Restriction map for
pF1KE2589
(Circular) (Six-base) MAPSORT of: pF1KE2589.seq Check: 1551 from: 1 to: 6029
pF1KE2589 6029 bp
With 182 enzymes: SgfI *
October 20, 2015 16:07 ..
AccI GT'mk_AC
Cuts at: 3067 3067
Size: 6029
Acc65I G'GTAC_C
Cuts at: 1475 3045 1475
Size: 1570 4459
AclI AA'CG_TT
Cuts at: 5869 5869
Size: 6029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1578 1732 2029 2394 3854 4286 5033 5301 1578
Size: 154 297 365 1460 432 747 268 2306
Fragments arranged by size:
2306 1460 747 432 365 297 268 154
AfeI AGC'GCT
Cuts at: 2990 2990
Size: 6029
AflIII A'CryG_T
Cuts at: 2570 3381 4485 5462 2570
Size: 811 1104 977 3137
Fragments arranged by size:
3137 1104 977 811
AgeI A'CCGG_T
Cuts at: 4417 4417
Size: 6029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 6029
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1483 1515 1483
Size: 32 5997
AlwNI CAG_nnn'CTG
Cuts at: 192 574 621 726 949 1198 1710 2946 3334
Size: 382 47 105 223 249 512 1236 388
Cuts at: 3334 4901 192
Size: 1567 1320
Fragments arranged by size:
1567 1320 1236 512 388 382 249 223
105 47
ApaI G_GGCC'C
Cuts at: 2190 2702 2190
Size: 512 5517
ApaLI G'TGCA_C
Cuts at: 421 4799 421
Size: 4378 1651
ApoI r'AATT_y
Cuts at: 559 1879 3033 5382 5638 559
Size: 1320 1154 2349 256 950
Fragments arranged by size:
2349 1320 1154 950 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6029
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6029
AvaI C'yCGr_G
Cuts at: 535 1103 3049 535
Size: 568 1946 3515
Fragments arranged by size:
3515 1946 568
BamHI G'GATC_C
Cuts at: 3054 3054
Size: 6029
BanI G'GyrC_C
Cuts at: 451 1475 1733 3045 3459 3707 3742 451
Size: 1024 258 1312 414 248 35 2738
Fragments arranged by size:
2738 1312 1024 414 258 248 35
BanII G_rGCy'C
Cuts at: 137 700 994 1365 2006 2190 2702 2717 2825
Size: 563 294 371 641 184 512 15 108
Cuts at: 2825 2981 3043 4073 137
Size: 156 62 1030 2093
Fragments arranged by size:
2093 1030 641 563 512 371 294 184
156 108 62 15
BbeI G_GCGC'C
Cuts at: 1737 3463 3711 1737
Size: 1726 248 4055
Fragments arranged by size:
4055 1726 248
BbsI GAAGACnn'nnnn_
Cuts at: 211 1512 1691 211
Size: 1301 179 4549
Fragments arranged by size:
4549 1301 179
BbvCI CC'TCA_GC
Cuts at: 1985 1985
Size: 6029
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1917 1951 90
Size: 34 1793 34 4168
Fragments arranged by size:
4168 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1332 3927 4688 6029 1332
Size: 2595 761 1341 1332
Fragments arranged by size:
2595 1341 1332 761
BclI T'GATC_A
Cuts at: 3549 3549
Size: 6029
BfrBI ATG'CAT
Cuts at: 2750 2750
Size: 6029
BglI GCCn_nnn'nGGC
Cuts at: 2374 2374
Size: 6029
BglII A'GATC_T
Cuts at: 841 2456 3544 5581 841
Size: 1615 1088 2037 1289
Fragments arranged by size:
2037 1615 1289 1088
BlpI GC'TnA_GC
Cuts at: 773 1781 2711 3141 773
Size: 1008 930 430 3661
Fragments arranged by size:
3661 1008 930 430
Bme1580I G_kGCm'C
Cuts at: 425 821 899 2190 2502 2702 3654 3747 4803
Size: 396 78 1291 312 200 952 93 1056
Cuts at: 4803 425
Size: 1651
Fragments arranged by size:
1651 1291 1056 952 396 312 200 93
78
BmgBI CAC'GTC
Cuts at: 900 1979 900
Size: 1079 4950
BmrI ACTGGGnnnn_n'
Cuts at: 734 931 1812 1941 3241 3322 3657 5801 734
Size: 197 881 129 1300 81 335 2144 962
Fragments arranged by size:
2144 1300 962 881 335 197 129 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4396 4396
Size: 6029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 978 1158 1338 1349 1791 2071 2968 3034 978
Size: 180 180 11 442 280 897 66 3973
Fragments arranged by size:
3973 897 442 280 180 180 66 11
Bpu10I CC'TnA_GC
Cuts at: 1485 1985 1485
Size: 500 5529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 1905 3205 4084 4576 4874 866
Size: 1039 1300 879 492 298 2021
Fragments arranged by size:
2021 1300 1039 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5733 5733
Size: 6029
BsaAI yAC'GTr
Cuts at: 402 1831 3382 402
Size: 1429 1551 3049
Fragments arranged by size:
3049 1551 1429
BsaBI GATnn'nnATC
Cuts at: 6 2377 3543 5580 6
Size: 2371 1166 2037 455
Fragments arranged by size:
2371 2037 1166 455
BsaHI Gr'CG_yC
Cuts at: 1734 3460 3708 4410 5906 1734
Size: 1726 248 702 1496 1857
Fragments arranged by size:
1857 1726 1496 702 248
BsaWI w'CCGG_w
Cuts at: 371 2121 2285 3218 3739 4417 4691 4838 5249
Size: 1750 164 933 521 678 274 147 411
Cuts at: 5249 371
Size: 1151
Fragments arranged by size:
1750 1151 933 678 521 411 274 164
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1312 1342 2038 2068 1312
Size: 30 696 30 5273
Fragments arranged by size:
5273 696 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 178 1161 1678 1681 2986 2989 163
Size: 15 983 517 3 1305 3 3203
Fragments arranged by size:
3203 1305 983 517 15 3 3
BseYI C'CCAG_C
Cuts at: 192 518 1441 2383 2694 3454 4212 4789 5285
Size: 326 923 942 311 760 758 577 496
Cuts at: 5285 192
Size: 936
Fragments arranged by size:
942 936 923 760 758 577 496 326
311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1954 2291 2503 935
Size: 1019 337 212 4461
Fragments arranged by size:
4461 1019 337 212
BsiEI CG_ry'CG
Cuts at: 104 115 3571 3617 4825 104
Size: 11 3456 46 1208 1308
Fragments arranged by size:
3456 1308 1208 46 11
BsiHKAI G_wGCw'C
Cuts at: 137 182 425 994 1365 1486 1787 2717 2825
Size: 45 243 569 371 121 301 930 108
Cuts at: 2825 3043 3821 4011 4803 137
Size: 218 778 190 792 1363
Fragments arranged by size:
1363 930 792 778 569 371 301 243
218 190 121 108 45
BsmI GAATG_Cn'
Cuts at: 777 1590 2370 777
Size: 813 780 4436
Fragments arranged by size:
4436 813 780
BsmBI CGTCTCn'nnnn_
Cuts at: 906 906
Size: 6029
Bsp1286I G_dGCh'C
Cuts at: 137 182 425 700 821 899 994 1365 1486
Size: 45 243 275 121 78 95 371 121
Cuts at: 1486 1787 2006 2190 2502 2702 2717 2825 2981
Size: 301 219 184 312 200 15 108 156
Cuts at: 2981 3043 3654 3747 3821 4011 4073 4803 137
Size: 62 611 93 74 190 62 730 1363
Fragments arranged by size:
1363 730 611 371 312 301 275 243
219 200 190 184 156 121 121 108
95 93 78 74 62 62 45 15
BspEI T'CCGG_A
Cuts at: 2285 5249 2285
Size: 2964 3065
BspHI T'CATG_A
Cuts at: 5205 5205
Size: 6029
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1253 1766 2453 3079 3293 3595 3976 1253
Size: 513 687 626 214 302 381 3306
Fragments arranged by size:
3306 687 626 513 381 302 214
BsrBI CCG'CTC
Cuts at: 50 273 702 2907 4321 4375 5859 6024 50
Size: 223 429 2205 1414 54 1484 165 55
Fragments arranged by size:
2205 1484 1414 429 223 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1146 1346 1383 3941 1146
Size: 200 37 2558 3234
Fragments arranged by size:
3234 2558 200 37
BsrFI r'CCGG_y
Cuts at: 4027 4208 4417 4027
Size: 181 209 5639
Fragments arranged by size:
5639 209 181
BsrGI T'GTAC_A
Cuts at: 1451 1451
Size: 6029
BssSI C'ACGA_G
Cuts at: 424 691 2359 4300 4658 424
Size: 267 1668 1941 358 1795
Fragments arranged by size:
1941 1795 1668 358 267
BstAPI GCAn_nnn'nTGC
Cuts at: 495 2756 3406 495
Size: 2261 650 3118
Fragments arranged by size:
3118 2261 650
BstBI TT'CG_AA
Cuts at: 4390 4390
Size: 6029
BstEII G'GTnAC_C
Cuts at: 1800 1800
Size: 6029
BstYI r'GATC_y
Cuts at: 1 841 1700 1721 2456 3054 3521 3544 3879
Size: 840 859 21 735 598 467 23 335
Cuts at: 3879 4125 5137 5223 5235 5581 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 859 840 735 598 467 449 346
335 246 86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 161 1212 161
Size: 1051 4978
BtgI C'CryG_G
Cuts at: 1997 2246 2981 1997
Size: 249 735 5045
Fragments arranged by size:
5045 735 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3979 4160 4323 3979
Size: 181 163 5685
Fragments arranged by size:
5685 181 163
BtsI GCAGTG_nn'
Cuts at: 447 504 622 635 1431 1959 2107 3272 447
Size: 57 118 13 796 528 148 1165 3204
Fragments arranged by size:
3204 1165 796 528 148 118 57 13
ClaI AT'CG_AT
Cuts at: 665 665
Size: 6029
DraI TTT'AAA
Cuts at: 3028 3028
Size: 6029
DrdI GACnn_nn'nnGTC
Cuts at: 598 3735 4593 598
Size: 3137 858 2034
Fragments arranged by size:
3137 2034 858
EaeI y'GGCC_r
Cuts at: 112 605 1082 1994 2249 2375 2591 2933 3614
Size: 493 477 912 255 126 216 342 681
Cuts at: 3614 3788 4179 4206 5551 112
Size: 174 391 27 1345 590
Fragments arranged by size:
1345 912 681 590 493 477 391 342
255 216 174 126 27
EagI C'GGCC_G
Cuts at: 112 3614 112
Size: 3502 2527
EarI CTCTTCn'nnn_
Cuts at: 1212 1673 2479 2840 4052 4262 1212
Size: 461 806 361 1212 210 2979
Fragments arranged by size:
2979 1212 806 461 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1714 4237 4547 4693 5839 1714
Size: 2523 310 146 1146 1904
Fragments arranged by size:
2523 1904 1146 310 146
EcoICRI GAG'CTC
Cuts at: 135 992 1363 2715 2823 3041 135
Size: 857 371 1352 108 218 3123
Fragments arranged by size:
3123 1352 857 371 218 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 978 1158 1338 1349 1578 1732 1791 2029 2071
Size: 180 180 11 229 154 59 238 42
Cuts at: 2071 2394 2968 3034 3854 4286 5033 5301 978
Size: 323 574 66 820 432 747 268 1706
Fragments arranged by size:
1706 820 747 574 432 323 268 238
229 180 180 154 66 59 42 11
EcoNI CCTnn'n_nnAGG
Cuts at: 160 1576 1663 2638 160
Size: 1416 87 975 3551
Fragments arranged by size:
3551 1416 975 87
EcoO109I rG'GnC_Cy
Cuts at: 791 1069 1399 2187 2442 2643 2698 2699 3168
Size: 278 330 788 255 201 55 1 469
Cuts at: 3168 791
Size: 3652
Fragments arranged by size:
3652 788 469 330 278 255 201 55
1
EcoRI G'AATT_C
Cuts at: 3033 3033
Size: 6029
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 863 2424 4048 863
Size: 1561 1624 2844
Fragments arranged by size:
2844 1624 1561
FspI TGC'GCA
Cuts at: 2398 3810 2398
Size: 1412 4617
HaeII r_GCGC'y
Cuts at: 156 1737 2992 3463 3711 4733 5712 156
Size: 1581 1255 471 248 1022 979 473
Fragments arranged by size:
1581 1255 1022 979 473 471 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 536 568 826 858 884 916 1511 1543 1843
Size: 32 258 32 26 32 595 32 300
Cuts at: 1843 1875 2267 2299 2738 2770 3871 3901 3903
Size: 32 392 32 439 32 1101 30 2
Cuts at: 3903 3933 536
Size: 30 2632
Fragments arranged by size:
2632 1101 595 439 392 300 258 32
32 32 32 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 3068 3068
Size: 6029
HindIII A'AGCT_T
Cuts at: 1791 1791
Size: 6029
KasI G'GCGC_C
Cuts at: 1733 3459 3707 1733
Size: 1726 248 4055
Fragments arranged by size:
4055 1726 248
KpnI G_GTAC'C
Cuts at: 1479 3049 1479
Size: 1570 4459
MluI A'CGCG_T
Cuts at: 5462 5462
Size: 6029
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4700 4884 4700
Size: 184 5845
MscI TGG'CCA
Cuts at: 1084 1996 2251 2377 2593 2935 3790 1084
Size: 912 255 126 216 342 855 3323
Fragments arranged by size:
3323 912 855 342 255 216 126
MslI CAynn'nnrTG
Cuts at: 418 607 2363 4018 4145 418
Size: 189 1756 1655 127 2302
Fragments arranged by size:
2302 1756 1655 189 127
MspA1I CmG'CkG
Cuts at: 1121 1245 1562 2267 2553 2983 3140 3454 3814
Size: 124 317 705 286 430 157 314 360
Cuts at: 3814 4827 5072 1121
Size: 1013 245 2078
Fragments arranged by size:
2078 1013 705 430 360 317 314 286
245 157 124
NaeI GCC'GGC
Cuts at: 4210 4210
Size: 6029
NarI GG'CG_CC
Cuts at: 1734 3460 3708 1734
Size: 1726 248 4055
Fragments arranged by size:
4055 1726 248
NcoI C'CATG_G
Cuts at: 1997 1997
Size: 6029
NgoMIV G'CCGG_C
Cuts at: 4208 4208
Size: 6029
NsiI A_TGCA'T
Cuts at: 2752 2752
Size: 6029
NspI r_CATG'y
Cuts at: 291 1500 2574 2754 3082 3582 4489 291
Size: 1209 1074 180 328 500 907 1831
Fragments arranged by size:
1831 1209 1074 907 500 328 180
PciI A'CATG_T
Cuts at: 2570 4485 2570
Size: 1915 4114
PflMI CCAn_nnn'nTGG
Cuts at: 248 862 248
Size: 614 5415
PfoI T'CCnGG_A
Cuts at: 1703 2063 3011 1703
Size: 360 948 4721
Fragments arranged by size:
4721 948 360
PmeI GTTT'AAAC
Cuts at: 3028 3028
Size: 6029
PmlI CAC'GTG
Cuts at: 402 1831 402
Size: 1429 4600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1140 1172 1903 1935 5168 5200 1140
Size: 32 731 32 3233 32 1969
Fragments arranged by size:
3233 1969 731 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 791 2442 2643 791
Size: 1651 201 4177
Fragments arranged by size:
4177 1651 201
PspOMI G'GGCC_C
Cuts at: 2186 2698 2186
Size: 512 5517
PstI C_TGCA'G
Cuts at: 488 500 617 2915 3076 3761 488
Size: 12 117 2298 161 685 2756
Fragments arranged by size:
2756 2298 685 161 117 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6029
PvuII CAG'CTG
Cuts at: 1121 1245 1562 2553 3454 3814 1121
Size: 124 317 991 901 360 3336
Fragments arranged by size:
3336 991 901 360 317 124
RsrII CG'GwC_CG
Cuts at: 4224 4224
Size: 6029
SacI G_AGCT'C
Cuts at: 137 994 1365 2717 2825 3043 137
Size: 857 371 1352 108 218 3123
Fragments arranged by size:
3123 1352 857 371 218 108
SacII CC_GC'GG
Cuts at: 2984 2984
Size: 6029
SalI G'TCGA_C
Cuts at: 3066 3066
Size: 6029
SapI GCTCTTCn'nnn_
Cuts at: 4052 4262 4052
Size: 210 5819
SbfI CC_TGCA'GG
Cuts at: 3076 3076
Size: 6029
ScaI AGT'ACT
Cuts at: 999 2341 2419 5546 999
Size: 1342 78 3127 1482
Fragments arranged by size:
3127 1482 1342 78
SfcI C'TryA_G
Cuts at: 33 484 496 613 2505 2911 3072 3757 4750
Size: 451 12 117 1892 406 161 685 993
Cuts at: 4750 4941 33
Size: 191 1121
Fragments arranged by size:
1892 1121 993 685 451 406 191 161
117 12
SfoI GGC'GCC
Cuts at: 1735 3461 3709 1735
Size: 1726 248 4055
Fragments arranged by size:
4055 1726 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6029
SmaI CCC'GGG
Cuts at: 3051 3051
Size: 6029
SmlI C'TyrA_G
Cuts at: 881 1920 3184 4099 4591 4853 881
Size: 1039 1264 915 492 262 2057
Fragments arranged by size:
2057 1264 1039 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6029
SphI G_CATG'C
Cuts at: 2754 3082 3582 2754
Size: 328 500 5201
Fragments arranged by size:
5201 500 328
StyI C'CwwG_G
Cuts at: 1997 2438 2594 3163 3517 1997
Size: 441 156 569 354 4509
Fragments arranged by size:
4509 569 441 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 3612 3745 4204 4413 1455
Size: 2157 133 459 209 3071
Fragments arranged by size:
3071 2157 459 209 133
TatI w'GTAC_w
Cuts at: 997 1451 2339 2417 2654 5544 997
Size: 454 888 78 237 2890 1482
Fragments arranged by size:
2890 1482 888 454 237 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1312 1344 2038 2070 5546 5578 1312
Size: 32 694 32 3476 32 1763
Fragments arranged by size:
3476 1763 694 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2620 3826 2620
Size: 1206 4823
XbaI T'CTAG_A
Cuts at: 3060 3060
Size: 6029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1092 1228 2601 2943 3524 1092
Size: 136 1373 342 581 3597
Fragments arranged by size:
3597 1373 581 342 136
XmaI C'CCGG_G
Cuts at: 3049 3049
Size: 6029
XmnI GAAnn'nnTTC
Cuts at: 2572 2572
Size: 6029
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BmtI BsiWI BssHII BstXI BstZ17I CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PasI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI