Restriction map for
pF1KE2576
(Circular) (Six-base) MAPSORT of: pF1KE2576.seq Check: 447 from: 1 to: 3620
pF1KE2576 3620 bp
With 182 enzymes: SgfI *
May 26, 2015 10:14 ..
AccI GT'mk_AC
Cuts at: 560 658 560
Size: 98 3522
Acc65I G'GTAC_C
Cuts at: 636 636
Size: 3620
AclI AA'CG_TT
Cuts at: 3460 3460
Size: 3620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1877 2624 2892 1445
Size: 432 747 268 2173
Fragments arranged by size:
2173 747 432 268
AflIII A'CryG_T
Cuts at: 521 972 2076 3053 521
Size: 451 1104 977 1088
Fragments arranged by size:
1104 1088 977 451
AgeI A'CCGG_T
Cuts at: 2008 2008
Size: 3620
AlwNI CAG_nnn'CTG
Cuts at: 925 2492 925
Size: 1567 2053
ApaLI G'TGCA_C
Cuts at: 2390 2390
Size: 3620
ApoI r'AATT_y
Cuts at: 624 2973 3229 624
Size: 2349 256 1015
Fragments arranged by size:
2349 1015 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3620
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
AvaI C'yCGr_G
Cuts at: 640 640
Size: 3620
BamHI G'GATC_C
Cuts at: 645 645
Size: 3620
BanI G'GyrC_C
Cuts at: 111 385 445 636 1050 1298 1333 111
Size: 274 60 191 414 248 35 2398
Fragments arranged by size:
2398 414 274 248 191 60 35
BanII G_rGCy'C
Cuts at: 634 1664 634
Size: 1030 2590
BbeI G_GCGC'C
Cuts at: 1054 1302 1054
Size: 248 3372
BbsI GAAGACnn'nnnn_
Cuts at: 153 345 153
Size: 192 3428
BciVI GTATCCnnnnn_n'
Cuts at: 1518 2279 3620 1518
Size: 761 1341 1518
Fragments arranged by size:
1518 1341 761
BclI T'GATC_A
Cuts at: 404 1140 404
Size: 736 2884
BglII A'GATC_T
Cuts at: 1135 3172 1135
Size: 2037 1583
BlpI GC'TnA_GC
Cuts at: 732 732
Size: 3620
Bme1580I G_kGCm'C
Cuts at: 448 1245 1338 2394 448
Size: 797 93 1056 1674
Fragments arranged by size:
1674 1056 797 93
BmgBI CAC'GTC
Cuts at: 546 546
Size: 3620
BmrI ACTGGGnnnn_n'
Cuts at: 832 913 1248 3392 832
Size: 81 335 2144 1060
Fragments arranged by size:
2144 1060 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 487 138
Size: 349 3271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 796 1675 2167 2465 364
Size: 432 879 492 298 1519
Fragments arranged by size:
1519 879 492 432 298
BsaI GGTCTCn'nnnn_
Cuts at: 3324 3324
Size: 3620
BsaAI yAC'GTr
Cuts at: 973 973
Size: 3620
BsaBI GATnn'nnATC
Cuts at: 6 1134 3171 6
Size: 1128 2037 455
Fragments arranged by size:
2037 1128 455
BsaHI Gr'CG_yC
Cuts at: 599 1051 1299 2001 3497 599
Size: 452 248 702 1496 722
Fragments arranged by size:
1496 722 702 452 248
BsaWI w'CCGG_w
Cuts at: 409 809 1330 2008 2282 2429 2840 409
Size: 400 521 678 274 147 411 1189
Fragments arranged by size:
1189 678 521 411 400 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 484 514 484
Size: 30 3590
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 484 523 316
Size: 168 39 3413
Fragments arranged by size:
3413 168 39
BseYI C'CCAG_C
Cuts at: 1045 1803 2380 2876 1045
Size: 758 577 496 1789
Fragments arranged by size:
1789 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1162 1208 2416 104
Size: 1058 46 1208 1308
Fragments arranged by size:
1308 1208 1058 46
BsiHKAI G_wGCw'C
Cuts at: 634 1412 1602 2394 634
Size: 778 190 792 1860
Fragments arranged by size:
1860 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 448 634 1245 1338 1412 1602 1664 2394 448
Size: 186 611 93 74 190 62 730 1674
Fragments arranged by size:
1674 730 611 190 186 93 74 62
BspEI T'CCGG_A
Cuts at: 2840 2840
Size: 3620
BspHI T'CATG_A
Cuts at: 2796 2796
Size: 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 670 884 1186 1567 670
Size: 214 302 381 2723
Fragments arranged by size:
2723 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1912 1966 3450 3615 50
Size: 1862 54 1484 165 55
Fragments arranged by size:
1862 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1532 1532
Size: 3620
BsrFI r'CCGG_y
Cuts at: 1618 1799 2008 1618
Size: 181 209 3230
Fragments arranged by size:
3230 209 181
BssSI C'ACGA_G
Cuts at: 1891 2249 1891
Size: 358 3262
BstAPI GCAn_nnn'nTGC
Cuts at: 997 997
Size: 3620
BstBI TT'CG_AA
Cuts at: 1981 1981
Size: 3620
BstYI r'GATC_y
Cuts at: 1 185 645 1112 1135 1470 1716 2728 2814
Size: 184 460 467 23 335 246 1012 86
Cuts at: 2814 2826 3172 1
Size: 12 346 449
Fragments arranged by size:
1012 467 460 449 346 335 246 184
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 395 434 395
Size: 39 3581
BtgI C'CryG_G
Cuts at: 107 275 107
Size: 168 3452
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1570 1751 1914 1570
Size: 181 163 3276
Fragments arranged by size:
3276 181 163
BtsI GCAGTG_nn'
Cuts at: 493 863 493
Size: 370 3250
DraI TTT'AAA
Cuts at: 619 619
Size: 3620
DraIII CAC_nnn'GTG
Cuts at: 488 488
Size: 3620
DrdI GACnn_nn'nnGTC
Cuts at: 1326 2184 1326
Size: 858 2762
EaeI y'GGCC_r
Cuts at: 1205 1379 1770 1797 3142 1205
Size: 174 391 27 1345 1683
Fragments arranged by size:
1683 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1205 1205
Size: 3620
EarI CTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 161 1828 2138 2284 3430 161
Size: 1667 310 146 1146 351
Fragments arranged by size:
1667 1146 351 310 146
EcoICRI GAG'CTC
Cuts at: 632 632
Size: 3620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 487 1445 1877 2624 2892 138
Size: 349 958 432 747 268 866
Fragments arranged by size:
958 866 747 432 349 268
EcoO109I rG'GnC_Cy
Cuts at: 252 759 252
Size: 507 3113
EcoRI G'AATT_C
Cuts at: 624 624
Size: 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1639 1639
Size: 3620
FspI TGC'GCA
Cuts at: 1401 1401
Size: 3620
HaeII r_GCGC'y
Cuts at: 1054 1302 2324 3303 1054
Size: 248 1022 979 1371
Fragments arranged by size:
1371 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 1462 1492 1494 1524 484
Size: 32 946 30 2 30 2580
Fragments arranged by size:
2580 946 32 30 30 2
HincII GTy'rAC
Cuts at: 561 659 561
Size: 98 3522
KasI G'GCGC_C
Cuts at: 1050 1298 1050
Size: 248 3372
KpnI G_GTAC'C
Cuts at: 640 640
Size: 3620
MluI A'CGCG_T
Cuts at: 3053 3053
Size: 3620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 287 2291 2475 287
Size: 2004 184 1432
Fragments arranged by size:
2004 1432 184
MscI TGG'CCA
Cuts at: 1381 1381
Size: 3620
MslI CAynn'nnrTG
Cuts at: 1609 1736 1609
Size: 127 3493
MspA1I CmG'CkG
Cuts at: 332 731 1045 1405 2418 2663 332
Size: 399 314 360 1013 245 1289
Fragments arranged by size:
1289 1013 399 360 314 245
NaeI GCC'GGC
Cuts at: 1801 1801
Size: 3620
NarI GG'CG_CC
Cuts at: 1051 1299 1051
Size: 248 3372
NcoI C'CATG_G
Cuts at: 107 275 107
Size: 168 3452
NgoMIV G'CCGG_C
Cuts at: 1799 1799
Size: 3620
NspI r_CATG'y
Cuts at: 525 673 1173 2080 525
Size: 148 500 907 2065
Fragments arranged by size:
2065 907 500 148
PciI A'CATG_T
Cuts at: 521 2076 521
Size: 1555 2065
PfoI T'CCnGG_A
Cuts at: 188 464 188
Size: 276 3344
PmeI GTTT'AAAC
Cuts at: 619 619
Size: 3620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 3588
PpuMI rG'GwC_Cy
Cuts at: 252 252
Size: 3620
PstI C_TGCA'G
Cuts at: 667 1352 667
Size: 685 2935
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3620
PvuII CAG'CTG
Cuts at: 1045 1405 1045
Size: 360 3260
RsrII CG'GwC_CG
Cuts at: 1815 1815
Size: 3620
SacI G_AGCT'C
Cuts at: 634 634
Size: 3620
SalI G'TCGA_C
Cuts at: 559 657 559
Size: 98 3522
SapI GCTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
SbfI CC_TGCA'GG
Cuts at: 667 667
Size: 3620
ScaI AGT'ACT
Cuts at: 3137 3137
Size: 3620
SfcI C'TryA_G
Cuts at: 33 663 1348 2341 2532 33
Size: 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 191
SfoI GGC'GCC
Cuts at: 1052 1300 1052
Size: 248 3372
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
SmaI CCC'GGG
Cuts at: 642 642
Size: 3620
SmlI C'TyrA_G
Cuts at: 379 775 1690 2182 2444 379
Size: 396 915 492 262 1555
Fragments arranged by size:
1555 915 492 396 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3620
SphI G_CATG'C
Cuts at: 673 1173 673
Size: 500 3120
StuI AGG'CCT
Cuts at: 205 205
Size: 3620
StyI C'CwwG_G
Cuts at: 107 275 754 1108 107
Size: 168 479 354 2619
Fragments arranged by size:
2619 479 354 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 1203 1336 1795 2004 129
Size: 1074 133 459 209 1745
Fragments arranged by size:
1745 1074 459 209 133
TatI w'GTAC_w
Cuts at: 3135 3135
Size: 3620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3588
Tth111I GACn'n_nGTC
Cuts at: 1417 1417
Size: 3620
XbaI T'CTAG_A
Cuts at: 651 651
Size: 3620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 229 1115 229
Size: 886 2734
XmaI C'CCGG_G
Cuts at: 640 640
Size: 3620
XmnI GAAnn'nnTTC
Cuts at: 460 460
Size: 3620
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI