Restriction map for
pF1KE2597
(Circular) (Six-base) MAPSORT of: pF1KE2597.seq Check: 6366 from: 1 to: 8930
pF1KE2597 8930 bp
With 182 enzymes: SgfI *
January 12, 2016 10:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1362 1362
Size: 8930
AccI GT'mk_AC
Cuts at: 1409 5637 5968 1409
Size: 4228 331 4371
Fragments arranged by size:
4371 4228 331
Acc65I G'GTAC_C
Cuts at: 874 5946 874
Size: 5072 3858
AclI AA'CG_TT
Cuts at: 8770 8770
Size: 8930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1127 1271 2055 2327 2333 2819 4723 5140 5446
Size: 144 784 272 6 486 1904 417 306
Cuts at: 5446 5554 5786 6755 7187 7934 8202 1127
Size: 108 232 969 432 747 268 1855
Fragments arranged by size:
1904 1855 969 784 747 486 432 417
306 272 268 232 144 108 6
AfeI AGC'GCT
Cuts at: 433 3526 433
Size: 3093 5837
AflIII A'CryG_T
Cuts at: 1592 4326 6282 7386 8363 1592
Size: 2734 1956 1104 977 2159
Fragments arranged by size:
2734 2159 1956 1104 977
AgeI A'CCGG_T
Cuts at: 7318 7318
Size: 8930
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1717 1749 3613 3645 5219 5251 1717
Size: 32 1864 32 1574 32 5396
Fragments arranged by size:
5396 1864 1574 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 712 1180 1455 2008 3138 3768 3924 4308 5265
Size: 468 275 553 1130 630 156 384 957
Cuts at: 5265 5562 5718 6235 7802 712
Size: 297 156 517 1567 1840
Fragments arranged by size:
1840 1567 1130 957 630 553 517 468
384 297 275 156 156
ApaLI G'TGCA_C
Cuts at: 7700 7700
Size: 8930
ApoI r'AATT_y
Cuts at: 853 1127 1204 2079 2389 2683 4629 5803 5934
Size: 274 77 875 310 294 1946 1174 131
Cuts at: 5934 8283 8539 853
Size: 2349 256 1244
Fragments arranged by size:
2349 1946 1244 1174 875 310 294 274
256 131 77
AseI AT'TA_AT
Cuts at: 20 20
Size: 8930
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8930
AvaI C'yCGr_G
Cuts at: 3503 3539 4023 5950 3503
Size: 36 484 1927 6483
Fragments arranged by size:
6483 1927 484 36
AvrII C'CTAG_G
Cuts at: 1321 1321
Size: 8930
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 858 891 858
Size: 33 8897
BamHI G'GATC_C
Cuts at: 863 5813 5955 863
Size: 4950 142 3838
Fragments arranged by size:
4950 3838 142
BanI G'GyrC_C
Cuts at: 874 1867 2164 5946 6360 6608 6643 874
Size: 993 297 3782 414 248 35 3161
Fragments arranged by size:
3782 3161 993 414 297 248 35
BanII G_rGCy'C
Cuts at: 1685 2435 5237 5944 6974 1685
Size: 750 2802 707 1030 3641
Fragments arranged by size:
3641 2802 1030 750 707
BbeI G_GCGC'C
Cuts at: 6364 6612 6364
Size: 248 8682
BbsI GAAGACnn'nnnn_
Cuts at: 330 667 1966 2416 4372 5351 5693 330
Size: 337 1299 450 1956 979 342 3567
Fragments arranged by size:
3567 1956 1299 979 450 342 337
BbvCI CC'TCA_GC
Cuts at: 2197 2897 3591 4319 5030 5417 5878 2197
Size: 700 694 728 711 387 461 5249
Fragments arranged by size:
5249 728 711 700 694 461 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3793 3827 5202 5236 3793
Size: 34 1375 34 7487
Fragments arranged by size:
7487 1375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5067 6828 7589 8930 5067
Size: 1761 761 1341 5067
Fragments arranged by size:
5067 1761 1341 761
BclI T'GATC_A
Cuts at: 635 1022 3926 5288 6450 635
Size: 387 2904 1362 1162 3115
Fragments arranged by size:
3115 2904 1362 1162 387
BfrBI ATG'CAT
Cuts at: 1188 1188
Size: 8930
BglI GCCn_nnn'nGGC
Cuts at: 3505 3583 3685 5389 5413 3505
Size: 78 102 1704 24 7022
Fragments arranged by size:
7022 1704 102 78 24
BglII A'GATC_T
Cuts at: 1002 1296 1514 6445 8482 1002
Size: 294 218 4931 2037 1450
Fragments arranged by size:
4931 2037 1450 294 218
BlpI GC'TnA_GC
Cuts at: 1235 2921 3089 4301 5519 6042 1235
Size: 1686 168 1212 1218 523 4123
Fragments arranged by size:
4123 1686 1218 1212 523 168
Bme1580I G_kGCm'C
Cuts at: 1079 4104 6555 6648 7704 1079
Size: 3025 2451 93 1056 2305
Fragments arranged by size:
3025 2451 2305 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 722 2376 4221 6142 6223 6558 8702 722
Size: 1654 1845 1921 81 335 2144 950
Fragments arranged by size:
2144 1921 1845 1654 950 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2129 3516 3849 7297 2129
Size: 1387 333 3448 3762
Fragments arranged by size:
3762 3448 1387 333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 817 940 1555 1624 2770 2968 3100 3493
Size: 491 123 615 69 1146 198 132 393
Cuts at: 3493 3568 3673 3790 4297 4366 5329 5476 5584
Size: 75 105 117 507 69 963 147 108
Cuts at: 5584 5695 5848 326
Size: 111 153 3408
Fragments arranged by size:
3408 1146 963 615 507 491 393 198
153 147 132 123 117 111 108 105
75 69 69
Bpu10I CC'TnA_GC
Cuts at: 2197 2897 3591 4319 5030 5417 5878 2197
Size: 700 694 728 711 387 461 5249
Fragments arranged by size:
5249 728 711 700 694 461 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2124 2953 3010 3290 4381 4828 4945 5053 6106
Size: 829 57 280 1091 447 117 108 1053
Cuts at: 6106 6985 7477 7775 2124
Size: 879 492 298 3279
Fragments arranged by size:
3279 1091 1053 879 829 492 447 298
280 117 108 57
BsaI GGTCTCn'nnnn_
Cuts at: 3284 4973 8634 3284
Size: 1689 3661 3580
Fragments arranged by size:
3661 3580 1689
BsaAI yAC'GTr
Cuts at: 6283 6283
Size: 8930
BsaBI GATnn'nnATC
Cuts at: 6 3799 6444 8481 6
Size: 3793 2645 2037 455
Fragments arranged by size:
3793 2645 2037 455
BsaHI Gr'CG_yC
Cuts at: 6361 6609 7311 8807 6361
Size: 248 702 1496 6484
Fragments arranged by size:
6484 1496 702 248
BsaWI w'CCGG_w
Cuts at: 640 3251 5102 6119 6640 7318 7592 7739 8150
Size: 2611 1851 1017 521 678 274 147 411
Cuts at: 8150 640
Size: 1420
Fragments arranged by size:
2611 1851 1420 1017 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 329 559 589 610 640 1528 1558 1717
Size: 30 230 30 21 30 888 30 159
Cuts at: 1717 1747 2142 2172 3613 3643 5219 5249 299
Size: 30 395 30 1441 30 1576 30 3980
Fragments arranged by size:
3980 1576 1441 888 395 230 159 30
30 30 30 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 828 1240 1627 1738 1963 2121 2455 2626 3172
Size: 412 387 111 225 158 334 171 546
Cuts at: 3172 3481 3490 3565 3670 3925 4096 4177 4180
Size: 309 9 75 105 255 171 81 3
Cuts at: 4180 4285 4294 4768 5191 5223 5434 5845 828
Size: 105 9 474 423 32 211 411 3913
Fragments arranged by size:
3913 546 474 423 412 411 387 334
309 255 225 211 171 171 158 111
105 105 81 75 32 9 9 3
BseYI C'CCAG_C
Cuts at: 1078 2465 5078 6355 7113 7690 8186 1078
Size: 1387 2613 1277 758 577 496 1822
Fragments arranged by size:
2613 1822 1387 1277 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1387 2514 2666 5251 283
Size: 1104 1127 152 2585 3962
Fragments arranged by size:
3962 2585 1127 1104 152
BsiEI CG_ry'CG
Cuts at: 104 6472 6518 7726 104
Size: 6368 46 1208 1308
Fragments arranged by size:
6368 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1395 1685 2113 4918 5237 5944 6722 6912 7704
Size: 290 428 2805 319 707 778 190 792
Cuts at: 7704 1395
Size: 2621
Fragments arranged by size:
2805 2621 792 778 707 428 319 290
190
BsmI GAATG_Cn'
Cuts at: 4347 4581 4347
Size: 234 8696
Bsp1286I G_dGCh'C
Cuts at: 1079 1395 1685 2113 2435 4104 4918 5237 5944
Size: 316 290 428 322 1669 814 319 707
Cuts at: 5944 6555 6648 6722 6912 6974 7704 1079
Size: 611 93 74 190 62 730 2305
Fragments arranged by size:
2305 1669 814 730 707 611 428 322
319 316 290 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3251 5102 8150 3251
Size: 1851 3048 4031
Fragments arranged by size:
4031 3048 1851
BspHI T'CATG_A
Cuts at: 2582 4403 8106 2582
Size: 1821 3703 3406
Fragments arranged by size:
3703 3406 1821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1362 3155 3755 4151 5165 5504 5980 6194 6496
Size: 1793 600 396 1014 339 476 214 302
Cuts at: 6496 6877 1362
Size: 381 3415
Fragments arranged by size:
3415 1793 1014 600 476 396 381 339
302 214
BsrBI CCG'CTC
Cuts at: 50 1996 3383 3495 4544 5460 7222 7276 8760
Size: 1946 1387 112 1049 916 1762 54 1484
Cuts at: 8760 8925 50
Size: 165 55
Fragments arranged by size:
1946 1762 1484 1387 1049 916 165 112
55 54
BsrDI GCAATG_nn'
Cuts at: 1344 1499 1883 1961 3083 4205 5373 5622 5852
Size: 155 384 78 1122 1122 1168 249 230
Cuts at: 5852 6842 1344
Size: 990 3432
Fragments arranged by size:
3432 1168 1122 1122 990 384 249 230
155 78
BsrFI r'CCGG_y
Cuts at: 1888 3577 6928 7109 7318 1888
Size: 1689 3351 181 209 3500
Fragments arranged by size:
3500 3351 1689 209 181
BsrGI T'GTAC_A
Cuts at: 416 1376 416
Size: 960 7970
BssHII G'CGCG_C
Cuts at: 3952 3952
Size: 8930
BssSI C'ACGA_G
Cuts at: 400 5821 7201 7559 400
Size: 5421 1380 358 1771
Fragments arranged by size:
5421 1771 1380 358
BstAPI GCAn_nnn'nTGC
Cuts at: 703 1390 6307 703
Size: 687 4917 3326
Fragments arranged by size:
4917 3326 687
BstBI TT'CG_AA
Cuts at: 7291 7291
Size: 8930
BstEII G'GTnAC_C
Cuts at: 1434 1434
Size: 8930
BstXI CCAn_nnnn'nTGG
Cuts at: 5022 5022
Size: 8930
BstYI r'GATC_y
Cuts at: 1 863 1002 1296 1514 3226 3258 4472 4790
Size: 862 139 294 218 1712 32 1214 318
Cuts at: 4790 5552 5813 5955 6422 6445 6780 7026 8038
Size: 762 261 142 467 23 335 246 1012
Cuts at: 8038 8124 8136 8482 1
Size: 86 12 346 449
Fragments arranged by size:
1712 1214 1012 862 762 467 449 346
335 318 294 261 246 218 142 139
86 32 23 12
Bsu36I CC'TnA_GG
Cuts at: 236 1835 3694 5038 236
Size: 1599 1859 1344 4128
Fragments arranged by size:
4128 1859 1599 1344
BtgI C'CryG_G
Cuts at: 1871 2168 3680 1871
Size: 297 1512 7121
Fragments arranged by size:
7121 1512 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6880 7061 7224 6880
Size: 181 163 8586
Fragments arranged by size:
8586 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 6173 1391
Size: 4782 4148
ClaI AT'CG_AT
Cuts at: 2327 3803 2327
Size: 1476 7454
DraI TTT'AAA
Cuts at: 5929 5929
Size: 8930
DraIII CAC_nnn'GTG
Cuts at: 2127 2127
Size: 8930
DrdI GACnn_nn'nnGTC
Cuts at: 6636 7494 6636
Size: 858 8072
EaeI y'GGCC_r
Cuts at: 128 380 1397 2666 3002 3686 3719 6515 6689
Size: 252 1017 1269 336 684 33 2796 174
Cuts at: 6689 7080 7107 8452 128
Size: 391 27 1345 606
Fragments arranged by size:
2796 1345 1269 1017 684 606 391 336
252 174 33 27
EagI C'GGCC_G
Cuts at: 6515 6515
Size: 8930
EarI CTCTTCn'nnn_
Cuts at: 1091 1715 2074 2535 2696 2903 2932 3053 3775
Size: 624 359 461 161 207 29 121 722
Cuts at: 3775 3902 4073 4775 4873 5369 5881 5915 6953
Size: 127 171 702 98 496 512 34 1038
Cuts at: 6953 7163 1091
Size: 210 2858
Fragments arranged by size:
2858 1038 722 702 624 512 496 461
359 210 207 171 161 127 121 98
34 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7138 7448 7594 8740 7138
Size: 310 146 1146 7328
Fragments arranged by size:
7328 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1683 5235 5942 1683
Size: 3552 707 4671
Fragments arranged by size:
4671 3552 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 817 940 1127 1271 1555 1624 2055 2327
Size: 491 123 187 144 284 69 431 272
Cuts at: 2327 2333 2770 2819 2968 3100 3493 3568 3673
Size: 6 437 49 149 132 393 75 105
Cuts at: 3673 3790 4297 4366 4723 5140 5329 5446 5476
Size: 117 507 69 357 417 189 117 30
Cuts at: 5476 5554 5584 5695 5786 5848 6755 7187 7934
Size: 78 30 111 91 62 907 432 747
Cuts at: 7934 8202 326
Size: 268 1054
Fragments arranged by size:
1054 907 747 507 491 437 432 431
417 393 357 284 272 268 189 187
149 144 132 123 117 117 111 105
91 78 75 69 69 62 49 30
30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 796 1720 1823 3655 5161 796
Size: 924 103 1832 1506 4565
Fragments arranged by size:
4565 1832 1506 924 103
EcoO109I rG'GnC_Cy
Cuts at: 558 1911 3648 4398 5034 5115 5493 5715 6069
Size: 1353 1737 750 636 81 378 222 354
Cuts at: 6069 558
Size: 3419
Fragments arranged by size:
3419 1737 1353 750 636 378 354 222
81
EcoRI G'AATT_C
Cuts at: 5934 5934
Size: 8930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 261 1440 2440 6949 261
Size: 1179 1000 4509 2242
Fragments arranged by size:
4509 2242 1179 1000
FspI TGC'GCA
Cuts at: 3643 4798 6711 3643
Size: 1155 1913 5862
Fragments arranged by size:
5862 1913 1155
HaeII r_GCGC'y
Cuts at: 435 552 3528 5450 6364 6612 7634 8613 435
Size: 117 2976 1922 914 248 1022 979 752
Fragments arranged by size:
2976 1922 1022 979 914 752 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 378 410 610 642 823 855 1145 1177 1953
Size: 32 200 32 181 32 290 32 776
Cuts at: 1953 1985 2309 2341 2534 2566 3054 3086 4635
Size: 32 324 32 193 32 488 32 1549
Cuts at: 4635 4667 5767 5799 6772 6802 6804 6834 378
Size: 32 1100 32 973 30 2 30 2474
Fragments arranged by size:
2474 1549 1100 973 776 488 324 290
200 193 181 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 486 3171 5793 5871 5969 486
Size: 2685 2622 78 98 3447
Fragments arranged by size:
3447 2685 2622 98 78
HindIII A'AGCT_T
Cuts at: 3355 4523 3355
Size: 1168 7762
KasI G'GCGC_C
Cuts at: 6360 6608 6360
Size: 248 8682
KpnI G_GTAC'C
Cuts at: 878 5950 878
Size: 5072 3858
MfeI C'AATT_G
Cuts at: 701 701
Size: 8930
MluI A'CGCG_T
Cuts at: 8363 8363
Size: 8930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 5810 7601 7785 140
Size: 5670 1791 184 1285
Fragments arranged by size:
5670 1791 1285 184
MscI TGG'CCA
Cuts at: 130 382 1399 2668 3004 6691 130
Size: 252 1017 1269 336 3687 2369
Fragments arranged by size:
3687 2369 1269 1017 336 252
MslI CAynn'nnrTG
Cuts at: 382 1597 2587 4136 5090 6919 7046 382
Size: 1215 990 1549 954 1829 127 2266
Fragments arranged by size:
2266 1829 1549 1215 990 954 127
MspA1I CmG'CkG
Cuts at: 1455 1542 2196 2469 3429 3921 5004 5130 5526
Size: 87 654 273 960 492 1083 126 396
Cuts at: 5526 6041 6355 6715 7728 7973 1455
Size: 515 314 360 1013 245 2412
Fragments arranged by size:
2412 1083 1013 960 654 515 492 396
360 314 273 245 126 87
NaeI GCC'GGC
Cuts at: 1890 7111 1890
Size: 5221 3709
NarI GG'CG_CC
Cuts at: 6361 6609 6361
Size: 248 8682
NcoI C'CATG_G
Cuts at: 2168 2168
Size: 8930
NgoMIV G'CCGG_C
Cuts at: 1888 7109 1888
Size: 5221 3709
NsiI A_TGCA'T
Cuts at: 1190 1190
Size: 8930
NspI r_CATG'y
Cuts at: 1596 1734 3028 3709 4114 4330 5983 6483 7390
Size: 138 1294 681 405 216 1653 500 907
Cuts at: 7390 1596
Size: 3136
Fragments arranged by size:
3136 1653 1294 907 681 500 405 216
138
PciI A'CATG_T
Cuts at: 1592 4326 7386 1592
Size: 2734 3060 3136
Fragments arranged by size:
3136 3060 2734
PflMI CCAn_nnn'nTGG
Cuts at: 1455 2529 4695 5562 5718 1455
Size: 1074 2166 867 156 4667
Fragments arranged by size:
4667 2166 1074 867 156
PfoI T'CCnGG_A
Cuts at: 1715 3116 5237 5354 5711 1715
Size: 1401 2121 117 357 4934
Fragments arranged by size:
4934 2121 1401 357 117
PmeI GTTT'AAAC
Cuts at: 5929 5929
Size: 8930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1627 1659 1717 1749 3613 3645 5219 5251 8069
Size: 32 58 32 1864 32 1574 32 2818
Cuts at: 8069 8101 1627
Size: 32 2456
Fragments arranged by size:
2818 2456 1864 1574 58 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 1911 3648 4398 5115 5493 5715 1911
Size: 1737 750 717 378 222 5126
Fragments arranged by size:
5126 1737 750 717 378 222
PshAI GACnn'nnGTC
Cuts at: 217 217
Size: 8930
PstI C_TGCA'G
Cuts at: 1296 2236 3152 3434 3752 4121 4313 5165 5501
Size: 940 916 282 318 369 192 852 336
Cuts at: 5501 5977 6662 1296
Size: 476 685 3564
Fragments arranged by size:
3564 940 916 852 685 476 369 336
318 282 192
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8930
PvuII CAG'CTG
Cuts at: 1455 1542 2196 2469 3429 5004 5130 5526 6355
Size: 87 654 273 960 1575 126 396 829
Cuts at: 6355 6715 1455
Size: 360 3670
Fragments arranged by size:
3670 1575 960 829 654 396 360 273
126 87
RsrII CG'GwC_CG
Cuts at: 7125 7125
Size: 8930
SacI G_AGCT'C
Cuts at: 1685 5237 5944 1685
Size: 3552 707 4671
Fragments arranged by size:
4671 3552 707
SalI G'TCGA_C
Cuts at: 5967 5967
Size: 8930
SapI GCTCTTCn'nnn_
Cuts at: 2696 5881 6953 7163 2696
Size: 3185 1072 210 4463
Fragments arranged by size:
4463 3185 1072 210
SbfI CC_TGCA'GG
Cuts at: 3152 5977 3152
Size: 2825 6105
ScaI AGT'ACT
Cuts at: 691 1480 8447 691
Size: 789 6967 1174
Fragments arranged by size:
6967 1174 789
SexAI A'CCwGG_T
Cuts at: 5717 5717
Size: 8930
SfcI C'TryA_G
Cuts at: 33 1109 1292 2232 3148 3430 3667 3748 4117
Size: 1076 183 940 916 282 237 81 369
Cuts at: 4117 4189 4309 5046 5161 5497 5973 6658 7651
Size: 72 120 737 115 336 476 685 993
Cuts at: 7651 7842 33
Size: 191 1121
Fragments arranged by size:
1121 1076 993 940 916 737 685 476
369 336 282 237 191 183 120 115
81 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 3505 5389 3505
Size: 1884 7046
SfoI GGC'GCC
Cuts at: 6362 6610 6362
Size: 248 8682
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8930
SmaI CCC'GGG
Cuts at: 3505 3541 5952 3505
Size: 36 2411 6483
Fragments arranged by size:
6483 2411 36
SmlI C'TyrA_G
Cuts at: 2103 2968 2989 3305 4023 4360 4807 4960 5068
Size: 865 21 316 718 337 447 153 108
Cuts at: 5068 6085 7000 7492 7754 2103
Size: 1017 915 492 262 3279
Fragments arranged by size:
3279 1017 915 865 718 492 447 337
316 262 153 108 21
SpeI A'CTAG_T
Cuts at: 67 4008 67
Size: 3941 4989
SphI G_CATG'C
Cuts at: 3709 5983 6483 3709
Size: 2274 500 6156
Fragments arranged by size:
6156 2274 500
SspI AAT'ATT
Cuts at: 3979 5700 3979
Size: 1721 7209
StuI AGG'CCT
Cuts at: 802 802
Size: 8930
StyI C'CwwG_G
Cuts at: 1321 1739 2168 2402 3005 3098 3417 3842 3902
Size: 418 429 234 603 93 319 425 60
Cuts at: 3902 4178 4205 6064 6418 1321
Size: 276 27 1859 354 3833
Fragments arranged by size:
3833 1859 603 429 425 418 354 319
276 234 93 60 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 313 1378 2395 2731 5071 5287 6513 6646 7105
Size: 1065 1017 336 2340 216 1226 133 459
Cuts at: 7105 7314 313
Size: 209 1929
Fragments arranged by size:
2340 1929 1226 1065 1017 459 336 216
209 133
TatI w'GTAC_w
Cuts at: 416 689 1376 1478 1616 2564 8445 416
Size: 273 687 102 138 948 5881 901
Fragments arranged by size:
5881 948 901 687 273 138 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 559 591 1528 1560 1711 1743 8447 8479 559
Size: 32 937 32 151 32 6704 32 1010
Fragments arranged by size:
6704 1010 937 151 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 334 2058 3166 6727 334
Size: 1724 1108 3561 2537
Fragments arranged by size:
3561 2537 1724 1108
XbaI T'CTAG_A
Cuts at: 928 3261 3873 5961 928
Size: 2333 612 2088 3897
Fragments arranged by size:
3897 2333 2088 612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 792 885 1128 2389 2703 4101 4293 5304 5451
Size: 93 243 1261 314 1398 192 1011 147
Cuts at: 5451 6425 792
Size: 974 3297
Fragments arranged by size:
3297 1398 1261 1011 974 314 243 192
147 93
XhoI C'TCGA_G
Cuts at: 4023 4023
Size: 8930
XmaI C'CCGG_G
Cuts at: 3503 3539 5950 3503
Size: 36 2411 6483
Fragments arranged by size:
6483 2411 36
XmnI GAAnn'nnTTC
Cuts at: 2242 2242
Size: 8930
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI ApaI AscI BmgBI
BmtI BsiWI BsmBI BstZ17I CspCI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI PacI PasI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SgrAI SnaBI
SrfI SwaI ZraI