Restriction map for
pF1KE2590
(Circular) (Six-base) MAPSORT of: pF1KE2590.seq Check: 3691 from: 1 to: 5498
pF1KE2590 5498 bp
With 182 enzymes: SgfI *
September 30, 2015 09:49 ..
AccI GT'mk_AC
Cuts at: 2536 2536
Size: 5498
Acc65I G'GTAC_C
Cuts at: 1751 2435 2514 1751
Size: 684 79 4735
Fragments arranged by size:
4735 684 79
AclI AA'CG_TT
Cuts at: 922 5338 922
Size: 4416 1082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 957 1756 1903 1951 2112 2114 3323 3755
Size: 350 799 147 48 161 2 1209 432
Cuts at: 3755 4502 4770 607
Size: 747 268 1335
Fragments arranged by size:
1335 1209 799 747 432 350 268 161
147 48 2
AflIII A'CryG_T
Cuts at: 854 2850 3954 4931 854
Size: 1996 1104 977 1421
Fragments arranged by size:
1996 1421 1104 977
AgeI A'CCGG_T
Cuts at: 3886 3886
Size: 5498
AhdI GACnn_n'nnGTC
Cuts at: 2337 2337
Size: 5498
AlwNI CAG_nnn'CTG
Cuts at: 1713 2281 2803 4370 1713
Size: 568 522 1567 2841
Fragments arranged by size:
2841 1567 568 522
ApaLI G'TGCA_C
Cuts at: 4268 4268
Size: 5498
ApoI r'AATT_y
Cuts at: 1305 1386 2502 4851 5107 1305
Size: 81 1116 2349 256 1696
Fragments arranged by size:
2349 1696 1116 256 81
AseI AT'TA_AT
Cuts at: 20 20
Size: 5498
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
AvaI C'yCGr_G
Cuts at: 604 1766 2320 2518 604
Size: 1162 554 198 3584
Fragments arranged by size:
3584 1162 554 198
BamHI G'GATC_C
Cuts at: 513 639 2523 513
Size: 126 1884 3488
Fragments arranged by size:
3488 1884 126
BanI G'GyrC_C
Cuts at: 282 1743 1751 2435 2514 2928 3176 3211 282
Size: 1461 8 684 79 414 248 35 2569
Fragments arranged by size:
2569 1461 684 414 248 79 35 8
BanII G_rGCy'C
Cuts at: 788 868 2512 3542 788
Size: 80 1644 1030 2744
Fragments arranged by size:
2744 1644 1030 80
BbeI G_GCGC'C
Cuts at: 1747 2932 3180 1747
Size: 1185 248 4065
Fragments arranged by size:
4065 1185 248
BbsI GAAGACnn'nnnn_
Cuts at: 1068 1127 1226 2064 1068
Size: 59 99 838 4502
Fragments arranged by size:
4502 838 99 59
BbvCI CC'TCA_GC
Cuts at: 1919 2356 1919
Size: 437 5061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1729 1763 2025 2059 1729
Size: 34 262 34 5168
Fragments arranged by size:
5168 262 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3396 4157 5498 3396
Size: 761 1341 3396
Fragments arranged by size:
3396 1341 761
BclI T'GATC_A
Cuts at: 131 3018 131
Size: 2887 2611
BglI GCCn_nnn'nGGC
Cuts at: 163 163
Size: 5498
BglII A'GATC_T
Cuts at: 3013 5050 3013
Size: 2037 3461
BlpI GC'TnA_GC
Cuts at: 2610 2610
Size: 5498
Bme1580I G_kGCm'C
Cuts at: 287 3123 3216 4272 287
Size: 2836 93 1056 1513
Fragments arranged by size:
2836 1513 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 639 1454 1614 1938 2710 2791 3126 5270 639
Size: 815 160 324 772 81 335 2144 867
Fragments arranged by size:
2144 867 815 772 335 324 160 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1653 3865 1653
Size: 2212 3286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2090 2419 2042
Size: 48 329 5121
Fragments arranged by size:
5121 329 48
Bpu10I CC'TnA_GC
Cuts at: 490 794 1919 2356 2431 490
Size: 304 1125 437 75 3557
Fragments arranged by size:
3557 1125 437 304 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 2674 3553 4045 4343 868
Size: 1806 879 492 298 2023
Fragments arranged by size:
2023 1806 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1508 5202 1508
Size: 3694 1804
BsaAI yAC'GTr
Cuts at: 1854 2328 2851 1854
Size: 474 523 4501
Fragments arranged by size:
4501 523 474
BsaBI GATnn'nnATC
Cuts at: 6 3012 5049 6
Size: 3006 2037 455
Fragments arranged by size:
3006 2037 455
BsaHI Gr'CG_yC
Cuts at: 1744 2929 3177 3879 5375 1744
Size: 1185 248 702 1496 1867
Fragments arranged by size:
1867 1496 1185 702 248
BsaWI w'CCGG_w
Cuts at: 839 1586 2687 3208 3886 4160 4307 4718 839
Size: 747 1101 521 678 274 147 411 1619
Fragments arranged by size:
1619 1101 747 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 345 375 345
Size: 30 5468
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 366 369 989 1023 1219 1410 1699 2340 2418
Size: 3 620 34 196 191 289 641 78
Cuts at: 2418 366
Size: 3446
Fragments arranged by size:
3446 641 620 289 196 191 78 34
3
BseYI C'CCAG_C
Cuts at: 144 166 298 521 1179 1480 2229 2923 3681
Size: 22 132 223 658 301 749 694 758
Cuts at: 3681 4258 4754 144
Size: 577 496 888
Fragments arranged by size:
888 758 749 694 658 577 496 301
223 132 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 1112 278
Size: 834 4664
BsiEI CG_ry'CG
Cuts at: 104 3040 3086 4294 104
Size: 2936 46 1208 1308
Fragments arranged by size:
2936 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 424 2327 2512 3290 3480 4272 424
Size: 1903 185 778 190 792 1650
Fragments arranged by size:
1903 1650 792 778 190 185
Bsp1286I G_dGCh'C
Cuts at: 287 424 788 868 2327 2512 3123 3216 3290
Size: 137 364 80 1459 185 611 93 74
Cuts at: 3290 3480 3542 4272 287
Size: 190 62 730 1513
Fragments arranged by size:
1513 1459 730 611 364 190 185 137
93 80 74 62
BspEI T'CCGG_A
Cuts at: 1586 4718 1586
Size: 3132 2366
BspHI T'CATG_A
Cuts at: 1907 2201 4674 1907
Size: 294 2473 2731
Fragments arranged by size:
2731 2473 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 582 1377 2548 2762 3064 3445 582
Size: 795 1171 214 302 381 2635
Fragments arranged by size:
2635 1171 795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3790 3844 5328 5493 50
Size: 3740 54 1484 165 55
Fragments arranged by size:
3740 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2417 3410 2417
Size: 993 4505
BsrFI r'CCGG_y
Cuts at: 3496 3677 3886 3496
Size: 181 209 5108
Fragments arranged by size:
5108 209 181
BssSI C'ACGA_G
Cuts at: 3769 4127 3769
Size: 358 5140
BstAPI GCAn_nnn'nTGC
Cuts at: 2875 2875
Size: 5498
BstBI TT'CG_AA
Cuts at: 3859 3859
Size: 5498
BstYI r'GATC_y
Cuts at: 1 513 639 1230 2144 2523 2990 3013 3348
Size: 512 126 591 914 379 467 23 335
Cuts at: 3348 3594 4606 4692 4704 5050 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 914 591 512 467 449 379 346
335 246 126 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3448 3629 3792 3448
Size: 181 163 5154
Fragments arranged by size:
5154 181 163
BtsI GCAGTG_nn'
Cuts at: 455 672 2741 455
Size: 217 2069 3212
Fragments arranged by size:
3212 2069 217
DraI TTT'AAA
Cuts at: 2497 2497
Size: 5498
DrdI GACnn_nn'nnGTC
Cuts at: 3204 4062 3204
Size: 858 4640
EaeI y'GGCC_r
Cuts at: 3083 3257 3648 3675 5020 3083
Size: 174 391 27 1345 3561
Fragments arranged by size:
3561 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3083 3083
Size: 5498
EarI CTCTTCn'nnn_
Cuts at: 1332 1531 1603 1804 2350 3521 3731 1332
Size: 199 72 201 546 1171 210 3099
Fragments arranged by size:
3099 1171 546 210 201 199 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1223 2353 3706 4016 4162 5308 1223
Size: 1130 1353 310 146 1146 1413
Fragments arranged by size:
1413 1353 1146 1130 310 146
EcoICRI GAG'CTC
Cuts at: 2510 2510
Size: 5498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 957 1756 1903 1951 2042 2090 2112 2114
Size: 350 799 147 48 91 48 22 2
Cuts at: 2114 2419 3323 3755 4502 4770 607
Size: 305 904 432 747 268 1335
Fragments arranged by size:
1335 904 799 747 432 350 305 268
147 91 48 48 22 2
EcoO109I rG'GnC_Cy
Cuts at: 468 830 1448 2637 468
Size: 362 618 1189 3329
Fragments arranged by size:
3329 1189 618 362
EcoRI G'AATT_C
Cuts at: 2502 2502
Size: 5498
EcoRV GAT'ATC
Cuts at: 257 257
Size: 5498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 576 1317 3517 576
Size: 741 2200 2557
Fragments arranged by size:
2557 2200 741
FspI TGC'GCA
Cuts at: 3279 3279
Size: 5498
HaeII r_GCGC'y
Cuts at: 1713 1747 2088 2932 3180 4202 5181 1713
Size: 34 341 844 248 1022 979 2030
Fragments arranged by size:
2030 1022 979 844 341 248 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 562 594 685 717 2159 2191 2323
Size: 32 289 32 91 32 1442 32 132
Cuts at: 2323 2355 2464 2496 3340 3370 3372 3402 241
Size: 32 109 32 844 30 2 30 2337
Fragments arranged by size:
2337 1442 844 289 132 109 91 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1719 2537 1719
Size: 818 4680
KasI G'GCGC_C
Cuts at: 1743 2928 3176 1743
Size: 1185 248 4065
Fragments arranged by size:
4065 1185 248
KpnI G_GTAC'C
Cuts at: 1755 2439 2518 1755
Size: 684 79 4735
Fragments arranged by size:
4735 684 79
MluI A'CGCG_T
Cuts at: 4931 4931
Size: 5498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1404 1868 4169 4353 1404
Size: 464 2301 184 2549
Fragments arranged by size:
2549 2301 464 184
MscI TGG'CCA
Cuts at: 3259 3259
Size: 5498
MslI CAynn'nnrTG
Cuts at: 112 3487 3614 112
Size: 3375 127 1996
Fragments arranged by size:
3375 1996 127
MspA1I CmG'CkG
Cuts at: 148 2307 2609 2923 3283 4296 4541 148
Size: 2159 302 314 360 1013 245 1105
Fragments arranged by size:
2159 1105 1013 360 314 302 245
NaeI GCC'GGC
Cuts at: 3679 3679
Size: 5498
NarI GG'CG_CC
Cuts at: 1744 2929 3177 1744
Size: 1185 248 4065
Fragments arranged by size:
4065 1185 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5498
NgoMIV G'CCGG_C
Cuts at: 3677 3677
Size: 5498
NspI r_CATG'y
Cuts at: 934 2551 3051 3958 934
Size: 1617 500 907 2474
Fragments arranged by size:
2474 1617 907 500
PasI CC'CwG_GG
Cuts at: 833 2235 833
Size: 1402 4096
PciI A'CATG_T
Cuts at: 3954 3954
Size: 5498
PflMI CCAn_nnn'nTGG
Cuts at: 547 1626 1713 1970 547
Size: 1079 87 257 4075
Fragments arranged by size:
4075 1079 257 87
PmeI GTTT'AAAC
Cuts at: 2497 2497
Size: 5498
PmlI CAC'GTG
Cuts at: 2328 2328
Size: 5498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 4637
Size: 32 5466
PpuMI rG'GwC_Cy
Cuts at: 468 1448 468
Size: 980 4518
PspXI vC'TCGA_Gb
Cuts at: 604 2320 604
Size: 1716 3782
PstI C_TGCA'G
Cuts at: 153 1133 2545 3230 153
Size: 980 1412 685 2421
Fragments arranged by size:
2421 1412 980 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5498
PvuII CAG'CTG
Cuts at: 148 2923 3283 148
Size: 2775 360 2363
Fragments arranged by size:
2775 2363 360
RsrII CG'GwC_CG
Cuts at: 3693 3693
Size: 5498
SacI G_AGCT'C
Cuts at: 2512 2512
Size: 5498
SalI G'TCGA_C
Cuts at: 2535 2535
Size: 5498
SanDI GG'GwC_CC
Cuts at: 1448 1448
Size: 5498
SapI GCTCTTCn'nnn_
Cuts at: 1804 3521 3731 1804
Size: 1717 210 3571
Fragments arranged by size:
3571 1717 210
SbfI CC_TGCA'GG
Cuts at: 2545 2545
Size: 5498
ScaI AGT'ACT
Cuts at: 1807 5015 1807
Size: 3208 2290
SexAI A'CCwGG_T
Cuts at: 2369 2369
Size: 5498
SfcI C'TryA_G
Cuts at: 33 149 1129 2541 3226 4219 4410 33
Size: 116 980 1412 685 993 191 1121
Fragments arranged by size:
1412 1121 993 980 685 191 116
SfoI GGC'GCC
Cuts at: 1745 2930 3178 1745
Size: 1185 248 4065
Fragments arranged by size:
4065 1185 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
SmaI CCC'GGG
Cuts at: 1768 2520 1768
Size: 752 4746
SmlI C'TyrA_G
Cuts at: 604 847 2320 2653 3568 4060 4322 604
Size: 243 1473 333 915 492 262 1780
Fragments arranged by size:
1780 1473 915 492 333 262 243
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5498
SphI G_CATG'C
Cuts at: 2551 3051 2551
Size: 500 4998
StyI C'CwwG_G
Cuts at: 107 1509 1964 2443 2632 2986 107
Size: 1402 455 479 189 354 2619
Fragments arranged by size:
2619 1402 479 455 354 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 312 1882 3081 3214 3673 3882 312
Size: 1570 1199 133 459 209 1928
Fragments arranged by size:
1928 1570 1199 459 209 133
TatI w'GTAC_w
Cuts at: 1805 5013 1805
Size: 3208 2290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 345 377 1090 1122 2270 2302 5015 5047 345
Size: 32 713 32 1148 32 2713 32 796
Fragments arranged by size:
2713 1148 796 713 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3295 3295
Size: 5498
XbaI T'CTAG_A
Cuts at: 1227 2529 1227
Size: 1302 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2993 2993
Size: 5498
XhoI C'TCGA_G
Cuts at: 604 2320 604
Size: 1716 3782
XmaI C'CCGG_G
Cuts at: 1766 2518 1766
Size: 752 4746
XmnI GAAnn'nnTTC
Cuts at: 1223 1223
Size: 5498
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PshAI
PsiI PspOMI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI