Restriction map for
pF1KE2534
(Circular) (Six-base) MAPSORT of: pF1KE2534.seq Check: 4183 from: 1 to: 4145
pF1KE2534 4145 bp
With 182 enzymes: SgfI *
July 9, 2014 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 912 912
Size: 4145
AccI GT'mk_AC
Cuts at: 527 1183 527
Size: 656 3489
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1970 2402 3149 3417 1970
Size: 432 747 268 2698
Fragments arranged by size:
2698 747 432 268
AflIII A'CryG_T
Cuts at: 1497 2601 3578 1497
Size: 1104 977 2064
Fragments arranged by size:
2064 1104 977
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AlwNI CAG_nnn'CTG
Cuts at: 1024 1063 1450 3017 1024
Size: 39 387 1567 2152
Fragments arranged by size:
2152 1567 387 39
ApaI G_GGCC'C
Cuts at: 386 386
Size: 4145
ApaLI G'TGCA_C
Cuts at: 2915 2915
Size: 4145
ApoI r'AATT_y
Cuts at: 1149 3498 3754 1149
Size: 2349 256 1540
Fragments arranged by size:
2349 1540 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 1165 1165
Size: 4145
BamHI G'GATC_C
Cuts at: 739 991 1170 739
Size: 252 179 3714
Fragments arranged by size:
3714 252 179
BanI G'GyrC_C
Cuts at: 1161 1575 1823 1858 1161
Size: 414 248 35 3448
Fragments arranged by size:
3448 414 248 35
BanII G_rGCy'C
Cuts at: 386 959 1159 2189 386
Size: 573 200 1030 2342
Fragments arranged by size:
2342 1030 573 200
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BbvCI CC'TCA_GC
Cuts at: 120 120
Size: 4145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1098 1132 1098
Size: 34 4111
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 1052 1665 1052
Size: 613 3532
BfrBI ATG'CAT
Cuts at: 421 421
Size: 4145
BglI GCCn_nnn'nGGC
Cuts at: 1129 1129
Size: 4145
BglII A'GATC_T
Cuts at: 489 1660 3697 489
Size: 1171 2037 937
Fragments arranged by size:
2037 1171 937
BlpI GC'TnA_GC
Cuts at: 1257 1257
Size: 4145
Bme1580I G_kGCm'C
Cuts at: 386 1770 1863 2919 386
Size: 1384 93 1056 1612
Fragments arranged by size:
1612 1384 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 784 1357 1438 1773 3917 784
Size: 573 81 335 2144 1012
Fragments arranged by size:
2144 1012 573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 540
Size: 4145
Bpu10I CC'TnA_GC
Cuts at: 120 120
Size: 4145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 2200 2692 2990 1321
Size: 879 492 298 2476
Fragments arranged by size:
2476 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3849 3849
Size: 4145
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 1659 3696 6
Size: 1653 2037 455
Fragments arranged by size:
2037 1653 455
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 511 1334 1855 2533 2807 2954 3365 511
Size: 823 521 678 274 147 411 1291
Fragments arranged by size:
1291 823 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 765 1570 2328 2905 3401 765
Size: 805 758 577 496 1509
Fragments arranged by size:
1509 805 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 890
Size: 4145
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 959 1074 1159 1937 2127 2919 959
Size: 115 85 778 190 792 2185
Fragments arranged by size:
2185 792 778 190 115 85
Bsp1286I G_dGCh'C
Cuts at: 386 959 1074 1159 1770 1863 1937 2127 2189
Size: 573 115 85 611 93 74 190 62
Cuts at: 2189 2919 386
Size: 730 1612
Fragments arranged by size:
1612 730 611 573 190 115 93 85
74 62
BspEI T'CCGG_A
Cuts at: 3365 3365
Size: 4145
BspHI T'CATG_A
Cuts at: 3321 3321
Size: 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 912 1195 1409 1711 2092 287
Size: 625 283 214 302 381 2340
Fragments arranged by size:
2340 625 381 302 283 214
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2057 2057
Size: 4145
BsrFI r'CCGG_y
Cuts at: 2143 2324 2533 2143
Size: 181 209 3755
Fragments arranged by size:
3755 209 181
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 1522 1522
Size: 4145
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 4145
BstYI r'GATC_y
Cuts at: 1 489 739 991 1170 1637 1660 1995 2241
Size: 488 250 252 179 467 23 335 246
Cuts at: 2241 3253 3339 3351 3697 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 488 467 449 346 335 252 250
246 179 86 23 12
BstZ17I GTA'TAC
Cuts at: 528 528
Size: 4145
Bsu36I CC'TnA_GG
Cuts at: 614 614
Size: 4145
BtgI C'CryG_G
Cuts at: 107 575 107
Size: 468 3677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2095 2276 2439 2095
Size: 181 163 3801
Fragments arranged by size:
3801 181 163
BtsI GCAGTG_nn'
Cuts at: 203 789 1388 203
Size: 586 599 2960
Fragments arranged by size:
2960 599 586
DraI TTT'AAA
Cuts at: 1144 1144
Size: 4145
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 1091 1121 1730 1904 2295 2322 3667 1091
Size: 30 609 174 391 27 1345 1569
Fragments arranged by size:
1569 1345 609 391 174 30 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 957 1157 957
Size: 200 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1970 2402 3149 3417 540
Size: 1430 432 747 268 1268
Fragments arranged by size:
1430 1268 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 112 159 382 1284 112
Size: 47 223 902 2973
Fragments arranged by size:
2973 902 223 47
EcoRI G'AATT_C
Cuts at: 1149 1149
Size: 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 4145
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1579 1827 2849 3828 1579
Size: 248 1022 979 1896
Fragments arranged by size:
1896 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 693 724 725 756 1987 2017 2019
Size: 32 533 31 1 31 1231 30 2
Cuts at: 2019 2049 128
Size: 30 2224
Fragments arranged by size:
2224 1231 533 32 31 31 30 30
2 1
HincII GTy'rAC
Cuts at: 1184 1184
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 2816 3000 593
Size: 2223 184 1738
Fragments arranged by size:
2223 1738 184
MscI TGG'CCA
Cuts at: 1093 1123 1906 1093
Size: 30 783 3332
Fragments arranged by size:
3332 783 30
MslI CAynn'nnrTG
Cuts at: 976 2134 2261 976
Size: 1158 127 2860
Fragments arranged by size:
2860 1158 127
MspA1I CmG'CkG
Cuts at: 305 684 1087 1256 1570 1930 2943 3188 305
Size: 379 403 169 314 360 1013 245 1262
Fragments arranged by size:
1262 1013 403 379 360 314 245 169
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 107 107
Size: 4145
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NsiI A_TGCA'T
Cuts at: 423 423
Size: 4145
NspI r_CATG'y
Cuts at: 145 1198 1698 2605 145
Size: 1053 500 907 1685
Fragments arranged by size:
1685 1053 907 500
PasI CC'CwG_GG
Cuts at: 379 379
Size: 4145
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PpuMI rG'GwC_Cy
Cuts at: 112 159 112
Size: 47 4098
PshAI GACnn'nnGTC
Cuts at: 1042 1042
Size: 4145
PspOMI G'GGCC_C
Cuts at: 382 382
Size: 4145
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 616 648 616
Size: 32 4113
PstI C_TGCA'G
Cuts at: 136 683 1192 1877 136
Size: 547 509 685 2404
Fragments arranged by size:
2404 685 547 509
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 305 684 1087 1570 1930 305
Size: 379 403 483 360 2520
Fragments arranged by size:
2520 483 403 379 360
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 959 1159 959
Size: 200 3945
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SanDI GG'GwC_CC
Cuts at: 112 112
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 913 3662 913
Size: 2749 1396
SfcI C'TryA_G
Cuts at: 33 132 679 1188 1873 2866 3057 33
Size: 99 547 509 685 993 191 1121
Fragments arranged by size:
1121 993 685 547 509 191 99
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 1300 2215 2707 2969 1300
Size: 915 492 262 2476
Fragments arranged by size:
2476 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
StyI C'CwwG_G
Cuts at: 107 1279 1633 107
Size: 1172 354 2619
Fragments arranged by size:
2619 1172 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 213 460 1728 1861 2320 2529 213
Size: 247 1268 133 459 209 1829
Fragments arranged by size:
1829 1268 459 247 209 133
TatI w'GTAC_w
Cuts at: 911 3660 911
Size: 2749 1396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4113
Tth111I GACn'n_nGTC
Cuts at: 790 1942 790
Size: 1152 2993
XbaI T'CTAG_A
Cuts at: 1176 1176
Size: 4145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 292 1640 292
Size: 1348 2797
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BmgBI BmtI BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PflMI PfoI PmlI PsiI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI