Restriction map for
pF1KE2636
(Circular) (Six-base) MAPSORT of: pF1KE2636.seq Check: 6873 from: 1 to: 4175
pF1KE2636 4175 bp
With 182 enzymes: SgfI *
August 9, 2016 17:37 ..
AatII G_ACGT'C
Cuts at: 503 503
Size: 4175
AccI GT'mk_AC
Cuts at: 1062 1213 1062
Size: 151 4024
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 2432 3179 3447 2000
Size: 432 747 268 2728
Fragments arranged by size:
2728 747 432 268
AflIII A'CryG_T
Cuts at: 1527 2631 3608 1527
Size: 1104 977 2094
Fragments arranged by size:
2094 1104 977
AgeI A'CCGG_T
Cuts at: 198 2563 198
Size: 2365 1810
AhdI GACnn_n'nnGTC
Cuts at: 534 534
Size: 4175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 680 680
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 1480 3047 1480
Size: 1567 2608
ApaI G_GGCC'C
Cuts at: 1047 1047
Size: 4175
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 286 597 886 1179 3528 3784 286
Size: 311 289 293 2349 256 677
Fragments arranged by size:
2349 677 311 293 289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 427 1195 427
Size: 768 3407
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 1191 1605 1853 1888 1191
Size: 414 248 35 3478
Fragments arranged by size:
3478 414 248 35
BanII G_rGCy'C
Cuts at: 211 744 1047 1189 2219 211
Size: 533 303 142 1030 2167
Fragments arranged by size:
2167 1030 533 303 142
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 524 524
Size: 4175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 886 920 886
Size: 34 4141
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 714 1695 714
Size: 981 3194
BglI GCCn_nnn'nGGC
Cuts at: 937 937
Size: 4175
BglII A'GATC_T
Cuts at: 1690 3727 1690
Size: 2037 2138
BlpI GC'TnA_GC
Cuts at: 1287 1287
Size: 4175
Bme1580I G_kGCm'C
Cuts at: 419 1047 1800 1893 2949 419
Size: 628 753 93 1056 1645
Fragments arranged by size:
1645 1056 753 628 93
BmrI ACTGGGnnnn_n'
Cuts at: 263 875 1041 1387 1468 1803 3947 263
Size: 612 166 346 81 335 2144 491
Fragments arranged by size:
2144 612 491 346 335 166 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 940 635
Size: 305 3870
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2722 3020 1351
Size: 879 492 298 2506
Fragments arranged by size:
2506 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3879 3879
Size: 4175
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 500 1606 1854 2556 4052 500
Size: 1106 248 702 1496 623
Fragments arranged by size:
1496 1106 702 623 248
BsaWI w'CCGG_w
Cuts at: 198 1364 1885 2563 2837 2984 3395 198
Size: 1166 521 678 274 147 411 978
Fragments arranged by size:
1166 978 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 913 913
Size: 4175
BseYI C'CCAG_C
Cuts at: 1600 2358 2935 3431 1600
Size: 758 577 496 2344
Fragments arranged by size:
2344 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 2949 1189
Size: 778 190 792 2415
Fragments arranged by size:
2415 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 211 419 744 1047 1189 1800 1893 1967 2157
Size: 208 325 303 142 611 93 74 190
Cuts at: 2157 2219 2949 211
Size: 62 730 1437
Fragments arranged by size:
1437 730 611 325 303 208 190 142
93 74 62
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 575 1225 1439 1741 2122 575
Size: 650 214 302 381 2628
Fragments arranged by size:
2628 650 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2087 2087
Size: 4175
BsrFI r'CCGG_y
Cuts at: 198 2173 2354 2563 198
Size: 1975 181 209 1810
Fragments arranged by size:
1975 1810 209 181
BssSI C'ACGA_G
Cuts at: 2446 2804 2446
Size: 358 3817
BstAPI GCAn_nnn'nTGC
Cuts at: 478 1552 478
Size: 1074 3101
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstYI r'GATC_y
Cuts at: 1 1200 1667 1690 2025 2271 3283 3369 3381
Size: 1199 467 23 335 246 1012 86 12
Cuts at: 3381 3727 1
Size: 346 449
Fragments arranged by size:
1199 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 455 2125 2306 2469 455
Size: 1670 181 163 2161
Fragments arranged by size:
2161 1670 181 163
BtsI GCAGTG_nn'
Cuts at: 697 1418 697
Size: 721 3454
DraI TTT'AAA
Cuts at: 1146 1174 1146
Size: 28 4147
DraIII CAC_nnn'GTG
Cuts at: 1029 1029
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 1037 1760 1934 2325 2352 3697 1037
Size: 723 174 391 27 1345 1515
Fragments arranged by size:
1515 1345 723 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 940 2000 2432 3179 3447 635
Size: 305 1060 432 747 268 1363
Fragments arranged by size:
1363 1060 747 432 305 268
EcoO109I rG'GnC_Cy
Cuts at: 1043 1314 1043
Size: 271 3904
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
EcoRV GAT'ATC
Cuts at: 730 730
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 147 2194 147
Size: 2047 2128
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 1609 1857 2879 3858 1609
Size: 248 1022 979 1926
Fragments arranged by size:
1926 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 719 751 2017 2047 2049 2079 520
Size: 32 167 32 1266 30 2 30 2616
Fragments arranged by size:
2616 1266 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 986 2846 3030 986
Size: 1860 184 2131
Fragments arranged by size:
2131 1860 184
MscI TGG'CCA
Cuts at: 1039 1936 1039
Size: 897 3278
MslI CAynn'nnrTG
Cuts at: 851 2164 2291 851
Size: 1313 127 2735
Fragments arranged by size:
2735 1313 127
MspA1I CmG'CkG
Cuts at: 494 1286 1600 1960 2973 3218 494
Size: 792 314 360 1013 245 1451
Fragments arranged by size:
1451 1013 792 360 314 245
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NspI r_CATG'y
Cuts at: 1228 1728 2635 1228
Size: 500 907 2768
Fragments arranged by size:
2768 907 500
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PshAI GACnn'nnGTC
Cuts at: 352 352
Size: 4175
PspOMI G'GGCC_C
Cuts at: 1043 1043
Size: 4175
PstI C_TGCA'G
Cuts at: 1222 1907 1222
Size: 685 3490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 1600 1960 1600
Size: 360 3815
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 567 1218 1903 2896 3087 33
Size: 534 651 685 993 191 1121
Fragments arranged by size:
1121 993 685 651 534 191
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SgrAI Cr'CCGG_yG
Cuts at: 198 198
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 1330 2245 2737 2999 1330
Size: 915 492 262 2506
Fragments arranged by size:
2506 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StyI C'CwwG_G
Cuts at: 1309 1663 1309
Size: 354 3821
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 282 1758 1891 2350 2559 282
Size: 1476 133 459 209 1898
Fragments arranged by size:
1898 1476 459 209 133
TatI w'GTAC_w
Cuts at: 3690 3690
Size: 4175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4143
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1054 1670 1054
Size: 616 3559
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
ZraI GAC'GTC
Cuts at: 501 501
Size: 4175
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AloI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI XmnI