Restriction map for
pF1KE2560
(Circular) (Six-base) MAPSORT of: pF1KE2560.seq Check: 6361 from: 1 to: 3740
pF1KE2560 3740 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 707 778 707
Size: 71 3669
Acc65I G'GTAC_C
Cuts at: 756 756
Size: 3740
AclI AA'CG_TT
Cuts at: 372 3580 372
Size: 3208 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 743 1565 1997 2744 3012 635
Size: 108 822 432 747 268 1363
Fragments arranged by size:
1363 822 747 432 268 108
AflIII A'CryG_T
Cuts at: 300 459 1092 2196 3173 300
Size: 159 633 1104 977 867
Fragments arranged by size:
1104 977 867 633 159
AgeI A'CCGG_T
Cuts at: 2128 2128
Size: 3740
AlwNI CAG_nnn'CTG
Cuts at: 1045 2612 1045
Size: 1567 2173
ApaLI G'TGCA_C
Cuts at: 2510 2510
Size: 3740
ApoI r'AATT_y
Cuts at: 384 744 3093 3349 384
Size: 360 2349 256 775
Fragments arranged by size:
2349 775 360 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3740
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
AvaI C'yCGr_G
Cuts at: 177 202 760 177
Size: 25 558 3157
Fragments arranged by size:
3157 558 25
BamHI G'GATC_C
Cuts at: 765 765
Size: 3740
BanI G'GyrC_C
Cuts at: 156 756 1170 1418 1453 156
Size: 600 414 248 35 2443
Fragments arranged by size:
2443 600 414 248 35
BanII G_rGCy'C
Cuts at: 754 1784 754
Size: 1030 2710
BbeI G_GCGC'C
Cuts at: 160 1174 1422 160
Size: 1014 248 2478
Fragments arranged by size:
2478 1014 248
BciVI GTATCCnnnnn_n'
Cuts at: 1638 2399 3740 1638
Size: 761 1341 1638
Fragments arranged by size:
1638 1341 761
BclI T'GATC_A
Cuts at: 1260 1260
Size: 3740
BglII A'GATC_T
Cuts at: 282 1255 3292 282
Size: 973 2037 730
Fragments arranged by size:
2037 973 730
BlpI GC'TnA_GC
Cuts at: 852 852
Size: 3740
Bme1580I G_kGCm'C
Cuts at: 1365 1458 2514 1365
Size: 93 1056 2591
Fragments arranged by size:
2591 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 952 1033 1368 3512 952
Size: 81 335 2144 1180
Fragments arranged by size:
2144 1180 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 134 495 2107 134
Size: 361 1612 1767
Fragments arranged by size:
1767 1612 361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1795 2287 2585 916
Size: 879 492 298 2071
Fragments arranged by size:
2071 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3444 3444
Size: 3740
BsaAI yAC'GTr
Cuts at: 462 1093 462
Size: 631 3109
BsaBI GATnn'nnATC
Cuts at: 6 1254 3291 6
Size: 1248 2037 455
Fragments arranged by size:
2037 1248 455
BsaHI Gr'CG_yC
Cuts at: 157 1171 1419 2121 3617 157
Size: 1014 248 702 1496 280
Fragments arranged by size:
1496 1014 702 280 248
BsaWI w'CCGG_w
Cuts at: 168 929 1450 2128 2402 2549 2960 168
Size: 761 521 678 274 147 411 948
Fragments arranged by size:
948 761 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1165 1923 2500 2996 1165
Size: 758 577 496 1909
Fragments arranged by size:
1909 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1282 1328 2536 104
Size: 1178 46 1208 1308
Fragments arranged by size:
1308 1208 1178 46
BsiHKAI G_wGCw'C
Cuts at: 754 1532 1722 2514 754
Size: 778 190 792 1980
Fragments arranged by size:
1980 792 778 190
BsmI GAATG_Cn'
Cuts at: 264 264
Size: 3740
Bsp1286I G_dGCh'C
Cuts at: 754 1365 1458 1532 1722 1784 2514 754
Size: 611 93 74 190 62 730 1980
Fragments arranged by size:
1980 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 168 2960 168
Size: 2792 948
BspHI T'CATG_A
Cuts at: 2916 2916
Size: 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 790 1004 1306 1687 790
Size: 214 302 381 2843
Fragments arranged by size:
2843 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2032 2086 3570 3735 50
Size: 1982 54 1484 165 55
Fragments arranged by size:
1982 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1652 1652
Size: 3740
BsrFI r'CCGG_y
Cuts at: 1738 1919 2128 1738
Size: 181 209 3350
Fragments arranged by size:
3350 209 181
BssSI C'ACGA_G
Cuts at: 2011 2369 2011
Size: 358 3382
BstAPI GCAn_nnn'nTGC
Cuts at: 1117 1117
Size: 3740
BstBI TT'CG_AA
Cuts at: 2101 2101
Size: 3740
BstYI r'GATC_y
Cuts at: 1 282 765 1232 1255 1590 1836 2848 2934
Size: 281 483 467 23 335 246 1012 86
Cuts at: 2934 2946 3292 1
Size: 12 346 449
Fragments arranged by size:
1012 483 467 449 346 335 281 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1690 1871 2034 1690
Size: 181 163 3396
Fragments arranged by size:
3396 181 163
BtsI GCAGTG_nn'
Cuts at: 983 983
Size: 3740
DraI TTT'AAA
Cuts at: 345 668 739 345
Size: 323 71 3346
Fragments arranged by size:
3346 323 71
DrdI GACnn_nn'nnGTC
Cuts at: 1446 2304 1446
Size: 858 2882
EaeI y'GGCC_r
Cuts at: 1325 1499 1890 1917 3262 1325
Size: 174 391 27 1345 1803
Fragments arranged by size:
1803 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1325 1325
Size: 3740
EarI CTCTTCn'nnn_
Cuts at: 677 1763 1973 677
Size: 1086 210 2444
Fragments arranged by size:
2444 1086 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1948 2258 2404 3550 1948
Size: 310 146 1146 2138
Fragments arranged by size:
2138 1146 310 146
EcoICRI GAG'CTC
Cuts at: 752 752
Size: 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 743 1565 1997 2744 3012 635
Size: 108 822 432 747 268 1363
Fragments arranged by size:
1363 822 747 432 268 108
EcoO109I rG'GnC_Cy
Cuts at: 879 879
Size: 3740
EcoRI G'AATT_C
Cuts at: 744 744
Size: 3740
EcoRV GAT'ATC
Cuts at: 621 621
Size: 3740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1759 1759
Size: 3740
FspI TGC'GCA
Cuts at: 1521 1521
Size: 3740
HaeII r_GCGC'y
Cuts at: 160 1174 1422 2444 3423 160
Size: 1014 248 1022 979 477
Fragments arranged by size:
1022 1014 979 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 226 258 1582 1612 1614 1644 226
Size: 32 1324 30 2 30 2322
Fragments arranged by size:
2322 1324 32 30 30 2
HincII GTy'rAC
Cuts at: 779 779
Size: 3740
HindIII A'AGCT_T
Cuts at: 208 208
Size: 3740
KasI G'GCGC_C
Cuts at: 156 1170 1418 156
Size: 1014 248 2478
Fragments arranged by size:
2478 1014 248
KpnI G_GTAC'C
Cuts at: 760 760
Size: 3740
MluI A'CGCG_T
Cuts at: 3173 3173
Size: 3740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2595 2411
Size: 184 3556
MscI TGG'CCA
Cuts at: 1501 1501
Size: 3740
MslI CAynn'nnrTG
Cuts at: 1729 1856 1729
Size: 127 3613
MspA1I CmG'CkG
Cuts at: 851 1165 1525 2538 2783 851
Size: 314 360 1013 245 1808
Fragments arranged by size:
1808 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1921 1921
Size: 3740
NarI GG'CG_CC
Cuts at: 157 1171 1419 157
Size: 1014 248 2478
Fragments arranged by size:
2478 1014 248
NgoMIV G'CCGG_C
Cuts at: 1919 1919
Size: 3740
NspI r_CATG'y
Cuts at: 304 793 1293 2200 304
Size: 489 500 907 1844
Fragments arranged by size:
1844 907 500 489
PciI A'CATG_T
Cuts at: 300 2196 300
Size: 1896 1844
PmeI GTTT'AAAC
Cuts at: 739 739
Size: 3740
PmlI CAC'GTG
Cuts at: 462 462
Size: 3740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3708
PsiI TTA'TAA
Cuts at: 517 608 517
Size: 91 3649
PstI C_TGCA'G
Cuts at: 787 1472 787
Size: 685 3055
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3740
PvuII CAG'CTG
Cuts at: 1165 1525 1165
Size: 360 3380
RsrII CG'GwC_CG
Cuts at: 1935 1935
Size: 3740
SacI G_AGCT'C
Cuts at: 754 754
Size: 3740
SalI G'TCGA_C
Cuts at: 777 777
Size: 3740
SapI GCTCTTCn'nnn_
Cuts at: 1763 1973 1763
Size: 210 3530
SbfI CC_TGCA'GG
Cuts at: 787 787
Size: 3740
ScaI AGT'ACT
Cuts at: 366 3257 366
Size: 2891 849
SfcI C'TryA_G
Cuts at: 33 286 783 1468 2461 2652 33
Size: 253 497 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 253 191
SfoI GGC'GCC
Cuts at: 158 1172 1420 158
Size: 1014 248 2478
Fragments arranged by size:
2478 1014 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
SmaI CCC'GGG
Cuts at: 179 762 179
Size: 583 3157
SmlI C'TyrA_G
Cuts at: 202 895 1810 2302 2564 202
Size: 693 915 492 262 1378
Fragments arranged by size:
1378 915 693 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3740
SphI G_CATG'C
Cuts at: 793 1293 793
Size: 500 3240
StyI C'CwwG_G
Cuts at: 874 1228 874
Size: 354 3386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1323 1456 1915 2124 1323
Size: 133 459 209 2939
Fragments arranged by size:
2939 459 209 133
TatI w'GTAC_w
Cuts at: 364 425 3255 364
Size: 61 2830 849
Fragments arranged by size:
2830 849 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 3708
Tth111I GACn'n_nGTC
Cuts at: 1537 1537
Size: 3740
XbaI T'CTAG_A
Cuts at: 771 771
Size: 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1235 1235
Size: 3740
XhoI C'TCGA_G
Cuts at: 202 202
Size: 3740
XmaI C'CCGG_G
Cuts at: 177 760 177
Size: 583 3157
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PmlI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI