Restriction map for
pF1KE2601
(Circular) (Six-base) MAPSORT of: pF1KE2601.seq Check: 3166 from: 1 to: 5297
pF1KE2601 5297 bp
With 182 enzymes: SgfI *
January 19, 2016 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 763 1960 763
Size: 1197 4100
AccI GT'mk_AC
Cuts at: 2335 2335
Size: 5297
Acc65I G'GTAC_C
Cuts at: 2313 2313
Size: 5297
AclI AA'CG_TT
Cuts at: 5137 5137
Size: 5297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1516 3122 3554 4301 4569 229
Size: 1287 1606 432 747 268 957
Fragments arranged by size:
1606 1287 957 747 432 268
AflIII A'CryG_T
Cuts at: 2649 3753 4730 2649
Size: 1104 977 3216
Fragments arranged by size:
3216 1104 977
AgeI A'CCGG_T
Cuts at: 3685 3685
Size: 5297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2107 2107
Size: 5297
AlwNI CAG_nnn'CTG
Cuts at: 180 2602 4169 180
Size: 2422 1567 1308
Fragments arranged by size:
2422 1567 1308
ApaI G_GGCC'C
Cuts at: 516 516
Size: 5297
ApaLI G'TGCA_C
Cuts at: 2236 4067 2236
Size: 1831 3466
ApoI r'AATT_y
Cuts at: 302 559 892 1137 1612 1743 2050 2165 2301
Size: 257 333 245 475 131 307 115 136
Cuts at: 2301 4650 4906 302
Size: 2349 256 693
Fragments arranged by size:
2349 693 475 333 307 257 256 245
136 131 115
AseI AT'TA_AT
Cuts at: 20 20
Size: 5297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
AvaI C'yCGr_G
Cuts at: 1961 2317 1961
Size: 356 4941
BamHI G'GATC_C
Cuts at: 2322 2322
Size: 5297
BanI G'GyrC_C
Cuts at: 2313 2727 2975 3010 2313
Size: 414 248 35 4600
Fragments arranged by size:
4600 414 248 35
BanII G_rGCy'C
Cuts at: 516 1790 2311 3341 516
Size: 1274 521 1030 2472
Fragments arranged by size:
2472 1274 1030 521
BbeI G_GCGC'C
Cuts at: 2731 2979 2731
Size: 248 5049
BbvCI CC'TCA_GC
Cuts at: 768 1313 768
Size: 545 4752
BciVI GTATCCnnnnn_n'
Cuts at: 1640 3195 3956 5297 1640
Size: 1555 761 1341 1640
Fragments arranged by size:
1640 1555 1341 761
BclI T'GATC_A
Cuts at: 758 2817 758
Size: 2059 3238
BfrBI ATG'CAT
Cuts at: 1196 1196
Size: 5297
BglII A'GATC_T
Cuts at: 2812 4849 2812
Size: 2037 3260
BlpI GC'TnA_GC
Cuts at: 187 2409 187
Size: 2222 3075
Bme1580I G_kGCm'C
Cuts at: 516 2240 2922 3015 4071 516
Size: 1724 682 93 1056 1742
Fragments arranged by size:
1742 1724 1056 682 93
BmrI ACTGGGnnnn_n'
Cuts at: 1791 2509 2590 2925 5069 1791
Size: 718 81 335 2144 2019
Fragments arranged by size:
2144 2019 718 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1376 3664 1376
Size: 2288 3009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2159 2270 1153
Size: 1006 111 4180
Fragments arranged by size:
4180 1006 111
Bpu10I CC'TnA_GC
Cuts at: 768 1238 1313 768
Size: 470 75 4752
Fragments arranged by size:
4752 470 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 2473 3352 3844 4142 830
Size: 1643 879 492 298 1985
Fragments arranged by size:
1985 1643 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 811 5001 811
Size: 4190 1107
BsaAI yAC'GTr
Cuts at: 1892 2650 1892
Size: 758 4539
BsaBI GATnn'nnATC
Cuts at: 6 840 1628 2811 4848 6
Size: 834 788 1183 2037 455
Fragments arranged by size:
2037 1183 834 788 455
BsaHI Gr'CG_yC
Cuts at: 2728 2976 3678 5174 2728
Size: 248 702 1496 2851
Fragments arranged by size:
2851 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2486 3007 3685 3959 4106 4517 2486
Size: 521 678 274 147 411 3266
Fragments arranged by size:
3266 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2102 2132 2162 2243 2273 2102
Size: 30 30 81 30 5126
Fragments arranged by size:
5126 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 335 335
Size: 5297
BseYI C'CCAG_C
Cuts at: 447 1102 1286 2209 2722 3480 4057 4553 447
Size: 655 184 923 513 758 577 496 1191
Fragments arranged by size:
1191 923 758 655 577 513 496 184
BsiEI CG_ry'CG
Cuts at: 104 2839 2885 4093 104
Size: 2735 46 1208 1308
Fragments arranged by size:
2735 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 979 1728 1790 2240 2311 3089 3279 4071 979
Size: 749 62 450 71 778 190 792 2205
Fragments arranged by size:
2205 792 778 749 450 190 71 62
BsmI GAATG_Cn'
Cuts at: 954 1198 1711 1757 1985 954
Size: 244 513 46 228 4266
Fragments arranged by size:
4266 513 244 228 46
BsmBI CGTCTCn'nnnn_
Cuts at: 2130 2135 2130
Size: 5 5292
Bsp1286I G_dGCh'C
Cuts at: 516 979 1728 1790 2240 2311 2922 3015 3089
Size: 463 749 62 450 71 611 93 74
Cuts at: 3089 3279 3341 4071 516
Size: 190 62 730 1742
Fragments arranged by size:
1742 749 730 611 463 450 190 93
74 71 62 62
BspEI T'CCGG_A
Cuts at: 4517 4517
Size: 5297
BspHI T'CATG_A
Cuts at: 755 4473 755
Size: 3718 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 763 1960 2347 2561 2863 3244 763
Size: 1197 387 214 302 381 2816
Fragments arranged by size:
2816 1197 387 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3589 3643 5127 5292 50
Size: 3539 54 1484 165 55
Fragments arranged by size:
3539 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 797 3209 797
Size: 2412 2885
BsrFI r'CCGG_y
Cuts at: 3295 3476 3685 3295
Size: 181 209 4907
Fragments arranged by size:
4907 209 181
BsrGI T'GTAC_A
Cuts at: 522 2006 522
Size: 1484 3813
BssSI C'ACGA_G
Cuts at: 2278 3568 3926 2278
Size: 1290 358 3649
Fragments arranged by size:
3649 1290 358
BstAPI GCAn_nnn'nTGC
Cuts at: 183 2674 183
Size: 2491 2806
BstBI TT'CG_AA
Cuts at: 3658 3658
Size: 5297
BstXI CCAn_nnnn'nTGG
Cuts at: 573 573
Size: 5297
BstYI r'GATC_y
Cuts at: 1 2271 2322 2789 2812 3147 3393 4405 4491
Size: 2270 51 467 23 335 246 1012 86
Cuts at: 4491 4503 4849 1
Size: 12 346 449
Fragments arranged by size:
2270 1012 467 449 346 335 246 86
51 23 12
BtgI C'CryG_G
Cuts at: 107 626 1109 107
Size: 519 483 4295
Fragments arranged by size:
4295 519 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 884 1066 3247 3428 3591 884
Size: 182 2181 181 163 2590
Fragments arranged by size:
2590 2181 182 181 163
BtsI GCAGTG_nn'
Cuts at: 2091 2540 2091
Size: 449 4848
DraI TTT'AAA
Cuts at: 2296 2296
Size: 5297
DrdI GACnn_nn'nnGTC
Cuts at: 3003 3861 3003
Size: 858 4439
EaeI y'GGCC_r
Cuts at: 166 2120 2882 3056 3447 3474 4819 166
Size: 1954 762 174 391 27 1345 644
Fragments arranged by size:
1954 1345 762 644 391 174 27
EagI C'GGCC_G
Cuts at: 2882 2882
Size: 5297
EarI CTCTTCn'nnn_
Cuts at: 809 1041 1492 3320 3530 809
Size: 232 451 1828 210 2576
Fragments arranged by size:
2576 1828 451 232 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 439 3505 3815 3961 5107 439
Size: 3066 310 146 1146 629
Fragments arranged by size:
3066 1146 629 310 146
EcoICRI GAG'CTC
Cuts at: 1788 2309 1788
Size: 521 4776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1153 1516 2159 2270 3122 3554 4301 4569
Size: 924 363 643 111 852 432 747 268
Cuts at: 4569 229
Size: 957
Fragments arranged by size:
957 924 852 747 643 432 363 268
111
EcoNI CCTnn'n_nnAGG
Cuts at: 1216 1216
Size: 5297
EcoO109I rG'GnC_Cy
Cuts at: 512 1317 2436 512
Size: 805 1119 3373
Fragments arranged by size:
3373 1119 805
EcoRI G'AATT_C
Cuts at: 2301 2301
Size: 5297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3316 3316
Size: 5297
FspI TGC'GCA
Cuts at: 796 3078 796
Size: 2282 3015
FspAI rTGC'GCAy
Cuts at: 796 796
Size: 5297
HaeII r_GCGC'y
Cuts at: 199 2731 2979 4001 4980 199
Size: 2532 248 1022 979 516
Fragments arranged by size:
2532 1022 979 516 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 978 1010 3139 3169 3171 3201 978
Size: 32 2129 30 2 30 3074
Fragments arranged by size:
3074 2129 32 30 30 2
HincII GTy'rAC
Cuts at: 2336 2336
Size: 5297
KasI G'GCGC_C
Cuts at: 2727 2975 2727
Size: 248 5049
KpnI G_GTAC'C
Cuts at: 2317 2317
Size: 5297
MluI A'CGCG_T
Cuts at: 4730 4730
Size: 5297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1437 2241 3968 4152 1437
Size: 804 1727 184 2582
Fragments arranged by size:
2582 1727 804 184
MscI TGG'CCA
Cuts at: 3058 3058
Size: 5297
MslI CAynn'nnrTG
Cuts at: 389 947 2188 3286 3413 389
Size: 558 1241 1098 127 2273
Fragments arranged by size:
2273 1241 1098 558 127
MspA1I CmG'CkG
Cuts at: 138 180 2209 2408 2722 3082 4095 4340 138
Size: 42 2029 199 314 360 1013 245 1095
Fragments arranged by size:
2029 1095 1013 360 314 245 199 42
NaeI GCC'GGC
Cuts at: 3478 3478
Size: 5297
NarI GG'CG_CC
Cuts at: 2728 2976 2728
Size: 248 5049
NcoI C'CATG_G
Cuts at: 107 626 107
Size: 519 4778
NdeI CA'TA_TG
Cuts at: 1470 1470
Size: 5297
NgoMIV G'CCGG_C
Cuts at: 3476 3476
Size: 5297
NsiI A_TGCA'T
Cuts at: 1198 1198
Size: 5297
NspI r_CATG'y
Cuts at: 217 335 616 795 1656 2350 2850 3757 217
Size: 118 281 179 861 694 500 907 1757
Fragments arranged by size:
1757 907 861 694 500 281 179 118
PciI A'CATG_T
Cuts at: 3753 3753
Size: 5297
PflMI CCAn_nnn'nTGG
Cuts at: 535 1348 1683 1892 535
Size: 813 335 209 3940
Fragments arranged by size:
3940 813 335 209
PfoI T'CCnGG_A
Cuts at: 1217 1217
Size: 5297
PmeI GTTT'AAAC
Cuts at: 2296 2296
Size: 5297
PmlI CAC'GTG
Cuts at: 1892 1892
Size: 5297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 4436
Size: 32 5265
PpuMI rG'GwC_Cy
Cuts at: 1317 1317
Size: 5297
PspOMI G'GGCC_C
Cuts at: 512 512
Size: 5297
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 246 278 246
Size: 32 5265
PstI C_TGCA'G
Cuts at: 2344 3029 2344
Size: 685 4612
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5297
PvuII CAG'CTG
Cuts at: 180 2209 2722 3082 180
Size: 2029 513 360 2395
Fragments arranged by size:
2395 2029 513 360
RsrII CG'GwC_CG
Cuts at: 3492 3492
Size: 5297
SacI G_AGCT'C
Cuts at: 1790 2311 1790
Size: 521 4776
SalI G'TCGA_C
Cuts at: 2334 2334
Size: 5297
SapI GCTCTTCn'nnn_
Cuts at: 1041 3320 3530 1041
Size: 2279 210 2808
Fragments arranged by size:
2808 2279 210
SbfI CC_TGCA'GG
Cuts at: 2344 2344
Size: 5297
ScaI AGT'ACT
Cuts at: 4814 4814
Size: 5297
SexAI A'CCwGG_T
Cuts at: 1386 1386
Size: 5297
SfcI C'TryA_G
Cuts at: 33 1067 2340 3025 4018 4209 33
Size: 1034 1273 685 993 191 1121
Fragments arranged by size:
1273 1121 1034 993 685 191
SfoI GGC'GCC
Cuts at: 2729 2977 2729
Size: 248 5049
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
SmaI CCC'GGG
Cuts at: 2319 2319
Size: 5297
SmlI C'TyrA_G
Cuts at: 845 1961 2452 3367 3859 4121 845
Size: 1116 491 915 492 262 2021
Fragments arranged by size:
2021 1116 915 492 491 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5297
SphI G_CATG'C
Cuts at: 795 2350 2850 795
Size: 1555 500 3242
Fragments arranged by size:
3242 1555 500
SspI AAT'ATT
Cuts at: 1093 1551 1093
Size: 458 4839
StuI AGG'CCT
Cuts at: 766 766
Size: 5297
StyI C'CwwG_G
Cuts at: 107 626 2431 2785 107
Size: 519 1805 354 2619
Fragments arranged by size:
2619 1805 519 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2880 3013 3472 3681 2880
Size: 133 459 209 4496
Fragments arranged by size:
4496 459 209 133
TatI w'GTAC_w
Cuts at: 221 522 2006 4812 221
Size: 301 1484 2806 706
Fragments arranged by size:
2806 1484 706 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 4814 4846 2102
Size: 32 2680 32 2553
Fragments arranged by size:
2680 2553 32 32
Tth111I GACn'n_nGTC
Cuts at: 1213 3094 1213
Size: 1881 3416
XbaI T'CTAG_A
Cuts at: 2328 2328
Size: 5297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2792 2792
Size: 5297
XhoI C'TCGA_G
Cuts at: 1961 1961
Size: 5297
XmaI C'CCGG_G
Cuts at: 2317 2317
Size: 5297
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BcgI BglI BmgBI BmtI BsgI BsiWI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI HindIII HpaI MfeI NheI NotI NruI PacI
PasI PshAI PsiI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XmnI ZraI