Restriction map for
pF1KE2553
(Circular) (Six-base) MAPSORT of: pF1KE2553.seq Check: 3722 from: 1 to: 3887
pF1KE2553 3887 bp
With 182 enzymes: SgfI *
December 15, 2014 12:34 ..
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 1712 2144 2891 3159 200
Size: 1512 432 747 268 928
Fragments arranged by size:
1512 928 747 432 268
AflIII A'CryG_T
Cuts at: 458 1239 2343 3320 458
Size: 781 1104 977 1025
Fragments arranged by size:
1104 1025 977 781
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AleI CACnn'nnGTG
Cuts at: 461 461
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 1192 2759 1192
Size: 1567 2320
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 668 891 3240 3496 668
Size: 223 2349 256 1059
Fragments arranged by size:
2349 1059 256 223
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 148 782 907 148
Size: 634 125 3128
Fragments arranged by size:
3128 634 125
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 903 1317 1565 1600 903
Size: 414 248 35 3190
Fragments arranged by size:
3190 414 248 35
BanII G_rGCy'C
Cuts at: 172 340 901 1931 172
Size: 168 561 1030 2128
Fragments arranged by size:
2128 1030 561 168
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglII A'GATC_T
Cuts at: 640 711 1402 3439 640
Size: 71 691 2037 1088
Fragments arranged by size:
2037 1088 691 71
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 1512 1605 2661 1512
Size: 93 1056 2738
Fragments arranged by size:
2738 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 383 1099 1180 1515 3659 383
Size: 716 81 335 2144 611
Fragments arranged by size:
2144 716 611 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1063 1942 2434 2732 487
Size: 576 879 492 298 1642
Fragments arranged by size:
1642 879 576 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3591 3591
Size: 3887
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 1318 1566 2268 3764 1318
Size: 248 702 1496 1441
Fragments arranged by size:
1496 1441 702 248
BsaWI w'CCGG_w
Cuts at: 1076 1597 2275 2549 2696 3107 1076
Size: 521 678 274 147 411 1856
Fragments arranged by size:
1856 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 333
Size: 3887
BseYI C'CCAG_C
Cuts at: 1312 2070 2647 3143 1312
Size: 758 577 496 2056
Fragments arranged by size:
2056 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 528 1429 1475 2683 104
Size: 424 901 46 1208 1308
Fragments arranged by size:
1308 1208 901 424 46
BsiHKAI G_wGCw'C
Cuts at: 901 1679 1869 2661 901
Size: 778 190 792 2127
Fragments arranged by size:
2127 792 778 190
BsmI GAATG_Cn'
Cuts at: 638 638
Size: 3887
Bsp1286I G_dGCh'C
Cuts at: 172 340 901 1512 1605 1679 1869 1931 2661
Size: 168 561 611 93 74 190 62 730
Cuts at: 2661 172
Size: 1398
Fragments arranged by size:
1398 730 611 561 190 168 93 74
62
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 937 1151 1453 1834 937
Size: 214 302 381 2990
Fragments arranged by size:
2990 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 154 1885 2066 2275 154
Size: 1731 181 209 1766
Fragments arranged by size:
1766 1731 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 539 640 711 912 1379 1402 1737 1983
Size: 538 101 71 201 467 23 335 246
Cuts at: 1983 2995 3081 3093 3439 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 538 467 449 346 335 246 201
101 86 71 23 12
Bsu36I CC'TnA_GG
Cuts at: 435 435
Size: 3887
BtgI C'CryG_G
Cuts at: 107 107
Size: 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 725 1472 1646 2037 2064 3409 725
Size: 747 174 391 27 1345 1203
Fragments arranged by size:
1345 1203 747 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 176 1910 2120 176
Size: 1734 210 1943
Fragments arranged by size:
1943 1734 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 1712 2144 2891 3159 200
Size: 1512 432 747 268 928
Fragments arranged by size:
1512 928 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1026 1026
Size: 3887
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 498 1906 498
Size: 1408 2479
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 1321 1569 2591 3570 1321
Size: 248 1022 979 1638
Fragments arranged by size:
1638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 703 735 1729 1759 1761 1791 418
Size: 32 253 32 994 30 2 30 2514
Fragments arranged by size:
2514 994 253 32 32 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
HindIII A'AGCT_T
Cuts at: 249 249
Size: 3887
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 458 3320 458
Size: 2862 1025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 727 1648 727
Size: 921 2966
MslI CAynn'nnrTG
Cuts at: 461 1876 2003 461
Size: 1415 127 2345
Fragments arranged by size:
2345 1415 127
MspA1I CmG'CkG
Cuts at: 533 998 1312 1672 2685 2930 533
Size: 465 314 360 1013 245 1490
Fragments arranged by size:
1490 1013 465 360 314 245
NaeI GCC'GGC
Cuts at: 156 2068 156
Size: 1912 1975
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NcoI C'CATG_G
Cuts at: 107 107
Size: 3887
NgoMIV G'CCGG_C
Cuts at: 154 2066 154
Size: 1912 1975
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PspXI vC'TCGA_Gb
Cuts at: 148 148
Size: 3887
PstI C_TGCA'G
Cuts at: 711 934 1619 711
Size: 223 685 2979
Fragments arranged by size:
2979 685 223
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 533 1312 1672 533
Size: 779 360 2748
Fragments arranged by size:
2748 779 360
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 707 930 1615 2608 2799 33
Size: 674 223 685 993 191 1121
Fragments arranged by size:
1121 993 685 674 223 191
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 784 909 784
Size: 125 3762
SmlI C'TyrA_G
Cuts at: 148 502 1042 1957 2449 2711 148
Size: 354 540 915 492 262 1324
Fragments arranged by size:
1324 915 540 492 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StuI AGG'CCT
Cuts at: 433 745 433
Size: 312 3575
StyI C'CwwG_G
Cuts at: 107 1021 1375 107
Size: 914 354 2619
Fragments arranged by size:
2619 914 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 1470 1603 2062 2271 294
Size: 1176 133 459 209 1910
Fragments arranged by size:
1910 1176 459 209 133
TatI w'GTAC_w
Cuts at: 3402 3402
Size: 3887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 162 918 162
Size: 756 3131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 1382
Size: 3887
XhoI C'TCGA_G
Cuts at: 148 148
Size: 3887
XmaI C'CCGG_G
Cuts at: 782 907 782
Size: 125 3762
XmnI GAAnn'nnTTC
Cuts at: 757 757
Size: 3887
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI