Restriction map for
pF1KE2633
(Circular) (Six-base) MAPSORT of: pF1KE2633.seq Check: 9946 from: 1 to: 5699
pF1KE2633 5699 bp
With 182 enzymes: SgfI *
August 3, 2016 15:29 ..
AccI GT'mk_AC
Cuts at: 1327 1698 2737 1327
Size: 371 1039 4289
Fragments arranged by size:
4289 1039 371
Acc65I G'GTAC_C
Cuts at: 2715 2715
Size: 5699
AclI AA'CG_TT
Cuts at: 5539 5539
Size: 5699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 298 442 614 743 1010 1075 1163 2233
Size: 165 144 172 129 267 65 88 1070
Cuts at: 2233 2407 2575 3524 3956 4703 4971 133
Size: 174 168 949 432 747 268 861
Fragments arranged by size:
1070 949 861 747 432 268 267 174
172 168 165 144 129 88 65
AflIII A'CryG_T
Cuts at: 1295 3051 4155 5132 1295
Size: 1756 1104 977 1862
Fragments arranged by size:
1862 1756 1104 977
AgeI A'CCGG_T
Cuts at: 4087 4087
Size: 5699
AlwNI CAG_nnn'CTG
Cuts at: 391 721 969 1453 1848 3004 4571 391
Size: 330 248 484 395 1156 1567 1519
Fragments arranged by size:
1567 1519 1156 484 395 330 248
ApaI G_GGCC'C
Cuts at: 124 124
Size: 5699
ApaLI G'TGCA_C
Cuts at: 197 4469 197
Size: 4272 1427
ApoI r'AATT_y
Cuts at: 187 220 241 612 1742 2703 5052 5308 187
Size: 33 21 371 1130 961 2349 256 578
Fragments arranged by size:
2349 1130 961 578 371 256 33 21
AseI AT'TA_AT
Cuts at: 20 179 2174 20
Size: 159 1995 3545
Fragments arranged by size:
3545 1995 159
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
AvaI C'yCGr_G
Cuts at: 1347 2719 1347
Size: 1372 4327
BamHI G'GATC_C
Cuts at: 2609 2724 2609
Size: 115 5584
BanI G'GyrC_C
Cuts at: 2715 3129 3377 3412 2715
Size: 414 248 35 5002
Fragments arranged by size:
5002 414 248 35
BanII G_rGCy'C
Cuts at: 124 1354 1917 2713 3743 124
Size: 1230 563 796 1030 2080
Fragments arranged by size:
2080 1230 1030 796 563
BbeI G_GCGC'C
Cuts at: 3133 3381 3133
Size: 248 5451
BbsI GAAGACnn'nnnn_
Cuts at: 731 1831 1859 2111 2413 731
Size: 1100 28 252 302 4017
Fragments arranged by size:
4017 1100 302 252 28
BciVI GTATCCnnnnn_n'
Cuts at: 3597 4358 5699 3597
Size: 761 1341 3597
Fragments arranged by size:
3597 1341 761
BclI T'GATC_A
Cuts at: 3219 3219
Size: 5699
BglII A'GATC_T
Cuts at: 1892 1928 3214 5251 1892
Size: 36 1286 2037 2340
Fragments arranged by size:
2340 2037 1286 36
BlpI GC'TnA_GC
Cuts at: 2811 2811
Size: 5699
Bme1580I G_kGCm'C
Cuts at: 124 201 540 3324 3417 4473 124
Size: 77 339 2784 93 1056 1350
Fragments arranged by size:
2784 1350 1056 339 93 77
BmrI ACTGGGnnnn_n'
Cuts at: 1179 2911 2992 3327 5471 1179
Size: 1732 81 335 2144 1407
Fragments arranged by size:
2144 1732 1407 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4066 4066
Size: 5699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 763 1286 1471 1563 2047 535
Size: 228 523 185 92 484 4187
Fragments arranged by size:
4187 523 484 228 185 92
Bpu10I CC'TnA_GC
Cuts at: 1798 2474 1798
Size: 676 5023
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1151 2434 2875 3754 4246 4544 335
Size: 816 1283 441 879 492 298 1490
Fragments arranged by size:
1490 1283 879 816 492 441 298
BsaI GGTCTCn'nnnn_
Cuts at: 1763 5403 1763
Size: 3640 2059
BsaAI yAC'GTr
Cuts at: 196 944 2199 3052 196
Size: 748 1255 853 2843
Fragments arranged by size:
2843 1255 853 748
BsaBI GATnn'nnATC
Cuts at: 6 3213 5250 6
Size: 3207 2037 455
Fragments arranged by size:
3207 2037 455
BsaHI Gr'CG_yC
Cuts at: 3130 3378 4080 5576 3130
Size: 248 702 1496 3253
Fragments arranged by size:
3253 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2888 3409 4087 4361 4508 4919 2888
Size: 521 678 274 147 411 3668
Fragments arranged by size:
3668 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 619 1216 1564 283
Size: 336 597 348 4418
Fragments arranged by size:
4418 597 348 336
BseYI C'CCAG_C
Cuts at: 131 335 3124 3882 4459 4955 131
Size: 204 2789 758 577 496 875
Fragments arranged by size:
2789 875 758 577 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 1883 2617 290
Size: 1593 734 3372
Fragments arranged by size:
3372 1593 734
BsiEI CG_ry'CG
Cuts at: 104 3241 3287 4495 104
Size: 3137 46 1208 1308
Fragments arranged by size:
3137 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 201 497 1323 2713 3491 3681 4473 201
Size: 296 826 1390 778 190 792 1427
Fragments arranged by size:
1427 1390 826 792 778 296 190
Bsp1286I G_dGCh'C
Cuts at: 124 201 497 540 1323 1354 1917 2713 3324
Size: 77 296 43 783 31 563 796 611
Cuts at: 3324 3417 3491 3681 3743 4473 124
Size: 93 74 190 62 730 1350
Fragments arranged by size:
1350 796 783 730 611 563 296 190
93 77 74 62 43 31
BspEI T'CCGG_A
Cuts at: 4919 4919
Size: 5699
BspHI T'CATG_A
Cuts at: 4875 4875
Size: 5699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2749 2963 3265 3646 2749
Size: 214 302 381 4802
Fragments arranged by size:
4802 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2607 3991 4045 5529 5694 50
Size: 2557 1384 54 1484 165 55
Fragments arranged by size:
2557 1484 1384 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1354 3611 1354
Size: 2257 3442
BsrFI r'CCGG_y
Cuts at: 3697 3878 4087 3697
Size: 181 209 5309
Fragments arranged by size:
5309 209 181
BssSI C'ACGA_G
Cuts at: 3970 4328 3970
Size: 358 5341
BstAPI GCAn_nnn'nTGC
Cuts at: 1396 2079 3076 1396
Size: 683 997 4019
Fragments arranged by size:
4019 997 683
BstBI TT'CG_AA
Cuts at: 330 4060 330
Size: 3730 1969
BstYI r'GATC_y
Cuts at: 1 273 1269 1892 1928 2243 2609 2724 3191
Size: 272 996 623 36 315 366 115 467
Cuts at: 3191 3214 3549 3795 4807 4893 4905 5251 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 996 623 467 449 366 346 335
315 272 246 115 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 508 508
Size: 5699
BtgI C'CryG_G
Cuts at: 107 1221 107
Size: 1114 4585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1555 3649 3830 3993 1555
Size: 2094 181 163 3261
Fragments arranged by size:
3261 2094 181 163
BtsI GCAGTG_nn'
Cuts at: 2134 2942 2134
Size: 808 4891
DraI TTT'AAA
Cuts at: 1302 2264 2698 1302
Size: 962 434 4303
Fragments arranged by size:
4303 962 434
DrdI GACnn_nn'nnGTC
Cuts at: 3405 4263 3405
Size: 858 4841
EaeI y'GGCC_r
Cuts at: 3284 3458 3849 3876 5221 3284
Size: 174 391 27 1345 3762
Fragments arranged by size:
3762 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3284 3284
Size: 5699
EarI CTCTTCn'nnn_
Cuts at: 152 274 1193 1553 1934 2021 2441 2498 3722
Size: 122 919 360 381 87 420 57 1224
Cuts at: 3722 3932 152
Size: 210 1919
Fragments arranged by size:
1919 1224 919 420 381 360 210 122
87 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3907 4217 4363 5509 3907
Size: 310 146 1146 4097
Fragments arranged by size:
4097 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2711 2711
Size: 5699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 298 442 535 614 743 763 1010 1075
Size: 165 144 93 79 129 20 247 65
Cuts at: 1075 1163 1286 1471 1563 2047 2233 2407 2575
Size: 88 123 185 92 484 186 174 168
Cuts at: 2575 3524 3956 4703 4971 133
Size: 949 432 747 268 861
Fragments arranged by size:
949 861 747 484 432 268 247 186
185 174 168 165 144 129 123 93
92 88 79 65 20
EcoO109I rG'GnC_Cy
Cuts at: 2838 2838
Size: 5699
EcoRI G'AATT_C
Cuts at: 1742 2703 1742
Size: 961 4738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 3718 2290
Size: 1428 4271
FspI TGC'GCA
Cuts at: 3480 3480
Size: 5699
HaeII r_GCGC'y
Cuts at: 3133 3381 4403 5382 3133
Size: 248 1022 979 3450
Fragments arranged by size:
3450 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1092 1124 1435 1456 1467 1488 1552 1584 3541
Size: 32 311 21 11 21 64 32 1957
Cuts at: 3541 3571 3573 3603 1092
Size: 30 2 30 3188
Fragments arranged by size:
3188 1957 311 64 32 32 30 30
21 21 11 2
HincII GTy'rAC
Cuts at: 1511 2738 1511
Size: 1227 4472
HindIII A'AGCT_T
Cuts at: 1367 2575 1367
Size: 1208 4491
HpaI GTT'AAC
Cuts at: 1511 1511
Size: 5699
KasI G'GCGC_C
Cuts at: 3129 3377 3129
Size: 248 5451
KpnI G_GTAC'C
Cuts at: 2719 2719
Size: 5699
MluI A'CGCG_T
Cuts at: 5132 5132
Size: 5699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1062 2360 4370 4554 1062
Size: 1298 2010 184 2207
Fragments arranged by size:
2207 2010 1298 184
MscI TGG'CCA
Cuts at: 3460 3460
Size: 5699
MslI CAynn'nnrTG
Cuts at: 2176 3688 3815 2176
Size: 1512 127 4060
Fragments arranged by size:
4060 1512 127
MspA1I CmG'CkG
Cuts at: 339 1791 2810 3124 3484 4497 4742 339
Size: 1452 1019 314 360 1013 245 1296
Fragments arranged by size:
1452 1296 1019 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3880 3880
Size: 5699
NarI GG'CG_CC
Cuts at: 3130 3378 3130
Size: 248 5451
NcoI C'CATG_G
Cuts at: 107 107
Size: 5699
NgoMIV G'CCGG_C
Cuts at: 3878 3878
Size: 5699
NspI r_CATG'y
Cuts at: 1002 1299 2086 2752 3252 4159 1002
Size: 297 787 666 500 907 2542
Fragments arranged by size:
2542 907 787 666 500 297
PasI CC'CwG_GG
Cuts at: 1590 1590
Size: 5699
PciI A'CATG_T
Cuts at: 1295 4155 1295
Size: 2860 2839
PflMI CCAn_nnn'nTGG
Cuts at: 903 969 1596 903
Size: 66 627 5006
Fragments arranged by size:
5006 627 66
PmeI GTTT'AAAC
Cuts at: 2698 2698
Size: 5699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 484 516 4838 4870 484
Size: 32 4322 32 1313
Fragments arranged by size:
4322 1313 32 32
PspOMI G'GGCC_C
Cuts at: 120 120
Size: 5699
PstI C_TGCA'G
Cuts at: 2746 3431 2746
Size: 685 5014
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5699
PvuII CAG'CTG
Cuts at: 339 1791 3124 3484 339
Size: 1452 1333 360 2554
Fragments arranged by size:
2554 1452 1333 360
RsrII CG'GwC_CG
Cuts at: 662 3894 662
Size: 3232 2467
SacI G_AGCT'C
Cuts at: 2713 2713
Size: 5699
SalI G'TCGA_C
Cuts at: 2736 2736
Size: 5699
SapI GCTCTTCn'nnn_
Cuts at: 2021 2441 3722 3932 2021
Size: 420 1281 210 3788
Fragments arranged by size:
3788 1281 420 210
SbfI CC_TGCA'GG
Cuts at: 2746 2746
Size: 5699
ScaI AGT'ACT
Cuts at: 5216 5216
Size: 5699
SexAI A'CCwGG_T
Cuts at: 1772 1772
Size: 5699
SfcI C'TryA_G
Cuts at: 33 413 850 1328 1786 2126 2742 3427 4420
Size: 380 437 478 458 340 616 685 993
Cuts at: 4420 4611 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 616 478 458 437 380
340 191
SfoI GGC'GCC
Cuts at: 3131 3379 3131
Size: 248 5451
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
SmaI CCC'GGG
Cuts at: 2721 2721
Size: 5699
SmlI C'TyrA_G
Cuts at: 350 1166 2413 2854 3769 4261 4523 350
Size: 816 1247 441 915 492 262 1526
Fragments arranged by size:
1526 1247 915 816 492 441 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5699
SphI G_CATG'C
Cuts at: 2752 3252 2752
Size: 500 5199
SspI AAT'ATT
Cuts at: 369 369
Size: 5699
StuI AGG'CCT
Cuts at: 748 1921 748
Size: 1173 4526
StyI C'CwwG_G
Cuts at: 107 1476 2627 2833 3187 107
Size: 1369 1151 206 354 2619
Fragments arranged by size:
2619 1369 1151 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 650 3282 3415 3874 4083 650
Size: 2632 133 459 209 2266
Fragments arranged by size:
2632 2266 459 209 133
TatI w'GTAC_w
Cuts at: 5214 5214
Size: 5699
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5216 5248 5216
Size: 32 5667
Tth111I GACn'n_nGTC
Cuts at: 1566 3496 1566
Size: 1930 3769
XbaI T'CTAG_A
Cuts at: 807 2730 807
Size: 1923 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1495 3194 1495
Size: 1699 4000
XmaI C'CCGG_G
Cuts at: 2719 2719
Size: 5699
XmnI GAAnn'nnTTC
Cuts at: 479 479
Size: 5699
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI