Restriction map for
pF1KE2554
(Circular) (Six-base) MAPSORT of: pF1KE2554.seq Check: 48 from: 1 to: 4196
pF1KE2554 4196 bp
With 182 enzymes: SgfI *
December 15, 2014 12:34 ..
AccI GT'mk_AC
Cuts at: 1234 1234
Size: 4196
Acc65I G'GTAC_C
Cuts at: 1212 1212
Size: 4196
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 900 1062 2021 2453 3200 3468 652
Size: 248 162 959 432 747 268 1380
Fragments arranged by size:
1380 959 747 432 268 248 162
AflIII A'CryG_T
Cuts at: 1548 2652 3629 1548
Size: 1104 977 2115
Fragments arranged by size:
2115 1104 977
AgeI A'CCGG_T
Cuts at: 2584 2584
Size: 4196
AleI CACnn'nnGTG
Cuts at: 365 865 365
Size: 500 3696
AlwNI CAG_nnn'CTG
Cuts at: 1501 3068 1501
Size: 1567 2629
ApaLI G'TGCA_C
Cuts at: 835 858 964 2966 835
Size: 23 106 2002 2065
Fragments arranged by size:
2065 2002 106 23
ApoI r'AATT_y
Cuts at: 1105 1200 3549 3805 1105
Size: 95 2349 256 1496
Fragments arranged by size:
2349 1496 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 1216 1216
Size: 4196
BamHI G'GATC_C
Cuts at: 1221 1221
Size: 4196
BanI G'GyrC_C
Cuts at: 732 1212 1626 1874 1909 732
Size: 480 414 248 35 3019
Fragments arranged by size:
3019 480 414 248 35
BanII G_rGCy'C
Cuts at: 186 194 253 887 1210 2240 186
Size: 8 59 634 323 1030 2142
Fragments arranged by size:
2142 1030 634 323 59 8
BbeI G_GCGC'C
Cuts at: 736 1630 1878 736
Size: 894 248 3054
Fragments arranged by size:
3054 894 248
BbsI GAAGACnn'nnnn_
Cuts at: 834 834
Size: 4196
BciVI GTATCCnnnnn_n'
Cuts at: 2094 2855 4196 2094
Size: 761 1341 2094
Fragments arranged by size:
2094 1341 761
BclI T'GATC_A
Cuts at: 1716 1716
Size: 4196
BfrBI ATG'CAT
Cuts at: 376 376
Size: 4196
BglI GCCn_nnn'nGGC
Cuts at: 988 988
Size: 4196
BglII A'GATC_T
Cuts at: 1711 3748 1711
Size: 2037 2159
BlpI GC'TnA_GC
Cuts at: 1308 1308
Size: 4196
Bme1580I G_kGCm'C
Cuts at: 839 862 968 1821 1914 2970 839
Size: 23 106 853 93 1056 2065
Fragments arranged by size:
2065 1056 853 106 93 23
BmgBI CAC'GTC
Cuts at: 840 840
Size: 4196
BmrI ACTGGGnnnn_n'
Cuts at: 523 1408 1489 1824 3968 523
Size: 885 81 335 2144 751
Fragments arranged by size:
2144 885 751 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 4196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1372 2251 2743 3041 937
Size: 435 879 492 298 2092
Fragments arranged by size:
2092 879 492 435 298
BsaI GGTCTCn'nnnn_
Cuts at: 865 3900 865
Size: 3035 1161
BsaAI yAC'GTr
Cuts at: 1549 1549
Size: 4196
BsaBI GATnn'nnATC
Cuts at: 6 1710 3747 6
Size: 1704 2037 455
Fragments arranged by size:
2037 1704 455
BsaHI Gr'CG_yC
Cuts at: 733 1627 1875 2577 4073 733
Size: 894 248 702 1496 856
Fragments arranged by size:
1496 894 856 702 248
BsaWI w'CCGG_w
Cuts at: 1385 1906 2584 2858 3005 3416 1385
Size: 521 678 274 147 411 2165
Fragments arranged by size:
2165 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1175 1205 1175
Size: 30 4166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 166 243 163
Size: 3 77 4116
Fragments arranged by size:
4116 77 3
BseYI C'CCAG_C
Cuts at: 910 1621 2379 2956 3452 910
Size: 711 758 577 496 1654
Fragments arranged by size:
1654 758 711 577 496
BsiEI CG_ry'CG
Cuts at: 104 1738 1784 2992 104
Size: 1634 46 1208 1308
Fragments arranged by size:
1634 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 194 253 839 862 887 968 1210 1988 2178
Size: 59 586 23 25 81 242 778 190
Cuts at: 2178 2970 194
Size: 792 1420
Fragments arranged by size:
1420 792 778 586 242 190 81 59
25 23
BsiWI C'GTAC_G
Cuts at: 406 406
Size: 4196
Bsp1286I G_dGCh'C
Cuts at: 186 194 253 839 862 887 968 1210 1821
Size: 8 59 586 23 25 81 242 611
Cuts at: 1821 1914 1988 2178 2240 2970 186
Size: 93 74 190 62 730 1412
Fragments arranged by size:
1412 730 611 586 242 190 93 81
74 62 59 25 23 8
BspEI T'CCGG_A
Cuts at: 3416 3416
Size: 4196
BspHI T'CATG_A
Cuts at: 3372 3372
Size: 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1246 1460 1762 2143 1246
Size: 214 302 381 3299
Fragments arranged by size:
3299 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2488 2542 4026 4191 50
Size: 2438 54 1484 165 55
Fragments arranged by size:
2438 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1160 2108 1160
Size: 948 3248
BsrFI r'CCGG_y
Cuts at: 712 2194 2375 2584 712
Size: 1482 181 209 2324
Fragments arranged by size:
2324 1482 209 181
BssSI C'ACGA_G
Cuts at: 967 2467 2825 967
Size: 1500 358 2338
Fragments arranged by size:
2338 1500 358
BstAPI GCAn_nnn'nTGC
Cuts at: 479 1573 479
Size: 1094 3102
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstEII G'GTnAC_C
Cuts at: 942 942
Size: 4196
BstYI r'GATC_y
Cuts at: 1 1221 1688 1711 2046 2292 3304 3390 3402
Size: 1220 467 23 335 246 1012 86 12
Cuts at: 3402 3748 1
Size: 346 449
Fragments arranged by size:
1220 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 134 134
Size: 4196
BtgI C'CryG_G
Cuts at: 107 107
Size: 4196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 265 1038 1439 265
Size: 773 401 3022
Fragments arranged by size:
3022 773 401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1014 1049 1014
Size: 35 4161
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DrdI GACnn_nn'nnGTC
Cuts at: 1902 2760 1902
Size: 858 3338
EaeI y'GGCC_r
Cuts at: 623 1166 1781 1955 2346 2373 3718 623
Size: 543 615 174 391 27 1345 1101
Fragments arranged by size:
1345 1101 615 543 391 174 27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 140 559 931 2219 2429 140
Size: 419 372 1288 210 1907
Fragments arranged by size:
1907 1288 419 372 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2404 2714 2860 4006 2404
Size: 310 146 1146 2594
Fragments arranged by size:
2594 1146 310 146
EcoICRI GAG'CTC
Cuts at: 192 251 885 1208 192
Size: 59 634 323 3180
Fragments arranged by size:
3180 634 323 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 652 900 1062 2021 2453 3200 3468 445
Size: 207 248 162 959 432 747 268 1173
Fragments arranged by size:
1173 959 747 432 268 248 207 162
EcoO109I rG'GnC_Cy
Cuts at: 299 1335 299
Size: 1036 3160
EcoRI G'AATT_C
Cuts at: 1200 1200
Size: 4196
EcoRV GAT'ATC
Cuts at: 1086 1086
Size: 4196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 414 2215 414
Size: 1801 2395
FspI TGC'GCA
Cuts at: 1060 1977 1060
Size: 917 3279
HaeII r_GCGC'y
Cuts at: 736 1630 1878 2900 3879 736
Size: 894 248 1022 979 1053
Fragments arranged by size:
1053 1022 979 894 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 334 366 2038 2068 2070 2100 286
Size: 32 16 32 1672 30 2 30 2382
Fragments arranged by size:
2382 1672 32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 1002 1235 1002
Size: 233 3963
KasI G'GCGC_C
Cuts at: 732 1626 1874 732
Size: 894 248 3054
Fragments arranged by size:
3054 894 248
KpnI G_GTAC'C
Cuts at: 1216 1216
Size: 4196
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 526 2867 3051 526
Size: 2341 184 1671
Fragments arranged by size:
2341 1671 184
MscI TGG'CCA
Cuts at: 1957 1957
Size: 4196
MslI CAynn'nnrTG
Cuts at: 365 377 865 2185 2312 365
Size: 12 488 1320 127 2249
Fragments arranged by size:
2249 1320 488 127 12
MspA1I CmG'CkG
Cuts at: 449 851 1307 1621 1981 2994 3239 449
Size: 402 456 314 360 1013 245 1406
Fragments arranged by size:
1406 1013 456 402 360 314 245
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 733 1627 1875 733
Size: 894 248 3054
Fragments arranged by size:
3054 894 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4196
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NsiI A_TGCA'T
Cuts at: 378 378
Size: 4196
NspI r_CATG'y
Cuts at: 214 380 522 1249 1749 2656 214
Size: 166 142 727 500 907 1754
Fragments arranged by size:
1754 907 727 500 166 142
PciI A'CATG_T
Cuts at: 2652 2652
Size: 4196
PfoI T'CCnGG_A
Cuts at: 140 244 140
Size: 104 4092
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 4164
PstI C_TGCA'G
Cuts at: 284 1243 1928 284
Size: 959 685 2552
Fragments arranged by size:
2552 959 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 449 1621 1981 449
Size: 1172 360 2664
Fragments arranged by size:
2664 1172 360
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 194 253 887 1210 194
Size: 59 634 323 3180
Fragments arranged by size:
3180 634 323 59
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
SbfI CC_TGCA'GG
Cuts at: 1243 1243
Size: 4196
ScaI AGT'ACT
Cuts at: 487 3713 487
Size: 3226 970
SexAI A'CCwGG_T
Cuts at: 938 938
Size: 4196
SfcI C'TryA_G
Cuts at: 33 280 1239 1924 2917 3108 33
Size: 247 959 685 993 191 1121
Fragments arranged by size:
1121 993 959 685 247 191
SfoI GGC'GCC
Cuts at: 734 1628 1876 734
Size: 894 248 3054
Fragments arranged by size:
3054 894 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SmaI CCC'GGG
Cuts at: 1218 1218
Size: 4196
SmlI C'TyrA_G
Cuts at: 952 1351 2266 2758 3020 952
Size: 399 915 492 262 2128
Fragments arranged by size:
2128 915 492 399 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 214 1249 1749 214
Size: 1035 500 2661
Fragments arranged by size:
2661 1035 500
StuI AGG'CCT
Cuts at: 637 637
Size: 4196
StyI C'CwwG_G
Cuts at: 107 1330 1684 107
Size: 1223 354 2619
Fragments arranged by size:
2619 1223 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1779 1912 2371 2580 1779
Size: 133 459 209 3395
Fragments arranged by size:
3395 459 209 133
TatI w'GTAC_w
Cuts at: 485 608 3711 485
Size: 123 3103 970
Fragments arranged by size:
3103 970 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 4164
Tth111I GACn'n_nGTC
Cuts at: 1993 1993
Size: 4196
XbaI T'CTAG_A
Cuts at: 1227 1227
Size: 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1691 1691
Size: 4196
XmaI C'CCGG_G
Cuts at: 1216 1216
Size: 4196
XmnI GAAnn'nnTTC
Cuts at: 637 673 637
Size: 36 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmtI Bpu10I BsgI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI