Restriction map for
pF1KE2638
(Circular) (Six-base) MAPSORT of: pF1KE2638.seq Check: 2736 from: 1 to: 3905
pF1KE2638 3905 bp
With 182 enzymes: SgfI *
September 8, 2016 13:38 ..
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1730 2162 2909 3177 826
Size: 904 432 747 268 1554
Fragments arranged by size:
1554 904 747 432 268
AfeI AGC'GCT
Cuts at: 176 176
Size: 3905
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 898 1210 2777 898
Size: 312 1567 2026
Fragments arranged by size:
2026 1567 312
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 742 909 3258 3514 742
Size: 167 2349 256 1133
Fragments arranged by size:
2349 1133 256 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 524 925 524
Size: 401 3504
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 921 1335 1583 1618 921
Size: 414 248 35 3208
Fragments arranged by size:
3208 414 248 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BbvCI CC'TCA_GC
Cuts at: 190 190
Size: 3905
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 1017 1017
Size: 3905
Bme1580I G_kGCm'C
Cuts at: 1530 1623 2679 1530
Size: 93 1056 2756
Fragments arranged by size:
2756 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1198 1533 3677 1117
Size: 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 242 220
Size: 22 3883
Bpu10I CC'TnA_GC
Cuts at: 190 190
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 663 796 1081 1960 2452 2750 485
Size: 178 133 285 879 492 298 1640
Fragments arranged by size:
1640 879 492 298 285 178 133
BsaI GGTCTCn'nnnn_
Cuts at: 3609 3609
Size: 3905
BsaAI yAC'GTr
Cuts at: 846 1258 846
Size: 412 3493
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 1336 1584 2286 3782 1336
Size: 248 702 1496 1459
Fragments arranged by size:
1496 1459 702 248
BsaWI w'CCGG_w
Cuts at: 1094 1615 2293 2567 2714 3125 1094
Size: 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 505 535 505
Size: 30 3875
BseYI C'CCAG_C
Cuts at: 439 1330 2088 2665 3161 439
Size: 891 758 577 496 1183
Fragments arranged by size:
1183 891 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 359 521 919 1697 1887 2679 359
Size: 162 398 778 190 792 1585
Fragments arranged by size:
1585 792 778 398 190 162
Bsp1286I G_dGCh'C
Cuts at: 359 521 919 1530 1623 1697 1887 1949 2679
Size: 162 398 611 93 74 190 62 730
Cuts at: 2679 359
Size: 1585
Fragments arranged by size:
1585 730 611 398 190 162 93 74
62
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1169 1471 1852 955
Size: 214 302 381 3008
Fragments arranged by size:
3008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 1903 2084 2293 1903
Size: 181 209 3515
Fragments arranged by size:
3515 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstYI r'GATC_y
Cuts at: 1 930 1397 1420 1755 2001 3013 3099 3111
Size: 929 467 23 335 246 1012 86 12
Cuts at: 3111 3457 1
Size: 346 449
Fragments arranged by size:
1012 929 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3905
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
DraI TTT'AAA
Cuts at: 125 411 904 125
Size: 286 493 3126
Fragments arranged by size:
3126 493 286
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 695 1490 1664 2055 2082 3427 695
Size: 795 174 391 27 1345 1173
Fragments arranged by size:
1345 1173 795 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 464 1928 2138 464
Size: 1464 210 2231
Fragments arranged by size:
2231 1464 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 589 2113 2423 2569 3715 589
Size: 1524 310 146 1146 779
Fragments arranged by size:
1524 1146 779 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 242 826 1730 2162 2909 3177 220
Size: 22 584 904 432 747 268 948
Fragments arranged by size:
948 904 747 584 432 268 22
EcoO109I rG'GnC_Cy
Cuts at: 1044 1044
Size: 3905
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 178 1339 1587 2609 3588 178
Size: 1161 248 1022 979 495
Fragments arranged by size:
1161 1022 979 495 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1747 1777 1779 1809 1747
Size: 30 2 30 3843
Fragments arranged by size:
3843 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 503 2576 2760 503
Size: 2073 184 1648
Fragments arranged by size:
2073 1648 184
MscI TGG'CCA
Cuts at: 697 1666 697
Size: 969 2936
MslI CAynn'nnrTG
Cuts at: 1894 2021 1894
Size: 127 3778
MspA1I CmG'CkG
Cuts at: 439 1016 1330 1690 2703 2948 439
Size: 577 314 360 1013 245 1396
Fragments arranged by size:
1396 1013 577 360 314 245
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NcoI C'CATG_G
Cuts at: 107 107
Size: 3905
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PstI C_TGCA'G
Cuts at: 952 1637 952
Size: 685 3220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 439 1330 1690 439
Size: 891 360 2654
Fragments arranged by size:
2654 891 360
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 948 1633 2626 2817 33
Size: 915 685 993 191 1121
Fragments arranged by size:
1121 993 915 685 191
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 526 927 526
Size: 401 3504
SmlI C'TyrA_G
Cuts at: 500 642 775 1060 1975 2467 2729 500
Size: 142 133 285 915 492 262 1676
Fragments arranged by size:
1676 915 492 285 262 142 133
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
SspI AAT'ATT
Cuts at: 657 657
Size: 3905
StuI AGG'CCT
Cuts at: 506 506
Size: 3905
StyI C'CwwG_G
Cuts at: 107 507 1039 1393 107
Size: 400 532 354 2619
Fragments arranged by size:
2619 532 400 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 3420 3420
Size: 3905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 546 1400 546
Size: 854 3051
XmaI C'CCGG_G
Cuts at: 524 925 524
Size: 401 3504
XmnI GAAnn'nnTTC
Cuts at: 595 656 595
Size: 61 3844
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI