Restriction map for
pF1KE2580
(Circular) (Six-base) MAPSORT of: pF1KE2580.seq Check: 9550 from: 1 to: 6695
pF1KE2580 6695 bp
With 182 enzymes: SgfI *
July 27, 2015 09:05 ..
AccI GT'mk_AC
Cuts at: 824 3025 3733 824
Size: 2201 708 3786
Fragments arranged by size:
3786 2201 708
Acc65I G'GTAC_C
Cuts at: 2123 3711 2123
Size: 1588 5107
AclI AA'CG_TT
Cuts at: 3689 6535 3689
Size: 2846 3849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 1529 4520 4952 5699 5967 498
Size: 1031 2991 432 747 268 1226
Fragments arranged by size:
2991 1226 1031 747 432 268
AflIII A'CryG_T
Cuts at: 1880 4047 5151 6128 1880
Size: 2167 1104 977 2447
Fragments arranged by size:
2447 2167 1104 977
AgeI A'CCGG_T
Cuts at: 5083 5083
Size: 6695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1465 2229 3349 1465
Size: 764 1120 4811
Fragments arranged by size:
4811 1120 764
AlwNI CAG_nnn'CTG
Cuts at: 3120 3300 4000 5567 3120
Size: 180 700 1567 4248
Fragments arranged by size:
4248 1567 700 180
ApaLI G'TGCA_C
Cuts at: 5465 5465
Size: 6695
ApoI r'AATT_y
Cuts at: 124 596 878 1645 1651 1936 2098 2317 2626
Size: 472 282 767 6 285 162 219 309
Cuts at: 2626 2925 3110 3541 3699 6048 6304 124
Size: 299 185 431 158 2349 256 515
Fragments arranged by size:
2349 767 515 472 431 309 299 285
282 256 219 185 162 158 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 6695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
AvaI C'yCGr_G
Cuts at: 3715 3715
Size: 6695
BamHI G'GATC_C
Cuts at: 333 2462 3720 333
Size: 2129 1258 3308
Fragments arranged by size:
3308 2129 1258
BanI G'GyrC_C
Cuts at: 2123 2263 3711 4125 4373 4408 2123
Size: 140 1448 414 248 35 4410
Fragments arranged by size:
4410 1448 414 248 140 35
BanII G_rGCy'C
Cuts at: 710 2786 3709 4739 710
Size: 2076 923 1030 2666
Fragments arranged by size:
2666 2076 1030 923
BbeI G_GCGC'C
Cuts at: 4129 4377 4129
Size: 248 6447
BbsI GAAGACnn'nnnn_
Cuts at: 670 1011 1121 1214 1877 1927 2873 670
Size: 341 110 93 663 50 946 4492
Fragments arranged by size:
4492 946 663 341 110 93 50
BbvCI CC'TCA_GC
Cuts at: 3206 3235 3206
Size: 29 6666
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1098 1132 1948 1982 3595 3629 1098
Size: 34 816 34 1613 34 4164
Fragments arranged by size:
4164 1613 816 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4593 5354 6695 4593
Size: 761 1341 4593
Fragments arranged by size:
4593 1341 761
BclI T'GATC_A
Cuts at: 1811 3188 4215 1811
Size: 1377 1027 4291
Fragments arranged by size:
4291 1377 1027
BglI GCCn_nnn'nGGC
Cuts at: 907 907
Size: 6695
BglII A'GATC_T
Cuts at: 420 777 1383 4210 6247 420
Size: 357 606 2827 2037 868
Fragments arranged by size:
2827 2037 868 606 357
BlpI GC'TnA_GC
Cuts at: 3807 3807
Size: 6695
Bme1580I G_kGCm'C
Cuts at: 2705 4320 4413 5469 2705
Size: 1615 93 1056 3931
Fragments arranged by size:
3931 1615 1056 93
BmgBI CAC'GTC
Cuts at: 2700 2700
Size: 6695
BmrI ACTGGGnnnn_n'
Cuts at: 677 1009 2699 3907 3988 4323 6467 677
Size: 332 1690 1208 81 335 2144 905
Fragments arranged by size:
2144 1690 1208 905 335 332 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5062 5062
Size: 6695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 1577
Size: 6695
Bpu10I CC'TnA_GC
Cuts at: 3206 3235 3206
Size: 29 6666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 649 2436 2837 3871 4750 5242 5540 532
Size: 117 1787 401 1034 879 492 298 1687
Fragments arranged by size:
1787 1687 1034 879 492 401 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 3218 6399 3218
Size: 3181 3514
BsaAI yAC'GTr
Cuts at: 2271 2311 4048 2271
Size: 40 1737 4918
Fragments arranged by size:
4918 1737 40
BsaBI GATnn'nnATC
Cuts at: 6 4209 6246 6
Size: 4203 2037 455
Fragments arranged by size:
4203 2037 455
BsaHI Gr'CG_yC
Cuts at: 4126 4374 5076 6572 4126
Size: 248 702 1496 4249
Fragments arranged by size:
4249 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3884 4405 5083 5357 5504 5915 3884
Size: 521 678 274 147 411 4664
Fragments arranged by size:
4664 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1139 1169 1976 2006 2585 2615 1139
Size: 30 807 30 579 30 5219
Fragments arranged by size:
5219 807 579 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 1618 1642 3223 202
Size: 1416 24 1581 3674
Fragments arranged by size:
3674 1581 1416 24
BseYI C'CCAG_C
Cuts at: 1190 1572 1823 3293 4120 4878 5455 5951 1190
Size: 382 251 1470 827 758 577 496 1934
Fragments arranged by size:
1934 1470 827 758 577 496 382 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2242 2964 3214 2242
Size: 722 250 5723
Fragments arranged by size:
5723 722 250
BsiEI CG_ry'CG
Cuts at: 104 316 4237 4283 5491 104
Size: 212 3921 46 1208 1308
Fragments arranged by size:
3921 1308 1208 212 46
BsiHKAI G_wGCw'C
Cuts at: 2786 3342 3709 4487 4677 5469 2786
Size: 556 367 778 190 792 4012
Fragments arranged by size:
4012 792 778 556 367 190
BsmI GAATG_Cn'
Cuts at: 295 295
Size: 6695
Bsp1286I G_dGCh'C
Cuts at: 710 2705 2786 3342 3709 4320 4413 4487 4677
Size: 1995 81 556 367 611 93 74 190
Cuts at: 4677 4739 5469 710
Size: 62 730 1936
Fragments arranged by size:
1995 1936 730 611 556 367 190 93
81 74 62
BspEI T'CCGG_A
Cuts at: 5915 5915
Size: 6695
BspHI T'CATG_A
Cuts at: 2144 3030 5871 2144
Size: 886 2841 2968
Fragments arranged by size:
2968 2841 886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2214 3236 3745 3959 4261 4642 2214
Size: 1022 509 214 302 381 4267
Fragments arranged by size:
4267 1022 509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4987 5041 6525 6690 50
Size: 4937 54 1484 165 55
Fragments arranged by size:
4937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 484 917 1247 3059 4607 484
Size: 433 330 1812 1548 2572
Fragments arranged by size:
2572 1812 1548 433 330
BsrFI r'CCGG_y
Cuts at: 4693 4874 5083 4693
Size: 181 209 6305
Fragments arranged by size:
6305 209 181
BsrGI T'GTAC_A
Cuts at: 1142 1246 1142
Size: 104 6591
BssSI C'ACGA_G
Cuts at: 3280 4966 5324 3280
Size: 1686 358 4651
Fragments arranged by size:
4651 1686 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4072 4072
Size: 6695
BstBI TT'CG_AA
Cuts at: 2511 5056 2511
Size: 2545 4150
BstXI CCAn_nnnn'nTGG
Cuts at: 345 3504 345
Size: 3159 3536
BstYI r'GATC_y
Cuts at: 1 333 420 777 861 1383 1395 2462 2616
Size: 332 87 357 84 522 12 1067 154
Cuts at: 2616 3720 4187 4210 4545 4791 5803 5889 5901
Size: 1104 467 23 335 246 1012 86 12
Cuts at: 5901 6247 1
Size: 346 449
Fragments arranged by size:
1104 1067 1012 522 467 449 357 346
335 332 246 154 87 86 84 23
12 12
BtgI C'CryG_G
Cuts at: 107 2798 107
Size: 2691 4004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2092 4645 4826 4989 2092
Size: 2553 181 163 3798
Fragments arranged by size:
3798 2553 181 163
BtsI GCAGTG_nn'
Cuts at: 417 1338 2976 3250 3593 3938 417
Size: 921 1638 274 343 345 3174
Fragments arranged by size:
3174 1638 921 345 343 274
DraI TTT'AAA
Cuts at: 2903 2933 3694 2903
Size: 30 761 5904
Fragments arranged by size:
5904 761 30
DrdI GACnn_nn'nnGTC
Cuts at: 2410 4401 5259 2410
Size: 1991 858 3846
Fragments arranged by size:
3846 1991 858
EaeI y'GGCC_r
Cuts at: 110 2454 4280 4454 4845 4872 6217 110
Size: 2344 1826 174 391 27 1345 588
Fragments arranged by size:
2344 1826 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 4280 4280
Size: 6695
EarI CTCTTCn'nnn_
Cuts at: 226 1595 2363 4718 4928 226
Size: 1369 768 2355 210 1993
Fragments arranged by size:
2355 1993 1369 768 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 408 4903 5213 5359 6505 408
Size: 4495 310 146 1146 598
Fragments arranged by size:
4495 1146 598 310 146
EcoICRI GAG'CTC
Cuts at: 2784 3707 2784
Size: 923 5772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 1529 1577 4520 4952 5699 5967 498
Size: 1031 48 2943 432 747 268 1226
Fragments arranged by size:
2943 1226 1031 747 432 268 48
EcoNI CCTnn'n_nnAGG
Cuts at: 1261 3046 1261
Size: 1785 4910
EcoO109I rG'GnC_Cy
Cuts at: 1719 3834 1719
Size: 2115 4580
EcoRI G'AATT_C
Cuts at: 1645 2317 3541 3699 1645
Size: 672 1224 158 4641
Fragments arranged by size:
4641 1224 672 158
EcoRV GAT'ATC
Cuts at: 991 1254 2494 2598 991
Size: 263 1240 104 5088
Fragments arranged by size:
5088 1240 263 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 669 2907 3544 4714 669
Size: 2238 637 1170 2650
Fragments arranged by size:
2650 2238 1170 637
FspI TGC'GCA
Cuts at: 4476 4476
Size: 6695
HaeII r_GCGC'y
Cuts at: 4129 4377 5399 6378 4129
Size: 248 1022 979 4446
Fragments arranged by size:
4446 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 744 776 1139 1171 1976 2008 2161
Size: 32 534 32 363 32 805 32 153
Cuts at: 2161 2193 2596 2628 3114 3121 3146 3153 4537
Size: 32 403 32 486 7 25 7 1384
Cuts at: 4537 4567 4569 4599 178
Size: 30 2 30 2274
Fragments arranged by size:
2274 1384 805 534 486 403 363 153
32 32 32 32 32 32 30 30
25 7 7 2
HincII GTy'rAC
Cuts at: 2991 3026 3734 2991
Size: 35 708 5952
Fragments arranged by size:
5952 708 35
HindIII A'AGCT_T
Cuts at: 1532 1532
Size: 6695
KasI G'GCGC_C
Cuts at: 4125 4373 4125
Size: 248 6447
KpnI G_GTAC'C
Cuts at: 2127 3715 2127
Size: 1588 5107
MfeI C'AATT_G
Cuts at: 1931 2083 1931
Size: 152 6543
MluI A'CGCG_T
Cuts at: 6128 6128
Size: 6695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 5366 5550 345
Size: 5021 184 1490
Fragments arranged by size:
5021 1490 184
MscI TGG'CCA
Cuts at: 112 2456 4456 112
Size: 2344 2000 2351
Fragments arranged by size:
2351 2344 2000
MslI CAynn'nnrTG
Cuts at: 925 2335 4684 4811 925
Size: 1410 2349 127 2809
Fragments arranged by size:
2809 2349 1410 127
MspA1I CmG'CkG
Cuts at: 1527 3300 3806 4120 4480 5493 5738 1527
Size: 1773 506 314 360 1013 245 2484
Fragments arranged by size:
2484 1773 1013 506 360 314 245
NaeI GCC'GGC
Cuts at: 4876 4876
Size: 6695
NarI GG'CG_CC
Cuts at: 4126 4374 4126
Size: 248 6447
NcoI C'CATG_G
Cuts at: 107 107
Size: 6695
NdeI CA'TA_TG
Cuts at: 2574 2574
Size: 6695
NgoMIV G'CCGG_C
Cuts at: 4874 4874
Size: 6695
NspI r_CATG'y
Cuts at: 545 1884 1930 2013 2947 3748 4248 5155 545
Size: 1339 46 83 934 801 500 907 2085
Fragments arranged by size:
2085 1339 934 907 801 500 83 46
PciI A'CATG_T
Cuts at: 1880 5151 1880
Size: 3271 3424
PflMI CCAn_nnn'nTGG
Cuts at: 1050 1062 1050
Size: 12 6683
PmeI GTTT'AAAC
Cuts at: 3694 3694
Size: 6695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5834 5866 5834
Size: 32 6663
PshAI GACnn'nnGTC
Cuts at: 1682 1682
Size: 6695
PsiI TTA'TAA
Cuts at: 1763 1877 1949 1763
Size: 114 72 6509
Fragments arranged by size:
6509 114 72
PstI C_TGCA'G
Cuts at: 412 1345 3742 4427 412
Size: 933 2397 685 2680
Fragments arranged by size:
2680 2397 933 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6695
PvuII CAG'CTG
Cuts at: 1527 3300 4120 4480 1527
Size: 1773 820 360 3742
Fragments arranged by size:
3742 1773 820 360
RsrII CG'GwC_CG
Cuts at: 4890 4890
Size: 6695
SacI G_AGCT'C
Cuts at: 2786 3709 2786
Size: 923 5772
SalI G'TCGA_C
Cuts at: 3024 3732 3024
Size: 708 5987
SapI GCTCTTCn'nnn_
Cuts at: 4718 4928 4718
Size: 210 6485
SbfI CC_TGCA'GG
Cuts at: 3742 3742
Size: 6695
ScaI AGT'ACT
Cuts at: 6212 6212
Size: 6695
SfcI C'TryA_G
Cuts at: 33 408 1341 1730 3738 4423 5416 5607 33
Size: 375 933 389 2008 685 993 191 1121
Fragments arranged by size:
2008 1121 993 933 685 389 375 191
SfoI GGC'GCC
Cuts at: 4127 4375 4127
Size: 248 6447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
SmaI CCC'GGG
Cuts at: 3717 3717
Size: 6695
SmlI C'TyrA_G
Cuts at: 547 664 2415 2852 3850 4765 5257 5519 547
Size: 117 1751 437 998 915 492 262 1723
Fragments arranged by size:
1751 1723 998 915 492 437 262 117
SnaBI TAC'GTA
Cuts at: 2271 2311 2271
Size: 40 6655
SpeI A'CTAG_T
Cuts at: 67 2683 67
Size: 2616 4079
SphI G_CATG'C
Cuts at: 3748 4248 3748
Size: 500 6195
SspI AAT'ATT
Cuts at: 1375 1869 2559 1375
Size: 494 690 5511
Fragments arranged by size:
5511 690 494
StuI AGG'CCT
Cuts at: 491 962 491
Size: 471 6224
StyI C'CwwG_G
Cuts at: 107 1979 3829 4183 107
Size: 1872 1850 354 2619
Fragments arranged by size:
2619 1872 1850 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 302 1894 4278 4411 4870 5079 302
Size: 1592 2384 133 459 209 1918
Fragments arranged by size:
2384 1918 1592 459 209 133
TatI w'GTAC_w
Cuts at: 1142 1246 1358 6210 1142
Size: 104 112 4852 1627
Fragments arranged by size:
4852 1627 112 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 387 419 2585 2617 6212 6244 387
Size: 32 2166 32 3595 32 838
Fragments arranged by size:
3595 2166 838 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4492 4492
Size: 6695
XbaI T'CTAG_A
Cuts at: 3537 3726 3537
Size: 189 6506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2805 4190 2805
Size: 1385 5310
XmaI C'CCGG_G
Cuts at: 3715 3715
Size: 6695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BfrBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HpaI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI XmnI ZraI