Restriction map for
pF1KE2585
(Circular) (Six-base) MAPSORT of: pF1KE2585.seq Check: 4046 from: 1 to: 5627
pF1KE2585 5627 bp
With 182 enzymes: SgfI *
September 4, 2015 15:51 ..
AccI GT'mk_AC
Cuts at: 1309 2665 1309
Size: 1356 4271
Acc65I G'GTAC_C
Cuts at: 2643 2643
Size: 5627
AclI AA'CG_TT
Cuts at: 5467 5467
Size: 5627
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 481 1619 3452 3884 4631 4899 451
Size: 30 1138 1833 432 747 268 1179
Fragments arranged by size:
1833 1179 1138 747 432 268 30
AflIII A'CryG_T
Cuts at: 1970 2979 4083 5060 1970
Size: 1009 1104 977 2537
Fragments arranged by size:
2537 1104 1009 977
AgeI A'CCGG_T
Cuts at: 352 2563 4015 352
Size: 2211 1452 1964
Fragments arranged by size:
2211 1964 1452
AleI CACnn'nnGTG
Cuts at: 2061 2061
Size: 5627
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1001 1001
Size: 5627
AlwNI CAG_nnn'CTG
Cuts at: 169 1996 2932 4499 169
Size: 1827 936 1567 1297
Fragments arranged by size:
1827 1567 1297 936
ApaI G_GGCC'C
Cuts at: 2340 2340
Size: 5627
ApaLI G'TGCA_C
Cuts at: 772 4397 772
Size: 3625 2002
ApoI r'AATT_y
Cuts at: 256 493 2030 2548 2631 4980 5236 256
Size: 237 1537 518 83 2349 256 647
Fragments arranged by size:
2349 1537 647 518 256 237 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 5627
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
AvaI C'yCGr_G
Cuts at: 380 2647 380
Size: 2267 3360
BamHI G'GATC_C
Cuts at: 558 750 1415 2652 558
Size: 192 665 1237 3533
Fragments arranged by size:
3533 1237 665 192
BanI G'GyrC_C
Cuts at: 1100 2643 3057 3305 3340 1100
Size: 1543 414 248 35 3387
Fragments arranged by size:
3387 1543 414 248 35
BanII G_rGCy'C
Cuts at: 2340 2641 3671 2340
Size: 301 1030 4296
Fragments arranged by size:
4296 1030 301
BbeI G_GCGC'C
Cuts at: 3061 3309 3061
Size: 248 5379
BbvCI CC'TCA_GC
Cuts at: 2341 2341
Size: 5627
BciVI GTATCCnnnnn_n'
Cuts at: 3525 4286 5627 3525
Size: 761 1341 3525
Fragments arranged by size:
3525 1341 761
BclI T'GATC_A
Cuts at: 2483 3147 2483
Size: 664 4963
BglII A'GATC_T
Cuts at: 3142 5179 3142
Size: 2037 3590
BlpI GC'TnA_GC
Cuts at: 2739 2739
Size: 5627
Bme1580I G_kGCm'C
Cuts at: 776 1105 1150 2340 3252 3345 4401 776
Size: 329 45 1190 912 93 1056 2002
Fragments arranged by size:
2002 1190 1056 912 329 93 45
BmrI ACTGGGnnnn_n'
Cuts at: 2538 2839 2920 3255 5399 2538
Size: 301 81 335 2144 2766
Fragments arranged by size:
2766 2144 335 301 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 874 2514 3994 874
Size: 1640 1480 2507
Fragments arranged by size:
2507 1640 1480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 926 1584 1670 766
Size: 160 658 86 4723
Fragments arranged by size:
4723 658 160 86
Bpu10I CC'TnA_GC
Cuts at: 2341 2401 2341
Size: 60 5567
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2803 3682 4174 4472 2803
Size: 879 492 298 3958
Fragments arranged by size:
3958 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5331 5331
Size: 5627
BsaAI yAC'GTr
Cuts at: 771 2980 771
Size: 2209 3418
BsaBI GATnn'nnATC
Cuts at: 6 2392 3141 5178 6
Size: 2386 749 2037 455
Fragments arranged by size:
2386 2037 749 455
BsaHI Gr'CG_yC
Cuts at: 3058 3306 4008 5504 3058
Size: 248 702 1496 3181
Fragments arranged by size:
3181 1496 702 248
BsaWI w'CCGG_w
Cuts at: 122 352 2563 2816 3337 4015 4289 4436 4847
Size: 230 2211 253 521 678 274 147 411
Cuts at: 4847 122
Size: 902
Fragments arranged by size:
2211 902 678 521 411 274 253 230
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1105 1135 2197 2227 2302 2332 2414 2444 1105
Size: 30 1062 30 75 30 82 30 4288
Fragments arranged by size:
4288 1062 82 75 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2133 2194 2133
Size: 61 5566
BseYI C'CCAG_C
Cuts at: 146 1638 3052 3810 4387 4883 146
Size: 1492 1414 758 577 496 890
Fragments arranged by size:
1492 1414 890 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1576 3169 3215 4423 104
Size: 1472 1593 46 1208 1308
Fragments arranged by size:
1593 1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 776 2641 3419 3609 4401 776
Size: 1865 778 190 792 2002
Fragments arranged by size:
2002 1865 792 778 190
BsmI GAATG_Cn'
Cuts at: 1944 1944
Size: 5627
BsmBI CGTCTCn'nnnn_
Cuts at: 1597 1597
Size: 5627
Bsp1286I G_dGCh'C
Cuts at: 776 1105 1150 2340 2641 3252 3345 3419 3609
Size: 329 45 1190 301 611 93 74 190
Cuts at: 3609 3671 4401 776
Size: 62 730 2002
Fragments arranged by size:
2002 1190 730 611 329 301 190 93
74 62 45
BspEI T'CCGG_A
Cuts at: 122 4847 122
Size: 4725 902
BspHI T'CATG_A
Cuts at: 1862 4803 1862
Size: 2941 2686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2677 2891 3193 3574 2677
Size: 214 302 381 4730
Fragments arranged by size:
4730 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3919 3973 5457 5622 50
Size: 3869 54 1484 165 55
Fragments arranged by size:
3869 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 3539 1697
Size: 1842 3785
BsrFI r'CCGG_y
Cuts at: 352 2563 3625 3806 4015 352
Size: 2211 1062 181 209 1964
Fragments arranged by size:
2211 1964 1062 209 181
BsrGI T'GTAC_A
Cuts at: 1502 1502
Size: 5627
BssSI C'ACGA_G
Cuts at: 3898 4256 3898
Size: 358 5269
BstAPI GCAn_nnn'nTGC
Cuts at: 3004 3004
Size: 5627
BstBI TT'CG_AA
Cuts at: 3988 3988
Size: 5627
BstYI r'GATC_y
Cuts at: 1 558 750 1415 2652 3119 3142 3477 3723
Size: 557 192 665 1237 467 23 335 246
Cuts at: 3723 4735 4821 4833 5179 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1237 1012 665 557 467 449 346 335
246 192 86 23 12
BstZ17I GTA'TAC
Cuts at: 1310 1310
Size: 5627
BtgI C'CryG_G
Cuts at: 107 2258 2381 2468 107
Size: 2151 123 87 3266
Fragments arranged by size:
3266 2151 123 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3577 3758 3921 3577
Size: 181 163 5283
Fragments arranged by size:
5283 181 163
BtsI GCAGTG_nn'
Cuts at: 2312 2447 2515 2870 2312
Size: 135 68 355 5069
Fragments arranged by size:
5069 355 135 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2130 2165 2130
Size: 35 5592
DraI TTT'AAA
Cuts at: 2626 2626
Size: 5627
DrdI GACnn_nn'nnGTC
Cuts at: 2260 3333 4191 2260
Size: 1073 858 3696
Fragments arranged by size:
3696 1073 858
EaeI y'GGCC_r
Cuts at: 1573 2471 3212 3386 3777 3804 5149 1573
Size: 898 741 174 391 27 1345 2051
Fragments arranged by size:
2051 1345 898 741 391 174 27
EagI C'GGCC_G
Cuts at: 1573 3212 1573
Size: 1639 3988
EarI CTCTTCn'nnn_
Cuts at: 457 569 606 1595 2404 2612 3650 3860 457
Size: 112 37 989 809 208 1038 210 2224
Fragments arranged by size:
2224 1038 989 809 210 208 112 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 571 3835 4145 4291 5437 571
Size: 3264 310 146 1146 761
Fragments arranged by size:
3264 1146 761 310 146
EcoICRI GAG'CTC
Cuts at: 2639 2639
Size: 5627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 481 766 926 1584 1619 1670 3452 3884
Size: 30 285 160 658 35 51 1782 432
Cuts at: 3884 4631 4899 451
Size: 747 268 1179
Fragments arranged by size:
1782 1179 747 658 432 285 268 160
51 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 721 721
Size: 5627
EcoO109I rG'GnC_Cy
Cuts at: 2337 2493 2766 2337
Size: 156 273 5198
Fragments arranged by size:
5198 273 156
EcoRI G'AATT_C
Cuts at: 2030 2631 2030
Size: 601 5026
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 486 1624 3646 486
Size: 1138 2022 2467
Fragments arranged by size:
2467 2022 1138
FspI TGC'GCA
Cuts at: 2357 3408 2357
Size: 1051 4576
HaeII r_GCGC'y
Cuts at: 3061 3309 4331 5310 3061
Size: 248 1022 979 3378
Fragments arranged by size:
3378 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 543 568 575 600 912 944 1105 1137 2197
Size: 25 7 25 312 32 161 32 1060
Cuts at: 2197 2229 3469 3499 3501 3531 543
Size: 32 1240 30 2 30 2639
Fragments arranged by size:
2639 1240 1060 312 161 32 32 32
30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 2666 2666
Size: 5627
HindIII A'AGCT_T
Cuts at: 298 298
Size: 5627
KasI G'GCGC_C
Cuts at: 3057 3305 3057
Size: 248 5379
KpnI G_GTAC'C
Cuts at: 2647 2647
Size: 5627
MluI A'CGCG_T
Cuts at: 5060 5060
Size: 5627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 273 551 2333 4298 4482 273
Size: 278 1782 1965 184 1418
Fragments arranged by size:
1965 1782 1418 278 184
MscI TGG'CCA
Cuts at: 2473 3388 2473
Size: 915 4712
MslI CAynn'nnrTG
Cuts at: 1015 1066 1372 1768 1969 2061 3616 3743 1015
Size: 51 306 396 201 92 1555 127 2899
Fragments arranged by size:
2899 1555 396 306 201 127 92 51
MspA1I CmG'CkG
Cuts at: 813 985 1632 2361 2558 2738 3052 3412 4425
Size: 172 647 729 197 180 314 360 1013
Cuts at: 4425 4670 813
Size: 245 1770
Fragments arranged by size:
1770 1013 729 647 360 314 245 197
180 172
NaeI GCC'GGC
Cuts at: 3808 3808
Size: 5627
NarI GG'CG_CC
Cuts at: 3058 3306 3058
Size: 248 5379
NcoI C'CATG_G
Cuts at: 107 2258 2381 107
Size: 2151 123 3353
Fragments arranged by size:
3353 2151 123
NdeI CA'TA_TG
Cuts at: 1769 1769
Size: 5627
NgoMIV G'CCGG_C
Cuts at: 3806 3806
Size: 5627
NspI r_CATG'y
Cuts at: 687 1974 2680 3180 4087 687
Size: 1287 706 500 907 2227
Fragments arranged by size:
2227 1287 907 706 500
PciI A'CATG_T
Cuts at: 1970 4083 1970
Size: 2113 3514
PflMI CCAn_nnn'nTGG
Cuts at: 1996 1996
Size: 5627
PfoI T'CCnGG_A
Cuts at: 473 1561 473
Size: 1088 4539
PmeI GTTT'AAAC
Cuts at: 2626 2626
Size: 5627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 4766
Size: 32 5595
PshAI GACnn'nnGTC
Cuts at: 1358 1358
Size: 5627
PsiI TTA'TAA
Cuts at: 1589 1589
Size: 5627
PspOMI G'GGCC_C
Cuts at: 2336 2336
Size: 5627
PstI C_TGCA'G
Cuts at: 980 2674 3359 980
Size: 1694 685 3248
Fragments arranged by size:
3248 1694 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5627
PvuII CAG'CTG
Cuts at: 813 985 1632 2361 3052 3412 813
Size: 172 647 729 691 360 3028
Fragments arranged by size:
3028 729 691 647 360 172
RsrII CG'GwC_CG
Cuts at: 2170 3822 2170
Size: 1652 3975
SacI G_AGCT'C
Cuts at: 2641 2641
Size: 5627
SalI G'TCGA_C
Cuts at: 2664 2664
Size: 5627
SapI GCTCTTCn'nnn_
Cuts at: 2404 3650 3860 2404
Size: 1246 210 4171
Fragments arranged by size:
4171 1246 210
SbfI CC_TGCA'GG
Cuts at: 2674 2674
Size: 5627
ScaI AGT'ACT
Cuts at: 2290 5144 2290
Size: 2854 2773
SfcI C'TryA_G
Cuts at: 33 157 976 1627 2553 2670 3355 4348 4539
Size: 124 819 651 926 117 685 993 191
Cuts at: 4539 33
Size: 1121
Fragments arranged by size:
1121 993 926 819 685 651 191 124
117
SfoI GGC'GCC
Cuts at: 3059 3307 3059
Size: 248 5379
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
SgrAI Cr'CCGG_yG
Cuts at: 352 352
Size: 5627
SmaI CCC'GGG
Cuts at: 382 2649 382
Size: 2267 3360
SmlI C'TyrA_G
Cuts at: 2782 3697 4189 4451 2782
Size: 915 492 262 3958
Fragments arranged by size:
3958 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5627
SphI G_CATG'C
Cuts at: 687 2680 3180 687
Size: 1993 500 3134
Fragments arranged by size:
3134 1993 500
StyI C'CwwG_G
Cuts at: 107 801 1755 2071 2258 2381 2761 3115 107
Size: 694 954 316 187 123 380 354 2619
Fragments arranged by size:
2619 954 694 380 354 316 187 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 499 1348 1477 2187 2233 3210 3343 3802 4011
Size: 849 129 710 46 977 133 459 209
Cuts at: 4011 499
Size: 2115
Fragments arranged by size:
2115 977 849 710 459 209 133 129
46
TatI w'GTAC_w
Cuts at: 1502 2288 5142 1502
Size: 786 2854 1987
Fragments arranged by size:
2854 1987 786
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1147 1179 2414 2446 5144 5176 1147
Size: 32 1235 32 2698 32 1598
Fragments arranged by size:
2698 1598 1235 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3424 3424
Size: 5627
XbaI T'CTAG_A
Cuts at: 2658 2658
Size: 5627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1774 2466 3122 1774
Size: 692 656 4279
Fragments arranged by size:
4279 692 656
XmaI C'CCGG_G
Cuts at: 380 2647 380
Size: 2267 3360
XmnI GAAnn'nnTTC
Cuts at: 244 2548 244
Size: 2304 3323
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BssHII BstEII BstXI Bsu36I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
ZraI