Restriction map for
pF1KE2567
(Circular) (Six-base) MAPSORT of: pF1KE2567.seq Check: 3345 from: 1 to: 5342
pF1KE2567 5342 bp
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 402 402
Size: 5342
AccI GT'mk_AC
Cuts at: 2333 2380 2333
Size: 47 5295
Acc65I G'GTAC_C
Cuts at: 879 2358 879
Size: 1479 3863
AclI AA'CG_TT
Cuts at: 5182 5182
Size: 5342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1149 1977 2158 3167 3599 4346 4614 196
Size: 953 828 181 1009 432 747 268 924
Fragments arranged by size:
1009 953 924 828 747 432 268 181
AflIII A'CryG_T
Cuts at: 1373 2694 3798 4775 1373
Size: 1321 1104 977 1940
Fragments arranged by size:
1940 1321 1104 977
AgeI A'CCGG_T
Cuts at: 3730 3730
Size: 5342
AhdI GACnn_n'nnGTC
Cuts at: 286 286
Size: 5342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1657 1657
Size: 5342
AlwNI CAG_nnn'CTG
Cuts at: 492 2647 4214 492
Size: 2155 1567 1620
Fragments arranged by size:
2155 1620 1567
ApaI G_GGCC'C
Cuts at: 1058 1058
Size: 5342
ApaLI G'TGCA_C
Cuts at: 4112 4112
Size: 5342
ApoI r'AATT_y
Cuts at: 199 2346 4695 4951 199
Size: 2147 2349 256 590
Fragments arranged by size:
2349 2147 590 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5342
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5342
AvaI C'yCGr_G
Cuts at: 669 1051 2298 2362 669
Size: 382 1247 64 3649
Fragments arranged by size:
3649 1247 382 64
BamHI G'GATC_C
Cuts at: 2367 2367
Size: 5342
BanI G'GyrC_C
Cuts at: 125 879 1707 1879 2358 2772 3020 3055 125
Size: 754 828 172 479 414 248 35 2412
Fragments arranged by size:
2412 828 754 479 414 248 172 35
BanII G_rGCy'C
Cuts at: 317 585 669 848 974 1058 1132 1177 2356
Size: 268 84 179 126 84 74 45 1179
Cuts at: 2356 3386 317
Size: 1030 2273
Fragments arranged by size:
2273 1179 1030 268 179 126 84 84
74 45
BbeI G_GCGC'C
Cuts at: 1711 1883 2776 3024 1711
Size: 172 893 248 4029
Fragments arranged by size:
4029 893 248 172
BbsI GAAGACnn'nnnn_
Cuts at: 271 803 271
Size: 532 4810
BbvCI CC'TCA_GC
Cuts at: 1743 1743
Size: 5342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 671 705 671
Size: 34 5308
BciVI GTATCCnnnnn_n'
Cuts at: 3240 4001 5342 3240
Size: 761 1341 3240
Fragments arranged by size:
3240 1341 761
BclI T'GATC_A
Cuts at: 2862 2862
Size: 5342
BfrBI ATG'CAT
Cuts at: 330 330
Size: 5342
BglI GCCn_nnn'nGGC
Cuts at: 931 931
Size: 5342
BglII A'GATC_T
Cuts at: 176 2857 4894 176
Size: 2681 2037 624
Fragments arranged by size:
2681 2037 624
BlpI GC'TnA_GC
Cuts at: 599 2454 599
Size: 1855 3487
Bme1580I G_kGCm'C
Cuts at: 518 1058 1211 2967 3060 4116 518
Size: 540 153 1756 93 1056 1744
Fragments arranged by size:
1756 1744 1056 540 153 93
BmgBI CAC'GTC
Cuts at: 1160 1560 2082 1160
Size: 400 522 4420
Fragments arranged by size:
4420 522 400
BmrI ACTGGGnnnn_n'
Cuts at: 2554 2635 2970 5114 2554
Size: 81 335 2144 2782
Fragments arranged by size:
2782 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 701 2262 3709 701
Size: 1561 1447 2334
Fragments arranged by size:
2334 1561 1447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 403 469 599 2220 2253 124
Size: 279 66 130 1621 33 3213
Fragments arranged by size:
3213 1621 279 130 66 33
Bpu10I CC'TnA_GC
Cuts at: 1743 1743
Size: 5342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 2518 3397 3889 4187 217
Size: 2301 879 492 298 1372
Fragments arranged by size:
2301 1372 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1190 5046 1190
Size: 3856 1486
BsaAI yAC'GTr
Cuts at: 1374 2695 1374
Size: 1321 4021
BsaBI GATnn'nnATC
Cuts at: 6 812 2856 4893 6
Size: 806 2044 2037 455
Fragments arranged by size:
2044 2037 806 455
BsaHI Gr'CG_yC
Cuts at: 1582 1708 1781 1880 2206 2773 3021 3723 5219
Size: 126 73 99 326 567 248 702 1496
Cuts at: 5219 1582
Size: 1705
Fragments arranged by size:
1705 1496 702 567 326 248 126 99
73
BsaWI w'CCGG_w
Cuts at: 2531 3052 3730 4004 4151 4562 2531
Size: 521 678 274 147 411 3311
Fragments arranged by size:
3311 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1255 1285 1981 2011 1255
Size: 30 696 30 4586
Fragments arranged by size:
4586 696 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 727 730 1014 1140 727
Size: 3 284 126 4929
Fragments arranged by size:
4929 284 126 3
BseYI C'CCAG_C
Cuts at: 531 850 1057 1210 1336 1592 1749 1952 2767
Size: 319 207 153 126 256 157 203 815
Cuts at: 2767 3525 4102 4598 531
Size: 758 577 496 1275
Fragments arranged by size:
1275 815 758 577 496 319 256 207
203 157 153 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2062 2092 1444
Size: 618 30 4694
Fragments arranged by size:
4694 618 30
BsiEI CG_ry'CG
Cuts at: 104 186 724 2048 2884 2930 4138 104
Size: 82 538 1324 836 46 1208 1308
Fragments arranged by size:
1324 1308 1208 836 538 82 46
BsiHKAI G_wGCw'C
Cuts at: 419 974 2173 2356 3134 3324 4116 419
Size: 555 1199 183 778 190 792 1645
Fragments arranged by size:
1645 1199 792 778 555 190 183
BsiWI C'GTAC_G
Cuts at: 308 2210 308
Size: 1902 3440
BsmI GAATG_Cn'
Cuts at: 890 890
Size: 5342
BsmBI CGTCTCn'nnnn_
Cuts at: 1326 1326
Size: 5342
Bsp1286I G_dGCh'C
Cuts at: 317 419 518 585 669 848 974 1058 1132
Size: 102 99 67 84 179 126 84 74
Cuts at: 1132 1177 1211 2173 2356 2967 3060 3134 3324
Size: 45 34 962 183 611 93 74 190
Cuts at: 3324 3386 4116 317
Size: 62 730 1543
Fragments arranged by size:
1543 962 730 611 190 183 179 126
102 99 93 84 84 74 74 67
62 45 34
BspEI T'CCGG_A
Cuts at: 4562 4562
Size: 5342
BspHI T'CATG_A
Cuts at: 4518 4518
Size: 5342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 402 432 1841 1967 2195 2392 2606 2908 3289
Size: 30 1409 126 228 197 214 302 381
Cuts at: 3289 402
Size: 2455
Fragments arranged by size:
2455 1409 381 302 228 214 197 126
30
BsrBI CCG'CTC
Cuts at: 50 138 362 3634 3688 5172 5337 50
Size: 88 224 3272 54 1484 165 55
Fragments arranged by size:
3272 1484 224 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 3254 3254
Size: 5342
BsrFI r'CCGG_y
Cuts at: 1356 1398 3340 3521 3730 1356
Size: 42 1942 181 209 2968
Fragments arranged by size:
2968 1942 209 181 42
BsrGI T'GTAC_A
Cuts at: 1523 1523
Size: 5342
BssSI C'ACGA_G
Cuts at: 706 2116 3613 3971 706
Size: 1410 1497 358 2077
Fragments arranged by size:
2077 1497 1410 358
BstAPI GCAn_nnn'nTGC
Cuts at: 414 2719 414
Size: 2305 3037
BstBI TT'CG_AA
Cuts at: 3703 3703
Size: 5342
BstEII G'GTnAC_C
Cuts at: 444 1494 444
Size: 1050 4292
BstYI r'GATC_y
Cuts at: 1 176 2072 2367 2834 2857 3192 3438 4450
Size: 175 1896 295 467 23 335 246 1012
Cuts at: 4450 4536 4548 4894 1
Size: 86 12 346 449
Fragments arranged by size:
1896 1012 467 449 346 335 295 246
175 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1123 1123
Size: 5342
BtgI C'CryG_G
Cuts at: 449 1106 1441 1473 1586 449
Size: 657 335 32 113 4205
Fragments arranged by size:
4205 657 335 113 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1823 3292 3473 3636 1823
Size: 1469 181 163 3529
Fragments arranged by size:
3529 1469 181 163
BtsI GCAGTG_nn'
Cuts at: 558 2019 2585 558
Size: 1461 566 3315
Fragments arranged by size:
3315 1461 566
DraI TTT'AAA
Cuts at: 2341 2341
Size: 5342
DrdI GACnn_nn'nnGTC
Cuts at: 3048 3906 3048
Size: 858 4484
EaeI y'GGCC_r
Cuts at: 147 254 1064 1650 1766 2927 3101 3492 3519
Size: 107 810 586 116 1161 174 391 27
Cuts at: 3519 4864 147
Size: 1345 625
Fragments arranged by size:
1345 1161 810 625 586 391 174 116
107 27
EagI C'GGCC_G
Cuts at: 2927 2927
Size: 5342
EarI CTCTTCn'nnn_
Cuts at: 1329 3365 3575 1329
Size: 2036 210 3096
Fragments arranged by size:
3096 2036 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 355 3550 3860 4006 5152 355
Size: 3195 310 146 1146 545
Fragments arranged by size:
3195 1146 545 310 146
EcoICRI GAG'CTC
Cuts at: 972 2354 972
Size: 1382 3960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 196 403 469 599 1149 1977 2158 2220
Size: 72 207 66 130 550 828 181 62
Cuts at: 2220 2253 3167 3599 4346 4614 124
Size: 33 914 432 747 268 852
Fragments arranged by size:
914 852 828 747 550 432 268 207
181 130 72 66 62 33
EcoNI CCTnn'n_nnAGG
Cuts at: 394 1963 394
Size: 1569 3773
EcoO109I rG'GnC_Cy
Cuts at: 778 906 1698 1830 1929 1956 2481 778
Size: 128 792 132 99 27 525 3639
Fragments arranged by size:
3639 792 525 132 128 99 27
EcoRI G'AATT_C
Cuts at: 199 2346 199
Size: 2147 3195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1809 3361 1809
Size: 1552 3790
FspI TGC'GCA
Cuts at: 3123 3123
Size: 5342
HaeII r_GCGC'y
Cuts at: 1711 1883 2776 3024 4046 5025 1711
Size: 172 893 248 1022 979 2028
Fragments arranged by size:
2028 1022 979 893 248 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 272 304 823 855 1981 2013 3184 3214 3216
Size: 32 519 32 1126 32 1171 30 2
Cuts at: 3216 3246 272
Size: 30 2368
Fragments arranged by size:
2368 1171 1126 519 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2381 2381
Size: 5342
KasI G'GCGC_C
Cuts at: 1707 1879 2772 3020 1707
Size: 172 893 248 4029
Fragments arranged by size:
4029 893 248 172
KpnI G_GTAC'C
Cuts at: 883 2362 883
Size: 1479 3863
MluI A'CGCG_T
Cuts at: 4775 4775
Size: 5342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2028 4013 4197 2028
Size: 1985 184 3173
Fragments arranged by size:
3173 1985 184
MscI TGG'CCA
Cuts at: 256 3103 256
Size: 2847 2495
MslI CAynn'nnrTG
Cuts at: 325 3331 3458 325
Size: 3006 127 2209
Fragments arranged by size:
3006 2209 127
MspA1I CmG'CkG
Cuts at: 492 603 954 1443 1458 1505 1886 1977 2106
Size: 111 351 489 15 47 381 91 129
Cuts at: 2106 2453 2767 3127 4140 4385 492
Size: 347 314 360 1013 245 1449
Fragments arranged by size:
1449 1013 489 381 360 351 347 314
245 129 111 91 47 15
NaeI GCC'GGC
Cuts at: 1358 3523 1358
Size: 2165 3177
NarI GG'CG_CC
Cuts at: 1708 1880 2773 3021 1708
Size: 172 893 248 4029
Fragments arranged by size:
4029 893 248 172
NcoI C'CATG_G
Cuts at: 1473 1473
Size: 5342
NgoMIV G'CCGG_C
Cuts at: 1356 3521 1356
Size: 2165 3177
NsiI A_TGCA'T
Cuts at: 332 332
Size: 5342
NspI r_CATG'y
Cuts at: 1522 2395 2895 3802 1522
Size: 873 500 907 3062
Fragments arranged by size:
3062 907 873 500
PasI CC'CwG_GG
Cuts at: 117 1345 2013 117
Size: 1228 668 3446
Fragments arranged by size:
3446 1228 668
PciI A'CATG_T
Cuts at: 3798 3798
Size: 5342
PmeI GTTT'AAAC
Cuts at: 2341 2341
Size: 5342
PmlI CAC'GTG
Cuts at: 1374 1374
Size: 5342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 4481
Size: 32 5310
PpuMI rG'GwC_Cy
Cuts at: 778 906 1698 1830 1956 778
Size: 128 792 132 126 4164
Fragments arranged by size:
4164 792 132 128 126
PshAI GACnn'nnGTC
Cuts at: 1849 1849
Size: 5342
PspOMI G'GGCC_C
Cuts at: 1054 1054
Size: 5342
PstI C_TGCA'G
Cuts at: 1807 1838 1982 2389 3074 1807
Size: 31 144 407 685 4075
Fragments arranged by size:
4075 685 407 144 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5342
PvuII CAG'CTG
Cuts at: 603 1458 1886 1977 2106 2767 3127 603
Size: 855 428 91 129 661 360 2818
Fragments arranged by size:
2818 855 661 428 360 129 91
RsrII CG'GwC_CG
Cuts at: 3537 3537
Size: 5342
SacI G_AGCT'C
Cuts at: 974 2356 974
Size: 1382 3960
SacII CC_GC'GG
Cuts at: 1444 1444
Size: 5342
SalI G'TCGA_C
Cuts at: 2379 2379
Size: 5342
SapI GCTCTTCn'nnn_
Cuts at: 3365 3575 3365
Size: 210 5132
SbfI CC_TGCA'GG
Cuts at: 2389 2389
Size: 5342
ScaI AGT'ACT
Cuts at: 4859 4859
Size: 5342
SexAI A'CCwGG_T
Cuts at: 908 908
Size: 5342
SfcI C'TryA_G
Cuts at: 33 1803 1834 1978 2385 3070 4063 4254 33
Size: 1770 31 144 407 685 993 191 1121
Fragments arranged by size:
1770 1121 993 685 407 191 144 31
SfoI GGC'GCC
Cuts at: 1709 1881 2774 3022 1709
Size: 172 893 248 4029
Fragments arranged by size:
4029 893 248 172
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5342
SmaI CCC'GGG
Cuts at: 1053 2364 1053
Size: 1311 4031
SmlI C'TyrA_G
Cuts at: 232 2497 3412 3904 4166 232
Size: 2265 915 492 262 1408
Fragments arranged by size:
2265 1408 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5342
SphI G_CATG'C
Cuts at: 2395 2895 2395
Size: 500 4842
StyI C'CwwG_G
Cuts at: 929 942 965 1473 1784 2234 2476 2830 929
Size: 13 23 508 311 450 242 354 3441
Fragments arranged by size:
3441 508 450 354 311 242 23 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 738 2925 3058 3517 3726 738
Size: 2187 133 459 209 2354
Fragments arranged by size:
2354 2187 459 209 133
TatI w'GTAC_w
Cuts at: 1523 4857 1523
Size: 3334 2008
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4859 4891 4859
Size: 32 5310
Tth111I GACn'n_nGTC
Cuts at: 1114 1140 3139 1114
Size: 26 1999 3317
Fragments arranged by size:
3317 1999 26
XbaI T'CTAG_A
Cuts at: 2373 2373
Size: 5342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1584 1698 2837 1584
Size: 114 1139 4089
Fragments arranged by size:
4089 1139 114
XmaI C'CCGG_G
Cuts at: 1051 2362 1051
Size: 1311 4031
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BmtI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PflMI PfoI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI