Restriction map for
pF1KE2570
(Circular) (Six-base) MAPSORT of: pF1KE2570.seq Check: 7688 from: 1 to: 4691
pF1KE2570 4691 bp
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AccI GT'mk_AC
Cuts at: 1729 1729
Size: 4691
Acc65I G'GTAC_C
Cuts at: 1707 1707
Size: 4691
AclI AA'CG_TT
Cuts at: 4531 4531
Size: 4691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 1368 2516 2948 3695 3963 1315
Size: 53 1148 432 747 268 2043
Fragments arranged by size:
2043 1148 747 432 268 53
AflIII A'CryG_T
Cuts at: 2043 3147 4124 2043
Size: 1104 977 2610
Fragments arranged by size:
2610 1104 977
AgeI A'CCGG_T
Cuts at: 3079 3079
Size: 4691
AhdI GACnn_n'nnGTC
Cuts at: 851 851
Size: 4691
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 4691
AlwNI CAG_nnn'CTG
Cuts at: 1996 3563 1996
Size: 1567 3124
ApaLI G'TGCA_C
Cuts at: 3461 3461
Size: 4691
ApoI r'AATT_y
Cuts at: 740 1695 4044 4300 740
Size: 955 2349 256 1131
Fragments arranged by size:
2349 1131 955 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4691
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
AvaI C'yCGr_G
Cuts at: 717 792 1711 717
Size: 75 919 3697
Fragments arranged by size:
3697 919 75
BamHI G'GATC_C
Cuts at: 1716 1716
Size: 4691
BanI G'GyrC_C
Cuts at: 1707 2121 2369 2404 1707
Size: 414 248 35 3994
Fragments arranged by size:
3994 414 248 35
BanII G_rGCy'C
Cuts at: 475 913 958 1705 2735 475
Size: 438 45 747 1030 2431
Fragments arranged by size:
2431 1030 747 438 45
BbeI G_GCGC'C
Cuts at: 2125 2373 2125
Size: 248 4443
BbsI GAAGACnn'nnnn_
Cuts at: 1679 1679
Size: 4691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4657
BciVI GTATCCnnnnn_n'
Cuts at: 372 2589 3350 4691 372
Size: 2217 761 1341 372
Fragments arranged by size:
2217 1341 761 372
BclI T'GATC_A
Cuts at: 2211 2211
Size: 4691
BglII A'GATC_T
Cuts at: 2206 4243 2206
Size: 2037 2654
BlpI GC'TnA_GC
Cuts at: 395 1803 395
Size: 1408 3283
Bme1580I G_kGCm'C
Cuts at: 2316 2409 3465 2316
Size: 93 1056 3542
Fragments arranged by size:
3542 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 406 1041 1903 1984 2319 4463 406
Size: 635 862 81 335 2144 634
Fragments arranged by size:
2144 862 635 634 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 4691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 834 942 236
Size: 598 108 3985
Fragments arranged by size:
3985 598 108
Bpu10I CC'TnA_GC
Cuts at: 444 444
Size: 4691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1867 2746 3238 3536 1072
Size: 795 879 492 298 2227
Fragments arranged by size:
2227 879 795 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 940 1555 4395 940
Size: 615 2840 1236
Fragments arranged by size:
2840 1236 615
BsaAI yAC'GTr
Cuts at: 2044 2044
Size: 4691
BsaBI GATnn'nnATC
Cuts at: 6 2205 4242 6
Size: 2199 2037 455
Fragments arranged by size:
2199 2037 455
BsaHI Gr'CG_yC
Cuts at: 2122 2370 3072 4568 2122
Size: 248 702 1496 2245
Fragments arranged by size:
2245 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1362 1880 2401 3079 3353 3500 3911 1362
Size: 518 521 678 274 147 411 2142
Fragments arranged by size:
2142 678 521 518 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 990 1020 990
Size: 30 4661
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 449 1381 1423 1519 1681 449
Size: 932 42 96 162 3459
Fragments arranged by size:
3459 932 162 96 42
BseYI C'CCAG_C
Cuts at: 290 1611 2116 2874 3451 3947 290
Size: 1321 505 758 577 496 1034
Fragments arranged by size:
1321 1034 758 577 505 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 919 1133 512
Size: 407 214 4070
Fragments arranged by size:
4070 407 214
BsiEI CG_ry'CG
Cuts at: 104 2233 2279 3487 104
Size: 2129 46 1208 1308
Fragments arranged by size:
2129 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 301 484 958 1705 2483 2673 3465 301
Size: 183 474 747 778 190 792 1527
Fragments arranged by size:
1527 792 778 747 474 190 183
BsmI GAATG_Cn'
Cuts at: 210 210
Size: 4691
Bsp1286I G_dGCh'C
Cuts at: 301 475 484 913 958 1705 2316 2409 2483
Size: 174 9 429 45 747 611 93 74
Cuts at: 2483 2673 2735 3465 301
Size: 190 62 730 1527
Fragments arranged by size:
1527 747 730 611 429 190 174 93
74 62 45 9
BspEI T'CCGG_A
Cuts at: 3911 3911
Size: 4691
BspHI T'CATG_A
Cuts at: 1020 3867 1020
Size: 2847 1844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1741 1955 2257 2638 1741
Size: 214 302 381 3794
Fragments arranged by size:
3794 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1290 2983 3037 4521 4686 50
Size: 1240 1693 54 1484 165 55
Fragments arranged by size:
1693 1484 1240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2603 2603
Size: 4691
BsrFI r'CCGG_y
Cuts at: 2689 2870 3079 2689
Size: 181 209 4301
Fragments arranged by size:
4301 209 181
BssHII G'CGCG_C
Cuts at: 116 116
Size: 4691
BssSI C'ACGA_G
Cuts at: 906 1435 2962 3320 906
Size: 529 1527 358 2277
Fragments arranged by size:
2277 1527 529 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2068 2068
Size: 4691
BstBI TT'CG_AA
Cuts at: 3052 3052
Size: 4691
BstEII G'GTnAC_C
Cuts at: 1528 1528
Size: 4691
BstYI r'GATC_y
Cuts at: 1 1047 1716 2183 2206 2541 2787 3799 3885
Size: 1046 669 467 23 335 246 1012 86
Cuts at: 3885 3897 4243 1
Size: 12 346 449
Fragments arranged by size:
1046 1012 669 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 425 1232 425
Size: 807 3884
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2641 2822 2985 2641
Size: 181 163 4347
Fragments arranged by size:
4347 181 163
BtsI GCAGTG_nn'
Cuts at: 1934 1934
Size: 4691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 489 524 743 778 489
Size: 35 219 35 4402
Fragments arranged by size:
4402 219 35 35
DraI TTT'AAA
Cuts at: 1690 1690
Size: 4691
DraIII CAC_nnn'GTG
Cuts at: 603 1069 603
Size: 466 4225
DrdI GACnn_nn'nnGTC
Cuts at: 2397 3255 2397
Size: 858 3833
EaeI y'GGCC_r
Cuts at: 701 2276 2450 2841 2868 4213 701
Size: 1575 174 391 27 1345 1179
Fragments arranged by size:
1575 1345 1179 391 174 27
EagI C'GGCC_G
Cuts at: 2276 2276
Size: 4691
EarI CTCTTCn'nnn_
Cuts at: 1572 2714 2924 1572
Size: 1142 210 3339
Fragments arranged by size:
3339 1142 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2899 3209 3355 4501 2899
Size: 310 146 1146 3089
Fragments arranged by size:
3089 1146 310 146
EcoICRI GAG'CTC
Cuts at: 956 1703 956
Size: 747 3944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 834 942 1315 1368 2516 2948 3695 3963
Size: 598 108 373 53 1148 432 747 268
Cuts at: 3963 236
Size: 964
Fragments arranged by size:
1148 964 747 598 432 373 268 108
53
EcoNI CCTnn'n_nnAGG
Cuts at: 1648 1663 1648
Size: 15 4676
EcoO109I rG'GnC_Cy
Cuts at: 1830 1830
Size: 4691
EcoRI G'AATT_C
Cuts at: 1695 1695
Size: 4691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4691
FspI TGC'GCA
Cuts at: 2472 2472
Size: 4691
HaeII r_GCGC'y
Cuts at: 127 136 1124 1470 2125 2373 3395 4374 127
Size: 9 988 346 655 248 1022 979 444
Fragments arranged by size:
1022 988 979 655 444 346 248 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 633 636 665 837 869 1617 1649 2533
Size: 29 3 29 172 32 748 32 884
Cuts at: 2533 2563 2565 2595 604
Size: 30 2 30 2700
Fragments arranged by size:
2700 884 748 172 32 32 30 30
29 29 3 2
HincII GTy'rAC
Cuts at: 152 417 1730 152
Size: 265 1313 3113
Fragments arranged by size:
3113 1313 265
KasI G'GCGC_C
Cuts at: 2121 2369 2121
Size: 248 4443
KpnI G_GTAC'C
Cuts at: 1711 1711
Size: 4691
MluI A'CGCG_T
Cuts at: 4124 4124
Size: 4691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3362 3546 3362
Size: 184 4507
MscI TGG'CCA
Cuts at: 703 2452 703
Size: 1749 2942
MslI CAynn'nnrTG
Cuts at: 271 424 1149 2680 2807 271
Size: 153 725 1531 127 2155
Fragments arranged by size:
2155 1531 725 153 127
MspA1I CmG'CkG
Cuts at: 162 1494 1622 1802 2116 2476 3489 3734 162
Size: 1332 128 180 314 360 1013 245 1119
Fragments arranged by size:
1332 1119 1013 360 314 245 180 128
NaeI GCC'GGC
Cuts at: 2872 2872
Size: 4691
NarI GG'CG_CC
Cuts at: 2122 2370 2122
Size: 248 4443
NgoMIV G'CCGG_C
Cuts at: 2870 2870
Size: 4691
NspI r_CATG'y
Cuts at: 1392 1744 2244 3151 1392
Size: 352 500 907 2932
Fragments arranged by size:
2932 907 500 352
PciI A'CATG_T
Cuts at: 3147 3147
Size: 4691
PfoI T'CCnGG_A
Cuts at: 1218 1218
Size: 4691
PmeI GTTT'AAAC
Cuts at: 1690 1690
Size: 4691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4659
PshAI GACnn'nnGTC
Cuts at: 1234 1234
Size: 4691
PstI C_TGCA'G
Cuts at: 1514 1738 2423 1514
Size: 224 685 3782
Fragments arranged by size:
3782 685 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4691
PvuII CAG'CTG
Cuts at: 1622 2116 2476 1622
Size: 494 360 3837
Fragments arranged by size:
3837 494 360
RsrII CG'GwC_CG
Cuts at: 2886 2886
Size: 4691
SacI G_AGCT'C
Cuts at: 958 1705 958
Size: 747 3944
SalI G'TCGA_C
Cuts at: 1728 1728
Size: 4691
SapI GCTCTTCn'nnn_
Cuts at: 2714 2924 2714
Size: 210 4481
SbfI CC_TGCA'GG
Cuts at: 1738 1738
Size: 4691
ScaI AGT'ACT
Cuts at: 4208 4208
Size: 4691
SfcI C'TryA_G
Cuts at: 33 278 503 1510 1734 2419 3412 3603 33
Size: 245 225 1007 224 685 993 191 1121
Fragments arranged by size:
1121 1007 993 685 245 225 224 191
SfoI GGC'GCC
Cuts at: 2123 2371 2123
Size: 248 4443
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
SmaI CCC'GGG
Cuts at: 794 1713 794
Size: 919 3772
SmlI C'TyrA_G
Cuts at: 1051 1846 2761 3253 3515 1051
Size: 795 915 492 262 2227
Fragments arranged by size:
2227 915 795 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4691
SphI G_CATG'C
Cuts at: 1392 1744 2244 1392
Size: 352 500 3839
Fragments arranged by size:
3839 500 352
StyI C'CwwG_G
Cuts at: 314 354 1274 1649 1825 2179 314
Size: 40 920 375 176 354 2826
Fragments arranged by size:
2826 920 375 354 176 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2274 2407 2866 3075 2274
Size: 133 459 209 3890
Fragments arranged by size:
3890 459 209 133
TatI w'GTAC_w
Cuts at: 4206 4206
Size: 4691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 4659
Tth111I GACn'n_nGTC
Cuts at: 2488 2488
Size: 4691
XbaI T'CTAG_A
Cuts at: 1722 1722
Size: 4691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 1413 1656 2186 327
Size: 1086 243 530 2832
Fragments arranged by size:
2832 1086 530 243
XmaI C'CCGG_G
Cuts at: 792 1711 792
Size: 919 3772
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BsrGI BstXI BstZ17I Bsu36I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI