Restriction map for
pF1KE2564
(Circular) (Six-base) MAPSORT of: pF1KE2564.seq Check: 7081 from: 1 to: 4937
pF1KE2564 4937 bp
With 182 enzymes: SgfI *
March 13, 2015 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1210 1210
Size: 4937
AccI GT'mk_AC
Cuts at: 1975 1975
Size: 4937
Acc65I G'GTAC_C
Cuts at: 1953 1953
Size: 4937
AclI AA'CG_TT
Cuts at: 4777 4777
Size: 4937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1431 1854 2762 3194 3941 4209 661
Size: 770 423 908 432 747 268 1389
Fragments arranged by size:
1389 908 770 747 432 423 268
AflIII A'CryG_T
Cuts at: 2289 3393 4370 2289
Size: 1104 977 2856
Fragments arranged by size:
2856 1104 977
AgeI A'CCGG_T
Cuts at: 3325 3325
Size: 4937
AleI CACnn'nnGTG
Cuts at: 785 1555 1720 1859 785
Size: 770 165 139 3863
Fragments arranged by size:
3863 770 165 139
AlwNI CAG_nnn'CTG
Cuts at: 125 506 1156 2242 3809 125
Size: 381 650 1086 1567 1253
Fragments arranged by size:
1567 1253 1086 650 381
ApaI G_GGCC'C
Cuts at: 205 989 205
Size: 784 4153
ApaLI G'TGCA_C
Cuts at: 1843 3707 1843
Size: 1864 3073
ApoI r'AATT_y
Cuts at: 1941 4290 4546 1941
Size: 2349 256 2332
Fragments arranged by size:
2349 2332 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4937
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
AvaI C'yCGr_G
Cuts at: 485 731 1957 485
Size: 246 1226 3465
Fragments arranged by size:
3465 1226 246
BamHI G'GATC_C
Cuts at: 920 1962 920
Size: 1042 3895
BanI G'GyrC_C
Cuts at: 307 607 1189 1463 1549 1953 2367 2615 2650
Size: 300 582 274 86 404 414 248 35
Cuts at: 2650 307
Size: 2594
Fragments arranged by size:
2594 582 414 404 300 274 248 86
35
BanII G_rGCy'C
Cuts at: 139 205 815 989 1951 2981 139
Size: 66 610 174 962 1030 2095
Fragments arranged by size:
2095 1030 962 610 174 66
BbeI G_GCGC'C
Cuts at: 611 1467 2371 2619 611
Size: 856 904 248 2929
Fragments arranged by size:
2929 904 856 248
BbsI GAAGACnn'nnnn_
Cuts at: 1313 1598 1313
Size: 285 4652
BbvCI CC'TCA_GC
Cuts at: 851 1562 851
Size: 711 4226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 505 539 90
Size: 34 381 34 4488
Fragments arranged by size:
4488 381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2835 3596 4937 2835
Size: 761 1341 2835
Fragments arranged by size:
2835 1341 761
BclI T'GATC_A
Cuts at: 2457 2457
Size: 4937
BglI GCCn_nnn'nGGC
Cuts at: 557 1042 557
Size: 485 4452
BglII A'GATC_T
Cuts at: 1260 2452 4489 1260
Size: 1192 2037 1708
Fragments arranged by size:
2037 1708 1192
BlpI GC'TnA_GC
Cuts at: 1362 1371 2049 1362
Size: 9 678 4250
Fragments arranged by size:
4250 678 9
Bme1580I G_kGCm'C
Cuts at: 205 310 661 832 989 1182 1847 2562 2655
Size: 105 351 171 157 193 665 715 93
Cuts at: 2655 3711 205
Size: 1056 1431
Fragments arranged by size:
1431 1056 715 665 351 193 171 157
105 93
BmrI ACTGGGnnnn_n'
Cuts at: 914 1029 1066 1110 2149 2230 2565 4709 914
Size: 115 37 44 1039 81 335 2144 1142
Fragments arranged by size:
2144 1142 1039 335 115 81 44 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 767 1632 3304 767
Size: 865 1672 2400
Fragments arranged by size:
2400 1672 865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 1051 1253 1729 638
Size: 413 202 476 3846
Fragments arranged by size:
3846 476 413 202
Bpu10I CC'TnA_GC
Cuts at: 851 1562 851
Size: 711 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 2113 2992 3484 3782 462
Size: 1651 879 492 298 1617
Fragments arranged by size:
1651 1617 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 188 907 1084 1285 4641 188
Size: 719 177 201 3356 484
Fragments arranged by size:
3356 719 484 201 177
BsaAI yAC'GTr
Cuts at: 2290 2290
Size: 4937
BsaBI GATnn'nnATC
Cuts at: 6 1711 2451 4488 6
Size: 1705 740 2037 455
Fragments arranged by size:
2037 1705 740 455
BsaHI Gr'CG_yC
Cuts at: 608 1398 1464 2368 2616 3318 4814 608
Size: 790 66 904 248 702 1496 731
Fragments arranged by size:
1496 904 790 731 702 248 66
BsaWI w'CCGG_w
Cuts at: 884 2126 2647 3325 3599 3746 4157 884
Size: 1242 521 678 274 147 411 1664
Fragments arranged by size:
1664 1242 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1081 1111 1081
Size: 30 4907
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1404 1404
Size: 4937
BseYI C'CCAG_C
Cuts at: 363 491 526 714 995 1459 1670 2362 3120
Size: 128 35 188 281 464 211 692 758
Cuts at: 3120 3697 4193 363
Size: 577 496 1107
Fragments arranged by size:
1107 758 692 577 496 464 281 211
188 128 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 1131 1255 1306 186
Size: 945 124 51 3817
Fragments arranged by size:
3817 945 124 51
BsiEI CG_ry'CG
Cuts at: 104 2479 2525 3733 104
Size: 2375 46 1208 1308
Fragments arranged by size:
2375 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 732 815 1847 1951 2729 2919 3711 732
Size: 83 1032 104 778 190 792 1958
Fragments arranged by size:
1958 1032 792 778 190 104 83
Bsp1286I G_dGCh'C
Cuts at: 139 205 310 661 732 815 832 989 1182
Size: 66 105 351 71 83 17 157 193
Cuts at: 1182 1847 1951 2562 2655 2729 2919 2981 3711
Size: 665 104 611 93 74 190 62 730
Cuts at: 3711 139
Size: 1365
Fragments arranged by size:
1365 730 665 611 351 193 190 157
105 104 93 83 74 71 66 62
17
BspEI T'CCGG_A
Cuts at: 4157 4157
Size: 4937
BspHI T'CATG_A
Cuts at: 4113 4113
Size: 4937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 1210 1987 2201 2503 2884 692
Size: 518 777 214 302 381 2745
Fragments arranged by size:
2745 777 518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1370 3229 3283 4767 4932 50
Size: 1320 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 1320 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 2849 1808
Size: 1041 3896
BsrFI r'CCGG_y
Cuts at: 1310 1394 1682 2935 3116 3325 1310
Size: 84 288 1253 181 209 2922
Fragments arranged by size:
2922 1253 288 209 181 84
BssSI C'ACGA_G
Cuts at: 1483 3208 3566 1483
Size: 1725 358 2854
Fragments arranged by size:
2854 1725 358
BstAPI GCAn_nnn'nTGC
Cuts at: 173 314 2314 173
Size: 141 2000 2796
Fragments arranged by size:
2796 2000 141
BstBI TT'CG_AA
Cuts at: 3298 3298
Size: 4937
BstEII G'GTnAC_C
Cuts at: 1390 1390
Size: 4937
BstYI r'GATC_y
Cuts at: 1 920 1260 1421 1674 1962 2429 2452 2787
Size: 919 340 161 253 288 467 23 335
Cuts at: 2787 3033 4045 4131 4143 4489 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 919 467 449 346 340 335 288
253 246 161 86 23 12
BtgI C'CryG_G
Cuts at: 197 1037 1077 1491 197
Size: 840 40 414 3643
Fragments arranged by size:
3643 840 414 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 332 2887 3068 3231 332
Size: 2555 181 163 2038
Fragments arranged by size:
2555 2038 181 163
BtsI GCAGTG_nn'
Cuts at: 136 456 1226 2180 136
Size: 320 770 954 2893
Fragments arranged by size:
2893 954 770 320
DraI TTT'AAA
Cuts at: 1936 1936
Size: 4937
DraIII CAC_nnn'GTG
Cuts at: 1601 1601
Size: 4937
DrdI GACnn_nn'nnGTC
Cuts at: 574 1161 2643 3501 574
Size: 587 1482 858 2010
Fragments arranged by size:
2010 1482 858 587
EaeI y'GGCC_r
Cuts at: 1355 1908 2522 2696 3087 3114 4459 1355
Size: 553 614 174 391 27 1345 1833
Fragments arranged by size:
1833 1345 614 553 391 174 27
EagI C'GGCC_G
Cuts at: 2522 2522
Size: 4937
EarI CTCTTCn'nnn_
Cuts at: 1337 1871 2960 3170 1337
Size: 534 1089 210 3104
Fragments arranged by size:
3104 1089 534 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3145 3455 3601 4747 3145
Size: 310 146 1146 3335
Fragments arranged by size:
3335 1146 310 146
EcoICRI GAG'CTC
Cuts at: 813 1949 813
Size: 1136 3801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 661 1051 1253 1431 1729 1854 2762 3194
Size: 23 390 202 178 298 125 908 432
Cuts at: 3194 3941 4209 638
Size: 747 268 1366
Fragments arranged by size:
1366 908 747 432 390 298 268 202
178 125 23
EcoNI CCTnn'n_nnAGG
Cuts at: 416 1206 416
Size: 790 4147
EcoO109I rG'GnC_Cy
Cuts at: 202 681 979 986 999 1025 1217 2076 202
Size: 479 298 7 13 26 192 859 3063
Fragments arranged by size:
3063 859 479 298 192 26 13 7
EcoRI G'AATT_C
Cuts at: 1941 1941
Size: 4937
EcoRV GAT'ATC
Cuts at: 1533 1533
Size: 4937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4937
FspI TGC'GCA
Cuts at: 2718 2718
Size: 4937
HaeII r_GCGC'y
Cuts at: 552 611 752 1467 2371 2619 3641 4620 552
Size: 59 141 715 904 248 1022 979 869
Fragments arranged by size:
1022 979 904 869 715 248 141 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 797 829 1240 1272 1820 1852 2779 2809 2811
Size: 32 411 32 548 32 927 30 2
Cuts at: 2811 2841 797
Size: 30 2893
Fragments arranged by size:
2893 927 548 411 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1976 1976
Size: 4937
KasI G'GCGC_C
Cuts at: 607 1463 2367 2615 607
Size: 856 904 248 2929
Fragments arranged by size:
2929 904 856 248
KpnI G_GTAC'C
Cuts at: 1957 1957
Size: 4937
MluI A'CGCG_T
Cuts at: 4370 4370
Size: 4937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 948 3608 3792 948
Size: 2660 184 2093
Fragments arranged by size:
2660 2093 184
MscI TGG'CCA
Cuts at: 1357 1910 2698 1357
Size: 553 788 3596
Fragments arranged by size:
3596 788 553
MslI CAynn'nnrTG
Cuts at: 785 1555 1660 1720 1859 2926 3053 785
Size: 770 105 60 139 1067 127 2669
Fragments arranged by size:
2669 1067 770 139 127 105 60
MspA1I CmG'CkG
Cuts at: 850 2048 2362 2722 3735 3980 850
Size: 1198 314 360 1013 245 1807
Fragments arranged by size:
1807 1198 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3118 3118
Size: 4937
NarI GG'CG_CC
Cuts at: 608 1464 2368 2616 608
Size: 856 904 248 2929
Fragments arranged by size:
2929 904 856 248
NcoI C'CATG_G
Cuts at: 1037 1491 1037
Size: 454 4483
NdeI CA'TA_TG
Cuts at: 1322 1322
Size: 4937
NgoMIV G'CCGG_C
Cuts at: 3116 3116
Size: 4937
NspI r_CATG'y
Cuts at: 691 1990 2490 3397 691
Size: 1299 500 907 2231
Fragments arranged by size:
2231 1299 907 500
PasI CC'CwG_GG
Cuts at: 140 140
Size: 4937
PciI A'CATG_T
Cuts at: 3393 3393
Size: 4937
PflMI CCAn_nnn'nTGG
Cuts at: 386 653 1431 1509 386
Size: 267 778 78 3814
Fragments arranged by size:
3814 778 267 78
PmeI GTTT'AAAC
Cuts at: 1936 1936
Size: 4937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4905
PpuMI rG'GwC_Cy
Cuts at: 681 979 1025 1217 681
Size: 298 46 192 4401
Fragments arranged by size:
4401 298 192 46
PspOMI G'GGCC_C
Cuts at: 201 985 201
Size: 784 4153
PstI C_TGCA'G
Cuts at: 338 451 1088 1984 2669 338
Size: 113 637 896 685 2606
Fragments arranged by size:
2606 896 685 637 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4937
PvuII CAG'CTG
Cuts at: 850 2362 2722 850
Size: 1512 360 3065
Fragments arranged by size:
3065 1512 360
RsrII CG'GwC_CG
Cuts at: 3132 3132
Size: 4937
SacI G_AGCT'C
Cuts at: 815 1951 815
Size: 1136 3801
SalI G'TCGA_C
Cuts at: 1974 1974
Size: 4937
SanDI GG'GwC_CC
Cuts at: 1025 1217 1025
Size: 192 4745
SapI GCTCTTCn'nnn_
Cuts at: 2960 3170 2960
Size: 210 4727
SbfI CC_TGCA'GG
Cuts at: 1088 1984 1088
Size: 896 4041
ScaI AGT'ACT
Cuts at: 4454 4454
Size: 4937
SexAI A'CCwGG_T
Cuts at: 1093 1093
Size: 4937
SfcI C'TryA_G
Cuts at: 33 334 447 1047 1084 1980 2665 3658 3849
Size: 301 113 600 37 896 685 993 191
Cuts at: 3849 33
Size: 1121
Fragments arranged by size:
1121 993 896 685 600 301 191 113
37
SfiI GGCCn_nnn'nGGCC
Cuts at: 1042 1042
Size: 4937
SfoI GGC'GCC
Cuts at: 609 1465 2369 2617 609
Size: 856 904 248 2929
Fragments arranged by size:
2929 904 856 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
SgrAI Cr'CCGG_yG
Cuts at: 1394 1394
Size: 4937
SmaI CCC'GGG
Cuts at: 487 1959 487
Size: 1472 3465
SmlI C'TyrA_G
Cuts at: 441 731 2092 3007 3499 3761 441
Size: 290 1361 915 492 262 1617
Fragments arranged by size:
1617 1361 915 492 290 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4937
SphI G_CATG'C
Cuts at: 691 1990 2490 691
Size: 1299 500 3138
Fragments arranged by size:
3138 1299 500
StyI C'CwwG_G
Cuts at: 1037 1454 1491 1553 2071 2425 1037
Size: 417 37 62 518 354 3549
Fragments arranged by size:
3549 518 417 354 62 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 472 760 1207 1464 1830 2520 2653 3112 3321
Size: 288 447 257 366 690 133 459 209
Cuts at: 3321 472
Size: 2088
Fragments arranged by size:
2088 690 459 447 366 288 257 209
133
TatI w'GTAC_w
Cuts at: 1328 4452 1328
Size: 3124 1813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 381 413 4454 4486 381
Size: 32 4041 32 832
Fragments arranged by size:
4041 832 32 32
Tth111I GACn'n_nGTC
Cuts at: 2734 2734
Size: 4937
XbaI T'CTAG_A
Cuts at: 1968 1968
Size: 4937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 553 2432 553
Size: 1879 3058
XhoI C'TCGA_G
Cuts at: 731 731
Size: 4937
XmaI C'CCGG_G
Cuts at: 485 1957 485
Size: 1472 3465
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SnaBI
SrfI SspI StuI SwaI XmnI ZraI