Restriction map for
pF1KE2611
(Circular) (Six-base) MAPSORT of: pF1KE2611.seq Check: 7765 from: 1 to: 3998
pF1KE2611 3998 bp
With 182 enzymes: SgfI *
February 29, 2016 12:19 ..
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 801 1823 2255 3002 3270 801
Size: 1022 432 747 268 1529
Fragments arranged by size:
1529 1022 747 432 268
AflIII A'CryG_T
Cuts at: 1350 2454 3431 1350
Size: 1104 977 1917
Fragments arranged by size:
1917 1104 977
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 153 743 925 1303 2870 153
Size: 590 182 378 1567 1281
Fragments arranged by size:
1567 1281 590 378 182
ApaI G_GGCC'C
Cuts at: 859 859
Size: 3998
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 1002 3351 3607 1002
Size: 2349 256 1393
Fragments arranged by size:
2349 1393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 761 1018 761
Size: 257 3741
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 1014 1428 1676 1711 1014
Size: 414 248 35 3301
Fragments arranged by size:
3301 414 248 35
BanII G_rGCy'C
Cuts at: 432 669 686 859 1012 2042 432
Size: 237 17 173 153 1030 2388
Fragments arranged by size:
2388 1030 237 173 153 17
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 807 807
Size: 3998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3964
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 1518 1518
Size: 3998
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 171 1110 171
Size: 939 3059
Bme1580I G_kGCm'C
Cuts at: 205 859 1623 1716 2772 205
Size: 654 764 93 1056 1431
Fragments arranged by size:
1431 1056 764 654 93
BmrI ACTGGGnnnn_n'
Cuts at: 426 1210 1291 1626 3770 426
Size: 784 81 335 2144 654
Fragments arranged by size:
2144 784 654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 945 2365 945
Size: 1420 2578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 636
Size: 3998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2053 2545 2843 1174
Size: 879 492 298 2329
Fragments arranged by size:
2329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 758 3702 758
Size: 2944 1054
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 434 464 434
Size: 30 3968
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 613
Size: 3998
BseYI C'CCAG_C
Cuts at: 360 561 756 800 1423 2181 2758 3254 360
Size: 201 195 44 623 758 577 496 1104
Fragments arranged by size:
1104 758 623 577 496 201 195 44
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1012 1790 1980 2772 1012
Size: 778 190 792 2238
Fragments arranged by size:
2238 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 205 432 669 686 859 1012 1623 1716 1790
Size: 227 237 17 173 153 611 93 74
Cuts at: 1790 1980 2042 2772 205
Size: 190 62 730 1431
Fragments arranged by size:
1431 730 611 237 227 190 173 153
93 74 62 17
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1262 1564 1945 1048
Size: 214 302 381 3101
Fragments arranged by size:
3101 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 320 383 686 713 1910 320
Size: 63 303 27 1197 2408
Fragments arranged by size:
2408 1197 303 63 27
BsrFI r'CCGG_y
Cuts at: 1996 2177 2386 1996
Size: 181 209 3608
Fragments arranged by size:
3608 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstXI CCAn_nnnn'nTGG
Cuts at: 327 660 327
Size: 333 3665
BstYI r'GATC_y
Cuts at: 1 1023 1490 1513 1848 2094 3106 3192 3204
Size: 1022 467 23 335 246 1012 86 12
Cuts at: 3204 3550 1
Size: 346 449
Fragments arranged by size:
1022 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 116 116
Size: 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 785 1241 785
Size: 456 3542
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 317 608 1583 1757 2148 2175 3520 317
Size: 291 975 174 391 27 1345 795
Fragments arranged by size:
1345 975 795 391 291 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 157 2021 2231 157
Size: 1864 210 1924
Fragments arranged by size:
1924 1864 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 801 1823 2255 3002 3270 636
Size: 165 1022 432 747 268 1364
Fragments arranged by size:
1364 1022 747 432 268 165
EcoO109I rG'GnC_Cy
Cuts at: 856 1137 856
Size: 281 3717
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 186 2017 186
Size: 1831 2167
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 1432 1680 2702 3681 1432
Size: 248 1022 979 1749
Fragments arranged by size:
1749 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 263 295 872 904 1840 1870 1872 1902 263
Size: 32 577 32 936 30 2 30 2359
Fragments arranged by size:
2359 936 577 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
HindIII A'AGCT_T
Cuts at: 138 138
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 273 2669 2853 273
Size: 2396 184 1418
Fragments arranged by size:
2396 1418 184
MscI TGG'CCA
Cuts at: 319 610 1759 319
Size: 291 1149 2558
Fragments arranged by size:
2558 1149 291
MslI CAynn'nnrTG
Cuts at: 325 1987 2114 325
Size: 1662 127 2209
Fragments arranged by size:
2209 1662 127
MspA1I CmG'CkG
Cuts at: 364 1109 1423 1783 2796 3041 364
Size: 745 314 360 1013 245 1321
Fragments arranged by size:
1321 1013 745 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 1051 1551 2458 1051
Size: 500 907 2591
Fragments arranged by size:
2591 907 500
PasI CC'CwG_GG
Cuts at: 726 726
Size: 3998
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PflMI CCAn_nnn'nTGG
Cuts at: 438 438
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PshAI GACnn'nnGTC
Cuts at: 492 770 492
Size: 278 3720
PspOMI G'GGCC_C
Cuts at: 855 855
Size: 3998
PstI C_TGCA'G
Cuts at: 1045 1730 1045
Size: 685 3313
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 364 1423 1783 364
Size: 1059 360 2579
Fragments arranged by size:
2579 1059 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 157 2021 2231 157
Size: 1864 210 1924
Fragments arranged by size:
1924 1864 210
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 848 1041 1726 2719 2910 33
Size: 815 193 685 993 191 1121
Fragments arranged by size:
1121 993 815 685 193 191
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 761 1153 2068 2560 2822 761
Size: 392 915 492 262 1937
Fragments arranged by size:
1937 915 492 392 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StuI AGG'CCT
Cuts at: 677 884 677
Size: 207 3791
StyI C'CwwG_G
Cuts at: 222 791 1132 1486 222
Size: 569 341 354 2734
Fragments arranged by size:
2734 569 354 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1581 1714 2173 2382 1581
Size: 133 459 209 3197
Fragments arranged by size:
3197 459 209 133
TatI w'GTAC_w
Cuts at: 3513 3513
Size: 3998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 434 466 3515 3547 434
Size: 32 3049 32 885
Fragments arranged by size:
3049 885 32 32
Tth111I GACn'n_nGTC
Cuts at: 931 1795 931
Size: 864 3134
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XhoI C'TCGA_G
Cuts at: 761 761
Size: 3998
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
XmnI GAAnn'nnTTC
Cuts at: 403 403
Size: 3998
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI