrI ACTGGGnnnn_n'
Cuts at: 1604 1704 1737 1848 2485 2566 2901 5045 1604
Size: 100 33 111 637 81 335 2144 1832
Fragments arranged by size:
2144 1832 637 335 111 100 81 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3640 3640
Size: 5273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 135 403 852 1444 2179 127
Size: 8 268 449 592 735 3221
Fragments arranged by size:
3221 735 592 449 268 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3328 3820 4118 2449
Size: 879 492 298 3604
Fragments arranged by size:
3604 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 379 2155 4977 379
Size: 1776 2822 675
Fragments arranged by size:
2822 1776 675
BsaAI yAC'GTr
Cuts at: 2626 2626
Size: 5273
BsaBI GATnn'nnATC
Cuts at: 6 1891 2787 4824 6
Size: 1885 896 2037 455
Fragments arranged by size:
2037 1885 896 455
BsaHI Gr'CG_yC
Cuts at: 854 1187 1214 2704 2952 3654 5150 854
Size: 333 27 1490 248 702 1496 977
Fragments arranged by size:
1496 1490 977 702 333 248 27
BsaWI w'CCGG_w
Cuts at: 248 2462 2983 3661 3935 4082 4493 248
Size: 2214 521 678 274 147 411 1028
Fragments arranged by size:
2214 1028 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 457 487 1690 1720 1794 1824 1982 2012 457
Size: 30 1203 30 74 30 158 30 3718
Fragments arranged by size:
3718 1203 158 74 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 416 1544 193
Size: 223 1128 3922
Fragments arranged by size:
3922 1128 223
BseYI C'CCAG_C
Cuts at: 361 729 941 2698 3456 4033 4529 361
Size: 368 212 1757 758 577 496 1105
Fragments arranged by size:
1757 1105 758 577 496 368 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1738 697
Size: 1041 4232
BsiEI CG_ry'CG
Cuts at: 104 440 2815 2861 4069 104
Size: 336 2375 46 1208 1308
Fragments arranged by size:
2375 1308 1208 336 46
BsiHKAI G_wGCw'C
Cuts at: 151 747 821 967 2287 3065 3255 4047 151
Size: 596 74 146 1320 778 190 792 1377
Fragments arranged by size:
1377 1320 792 778 596 190 146 74
BsmI GAATG_Cn'
Cuts at: 1311 1311
Size: 5273
Bsp1286I G_dGCh'C
Cuts at: 151 541 747 821 967 1743 1902 2287 2898
Size: 390 206 74 146 776 159 385 611
Cuts at: 2898 2991 3065 3255 3317 4047 151
Size: 93 74 190 62 730 1377
Fragments arranged by size:
1377 776 730 611 390 385 206 190
159 146 93 74 74 62
BspEI T'CCGG_A
Cuts at: 4493 4493
Size: 5273
BspHI T'CATG_A
Cuts at: 590 4449 590
Size: 3859 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 1167 1461 2043 2323 2537 2839 3220 206
Size: 961 294 582 280 214 302 381 2259
Fragments arranged by size:
2259 961 582 381 302 294 280 214
BsrBI CCG'CTC
Cuts at: 50 1416 3565 3619 5103 5268 50
Size: 1366 2149 54 1484 165 55
Fragments arranged by size:
2149 1484 1366 165 55 54
BsrDI GCAATG_nn'
Cuts at: 807 943 3185 807
Size: 136 2242 2895
Fragments arranged by size:
2895 2242 136
BsrFI r'CCGG_y
Cuts at: 3271 3452 3661 3271
Size: 181 209 4883
Fragments arranged by size:
4883 209 181
BssSI C'ACGA_G
Cuts at: 1365 3544 3902 1365
Size: 2179 358 2736
Fragments arranged by size:
2736 2179 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2650 2650
Size: 5273
BstBI TT'CG_AA
Cuts at: 3634 3634
Size: 5273
BstXI CCAn_nnnn'nTGG
Cuts at: 647 1617 647
Size: 970 4303
BstYI r'GATC_y
Cuts at: 1 632 986 1149 1453 2192 2298 2765 2788
Size: 631 354 163 304 739 106 467 23
Cuts at: 2788 3123 3369 4381 4467 4479 4825 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 739 631 467 449 354 346 335
304 246 163 106 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1196 1196
Size: 5273
BtgI C'CryG_G
Cuts at: 107 440 1515 107
Size: 333 1075 3865
Fragments arranged by size:
3865 1075 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3223 3404 3567 3223
Size: 181 163 4929
Fragments arranged by size:
4929 181 163
BtsI GCAGTG_nn'
Cuts at: 904 2516 904
Size: 1612 3661
DraI TTT'AAA
Cuts at: 2272 2272
Size: 5273
DrdI GACnn_nn'nnGTC
Cuts at: 2979 3837 2979
Size: 858 4415
EaeI y'GGCC_r
Cuts at: 437 884 1020 2858 3032 3423 3450 4795 437
Size: 447 136 1838 174 391 27 1345 915
Fragments arranged by size:
1838 1345 915 447 391 174 136 27
EagI C'GGCC_G
Cuts at: 437 2858 437
Size: 2421 2852
EarI CTCTTCn'nnn_
Cuts at: 1904 2098 3296 3506 1904
Size: 194 1198 210 3671
Fragments arranged by size:
3671 1198 210 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 625 3481 3791 3937 5083 625
Size: 2856 310 146 1146 815
Fragments arranged by size:
2856 1146 815 310 146
EcoICRI GAG'CTC
Cuts at: 149 2285 149
Size: 2136 3137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 135 225 403 712 852 913 1444 1501
Size: 8 90 178 309 140 61 531 57
Cuts at: 1501 1519 2179 3098 3530 4277 4545 127
Size: 18 660 919 432 747 268 855
Fragments arranged by size:
919 855 747 660 531 432 309 268
178 140 90 61 57 18 8
EcoO109I rG'GnC_Cy
Cuts at: 1719 2412 1719
Size: 693 4580
EcoRI G'AATT_C
Cuts at: 2277 2277
Size: 5273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 3292 2134
Size: 1158 4115
FspI TGC'GCA
Cuts at: 3054 3054
Size: 5273
HaeII r_GCGC'y
Cuts at: 857 2707 2955 3977 4956 857
Size: 1850 248 1022 979 1174
Fragments arranged by size:
1850 1174 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 963 995 1303 1335 3115 3145 3147 3177 963
Size: 32 308 32 1780 30 2 30 3059
Fragments arranged by size:
3059 1780 308 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1874 2312 1874
Size: 438 4835
HindIII A'AGCT_T
Cuts at: 1351 1509 1351
Size: 158 5115
KasI G'GCGC_C
Cuts at: 853 2703 2951 853
Size: 1850 248 3175
Fragments arranged by size:
3175 1850 248
KpnI G_GTAC'C
Cuts at: 453 2293 453
Size: 1840 3433
MluI A'CGCG_T
Cuts at: 4706 4706
Size: 5273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3944 4128 3944
Size: 184 5089
MscI TGG'CCA
Cuts at: 886 1022 3034 886
Size: 136 2012 3125
Fragments arranged by size:
3125 2012 136
MslI CAynn'nnrTG
Cuts at: 283 409 970 2023 3262 3389 283
Size: 126 561 1053 1239 127 2167
Fragments arranged by size:
2167 1239 1053 561 127 126
MspA1I CmG'CkG
Cuts at: 303 2384 2698 3058 4071 4316 303
Size: 2081 314 360 1013 245 1260
Fragments arranged by size:
2081 1260 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3454 3454
Size: 5273
NarI GG'CG_CC
Cuts at: 854 2704 2952 854
Size: 1850 248 3175
Fragments arranged by size:
3175 1850 248
NcoI C'CATG_G
Cuts at: 107 1515 107
Size: 1408 3865
NdeI CA'TA_TG
Cuts at: 1164 1164
Size: 5273
NgoMIV G'CCGG_C
Cuts at: 3452 3452
Size: 5273
NspI r_CATG'y
Cuts at: 1320 1704 2326 2826 3733 1320
Size: 384 622 500 907 2860
Fragments arranged by size:
2860 907 622 500 384
PasI CC'CwG_GG
Cuts at: 1902 1902
Size: 5273
PciI A'CATG_T
Cuts at: 1700 3729 1700
Size: 2029 3244
PflMI CCAn_nnn'nTGG
Cuts at: 368 368
Size: 5273
PfoI T'CCnGG_A
Cuts at: 1460 1460
Size: 5273
PmeI GTTT'AAAC
Cuts at: 2272 2272
Size: 5273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 4412
Size: 32 5241
PpuMI rG'GwC_Cy
Cuts at: 1719 1719
Size: 5273
PstI C_TGCA'G
Cuts at: 1493 2320 3005 1493
Size: 827 685 3761
Fragments arranged by size:
3761 827 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5273
PvuII CAG'CTG
Cuts at: 2698 3058 2698
Size: 360 4913
RsrII CG'GwC_CG
Cuts at: 3468 3468
Size: 5273
SacI G_AGCT'C
Cuts at: 151 2287 151
Size: 2136 3137
SalI G'TCGA_C
Cuts at: 2310 2310
Size: 5273
SapI GCTCTTCn'nnn_
Cuts at: 3296 3506 3296
Size: 210 5063
SbfI CC_TGCA'GG
Cuts at: 2320 2320
Size: 5273
ScaI AGT'ACT
Cuts at: 1057 4790 1057
Size: 3733 1540
SfcI C'TryA_G
Cuts at: 33 1489 2316 3001 3994 4185 33
Size: 1456 827 685 993 191 1121
Fragments arranged by size:
1456 1121 993 827 685 191
SfoI GGC'GCC
Cuts at: 855 2705 2953 855
Size: 1850 248 3175
Fragments arranged by size:
3175 1850 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
SmaI CCC'GGG
Cuts at: 2295 2295
Size: 5273
SmlI C'TyrA_G
Cuts at: 2428 3343 3835 4097 2428
Size: 915 492 262 3604
Fragments arranged by size:
3604 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5273
SphI G_CATG'C
Cuts at: 2326 2826 2326
Size: 500 4773
StuI AGG'CCT
Cuts at: 220 266 220
Size: 46 5227
StyI C'CwwG_G
Cuts at: 107 114 317 431 615 1515 2118 2407 2761
Size: 7 203 114 184 900 603 289 354
Cuts at: 2761 107
Size: 2619
Fragments arranged by size:
2619 900 603 354 289 203 184 114
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1894 2856 2989 3448 3657 1894
Size: 962 133 459 209 3510
Fragments arranged by size:
3510 962 459 209 133
TatI w'GTAC_w
Cuts at: 236 1055 1856 4788 236
Size: 819 801 2932 721
Fragments arranged by size:
2932 819 801 721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 457 489 1794 1826 4790 4822 457
Size: 32 1305 32 2964 32 908
Fragments arranged by size:
2964 1305 908 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3070 3070
Size: 5273
XbaI T'CTAG_A
Cuts at: 2304 2304
Size: 5273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 1030 2768 438
Size: 592 1738 2943
Fragments arranged by size:
2943 1738 592
XmaI C'CCGG_G
Cuts at: 2293 2293
Size: 5273
XmnI GAAnn'nnTTC
Cuts at: 2113 2113
Size: 5273
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI