Restriction map for
pF1KE2631
(Circular) (Six-base) MAPSORT of: pF1KE2631.seq Check: 5222 from: 1 to: 6461
pF1KE2631 6461 bp
With 182 enzymes: SgfI *
July 8, 2016 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 832 832
Size: 6461
AccI GT'mk_AC
Cuts at: 1442 3499 1442
Size: 2057 4404
Acc65I G'GTAC_C
Cuts at: 3477 3477
Size: 6461
AclI AA'CG_TT
Cuts at: 2537 6301 2537
Size: 3764 2697
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1269 2221 3189 4286 4718 5465 5733 1269
Size: 952 968 1097 432 747 268 1997
Fragments arranged by size:
1997 1097 968 952 747 432 268
AfeI AGC'GCT
Cuts at: 2566 2566
Size: 6461
AflIII A'CryG_T
Cuts at: 1814 3813 4917 5894 1814
Size: 1999 1104 977 2381
Fragments arranged by size:
2381 1999 1104 977
AgeI A'CCGG_T
Cuts at: 4849 4849
Size: 6461
AleI CACnn'nnGTG
Cuts at: 472 814 838 3265 472
Size: 342 24 2427 3668
Fragments arranged by size:
3668 2427 342 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 832 2638 832
Size: 1806 4655
AlwNI CAG_nnn'CTG
Cuts at: 1848 2130 3766 5333 1848
Size: 282 1636 1567 2976
Fragments arranged by size:
2976 1636 1567 282
ApaI G_GGCC'C
Cuts at: 628 1626 1719 2099 3169 628
Size: 998 93 380 1070 3920
Fragments arranged by size:
3920 1070 998 380 93
ApaLI G'TGCA_C
Cuts at: 2167 2602 5231 2167
Size: 435 2629 3397
Fragments arranged by size:
3397 2629 435
ApoI r'AATT_y
Cuts at: 3465 5814 6070 3465
Size: 2349 256 3856
Fragments arranged by size:
3856 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6461
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6461
AvaI C'yCGr_G
Cuts at: 156 1647 2395 3299 3379 3481 156
Size: 1491 748 904 80 102 3136
Fragments arranged by size:
3136 1491 904 748 102 80
BamHI G'GATC_C
Cuts at: 1576 3486 1576
Size: 1910 4551
BanI G'GyrC_C
Cuts at: 400 640 700 808 1408 2245 2297 2444 2645
Size: 240 60 108 600 837 52 147 201
Cuts at: 2645 3477 3891 4139 4174 400
Size: 832 414 248 35 2687
Fragments arranged by size:
2687 837 832 600 414 248 240 201
147 108 60 52 35
BanII G_rGCy'C
Cuts at: 628 1189 1626 1719 2099 2233 3169 3475 4505
Size: 561 437 93 380 134 936 306 1030
Cuts at: 4505 628
Size: 2584
Fragments arranged by size:
2584 1030 936 561 437 380 306 134
93
BbeI G_GCGC'C
Cuts at: 2301 2448 3895 4143 2301
Size: 147 1447 248 4619
Fragments arranged by size:
4619 1447 248 147
BbsI GAAGACnn'nnnn_
Cuts at: 873 1275 2827 873
Size: 402 1552 4507
Fragments arranged by size:
4507 1552 402
BbvCI CC'TCA_GC
Cuts at: 1852 1852
Size: 6461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1242 1276 3022 3056 90
Size: 34 1118 34 1746 34 3495
Fragments arranged by size:
3495 1746 1118 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2033 4359 5120 6461 2033
Size: 2326 761 1341 2033
Fragments arranged by size:
2326 2033 1341 761
BclI T'GATC_A
Cuts at: 3981 3981
Size: 6461
BfrBI ATG'CAT
Cuts at: 2787 2865 2787
Size: 78 6383
BglI GCCn_nnn'nGGC
Cuts at: 1021 2278 1021
Size: 1257 5204
BglII A'GATC_T
Cuts at: 3976 6013 3976
Size: 2037 4424
BlpI GC'TnA_GC
Cuts at: 3573 3573
Size: 6461
Bme1580I G_kGCm'C
Cuts at: 403 628 670 703 1540 1626 1667 1719 2099
Size: 225 42 33 837 86 41 52 380
Cuts at: 2099 2171 2248 2606 3169 4086 4179 5235 403
Size: 72 77 358 563 917 93 1056 1629
Fragments arranged by size:
1629 1056 917 837 563 380 358 225
93 86 77 72 52 42 41 33
BmgBI CAC'GTC
Cuts at: 2468 2468
Size: 6461
BmrI ACTGGGnnnn_n'
Cuts at: 3164 3673 3754 4089 6233 3164
Size: 509 81 335 2144 3392
Fragments arranged by size:
3392 2144 509 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2586 2635 4828 2586
Size: 49 2193 4219
Fragments arranged by size:
4219 2193 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1203 1887 2107 2136 1203
Size: 684 220 29 5528
Fragments arranged by size:
5528 684 220 29
Bpu10I CC'TnA_GC
Cuts at: 1852 2561 1852
Size: 709 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 3637 4516 5008 5306 2260
Size: 1377 879 492 298 3415
Fragments arranged by size:
3415 1377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 623 6165 623
Size: 5542 919
BsaAI yAC'GTr
Cuts at: 3814 3814
Size: 6461
BsaBI GATnn'nnATC
Cuts at: 6 1750 2677 3975 6012 6
Size: 1744 927 1298 2037 455
Fragments arranged by size:
2037 1744 1298 927 455
BsaHI Gr'CG_yC
Cuts at: 446 2298 2445 3305 3892 4140 4842 6338 446
Size: 1852 147 860 587 248 702 1496 569
Fragments arranged by size:
1852 1496 860 702 587 569 248 147
BsaWI w'CCGG_w
Cuts at: 2930 3108 3650 4171 4849 5123 5270 5681 2930
Size: 178 542 521 678 274 147 411 3710
Fragments arranged by size:
3710 678 542 521 411 274 178 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1435 1465 1435
Size: 30 6431
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 271 1065 1233 2337 3025 3028 3094 3171
Size: 3 794 168 1104 688 3 66 77
Cuts at: 3171 268
Size: 3558
Fragments arranged by size:
3558 1104 794 688 168 77 66 3
3
BseYI C'CCAG_C
Cuts at: 732 922 1600 2174 2195 2273 3046 3055 3886
Size: 190 678 574 21 78 773 9 831
Cuts at: 3886 4644 5221 5717 732
Size: 758 577 496 1476
Fragments arranged by size:
1476 831 773 758 678 577 574 496
190 78 21 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1856 1861 3040 403
Size: 1453 5 1179 3824
Fragments arranged by size:
3824 1453 1179 5
BsiEI CG_ry'CG
Cuts at: 104 3266 3432 4003 4049 5257 104
Size: 3162 166 571 46 1208 1308
Fragments arranged by size:
3162 1308 1208 571 166 46
BsiHKAI G_wGCw'C
Cuts at: 821 1189 2171 2606 3045 3398 3475 4253 4443
Size: 368 982 435 439 353 77 778 190
Cuts at: 4443 5235 821
Size: 792 2047
Fragments arranged by size:
2047 982 792 778 439 435 368 353
190 77
BsmBI CGTCTCn'nnnn_
Cuts at: 1033 2974 1033
Size: 1941 4520
Bsp1286I G_dGCh'C
Cuts at: 403 628 670 703 821 1189 1540 1626 1667
Size: 225 42 33 118 368 351 86 41
Cuts at: 1667 1719 2099 2171 2233 2248 2606 3045 3169
Size: 52 380 72 62 15 358 439 124
Cuts at: 3169 3398 3475 4086 4179 4253 4443 4505 5235
Size: 229 77 611 93 74 190 62 730
Cuts at: 5235 403
Size: 1629
Fragments arranged by size:
1629 730 611 439 380 368 358 351
229 225 190 124 118 93 86 77
74 72 62 62 52 42 41 33
15
BspEI T'CCGG_A
Cuts at: 2930 3108 5681 2930
Size: 178 2573 3710
Fragments arranged by size:
3710 2573 178
BspHI T'CATG_A
Cuts at: 5637 5637
Size: 6461
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 428 832 1665 3511 3725 4027 4408 326
Size: 102 404 833 1846 214 302 381 2379
Fragments arranged by size:
2379 1846 833 404 381 302 214 102
BsrBI CCG'CTC
Cuts at: 50 1367 2883 2889 4753 4807 6291 6456 50
Size: 1317 1516 6 1864 54 1484 165 55
Fragments arranged by size:
1864 1516 1484 1317 165 55 54 6
BsrDI GCAATG_nn'
Cuts at: 482 1757 1976 3357 4373 482
Size: 1275 219 1381 1016 2570
Fragments arranged by size:
2570 1381 1275 1016 219
BsrFI r'CCGG_y
Cuts at: 378 4459 4640 4849 378
Size: 4081 181 209 1990
Fragments arranged by size:
4081 1990 209 181
BsrGI T'GTAC_A
Cuts at: 689 689
Size: 6461
BssSI C'ACGA_G
Cuts at: 1528 2918 4732 5090 1528
Size: 1390 1814 358 2899
Fragments arranged by size:
2899 1814 1390 358
BstAPI GCAn_nnn'nTGC
Cuts at: 760 1848 3838 760
Size: 1088 1990 3383
Fragments arranged by size:
3383 1990 1088
BstBI TT'CG_AA
Cuts at: 4822 4822
Size: 6461
BstEII G'GTnAC_C
Cuts at: 976 1044 2526 976
Size: 68 1482 4911
Fragments arranged by size:
4911 1482 68
BstXI CCAn_nnnn'nTGG
Cuts at: 276 621 1559 276
Size: 345 938 5178
Fragments arranged by size:
5178 938 345
BstYI r'GATC_y
Cuts at: 1 1133 1576 2727 2849 2894 3105 3486 3953
Size: 1132 443 1151 122 45 211 381 467
Cuts at: 3953 3976 4311 4557 5569 5655 5667 6013 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1151 1132 1012 467 449 443 381 346
335 246 211 122 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 1378 3086 1378
Size: 1708 4753
BtgI C'CryG_G
Cuts at: 644 737 996 1558 1686 2010 3420 644
Size: 93 259 562 128 324 1410 3685
Fragments arranged by size:
3685 1410 562 324 259 128 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 155 1407 2048 4411 4592 4755 155
Size: 1252 641 2363 181 163 1861
Fragments arranged by size:
2363 1861 1252 641 181 163
BtsI GCAGTG_nn'
Cuts at: 1844 2759 3704 1844
Size: 915 945 4601
Fragments arranged by size:
4601 945 915
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 345 380 345
Size: 35 6426
DraI TTT'AAA
Cuts at: 3460 3460
Size: 6461
DrdI GACnn_nn'nnGTC
Cuts at: 4167 5025 4167
Size: 858 5603
EaeI y'GGCC_r
Cuts at: 302 545 740 830 911 1244 1418 1549 1683
Size: 243 195 90 81 333 174 131 134
Cuts at: 1683 2508 3263 3429 4046 4220 4611 4638 5983
Size: 825 755 166 617 174 391 27 1345
Cuts at: 5983 302
Size: 780
Fragments arranged by size:
1345 825 780 755 617 391 333 243
195 174 174 166 134 131 90 81
27
EagI C'GGCC_G
Cuts at: 3263 3429 4046 3263
Size: 166 617 5678
Fragments arranged by size:
5678 617 166
EarI CTCTTCn'nnn_
Cuts at: 338 460 733 2279 2972 2993 4484 4694 338
Size: 122 273 1546 693 21 1491 210 2105
Fragments arranged by size:
2105 1546 1491 693 273 210 122 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2676 2950 4669 4979 5125 6271 2676
Size: 274 1719 310 146 1146 2866
Fragments arranged by size:
2866 1719 1146 310 274 146
EcoICRI GAG'CTC
Cuts at: 1187 3473 1187
Size: 2286 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1203 1269 1887 2107 2136 2221 3189 4286 4718
Size: 66 618 220 29 85 968 1097 432
Cuts at: 4718 5465 5733 1203
Size: 747 268 1931
Fragments arranged by size:
1931 1097 968 747 618 432 268 220
85 66 29
EcoNI CCTnn'n_nnAGG
Cuts at: 322 1198 1636 1771 2305 322
Size: 876 438 135 534 4478
Fragments arranged by size:
4478 876 534 438 135
EcoO109I rG'GnC_Cy
Cuts at: 387 1062 1374 1469 1622 1623 1716 2095 2096
Size: 675 312 95 153 1 93 379 1
Cuts at: 2096 2214 2391 3165 3166 3384 3600 387
Size: 118 177 774 1 218 216 3248
Fragments arranged by size:
3248 774 675 379 312 218 216 177
153 118 95 93 1 1 1
EcoRI G'AATT_C
Cuts at: 3465 3465
Size: 6461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 957 3219 4480 957
Size: 2262 1261 2938
Fragments arranged by size:
2938 2262 1261
FspI TGC'GCA
Cuts at: 4242 4242
Size: 6461
HaeII r_GCGC'y
Cuts at: 2301 2448 2568 3895 4143 5165 6144 2301
Size: 147 120 1327 248 1022 979 2618
Fragments arranged by size:
2618 1327 1022 979 248 147 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1282 1314 2031 2063 2353 2385 2912 2925 2944
Size: 32 717 32 290 32 527 13 19
Cuts at: 2944 2957 3018 3050 4303 4333 4335 4365 1282
Size: 13 61 32 1253 30 2 30 3378
Fragments arranged by size:
3378 1253 717 527 290 61 32 32
32 32 30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 864 3500 864
Size: 2636 3825
HindIII A'AGCT_T
Cuts at: 3310 3310
Size: 6461
KasI G'GCGC_C
Cuts at: 2297 2444 3891 4139 2297
Size: 147 1447 248 4619
Fragments arranged by size:
4619 1447 248 147
KpnI G_GTAC'C
Cuts at: 3481 3481
Size: 6461
MfeI C'AATT_G
Cuts at: 563 563
Size: 6461
MluI A'CGCG_T
Cuts at: 5894 5894
Size: 6461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2375 3266 5132 5316 2375
Size: 891 1866 184 3520
Fragments arranged by size:
3520 1866 891 184
MscI TGG'CCA
Cuts at: 304 742 832 913 1420 1551 1685 2510 4222
Size: 438 90 81 507 131 134 825 1712
Cuts at: 4222 304
Size: 2543
Fragments arranged by size:
2543 1712 825 507 438 134 131 90
81
MslI CAynn'nnrTG
Cuts at: 406 412 472 553 814 826 838 1056 1277
Size: 6 60 81 261 12 12 218 221
Cuts at: 1277 1557 1819 2620 2716 3265 4450 4577 406
Size: 280 262 801 96 549 1185 127 2290
Fragments arranged by size:
2290 1185 801 549 280 262 261 221
218 127 96 81 60 12 12 6
MspA1I CmG'CkG
Cuts at: 2127 2178 2199 2277 2506 3024 3050 3422 3572
Size: 51 21 78 229 518 26 372 150
Cuts at: 3572 3886 4246 5259 5504 2127
Size: 314 360 1013 245 3084
Fragments arranged by size:
3084 1013 518 372 360 314 245 229
150 78 51 26 21
NaeI GCC'GGC
Cuts at: 4642 4642
Size: 6461
NarI GG'CG_CC
Cuts at: 2298 2445 3892 4140 2298
Size: 147 1447 248 4619
Fragments arranged by size:
4619 1447 248 147
NcoI C'CATG_G
Cuts at: 737 996 1558 1686 737
Size: 259 562 128 5512
Fragments arranged by size:
5512 562 259 128
NgoMIV G'CCGG_C
Cuts at: 4640 4640
Size: 6461
NotI GC'GGCC_GC
Cuts at: 3429 3429
Size: 6461
NsiI A_TGCA'T
Cuts at: 2789 2867 2789
Size: 78 6383
NspI r_CATG'y
Cuts at: 798 1818 2619 3258 3330 3514 4014 4921 798
Size: 1020 801 639 72 184 500 907 2338
Fragments arranged by size:
2338 1020 907 801 639 500 184 72
PasI CC'CwG_GG
Cuts at: 324 3073 324
Size: 2749 3712
PciI A'CATG_T
Cuts at: 1814 4917 1814
Size: 3103 3358
PflMI CCAn_nnn'nTGG
Cuts at: 634 2114 2163 634
Size: 1480 49 4932
Fragments arranged by size:
4932 1480 49
PfoI T'CCnGG_A
Cuts at: 2492 2492
Size: 6461
PmeI GTTT'AAAC
Cuts at: 3460 3460
Size: 6461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5600 5632 5600
Size: 32 6429
PpuMI rG'GwC_Cy
Cuts at: 1374 3384 1374
Size: 2010 4451
PspOMI G'GGCC_C
Cuts at: 624 1622 1715 2095 3165 624
Size: 998 93 380 1070 3920
Fragments arranged by size:
3920 1070 998 380 93
PstI C_TGCA'G
Cuts at: 434 1610 1640 1676 2408 3508 4193 434
Size: 1176 30 36 732 1100 685 2702
Fragments arranged by size:
2702 1176 1100 732 685 36 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6461
PvuII CAG'CTG
Cuts at: 2127 2178 2199 2506 3024 3050 3886 4246 2127
Size: 51 21 307 518 26 836 360 4342
Fragments arranged by size:
4342 836 518 360 307 51 26 21
RsrII CG'GwC_CG
Cuts at: 4656 4656
Size: 6461
SacI G_AGCT'C
Cuts at: 1189 3475 1189
Size: 2286 4175
SacII CC_GC'GG
Cuts at: 3423 3423
Size: 6461
SalI G'TCGA_C
Cuts at: 3498 3498
Size: 6461
SapI GCTCTTCn'nnn_
Cuts at: 4484 4694 4484
Size: 210 6251
SbfI CC_TGCA'GG
Cuts at: 1676 3508 1676
Size: 1832 4629
ScaI AGT'ACT
Cuts at: 5978 5978
Size: 6461
SfcI C'TryA_G
Cuts at: 33 430 1606 1636 1672 2404 3504 4189 5182
Size: 397 1176 30 36 732 1100 685 993
Cuts at: 5182 5373 33
Size: 191 1121
Fragments arranged by size:
1176 1121 1100 993 732 685 397 191
36 30
SfoI GGC'GCC
Cuts at: 2299 2446 3893 4141 2299
Size: 147 1447 248 4619
Fragments arranged by size:
4619 1447 248 147
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6461
SmaI CCC'GGG
Cuts at: 158 3301 3483 158
Size: 3143 182 3136
Fragments arranged by size:
3143 3136 182
SmlI C'TyrA_G
Cuts at: 1647 2239 3379 3616 4531 5023 5285 1647
Size: 592 1140 237 915 492 262 2823
Fragments arranged by size:
2823 1140 915 592 492 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6461
SphI G_CATG'C
Cuts at: 2619 3258 3514 4014 2619
Size: 639 256 500 5066
Fragments arranged by size:
5066 639 500 256
StyI C'CwwG_G
Cuts at: 173 737 996 1199 1558 1686 3134 3595 3949
Size: 564 259 203 359 128 1448 461 354
Cuts at: 3949 173
Size: 2685
Fragments arranged by size:
2685 1448 564 461 359 354 259 203
128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 462 838 966 1875 4044 4177 4636 4845 462
Size: 376 128 909 2169 133 459 209 2078
Fragments arranged by size:
2169 2078 909 459 376 209 133 128
TatI w'GTAC_w
Cuts at: 689 1997 5976 689
Size: 1308 3979 1174
Fragments arranged by size:
3979 1308 1174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 941 973 5978 6010 941
Size: 32 5005 32 1392
Fragments arranged by size:
5005 1392 32 32
Tth111I GACn'n_nGTC
Cuts at: 4258 4258
Size: 6461
XbaI T'CTAG_A
Cuts at: 3492 3492
Size: 6461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 1144 3956 693
Size: 451 2812 3198
Fragments arranged by size:
3198 2812 451
XhoI C'TCGA_G
Cuts at: 1647 3379 1647
Size: 1732 4729
XmaI C'CCGG_G
Cuts at: 156 3299 3481 156
Size: 3143 182 3136
Fragments arranged by size:
3143 3136 182
XmnI GAAnn'nnTTC
Cuts at: 2326 2326
Size: 6461
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BmtI
BsiWI BsmI BssHII BstZ17I ClaI DraIII EcoRV FseI
FspAI HpaI NdeI NheI NruI PacI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI