Restriction map for
pF1KE2604
(Circular) (Six-base) MAPSORT of: pF1KE2604.seq Check: 1939 from: 1 to: 3650
pF1KE2604 3650 bp
With 182 enzymes: SgfI *
January 19, 2016 12:01 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 666 666
Size: 3650
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 1907 2654 2922 1475
Size: 432 747 268 2203
Fragments arranged by size:
2203 747 432 268
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 2038 2038
Size: 3650
AleI CACnn'nnGTG
Cuts at: 521 521
Size: 3650
AlwNI CAG_nnn'CTG
Cuts at: 387 955 2522 387
Size: 568 1567 1515
Fragments arranged by size:
1567 1515 568
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 394 654 3003 3259 394
Size: 260 2349 256 785
Fragments arranged by size:
2349 785 260 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 670 670
Size: 3650
BamHI G'GATC_C
Cuts at: 675 675
Size: 3650
BanI G'GyrC_C
Cuts at: 666 1080 1328 1363 666
Size: 414 248 35 2953
Fragments arranged by size:
2953 414 248 35
BanII G_rGCy'C
Cuts at: 664 1694 664
Size: 1030 2620
BbeI G_GCGC'C
Cuts at: 1084 1332 1084
Size: 248 3402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3616
BciVI GTATCCnnnnn_n'
Cuts at: 193 1548 2309 3650 193
Size: 1355 761 1341 193
Fragments arranged by size:
1355 1341 761 193
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglI GCCn_nnn'nGGC
Cuts at: 160 160
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 762 762
Size: 3650
Bme1580I G_kGCm'C
Cuts at: 1275 1368 2424 1275
Size: 93 1056 2501
Fragments arranged by size:
2501 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 862 943 1278 3422 862
Size: 81 335 2144 1090
Fragments arranged by size:
2144 1090 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 527 2017 527
Size: 1490 2160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 539 439
Size: 100 3550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 415 3354 415
Size: 2939 711
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 1164 3201 6
Size: 1158 2037 455
Fragments arranged by size:
2037 1158 455
BsaHI Gr'CG_yC
Cuts at: 1081 1329 2031 3527 1081
Size: 248 702 1496 1204
Fragments arranged by size:
1496 1204 702 248
BsaWI w'CCGG_w
Cuts at: 839 1360 2038 2312 2459 2870 839
Size: 521 678 274 147 411 1619
Fragments arranged by size:
1619 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 597 597
Size: 3650
BseYI C'CCAG_C
Cuts at: 1075 1833 2410 2906 1075
Size: 758 577 496 1819
Fragments arranged by size:
1819 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 160 1192 1238 2446 104
Size: 56 1032 46 1208 1308
Fragments arranged by size:
1308 1208 1032 56 46
BsiHKAI G_wGCw'C
Cuts at: 664 1442 1632 2424 664
Size: 778 190 792 1890
Fragments arranged by size:
1890 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 664 1275 1368 1442 1632 1694 2424 664
Size: 611 93 74 190 62 730 1890
Fragments arranged by size:
1890 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 914 1216 1597 700
Size: 214 302 381 2753
Fragments arranged by size:
2753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1942 1996 3480 3645 50
Size: 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1562
Size: 3650
BsrFI r'CCGG_y
Cuts at: 159 1648 1829 2038 159
Size: 1489 181 209 1771
Fragments arranged by size:
1771 1489 209 181
BssSI C'ACGA_G
Cuts at: 521 1921 2279 521
Size: 1400 358 1892
Fragments arranged by size:
1892 1400 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 2011 2011
Size: 3650
BstXI CCAn_nnnn'nTGG
Cuts at: 304 304
Size: 3650
BstYI r'GATC_y
Cuts at: 1 675 1142 1165 1500 1746 2758 2844 2856
Size: 674 467 23 335 246 1012 86 12
Cuts at: 2856 3202 1
Size: 346 449
Fragments arranged by size:
1012 674 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 297 303 297
Size: 6 3644
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 893 893
Size: 3650
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 300 1235 1409 1800 1827 3172 300
Size: 935 174 391 27 1345 778
Fragments arranged by size:
1345 935 778 391 174 27
EagI C'GGCC_G
Cuts at: 1235 1235
Size: 3650
EarI CTCTTCn'nnn_
Cuts at: 322 1673 1883 322
Size: 1351 210 2089
Fragments arranged by size:
2089 1351 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 2168 2314 3460 1858
Size: 310 146 1146 2048
Fragments arranged by size:
2048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 662 662
Size: 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 539 1475 1907 2654 2922 439
Size: 100 936 432 747 268 1167
Fragments arranged by size:
1167 936 747 432 268 100
EcoNI CCTnn'n_nnAGG
Cuts at: 501 501
Size: 3650
EcoO109I rG'GnC_Cy
Cuts at: 789 789
Size: 3650
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 1669 228
Size: 1441 2209
FspI TGC'GCA
Cuts at: 1431 1431
Size: 3650
HaeII r_GCGC'y
Cuts at: 1084 1332 2354 3333 1084
Size: 248 1022 979 1401
Fragments arranged by size:
1401 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 361 393 1492 1522 1524 1554 361
Size: 32 1099 30 2 30 2457
Fragments arranged by size:
2457 1099 32 30 30 2
HincII GTy'rAC
Cuts at: 689 689
Size: 3650
KasI G'GCGC_C
Cuts at: 1080 1328 1080
Size: 248 3402
KpnI G_GTAC'C
Cuts at: 670 670
Size: 3650
MfeI C'AATT_G
Cuts at: 365 365
Size: 3650
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 177 2321 2505 177
Size: 2144 184 1322
Fragments arranged by size:
2144 1322 184
MscI TGG'CCA
Cuts at: 302 1411 302
Size: 1109 2541
MslI CAynn'nnrTG
Cuts at: 302 521 1639 1766 302
Size: 219 1118 127 2186
Fragments arranged by size:
2186 1118 219 127
MspA1I CmG'CkG
Cuts at: 550 761 1075 1435 2448 2693 550
Size: 211 314 360 1013 245 1507
Fragments arranged by size:
1507 1013 360 314 245 211
NaeI GCC'GGC
Cuts at: 1831 1831
Size: 3650
NarI GG'CG_CC
Cuts at: 1081 1329 1081
Size: 248 3402
NcoI C'CATG_G
Cuts at: 297 303 297
Size: 6 3644
NgoMIV G'CCGG_C
Cuts at: 1829 1829
Size: 3650
NspI r_CATG'y
Cuts at: 703 1203 2110 703
Size: 500 907 2243
Fragments arranged by size:
2243 907 500
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PfoI T'CCnGG_A
Cuts at: 263 263
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PshAI GACnn'nnGTC
Cuts at: 427 427
Size: 3650
PstI C_TGCA'G
Cuts at: 534 697 1382 534
Size: 163 685 2802
Fragments arranged by size:
2802 685 163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 1075 1435 1075
Size: 360 3290
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 664 664
Size: 3650
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SfcI C'TryA_G
Cuts at: 33 530 693 1378 2371 2562 33
Size: 497 163 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 191 163
SfoI GGC'GCC
Cuts at: 1082 1330 1082
Size: 248 3402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 672 672
Size: 3650
SmlI C'TyrA_G
Cuts at: 805 1720 2212 2474 805
Size: 915 492 262 1981
Fragments arranged by size:
1981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 703 1203 703
Size: 500 3150
StyI C'CwwG_G
Cuts at: 297 303 784 1138 297
Size: 6 481 354 2809
Fragments arranged by size:
2809 481 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 386 588 632 1233 1366 1825 2034 386
Size: 202 44 601 133 459 209 2002
Fragments arranged by size:
2002 601 459 209 202 133 44
TatI w'GTAC_w
Cuts at: 216 3165 216
Size: 2949 701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 245 277 3167 3199 245
Size: 32 2890 32 696
Fragments arranged by size:
2890 696 32 32
Tth111I GACn'n_nGTC
Cuts at: 1447 1447
Size: 3650
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 1145
Size: 3650
XmaI C'CCGG_G
Cuts at: 670 670
Size: 3650
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI