Restriction map for
pF1KE2569
(Circular) (Six-base) MAPSORT of: pF1KE2569.seq Check: 7648 from: 1 to: 6386
pF1KE2569 6386 bp
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AccI GT'mk_AC
Cuts at: 2571 3424 2571
Size: 853 5533
Acc65I G'GTAC_C
Cuts at: 3402 3402
Size: 6386
AclI AA'CG_TT
Cuts at: 6226 6226
Size: 6386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1521 1847 2410 2533 2746 3340 4211 4643
Size: 782 326 563 123 213 594 871 432
Cuts at: 4643 5390 5658 739
Size: 747 268 1467
Fragments arranged by size:
1467 871 782 747 594 563 432 326
268 213 123
AfeI AGC'GCT
Cuts at: 2964 2964
Size: 6386
AflII C'TTAA_G
Cuts at: 1725 1725
Size: 6386
AflIII A'CryG_T
Cuts at: 1199 1377 2198 3272 3738 4842 5819 1199
Size: 178 821 1074 466 1104 977 1766
Fragments arranged by size:
1766 1104 1074 977 821 466 178
AgeI A'CCGG_T
Cuts at: 4774 4774
Size: 6386
AleI CACnn'nnGTG
Cuts at: 619 3025 619
Size: 2406 3980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1038 1038
Size: 6386
AlwNI CAG_nnn'CTG
Cuts at: 681 897 1476 2061 3691 5258 681
Size: 216 579 585 1630 1567 1809
Fragments arranged by size:
1809 1630 1567 585 579 216
ApaI G_GGCC'C
Cuts at: 907 907
Size: 6386
ApaLI G'TGCA_C
Cuts at: 3276 5156 3276
Size: 1880 4506
ApoI r'AATT_y
Cuts at: 808 1112 2304 2617 2800 2899 3390 5739 5995
Size: 304 1192 313 183 99 491 2349 256
Cuts at: 5995 808
Size: 1199
Fragments arranged by size:
2349 1199 1192 491 313 304 256 183
99
AseI AT'TA_AT
Cuts at: 20 1799 2179 20
Size: 1779 380 4227
Fragments arranged by size:
4227 1779 380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
AvaI C'yCGr_G
Cuts at: 194 310 1029 2112 2995 3406 194
Size: 116 719 1083 883 411 3174
Fragments arranged by size:
3174 1083 883 719 411 116
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1673 1706 1673
Size: 33 6353
BamHI G'GATC_C
Cuts at: 3411 3411
Size: 6386
BanI G'GyrC_C
Cuts at: 128 493 3019 3402 3816 4064 4099 128
Size: 365 2526 383 414 248 35 2415
Fragments arranged by size:
2526 2415 414 383 365 248 35
BanII G_rGCy'C
Cuts at: 277 907 1061 1525 2527 2996 3147 3400 4430
Size: 630 154 464 1002 469 151 253 1030
Cuts at: 4430 277
Size: 2233
Fragments arranged by size:
2233 1030 1002 630 469 464 253 154
151
BbeI G_GCGC'C
Cuts at: 132 3820 4068 132
Size: 3688 248 2450
Fragments arranged by size:
3688 2450 248
BbsI GAAGACnn'nnnn_
Cuts at: 243 291 332 1835 2521 3328 243
Size: 48 41 1503 686 807 3301
Fragments arranged by size:
3301 1503 807 686 48 41
BciVI GTATCCnnnnn_n'
Cuts at: 463 3262 4284 5045 6386 463
Size: 2799 1022 761 1341 463
Fragments arranged by size:
2799 1341 1022 761 463
BclI T'GATC_A
Cuts at: 692 1074 1496 2399 3906 692
Size: 382 422 903 1507 3172
Fragments arranged by size:
3172 1507 903 422 382
BfrBI ATG'CAT
Cuts at: 1100 1100
Size: 6386
BglI GCCn_nnn'nGGC
Cuts at: 3000 3000
Size: 6386
BglII A'GATC_T
Cuts at: 3901 5938 3901
Size: 2037 4349
BlpI GC'TnA_GC
Cuts at: 625 2702 3498 625
Size: 2077 796 3513
Fragments arranged by size:
3513 2077 796
Bme1580I G_kGCm'C
Cuts at: 907 2972 3280 4011 4104 5160 907
Size: 2065 308 731 93 1056 2133
Fragments arranged by size:
2133 2065 1056 731 308 93
BmgBI CAC'GTC
Cuts at: 1378 2457 2820 1378
Size: 1079 363 4944
Fragments arranged by size:
4944 1079 363
BmrI ACTGGGnnnn_n'
Cuts at: 3598 3679 4014 6158 3598
Size: 81 335 2144 3826
Fragments arranged by size:
3826 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4753 4753
Size: 6386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1382 1423 2695 1256
Size: 126 41 1272 4947
Fragments arranged by size:
4947 1272 126 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 3562 4441 4933 5231 1804
Size: 1758 879 492 298 2959
Fragments arranged by size:
2959 1758 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2484 2638 6090 2484
Size: 154 3452 2780
Fragments arranged by size:
3452 2780 154
BsaAI yAC'GTr
Cuts at: 2697 3275 3739 2697
Size: 578 464 5344
Fragments arranged by size:
5344 578 464
BsaBI GATnn'nnATC
Cuts at: 6 385 2398 3900 5937 6
Size: 379 2013 1502 2037 455
Fragments arranged by size:
2037 2013 1502 455 379
BsaHI Gr'CG_yC
Cuts at: 129 578 3817 4065 4767 6263 129
Size: 449 3239 248 702 1496 252
Fragments arranged by size:
3239 1496 702 449 252 248
BsaWI w'CCGG_w
Cuts at: 2787 3208 3575 4096 4774 5048 5195 5606 2787
Size: 421 367 521 678 274 147 411 3567
Fragments arranged by size:
3567 678 521 421 411 367 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 482 486 512 516 1025 1055 2217 2247 3061
Size: 4 26 4 509 30 1162 30 814
Cuts at: 3061 3091 482
Size: 30 3777
Fragments arranged by size:
3777 1162 814 509 30 30 30 26
4 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 594 597 1447 2650 2749 3253 172
Size: 422 3 850 1203 99 504 3305
Fragments arranged by size:
3305 1203 850 504 422 99 3
BseYI C'CCAG_C
Cuts at: 1526 2971 3811 4569 5146 5642 1526
Size: 1445 840 758 577 496 2270
Fragments arranged by size:
2270 1445 840 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1978 1978
Size: 6386
BsiEI CG_ry'CG
Cuts at: 104 3928 3974 5182 104
Size: 3824 46 1208 1308
Fragments arranged by size:
3824 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 665 1061 1409 2708 2984 3147 3280 3400 4178
Size: 396 348 1299 276 163 133 120 778
Cuts at: 4178 4368 5160 665
Size: 190 792 1891
Fragments arranged by size:
1891 1299 792 778 396 348 276 190
163 133 120
BsmI GAATG_Cn'
Cuts at: 1102 1266 2339 2845 1102
Size: 164 1073 506 4643
Fragments arranged by size:
4643 1073 506 164
BsmBI CGTCTCn'nnnn_
Cuts at: 170 504 569 3235 170
Size: 334 65 2666 3321
Fragments arranged by size:
3321 2666 334 65
Bsp1286I G_dGCh'C
Cuts at: 277 665 907 1061 1409 1525 2527 2708 2972
Size: 388 242 154 348 116 1002 181 264
Cuts at: 2972 2984 2996 3147 3280 3400 4011 4104 4178
Size: 12 12 151 133 120 611 93 74
Cuts at: 4178 4368 4430 5160 277
Size: 190 62 730 1503
Fragments arranged by size:
1503 1002 730 611 388 348 264 242
190 181 154 151 133 120 116 93
74 62 12 12
BspEI T'CCGG_A
Cuts at: 2787 5606 2787
Size: 2819 3567
BspHI T'CATG_A
Cuts at: 2402 5562 2402
Size: 3160 3226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 149 3436 3650 3952 4333 149
Size: 3287 214 302 381 2202
Fragments arranged by size:
3287 2202 381 302 214
BsrBI CCG'CTC
Cuts at: 50 152 243 831 2631 3000 4678 4732 6216
Size: 102 91 588 1800 369 1678 54 1484
Cuts at: 6216 6381 50
Size: 165 55
Fragments arranged by size:
1800 1678 1484 588 369 165 102 91
55 54
BsrDI GCAATG_nn'
Cuts at: 584 1819 2071 2120 4298 584
Size: 1235 252 49 2178 2672
Fragments arranged by size:
2672 2178 1235 252 49
BsrFI r'CCGG_y
Cuts at: 852 4384 4565 4774 852
Size: 3532 181 209 2464
Fragments arranged by size:
3532 2464 209 181
BsrGI T'GTAC_A
Cuts at: 548 548
Size: 6386
BssSI C'ACGA_G
Cuts at: 1966 4657 5015 1966
Size: 2691 358 3337
Fragments arranged by size:
3337 2691 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3009 3763 3009
Size: 754 5632
BstBI TT'CG_AA
Cuts at: 4747 4747
Size: 6386
BstEII G'GTnAC_C
Cuts at: 3231 3231
Size: 6386
BstXI CCAn_nnnn'nTGG
Cuts at: 1353 2501 3198 1353
Size: 1148 697 4541
Fragments arranged by size:
4541 1148 697
BstYI r'GATC_y
Cuts at: 1 677 2144 3411 3878 3901 4236 4482 5494
Size: 676 1467 1267 467 23 335 246 1012
Cuts at: 5494 5580 5592 5938 1
Size: 86 12 346 449
Fragments arranged by size:
1467 1267 1012 676 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 187 464 866 1682 1694 1889 2140 2519 2648
Size: 277 402 816 12 195 251 379 129
Cuts at: 2648 3023 3114 187
Size: 375 91 3459
Fragments arranged by size:
3459 816 402 379 375 277 251 195
129 91 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 136 846 2850 4336 4517 4680 136
Size: 710 2004 1486 181 163 1842
Fragments arranged by size:
2004 1842 1486 710 181 163
BtsI GCAGTG_nn'
Cuts at: 1846 1894 2554 3629 1846
Size: 48 660 1075 4603
Fragments arranged by size:
4603 1075 660 48
DraI TTT'AAA
Cuts at: 1466 3385 1466
Size: 1919 4467
DraIII CAC_nnn'GTG
Cuts at: 1479 1479
Size: 6386
DrdI GACnn_nn'nnGTC
Cuts at: 4092 4950 4092
Size: 858 5528
EaeI y'GGCC_r
Cuts at: 683 1343 2137 2186 2714 3188 3971 4145 4536
Size: 660 794 49 528 474 783 174 391
Cuts at: 4536 4563 5908 683
Size: 27 1345 1161
Fragments arranged by size:
1345 1161 794 783 660 528 474 391
174 49 27
EagI C'GGCC_G
Cuts at: 3971 3971
Size: 6386
EarI CTCTTCn'nnn_
Cuts at: 436 535 758 1540 1741 2041 4409 4619 436
Size: 99 223 782 201 300 2368 210 2203
Fragments arranged by size:
2368 2203 782 300 223 210 201 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 188 4594 4904 5050 6196 188
Size: 4406 310 146 1146 378
Fragments arranged by size:
4406 1146 378 310 146
EcoICRI GAG'CTC
Cuts at: 1059 3145 3398 1059
Size: 2086 253 4047
Fragments arranged by size:
4047 2086 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1256 1382 1423 1521 1847 2410 2533 2695
Size: 517 126 41 98 326 563 123 162
Cuts at: 2695 2746 3340 4211 4643 5390 5658 739
Size: 51 594 871 432 747 268 1467
Fragments arranged by size:
1467 871 747 594 563 517 432 326
268 162 126 123 98 51 41
EcoO109I rG'GnC_Cy
Cuts at: 903 904 1368 1455 1574 3315 3353 3525 903
Size: 1 464 87 119 1741 38 172 3764
Fragments arranged by size:
3764 1741 464 172 119 87 38 1
EcoRI G'AATT_C
Cuts at: 3390 3390
Size: 6386
EcoRV GAT'ATC
Cuts at: 1245 2354 3165 1245
Size: 1109 811 4466
Fragments arranged by size:
4466 1109 811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2850 3096 3345 4405 2850
Size: 246 249 1060 4831
Fragments arranged by size:
4831 1060 249 246
FspI TGC'GCA
Cuts at: 2300 4167 2300
Size: 1867 4519
HaeII r_GCGC'y
Cuts at: 132 1421 2966 3820 4068 5090 6069 132
Size: 1289 1545 854 248 1022 979 449
Fragments arranged by size:
1545 1289 1022 979 854 449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 482 502 514 534 566 598 1025 1057 1229
Size: 20 12 20 32 32 427 32 172
Cuts at: 1229 1261 1309 1341 3129 3161 3278 3310 4228
Size: 32 48 32 1788 32 117 32 918
Cuts at: 4228 4258 4260 4290 482
Size: 30 2 30 2578
Fragments arranged by size:
2578 1788 918 427 172 117 48 32
32 32 32 32 32 32 30 30
20 20 12 2
HincII GTy'rAC
Cuts at: 823 3425 823
Size: 2602 3784
HindIII A'AGCT_T
Cuts at: 2259 2259
Size: 6386
KasI G'GCGC_C
Cuts at: 128 3816 4064 128
Size: 3688 248 2450
Fragments arranged by size:
3688 2450 248
KpnI G_GTAC'C
Cuts at: 3406 3406
Size: 6386
MfeI C'AATT_G
Cuts at: 1787 1787
Size: 6386
MluI A'CGCG_T
Cuts at: 5819 5819
Size: 6386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 379 785 2260 2840 5057 5241 379
Size: 406 1475 580 2217 184 1524
Fragments arranged by size:
2217 1524 1475 580 406 184
MscI TGG'CCA
Cuts at: 685 1345 2139 2716 3190 4147 685
Size: 660 794 577 474 957 2924
Fragments arranged by size:
2924 957 794 660 577 474
MslI CAynn'nnrTG
Cuts at: 268 541 619 865 871 1255 1412 2179 2407
Size: 273 78 246 6 384 157 767 228
Cuts at: 2407 2941 3025 3343 4375 4502 268
Size: 534 84 318 1032 127 2152
Fragments arranged by size:
2152 1032 767 534 384 318 273 246
228 157 127 84 78 6
MspA1I CmG'CkG
Cuts at: 189 2336 3497 3811 4171 5184 5429 189
Size: 2147 1161 314 360 1013 245 1146
Fragments arranged by size:
2147 1161 1146 1013 360 314 245
NaeI GCC'GGC
Cuts at: 854 4567 854
Size: 3713 2673
NarI GG'CG_CC
Cuts at: 129 3817 4065 129
Size: 3688 248 2450
Fragments arranged by size:
3688 2450 248
NcoI C'CATG_G
Cuts at: 464 866 1694 2519 2648 3023 3114 464
Size: 402 828 825 129 375 91 3736
Fragments arranged by size:
3736 828 825 402 375 129 91
NgoMIV G'CCGG_C
Cuts at: 852 4565 852
Size: 3713 2673
NsiI A_TGCA'T
Cuts at: 1102 1102
Size: 6386
NspI r_CATG'y
Cuts at: 573 1050 1203 1254 2202 3012 3439 3939 4846
Size: 477 153 51 948 810 427 500 907
Cuts at: 4846 573
Size: 2113
Fragments arranged by size:
2113 948 907 810 500 477 427 153
51
PasI CC'CwG_GG
Cuts at: 506 506
Size: 6386
PciI A'CATG_T
Cuts at: 1199 2198 4842 1199
Size: 999 2644 2743
Fragments arranged by size:
2743 2644 999
PflMI CCAn_nnn'nTGG
Cuts at: 2460 2460
Size: 6386
PfoI T'CCnGG_A
Cuts at: 3215 3215
Size: 6386
PmeI GTTT'AAAC
Cuts at: 3385 3385
Size: 6386
PmlI CAC'GTG
Cuts at: 2697 3275 2697
Size: 578 5808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5525 5557 5525
Size: 32 6354
PpuMI rG'GwC_Cy
Cuts at: 1368 1455 3315 3353 1368
Size: 87 1860 38 4401
Fragments arranged by size:
4401 1860 87 38
PsiI TTA'TAA
Cuts at: 1424 1424
Size: 6386
PspOMI G'GGCC_C
Cuts at: 903 903
Size: 6386
PstI C_TGCA'G
Cuts at: 902 1304 3260 3433 4118 902
Size: 402 1956 173 685 3170
Fragments arranged by size:
3170 1956 685 402 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6386
PvuII CAG'CTG
Cuts at: 2336 3811 4171 2336
Size: 1475 360 4551
Fragments arranged by size:
4551 1475 360
RsrII CG'GwC_CG
Cuts at: 1581 4581 1581
Size: 3000 3386
SacI G_AGCT'C
Cuts at: 1061 3147 3400 1061
Size: 2086 253 4047
Fragments arranged by size:
4047 2086 253
SacII CC_GC'GG
Cuts at: 190 190
Size: 6386
SalI G'TCGA_C
Cuts at: 3423 3423
Size: 6386
SanDI GG'GwC_CC
Cuts at: 1368 1455 1368
Size: 87 6299
SapI GCTCTTCn'nnn_
Cuts at: 758 4409 4619 758
Size: 3651 210 2525
Fragments arranged by size:
3651 2525 210
SbfI CC_TGCA'GG
Cuts at: 3433 3433
Size: 6386
ScaI AGT'ACT
Cuts at: 1868 5903 1868
Size: 4035 2351
SexAI A'CCwGG_T
Cuts at: 512 665 1475 512
Size: 153 810 5423
Fragments arranged by size:
5423 810 153
SfcI C'TryA_G
Cuts at: 33 898 1300 2331 2469 2589 3256 3429 4114
Size: 865 402 1031 138 120 667 173 685
Cuts at: 4114 5107 5298 33
Size: 993 191 1121
Fragments arranged by size:
1121 1031 993 865 685 667 402 191
173 138 120
SfoI GGC'GCC
Cuts at: 130 3818 4066 130
Size: 3688 248 2450
Fragments arranged by size:
3688 2450 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
SmaI CCC'GGG
Cuts at: 196 1031 3408 196
Size: 835 2377 3174
Fragments arranged by size:
3174 2377 835
SmlI C'TyrA_G
Cuts at: 1725 1819 3541 4456 4948 5210 1725
Size: 94 1722 915 492 262 2901
Fragments arranged by size:
2901 1722 915 492 262 94
SpeI A'CTAG_T
Cuts at: 67 1038 67
Size: 971 5415
SphI G_CATG'C
Cuts at: 3439 3939 3439
Size: 500 5886
SspI AAT'ATT
Cuts at: 2584 2584
Size: 6386
StyI C'CwwG_G
Cuts at: 464 866 908 1340 1505 1694 2414 2519 2648
Size: 402 42 432 165 189 720 105 129
Cuts at: 2648 3023 3114 3520 3874 464
Size: 375 91 406 354 2976
Fragments arranged by size:
2976 720 432 406 402 375 354 189
165 129 105 91 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1569 2461 3969 4102 4561 4770 1569
Size: 892 1508 133 459 209 3185
Fragments arranged by size:
3185 1508 892 459 209 133
TatI w'GTAC_w
Cuts at: 548 1866 3005 5901 548
Size: 1318 1139 2896 1033
Fragments arranged by size:
2896 1318 1139 1033
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 486 518 5903 5935 486
Size: 32 5385 32 937
Fragments arranged by size:
5385 937 32 32
Tth111I GACn'n_nGTC
Cuts at: 2761 4183 2761
Size: 1422 4964
XbaI T'CTAG_A
Cuts at: 3417 3417
Size: 6386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1357 1555 3881 1357
Size: 198 2326 3862
Fragments arranged by size:
3862 2326 198
XmaI C'CCGG_G
Cuts at: 194 1029 3406 194
Size: 835 2377 3174
Fragments arranged by size:
3174 2377 835
XmnI GAAnn'nnTTC
Cuts at: 1832 2617 2843 1832
Size: 785 226 5375
Fragments arranged by size:
5375 785 226
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AloI AscI AvrII BbvCI BcgI
BmtI Bpu10I BsiWI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI FseI FspAI HpaI NdeI NheI NotI NruI
PacI PshAI PspXI PsrI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI