Restriction map for
pF1KE2555
(Circular) (Six-base) MAPSORT of: pF1KE2555.seq Check: 3075 from: 1 to: 4481
pF1KE2555 4481 bp
With 182 enzymes: SgfI *
December 15, 2014 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1359 1359
Size: 4481
AccI GT'mk_AC
Cuts at: 1519 1519
Size: 4481
Acc65I G'GTAC_C
Cuts at: 1497 1497
Size: 4481
AclI AA'CG_TT
Cuts at: 4321 4321
Size: 4481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 775 935 2306 2738 3485 3753 510
Size: 265 160 1371 432 747 268 1238
Fragments arranged by size:
1371 1238 747 432 268 265 160
AfeI AGC'GCT
Cuts at: 452 989 452
Size: 537 3944
AflIII A'CryG_T
Cuts at: 1833 2937 3914 1833
Size: 1104 977 2400
Fragments arranged by size:
2400 1104 977
AgeI A'CCGG_T
Cuts at: 2869 2869
Size: 4481
AlwNI CAG_nnn'CTG
Cuts at: 612 810 1374 1786 3353 612
Size: 198 564 412 1567 1740
Fragments arranged by size:
1740 1567 564 412 198
ApaLI G'TGCA_C
Cuts at: 3251 3251
Size: 4481
ApoI r'AATT_y
Cuts at: 1485 3834 4090 1485
Size: 2349 256 1876
Fragments arranged by size:
2349 1876 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
AvaI C'yCGr_G
Cuts at: 289 1501 289
Size: 1212 3269
BamHI G'GATC_C
Cuts at: 263 1506 263
Size: 1243 3238
BanI G'GyrC_C
Cuts at: 178 565 1359 1399 1497 1911 2159 2194 178
Size: 387 794 40 98 414 248 35 2465
Fragments arranged by size:
2465 794 414 387 248 98 40 35
BanII G_rGCy'C
Cuts at: 475 830 932 1495 2525 475
Size: 355 102 563 1030 2431
Fragments arranged by size:
2431 1030 563 355 102
BbeI G_GCGC'C
Cuts at: 1363 1403 1915 2163 1363
Size: 40 512 248 3681
Fragments arranged by size:
3681 512 248 40
BbsI GAAGACnn'nnnn_
Cuts at: 948 957 999 1020 948
Size: 9 42 21 4409
Fragments arranged by size:
4409 42 21 9
BbvCI CC'TCA_GC
Cuts at: 598 598
Size: 4481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 534 568 661 695 534
Size: 34 93 34 4320
Fragments arranged by size:
4320 93 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2379 3140 4481 2379
Size: 761 1341 2379
Fragments arranged by size:
2379 1341 761
BclI T'GATC_A
Cuts at: 2001 2001
Size: 4481
BglII A'GATC_T
Cuts at: 974 1996 4033 974
Size: 1022 2037 1422
Fragments arranged by size:
2037 1422 1022
BlpI GC'TnA_GC
Cuts at: 824 1593 824
Size: 769 3712
Bme1580I G_kGCm'C
Cuts at: 181 568 1226 1250 2106 2199 3255 181
Size: 387 658 24 856 93 1056 1407
Fragments arranged by size:
1407 1056 856 658 387 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 851 1693 1774 2109 4253 851
Size: 842 81 335 2144 1079
Fragments arranged by size:
2144 1079 842 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 764 1465 2848 764
Size: 701 1383 2397
Fragments arranged by size:
2397 1383 701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 832 919 817
Size: 15 87 4379
Fragments arranged by size:
4379 87 15
Bpu10I CC'TnA_GC
Cuts at: 268 598 739 803 268
Size: 330 141 64 3946
Fragments arranged by size:
3946 330 141 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 2536 3028 3326 1657
Size: 879 492 298 2812
Fragments arranged by size:
2812 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 667 808 4185 667
Size: 141 3377 963
Fragments arranged by size:
3377 963 141
BsaAI yAC'GTr
Cuts at: 1245 1834 1245
Size: 589 3892
BsaBI GATnn'nnATC
Cuts at: 6 1995 4032 6
Size: 1989 2037 455
Fragments arranged by size:
2037 1989 455
BsaHI Gr'CG_yC
Cuts at: 122 668 1360 1400 1912 2160 2862 4358 122
Size: 546 692 40 512 248 702 1496 245
Fragments arranged by size:
1496 702 692 546 512 248 245 40
BsaWI w'CCGG_w
Cuts at: 1670 2191 2869 3143 3290 3701 1670
Size: 521 678 274 147 411 2450
Fragments arranged by size:
2450 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 131 349 820 1102 131
Size: 218 471 282 3510
Fragments arranged by size:
3510 471 282 218
BseYI C'CCAG_C
Cuts at: 174 332 561 616 1134 1906 2664 3241 3737
Size: 158 229 55 518 772 758 577 496
Cuts at: 3737 174
Size: 918
Fragments arranged by size:
918 772 758 577 518 496 229 158
55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 551
Size: 4481
BsiEI CG_ry'CG
Cuts at: 104 2023 2069 3277 104
Size: 1919 46 1208 1308
Fragments arranged by size:
1919 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 274 830 1495 2273 2463 3255 274
Size: 556 665 778 190 792 1500
Fragments arranged by size:
1500 792 778 665 556 190
BsmI GAATG_Cn'
Cuts at: 401 401
Size: 4481
BsmBI CGTCTCn'nnnn_
Cuts at: 113 385 113
Size: 272 4209
Bsp1286I G_dGCh'C
Cuts at: 181 274 475 568 830 932 1226 1250 1495
Size: 93 201 93 262 102 294 24 245
Cuts at: 1495 2106 2199 2273 2463 2525 3255 181
Size: 611 93 74 190 62 730 1407
Fragments arranged by size:
1407 730 611 294 262 245 201 190
102 93 93 93 74 62 24
BspEI T'CCGG_A
Cuts at: 3701 3701
Size: 4481
BspHI T'CATG_A
Cuts at: 1406 3657 1406
Size: 2251 2230
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1359 1531 1745 2047 2428 1359
Size: 172 214 302 381 3412
Fragments arranged by size:
3412 381 302 214 172
BsrBI CCG'CTC
Cuts at: 50 2773 2827 4311 4476 50
Size: 2723 54 1484 165 55
Fragments arranged by size:
2723 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2393 2393
Size: 4481
BsrFI r'CCGG_y
Cuts at: 244 1181 1396 2479 2660 2869 244
Size: 937 215 1083 181 209 1856
Fragments arranged by size:
1856 1083 937 215 209 181
BssSI C'ACGA_G
Cuts at: 1381 2752 3110 1381
Size: 1371 358 2752
Fragments arranged by size:
2752 1371 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1374 1858 1374
Size: 484 3997
BstBI TT'CG_AA
Cuts at: 2842 2842
Size: 4481
BstEII G'GTnAC_C
Cuts at: 1050 1050
Size: 4481
BstXI CCAn_nnnn'nTGG
Cuts at: 498 663 1356 498
Size: 165 693 3623
Fragments arranged by size:
3623 693 165
BstYI r'GATC_y
Cuts at: 1 263 620 888 974 1506 1973 1996 2331
Size: 262 357 268 86 532 467 23 335
Cuts at: 2331 2577 3589 3675 3687 4033 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 532 467 449 357 346 335 268
262 246 86 86 23 12
BtgI C'CryG_G
Cuts at: 107 127 497 650 1046 107
Size: 20 370 153 396 3542
Fragments arranged by size:
3542 396 370 153 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2431 2612 2775 2431
Size: 181 163 4137
Fragments arranged by size:
4137 181 163
BtsI GCAGTG_nn'
Cuts at: 1724 1724
Size: 4481
DraI TTT'AAA
Cuts at: 1480 1480
Size: 4481
DraIII CAC_nnn'GTG
Cuts at: 1352 1352
Size: 4481
DrdI GACnn_nn'nnGTC
Cuts at: 327 2187 3045 327
Size: 1860 858 1763
Fragments arranged by size:
1860 1763 858
EaeI y'GGCC_r
Cuts at: 236 419 603 653 776 1165 1394 2066 2240
Size: 183 184 50 123 389 229 672 174
Cuts at: 2240 2631 2658 4003 236
Size: 391 27 1345 714
Fragments arranged by size:
1345 714 672 391 389 229 184 183
174 123 50 27
EagI C'GGCC_G
Cuts at: 2066 2066
Size: 4481
EarI CTCTTCn'nnn_
Cuts at: 355 556 598 1120 1234 1279 1465 2504 2714
Size: 201 42 522 114 45 186 1039 210
Cuts at: 2714 355
Size: 2122
Fragments arranged by size:
2122 1039 522 210 201 186 114 45
42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2689 2999 3145 4291 2689
Size: 310 146 1146 2879
Fragments arranged by size:
2879 1146 310 146
EcoICRI GAG'CTC
Cuts at: 828 1493 828
Size: 665 3816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 775 817 832 919 935 2306 2738 3485
Size: 265 42 15 87 16 1371 432 747
Cuts at: 3485 3753 510
Size: 268 1238
Fragments arranged by size:
1371 1238 747 432 268 265 87 42
16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 901 1462 901
Size: 561 3920
EcoO109I rG'GnC_Cy
Cuts at: 286 609 1256 1620 286
Size: 323 647 364 3147
Fragments arranged by size:
3147 647 364 323
EcoRI G'AATT_C
Cuts at: 1485 1485
Size: 4481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4481
FspI TGC'GCA
Cuts at: 400 2262 400
Size: 1862 2619
FspAI rTGC'GCAy
Cuts at: 400 400
Size: 4481
HaeII r_GCGC'y
Cuts at: 454 991 1363 1403 1471 1915 2163 3185 4164
Size: 537 372 40 68 444 248 1022 979
Cuts at: 4164 454
Size: 771
Fragments arranged by size:
1022 979 771 537 444 372 248 68
40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 898 905 930 2323 2353 2355 2385 873
Size: 25 7 25 1393 30 2 30 2969
Fragments arranged by size:
2969 1393 30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 1520 1520
Size: 4481
KasI G'GCGC_C
Cuts at: 1359 1399 1911 2159 1359
Size: 40 512 248 3681
Fragments arranged by size:
3681 512 248 40
KpnI G_GTAC'C
Cuts at: 1501 1501
Size: 4481
MfeI C'AATT_G
Cuts at: 312 312
Size: 4481
MluI A'CGCG_T
Cuts at: 3914 3914
Size: 4481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3152 3336 3152
Size: 184 4297
MscI TGG'CCA
Cuts at: 421 655 778 1167 2242 421
Size: 234 123 389 1075 2660
Fragments arranged by size:
2660 1075 389 234 123
MslI CAynn'nnrTG
Cuts at: 496 718 1045 1303 2470 2597 496
Size: 222 327 258 1167 127 2380
Fragments arranged by size:
2380 1167 327 258 222 127
MspA1I CmG'CkG
Cuts at: 129 336 561 602 1592 1906 2266 3279 3524
Size: 207 225 41 990 314 360 1013 245
Cuts at: 3524 129
Size: 1086
Fragments arranged by size:
1086 1013 990 360 314 245 225 207
41
NaeI GCC'GGC
Cuts at: 246 1183 1398 2662 246
Size: 937 215 1264 2065
Fragments arranged by size:
2065 1264 937 215
NarI GG'CG_CC
Cuts at: 1360 1400 1912 2160 1360
Size: 40 512 248 3681
Fragments arranged by size:
3681 512 248 40
NcoI C'CATG_G
Cuts at: 107 497 650 1046 107
Size: 390 153 396 3542
Fragments arranged by size:
3542 396 390 153
NgoMIV G'CCGG_C
Cuts at: 244 1181 1396 2660 244
Size: 937 215 1264 2065
Fragments arranged by size:
2065 1264 937 215
NspI r_CATG'y
Cuts at: 1534 2034 2941 1534
Size: 500 907 3074
Fragments arranged by size:
3074 907 500
PasI CC'CwG_GG
Cuts at: 684 1425 684
Size: 741 3740
PciI A'CATG_T
Cuts at: 2937 2937
Size: 4481
PflMI CCAn_nnn'nTGG
Cuts at: 612 612
Size: 4481
PfoI T'CCnGG_A
Cuts at: 386 386
Size: 4481
PmeI GTTT'AAAC
Cuts at: 1480 1480
Size: 4481
PmlI CAC'GTG
Cuts at: 1245 1245
Size: 4481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4449
PpuMI rG'GwC_Cy
Cuts at: 286 1256 286
Size: 970 3511
PstI C_TGCA'G
Cuts at: 1528 2213 1528
Size: 685 3796
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4481
PvuII CAG'CTG
Cuts at: 336 561 1906 2266 336
Size: 225 1345 360 2551
Fragments arranged by size:
2551 1345 360 225
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 4481
SacI G_AGCT'C
Cuts at: 830 1495 830
Size: 665 3816
SacII CC_GC'GG
Cuts at: 130 130
Size: 4481
SalI G'TCGA_C
Cuts at: 1518 1518
Size: 4481
SanDI GG'GwC_CC
Cuts at: 286 286
Size: 4481
SapI GCTCTTCn'nnn_
Cuts at: 355 2504 2714 355
Size: 2149 210 2122
Fragments arranged by size:
2149 2122 210
SbfI CC_TGCA'GG
Cuts at: 1528 1528
Size: 4481
ScaI AGT'ACT
Cuts at: 3998 3998
Size: 4481
SexAI A'CCwGG_T
Cuts at: 143 1334 143
Size: 1191 3290
SfcI C'TryA_G
Cuts at: 33 538 582 1524 2209 3202 3393 33
Size: 505 44 942 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 505 191 44
SfoI GGC'GCC
Cuts at: 1361 1401 1913 2161 1361
Size: 40 512 248 3681
Fragments arranged by size:
3681 512 248 40
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
SmaI CCC'GGG
Cuts at: 1503 1503
Size: 4481
SmlI C'TyrA_G
Cuts at: 1636 2551 3043 3305 1636
Size: 915 492 262 2812
Fragments arranged by size:
2812 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4481
SphI G_CATG'C
Cuts at: 1534 2034 1534
Size: 500 3981
StyI C'CwwG_G
Cuts at: 107 497 650 1046 1615 1969 107
Size: 390 153 396 569 354 2619
Fragments arranged by size:
2619 569 396 390 354 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 306 2064 2197 2656 2865 306
Size: 1758 133 459 209 1922
Fragments arranged by size:
1922 1758 459 209 133
TatI w'GTAC_w
Cuts at: 3996 3996
Size: 4481
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 171 203 3998 4030 171
Size: 32 3795 32 622
Fragments arranged by size:
3795 622 32 32
Tth111I GACn'n_nGTC
Cuts at: 2278 2278
Size: 4481
XbaI T'CTAG_A
Cuts at: 1512 1512
Size: 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 613 1044 1976 613
Size: 431 932 3118
Fragments arranged by size:
3118 932 431
XmaI C'CCGG_G
Cuts at: 1501 1501
Size: 4481
XmnI GAAnn'nnTTC
Cuts at: 199 948 199
Size: 749 3732
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI