Restriction map for
pF1KE2527
(Circular) (Six-base) MAPSORT of: pF1KE2527.seq Check: 6616 from: 1 to: 8723
pF1KE2527 8723 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4921 4921
Size: 8723
AatII G_ACGT'C
Cuts at: 4209 4209
Size: 8723
AccI GT'mk_AC
Cuts at: 843 5103 5592 5761 843
Size: 4260 489 169 3805
Fragments arranged by size:
4260 3805 489 169
Acc65I G'GTAC_C
Cuts at: 5739 5739
Size: 8723
AclI AA'CG_TT
Cuts at: 178 2815 8563 178
Size: 2637 5748 338
Fragments arranged by size:
5748 2637 338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 1654 2009 3331 3346 3409 3602 3676 3908
Size: 881 355 1322 15 63 193 74 232
Cuts at: 3908 4384 4418 4731 5408 6548 6980 7727 7995
Size: 476 34 313 677 1140 432 747 268
Cuts at: 7995 773
Size: 1501
Fragments arranged by size:
1501 1322 1140 881 747 677 476 432
355 313 268 232 193 74 63 34
15
AflII C'TTAA_G
Cuts at: 1815 2317 2998 1815
Size: 502 681 7540
Fragments arranged by size:
7540 681 502
AflIII A'CryG_T
Cuts at: 6075 7179 8156 6075
Size: 1104 977 6642
Fragments arranged by size:
6642 1104 977
AgeI A'CCGG_T
Cuts at: 7111 7111
Size: 8723
AleI CACnn'nnGTG
Cuts at: 4970 4970
Size: 8723
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4826 4858 4826
Size: 32 8691
AlwNI CAG_nnn'CTG
Cuts at: 3814 5626 6028 7595 3814
Size: 1812 402 1567 4942
Fragments arranged by size:
4942 1812 1567 402
ApaLI G'TGCA_C
Cuts at: 7493 7493
Size: 8723
ApoI r'AATT_y
Cuts at: 119 367 526 539 996 1126 1683 1749 1877
Size: 248 159 13 457 130 557 66 128
Cuts at: 1877 1927 2100 2193 2688 3435 3912 3967 4059
Size: 50 173 93 495 747 477 55 92
Cuts at: 4059 4094 4249 4635 4978 5682 5727 8076 8332
Size: 35 155 386 343 704 45 2349 256
Cuts at: 8332 119
Size: 510
Fragments arranged by size:
2349 747 704 557 510 495 477 457
386 343 256 248 173 159 155 130
128 93 92 66 55 50 45 35
13
AseI AT'TA_AT
Cuts at: 20 272 1330 3247 4105 4334 20
Size: 252 1058 1917 858 229 4409
Fragments arranged by size:
4409 1917 1058 858 252 229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8723
AvaI C'yCGr_G
Cuts at: 5743 5743
Size: 8723
BamHI G'GATC_C
Cuts at: 5748 5748
Size: 8723
BanI G'GyrC_C
Cuts at: 5739 6153 6401 6436 5739
Size: 414 248 35 8026
Fragments arranged by size:
8026 414 248 35
BanII G_rGCy'C
Cuts at: 2336 3163 5737 6767 2336
Size: 827 2574 1030 4292
Fragments arranged by size:
4292 2574 1030 827
BbeI G_GCGC'C
Cuts at: 6157 6405 6157
Size: 248 8475
BbsI GAAGACnn'nnnn_
Cuts at: 1166 1205 1660 1802 2863 3188 3494 3551 3707
Size: 39 455 142 1061 325 306 57 156
Cuts at: 3707 3916 4799 1166
Size: 209 883 5090
Fragments arranged by size:
5090 1061 883 455 325 306 209 156
142 57 39
BciVI GTATCCnnnnn_n'
Cuts at: 6621 7382 8723 6621
Size: 761 1341 6621
Fragments arranged by size:
6621 1341 761
BclI T'GATC_A
Cuts at: 398 3572 6243 398
Size: 3174 2671 2878
Fragments arranged by size:
3174 2878 2671
BglII A'GATC_T
Cuts at: 6238 8275 6238
Size: 2037 6686
BlpI GC'TnA_GC
Cuts at: 1715 1915 2270 4042 5835 1715
Size: 200 355 1772 1793 4603
Fragments arranged by size:
4603 1793 1772 355 200
Bme1580I G_kGCm'C
Cuts at: 6348 6441 7497 6348
Size: 93 1056 7574
Fragments arranged by size:
7574 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 5059 5935 6016 6351 8495 5059
Size: 876 81 335 2144 5287
Fragments arranged by size:
5287 2144 876 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 7090 3182
Size: 3908 4815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 803 4368 5056 5116 157
Size: 646 3565 688 60 3764
Fragments arranged by size:
3764 3565 688 646 60
Bpu10I CC'TnA_GC
Cuts at: 895 895
Size: 8723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 2923 3340 3697 5190 5420 5899 6778 7270
Size: 1953 417 357 1493 230 479 879 492
Cuts at: 7270 7568 970
Size: 298 2125
Fragments arranged by size:
2125 1953 1493 879 492 479 417 357
298 230
BsaI GGTCTCn'nnnn_
Cuts at: 772 3686 5361 8427 772
Size: 2914 1675 3066 1068
Fragments arranged by size:
3066 2914 1675 1068
BsaAI yAC'GTr
Cuts at: 2830 6076 2830
Size: 3246 5477
BsaBI GATnn'nnATC
Cuts at: 6 4334 6237 8274 6
Size: 4328 1903 2037 455
Fragments arranged by size:
4328 2037 1903 455
BsaHI Gr'CG_yC
Cuts at: 4206 6154 6402 7104 8600 4206
Size: 1948 248 702 1496 4329
Fragments arranged by size:
4329 1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5912 6433 7111 7385 7532 7943 5912
Size: 521 678 274 147 411 6692
Fragments arranged by size:
6692 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3155 4256 3155
Size: 1101 7622
BseYI C'CCAG_C
Cuts at: 1397 6148 6906 7483 7979 1397
Size: 4751 758 577 496 2141
Fragments arranged by size:
4751 2141 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 782 1859 4612 4955 521
Size: 261 1077 2753 343 4289
Fragments arranged by size:
4289 2753 1077 343 261
BsiEI CG_ry'CG
Cuts at: 104 6265 6311 7519 104
Size: 6161 46 1208 1308
Fragments arranged by size:
6161 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1224 2336 3163 5072 5737 6515 6705 7497 1224
Size: 1112 827 1909 665 778 190 792 2450
Fragments arranged by size:
2450 1909 1112 827 792 778 665 190
BsmI GAATG_Cn'
Cuts at: 338 3420 3483 338
Size: 3082 63 5578
Fragments arranged by size:
5578 3082 63
Bsp1286I G_dGCh'C
Cuts at: 1224 2336 3163 5072 5737 6348 6441 6515 6705
Size: 1112 827 1909 665 611 93 74 190
Cuts at: 6705 6767 7497 1224
Size: 62 730 2450
Fragments arranged by size:
2450 1909 1112 827 730 665 611 190
93 74 62
BspEI T'CCGG_A
Cuts at: 7943 7943
Size: 8723
BspHI T'CATG_A
Cuts at: 146 401 1281 1884 2895 3467 3582 3603 3801
Size: 255 880 603 1011 572 115 21 198
Cuts at: 3801 4992 7899 146
Size: 1191 2907 970
Fragments arranged by size:
2907 1191 1011 970 880 603 572 255
198 115 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 4921 5684 5773 5987 6289 6670 632
Size: 4289 763 89 214 302 381 2685
Fragments arranged by size:
4289 2685 763 381 302 214 89
BsrBI CCG'CTC
Cuts at: 50 759 4376 7015 7069 8553 8718 50
Size: 709 3617 2639 54 1484 165 55
Fragments arranged by size:
3617 2639 1484 709 165 55 54
BsrDI GCAATG_nn'
Cuts at: 634 688 1578 2491 6635 634
Size: 54 890 913 4144 2722
Fragments arranged by size:
4144 2722 913 890 54
BsrFI r'CCGG_y
Cuts at: 6721 6902 7111 6721
Size: 181 209 8333
Fragments arranged by size:
8333 209 181
BssSI C'ACGA_G
Cuts at: 1217 5513 6994 7352 1217
Size: 4296 1481 358 2588
Fragments arranged by size:
4296 2588 1481 358
BstAPI GCAn_nnn'nTGC
Cuts at: 574 6100 574
Size: 5526 3197
BstBI TT'CG_AA
Cuts at: 7084 7084
Size: 8723
BstXI CCAn_nnnn'nTGG
Cuts at: 159 4761 4972 159
Size: 4602 211 3910
Fragments arranged by size:
4602 3910 211
BstYI r'GATC_y
Cuts at: 1 1695 4152 4750 5748 6215 6238 6573 6819
Size: 1694 2457 598 998 467 23 335 246
Cuts at: 6819 7831 7917 7929 8275 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2457 1694 1012 998 598 467 449 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 4598 107
Size: 4491 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6673 6854 7017 6673
Size: 181 163 8379
Fragments arranged by size:
8379 181 163
BtsI GCAGTG_nn'
Cuts at: 3274 4977 5022 5642 5966 3274
Size: 1703 45 620 324 6031
Fragments arranged by size:
6031 1703 620 324 45
DraI TTT'AAA
Cuts at: 2060 2154 3878 5722 2060
Size: 94 1724 1844 5061
Fragments arranged by size:
5061 1844 1724 94
DraIII CAC_nnn'GTG
Cuts at: 811 2830 4810 811
Size: 2019 1980 4724
Fragments arranged by size:
4724 2019 1980
DrdI GACnn_nn'nnGTC
Cuts at: 648 6429 7287 648
Size: 5781 858 2084
Fragments arranged by size:
5781 2084 858
EaeI y'GGCC_r
Cuts at: 191 4461 5297 6308 6482 6873 6900 8245 191
Size: 4270 836 1011 174 391 27 1345 669
Fragments arranged by size:
4270 1345 1011 836 669 391 174 27
EagI C'GGCC_G
Cuts at: 6308 6308
Size: 8723
EarI CTCTTCn'nnn_
Cuts at: 749 795 1532 3407 4220 4740 5259 6746 6956
Size: 46 737 1875 813 520 519 1487 210
Cuts at: 6956 749
Size: 2516
Fragments arranged by size:
2516 1875 1487 813 737 520 519 210
46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6931 7241 7387 8533 6931
Size: 310 146 1146 7121
Fragments arranged by size:
7121 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2334 3161 5735 2334
Size: 827 2574 5322
Fragments arranged by size:
5322 2574 827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 773 803 1654 2009 3331 3346 3409 3602
Size: 616 30 851 355 1322 15 63 193
Cuts at: 3602 3676 3908 4368 4384 4418 4731 5056 5116
Size: 74 232 460 16 34 313 325 60
Cuts at: 5116 5408 6548 6980 7727 7995 157
Size: 292 1140 432 747 268 885
Fragments arranged by size:
1322 1140 885 851 747 616 460 432
355 325 313 292 268 232 193 74
63 60 34 30 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4518 5426 4518
Size: 908 7815
EcoO109I rG'GnC_Cy
Cuts at: 5862 5862
Size: 8723
EcoRI G'AATT_C
Cuts at: 539 2193 5727 539
Size: 1654 3534 3535
Fragments arranged by size:
3535 3534 1654
EcoRV GAT'ATC
Cuts at: 1415 1415
Size: 8723
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1294 1735 2332 4414 5236 6742 1294
Size: 441 597 2082 822 1506 3275
Fragments arranged by size:
3275 2082 1506 822 597 441
FspI TGC'GCA
Cuts at: 6504 6504
Size: 8723
HaeII r_GCGC'y
Cuts at: 6157 6405 7427 8406 6157
Size: 248 1022 979 6474
Fragments arranged by size:
6474 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1000 1032 1465 1497 1529 2469 2501 3538 3564
Size: 32 433 32 32 940 32 1037 26
Cuts at: 3564 3570 3596 3613 3645 3994 4026 4768 4800
Size: 6 26 17 32 349 32 742 32
Cuts at: 4800 4993 5025 5091 5123 5358 5390 6565 6595
Size: 193 32 66 32 235 32 1175 30
Cuts at: 6595 6597 6627 1000
Size: 2 30 3096
Fragments arranged by size:
3096 1175 1037 940 742 433 349 235
193 66 32 32 32 32 32 32
32 32 32 32 30 30 26 26
17 6 2
HincII GTy'rAC
Cuts at: 844 1478 3871 4731 5104 5762 844
Size: 634 2393 860 373 658 3805
Fragments arranged by size:
3805 2393 860 658 634 373
HindIII A'AGCT_T
Cuts at: 1078 3421 5179 1078
Size: 2343 1758 4622
Fragments arranged by size:
4622 2343 1758
KasI G'GCGC_C
Cuts at: 6153 6401 6153
Size: 248 8475
KpnI G_GTAC'C
Cuts at: 5743 5743
Size: 8723
MfeI C'AATT_G
Cuts at: 394 2605 3079 3997 394
Size: 2211 474 918 5120
Fragments arranged by size:
5120 2211 918 474
MluI A'CGCG_T
Cuts at: 8156 8156
Size: 8723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 911 1175 2655 3719 7394 7578 911
Size: 264 1480 1064 3675 184 2056
Fragments arranged by size:
3675 2056 1480 1064 264 184
MscI TGG'CCA
Cuts at: 4463 5299 6484 4463
Size: 836 1185 6702
Fragments arranged by size:
6702 1185 836
MslI CAynn'nnrTG
Cuts at: 352 2263 4576 4970 4997 6712 6839 352
Size: 1911 2313 394 27 1715 127 2236
Fragments arranged by size:
2313 2236 1911 1715 394 127 27
MspA1I CmG'CkG
Cuts at: 571 1824 2544 5623 5834 6148 6508 7521 7766
Size: 1253 720 3079 211 314 360 1013 245
Cuts at: 7766 571
Size: 1528
Fragments arranged by size:
3079 1528 1253 1013 720 360 314 245
211
NaeI GCC'GGC
Cuts at: 6904 6904
Size: 8723
NarI GG'CG_CC
Cuts at: 6154 6402 6154
Size: 248 8475
NcoI C'CATG_G
Cuts at: 107 4598 107
Size: 4491 4232
NgoMIV G'CCGG_C
Cuts at: 6902 6902
Size: 8723
NspI r_CATG'y
Cuts at: 5776 6276 7183 5776
Size: 500 907 7316
Fragments arranged by size:
7316 907 500
PasI CC'CwG_GG
Cuts at: 1455 5323 1455
Size: 3868 4855
PciI A'CATG_T
Cuts at: 7179 7179
Size: 8723
PflMI CCAn_nnn'nTGG
Cuts at: 3175 3999 4470 3175
Size: 824 471 7428
Fragments arranged by size:
7428 824 471
PmeI GTTT'AAAC
Cuts at: 5722 5722
Size: 8723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1206 1238 7862 7894 1206
Size: 32 6624 32 2035
Fragments arranged by size:
6624 2035 32 32
PshAI GACnn'nnGTC
Cuts at: 4799 4799
Size: 8723
PsiI TTA'TAA
Cuts at: 1133 1967 3071 1133
Size: 834 1104 6785
Fragments arranged by size:
6785 1104 834
PstI C_TGCA'G
Cuts at: 3819 4743 5649 5770 6455 3819
Size: 924 906 121 685 6087
Fragments arranged by size:
6087 924 906 685 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8723
PvuII CAG'CTG
Cuts at: 2544 5623 6148 6508 2544
Size: 3079 525 360 4759
Fragments arranged by size:
4759 3079 525 360
RsrII CG'GwC_CG
Cuts at: 6918 6918
Size: 8723
SacI G_AGCT'C
Cuts at: 2336 3163 5737 2336
Size: 827 2574 5322
Fragments arranged by size:
5322 2574 827
SalI G'TCGA_C
Cuts at: 842 5102 5760 842
Size: 4260 658 3805
Fragments arranged by size:
4260 3805 658
SapI GCTCTTCn'nnn_
Cuts at: 749 1532 6746 6956 749
Size: 783 5214 210 2516
Fragments arranged by size:
5214 2516 783 210
SbfI CC_TGCA'GG
Cuts at: 5770 5770
Size: 8723
ScaI AGT'ACT
Cuts at: 2952 4629 5038 8240 2952
Size: 1677 409 3202 3435
Fragments arranged by size:
3435 3202 1677 409
SexAI A'CCwGG_T
Cuts at: 254 4586 5370 254
Size: 4332 784 3607
Fragments arranged by size:
4332 3607 784
SfcI C'TryA_G
Cuts at: 33 1562 2275 3815 3864 4111 4706 4739 5645
Size: 1529 713 1540 49 247 595 33 906
Cuts at: 5645 5766 6451 7444 7635 33
Size: 121 685 993 191 1121
Fragments arranged by size:
1540 1529 1121 993 906 713 685 595
247 191 121 49 33
SfoI GGC'GCC
Cuts at: 6155 6403 6155
Size: 248 8475
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8723
SmaI CCC'GGG
Cuts at: 5745 5745
Size: 8723
SmlI C'TyrA_G
Cuts at: 949 1815 2317 2902 2998 3319 3676 5205 5435
Size: 866 502 585 96 321 357 1529 230
Cuts at: 5435 5878 6793 7285 7547 949
Size: 443 915 492 262 2125
Fragments arranged by size:
2125 1529 915 866 585 502 492 443
357 321 262 230 96
SpeI A'CTAG_T
Cuts at: 67 1423 67
Size: 1356 7367
SphI G_CATG'C
Cuts at: 5776 6276 5776
Size: 500 8223
StuI AGG'CCT
Cuts at: 2086 4555 2086
Size: 2469 6254
StyI C'CwwG_G
Cuts at: 107 4469 4598 5582 5857 6211 107
Size: 4362 129 984 275 354 2619
Fragments arranged by size:
4362 2619 984 354 275 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4383 4545 6306 6439 6898 7107 4383
Size: 162 1761 133 459 209 5999
Fragments arranged by size:
5999 1761 459 209 162 133
TatI w'GTAC_w
Cuts at: 2950 4178 4627 5036 5190 5667 8238 2950
Size: 1228 449 409 154 477 2571 3435
Fragments arranged by size:
3435 2571 1228 477 449 409 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 8240 8272 328
Size: 32 7880 32 779
Fragments arranged by size:
7880 779 32 32
Tth111I GACn'n_nGTC
Cuts at: 3375 5372 6520 3375
Size: 1997 1148 5578
Fragments arranged by size:
5578 1997 1148
XbaI T'CTAG_A
Cuts at: 5754 5754
Size: 8723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4584 6218 4584
Size: 1634 7089
XmaI C'CCGG_G
Cuts at: 5743 5743
Size: 8723
XmnI GAAnn'nnTTC
Cuts at: 539 1541 3599 4394 539
Size: 1002 2058 795 4868
Fragments arranged by size:
4868 2058 1002 795
ZraI GAC'GTC
Cuts at: 4207 4207
Size: 8723
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI