Restriction map for
pF1KE2525
(Circular) (Six-base) MAPSORT of: pF1KE2525.seq Check: 5466 from: 1 to: 4679
pF1KE2525 4679 bp
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AccI GT'mk_AC
Cuts at: 1184 1717 1184
Size: 533 4146
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1338 1634 2504 2936 3683 3951 997
Size: 341 296 870 432 747 268 1725
Fragments arranged by size:
1725 870 747 432 341 296 268
AflIII A'CryG_T
Cuts at: 2031 3135 4112 2031
Size: 1104 977 2598
Fragments arranged by size:
2598 1104 977
AgeI A'CCGG_T
Cuts at: 3067 3067
Size: 4679
AleI CACnn'nnGTG
Cuts at: 412 412
Size: 4679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 500 1595 500
Size: 1095 3584
AlwNI CAG_nnn'CTG
Cuts at: 1984 3551 1984
Size: 1567 3112
ApaI G_GGCC'C
Cuts at: 271 271
Size: 4679
ApaLI G'TGCA_C
Cuts at: 3449 3449
Size: 4679
ApoI r'AATT_y
Cuts at: 469 517 1189 1683 4032 4288 469
Size: 48 672 494 2349 256 860
Fragments arranged by size:
2349 860 672 494 256 48
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 122 199 327 1699 122
Size: 77 128 1372 3102
Fragments arranged by size:
3102 1372 128 77
BamHI G'GATC_C
Cuts at: 851 1535 1704 851
Size: 684 169 3826
Fragments arranged by size:
3826 684 169
BanI G'GyrC_C
Cuts at: 286 367 748 1695 2109 2357 2392 286
Size: 81 381 947 414 248 35 2573
Fragments arranged by size:
2573 947 414 381 248 81 35
BanII G_rGCy'C
Cuts at: 129 206 271 1693 2723 129
Size: 77 65 1422 1030 2085
Fragments arranged by size:
2085 1422 1030 77 65
BbeI G_GCGC'C
Cuts at: 290 2113 2361 290
Size: 1823 248 2608
Fragments arranged by size:
2608 1823 248
BbsI GAAGACnn'nnnn_
Cuts at: 1344 1435 1344
Size: 91 4588
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 349 383 349
Size: 34 4645
BciVI GTATCCnnnnn_n'
Cuts at: 2577 3338 4679 2577
Size: 761 1341 2577
Fragments arranged by size:
2577 1341 761
BclI T'GATC_A
Cuts at: 480 926 2199 480
Size: 446 1273 2960
Fragments arranged by size:
2960 1273 446
BfrBI ATG'CAT
Cuts at: 477 477
Size: 4679
BglII A'GATC_T
Cuts at: 2194 4231 2194
Size: 2037 2642
BlpI GC'TnA_GC
Cuts at: 1791 1791
Size: 4679
Bme1580I G_kGCm'C
Cuts at: 271 2304 2397 3453 271
Size: 2033 93 1056 1497
Fragments arranged by size:
2033 1497 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1156 1891 1972 2307 4451 1156
Size: 735 81 335 2144 1384
Fragments arranged by size:
2144 1384 735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 4679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1241 973
Size: 268 4411
Bpu10I CC'TnA_GC
Cuts at: 130 756 130
Size: 626 4053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1855 2734 3226 3524 1855
Size: 879 492 298 3010
Fragments arranged by size:
3010 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4383 4383
Size: 4679
BsaAI yAC'GTr
Cuts at: 2032 2032
Size: 4679
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 287 2110 2358 3060 4556 287
Size: 1823 248 702 1496 410
Fragments arranged by size:
1823 1496 702 410 248
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3341 3488 3899 1868
Size: 521 678 274 147 411 2648
Fragments arranged by size:
2648 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 961 961
Size: 4679
BseYI C'CCAG_C
Cuts at: 363 399 2104 2862 3439 3935 363
Size: 36 1705 758 577 496 1107
Fragments arranged by size:
1705 1107 758 577 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 924 842
Size: 82 4597
BsiEI CG_ry'CG
Cuts at: 104 359 804 2221 2267 3475 104
Size: 255 445 1417 46 1208 1308
Fragments arranged by size:
1417 1308 1208 445 255 46
BsiHKAI G_wGCw'C
Cuts at: 1693 2471 2661 3453 1693
Size: 778 190 792 2919
Fragments arranged by size:
2919 792 778 190
BsmI GAATG_Cn'
Cuts at: 576 576
Size: 4679
Bsp1286I G_dGCh'C
Cuts at: 129 206 271 1693 2304 2397 2471 2661 2723
Size: 77 65 1422 611 93 74 190 62
Cuts at: 2723 3453 129
Size: 730 1355
Fragments arranged by size:
1422 1355 730 611 190 93 77 74
65 62
BspEI T'CCGG_A
Cuts at: 3899 3899
Size: 4679
BspHI T'CATG_A
Cuts at: 729 1064 3855 729
Size: 335 2791 1553
Fragments arranged by size:
2791 1553 335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 814 1729 1943 2245 2626 814
Size: 915 214 302 381 2867
Fragments arranged by size:
2867 915 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 830 2591 830
Size: 1761 2918
BsrFI r'CCGG_y
Cuts at: 136 2677 2858 3067 136
Size: 2541 181 209 1748
Fragments arranged by size:
2541 1748 209 181
BssHII G'CGCG_C
Cuts at: 166 168 219 344 346 166
Size: 2 51 125 2 4499
Fragments arranged by size:
4499 125 51 2 2
BssSI C'ACGA_G
Cuts at: 636 2950 3308 636
Size: 2314 358 2007
Fragments arranged by size:
2314 2007 358
BstAPI GCAn_nnn'nTGC
Cuts at: 938 1270 2056 938
Size: 332 786 3561
Fragments arranged by size:
3561 786 332
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstYI r'GATC_y
Cuts at: 1 740 851 1535 1704 2171 2194 2529 2775
Size: 739 111 684 169 467 23 335 246
Cuts at: 2775 3787 3873 3885 4231 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 739 684 467 449 346 335 246
169 111 86 23 12
BtgI C'CryG_G
Cuts at: 107 206 236 290 380 618 107
Size: 99 30 54 90 238 4168
Fragments arranged by size:
4168 238 99 90 54 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 785 2629 2810 2973 785
Size: 1844 181 163 2491
Fragments arranged by size:
2491 1844 181 163
BtsI GCAGTG_nn'
Cuts at: 1595 1922 1595
Size: 327 4352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 384 419 384
Size: 35 4644
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DraIII CAC_nnn'GTG
Cuts at: 1672 1672
Size: 4679
DrdI GACnn_nn'nnGTC
Cuts at: 1627 2385 3243 1627
Size: 758 858 3063
Fragments arranged by size:
3063 858 758
EaeI y'GGCC_r
Cuts at: 338 356 621 801 1275 2264 2438 2829 2856
Size: 18 265 180 474 989 174 391 27
Cuts at: 2856 4201 338
Size: 1345 816
Fragments arranged by size:
1345 989 816 474 391 265 180 174
27 18
EagI C'GGCC_G
Cuts at: 356 801 2264 356
Size: 445 1463 2771
Fragments arranged by size:
2771 1463 445
EarI CTCTTCn'nnn_
Cuts at: 1253 1649 1653 2702 2912 1253
Size: 396 4 1049 210 3020
Fragments arranged by size:
3020 1049 396 210 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2887 3197 3343 4489 2887
Size: 310 146 1146 3077
Fragments arranged by size:
3077 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1691 1691
Size: 4679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 997 1241 1338 1634 2504 2936 3683 3951
Size: 24 244 97 296 870 432 747 268
Cuts at: 3951 973
Size: 1701
Fragments arranged by size:
1701 870 747 432 296 268 244 97
24
EcoO109I rG'GnC_Cy
Cuts at: 267 1113 1128 1818 267
Size: 846 15 690 3128
Fragments arranged by size:
3128 846 690 15
EcoRI G'AATT_C
Cuts at: 469 1189 1683 469
Size: 720 494 3465
Fragments arranged by size:
3465 720 494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1267 1632 2698 1267
Size: 365 1066 3248
Fragments arranged by size:
3248 1066 365
FspI TGC'GCA
Cuts at: 2460 2460
Size: 4679
HaeII r_GCGC'y
Cuts at: 137 290 2113 2361 3383 4362 137
Size: 153 1823 248 1022 979 454
Fragments arranged by size:
1823 1022 979 454 248 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2521 2551 2553 2583 2521
Size: 30 2 30 4617
Fragments arranged by size:
4617 30 30 2
HincII GTy'rAC
Cuts at: 1086 1718 1086
Size: 632 4047
KasI G'GCGC_C
Cuts at: 286 2109 2357 286
Size: 1823 248 2608
Fragments arranged by size:
2608 1823 248
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MfeI C'AATT_G
Cuts at: 522 522
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 402 3350 3534 402
Size: 2948 184 1547
Fragments arranged by size:
2948 1547 184
MscI TGG'CCA
Cuts at: 340 623 2440 340
Size: 283 1817 2579
Fragments arranged by size:
2579 1817 283
MslI CAynn'nnrTG
Cuts at: 412 482 835 1069 1409 2668 2795 412
Size: 70 353 234 340 1259 127 2296
Fragments arranged by size:
2296 1259 353 340 234 127 70
MspA1I CmG'CkG
Cuts at: 208 280 457 786 1790 2104 2464 3477 3722
Size: 72 177 329 1004 314 360 1013 245
Cuts at: 3722 208
Size: 1165
Fragments arranged by size:
1165 1013 1004 360 329 314 245 177
72
NaeI GCC'GGC
Cuts at: 138 2860 138
Size: 2722 1957
NarI GG'CG_CC
Cuts at: 287 2110 2358 287
Size: 1823 248 2608
Fragments arranged by size:
2608 1823 248
NcoI C'CATG_G
Cuts at: 107 236 107
Size: 129 4550
NdeI CA'TA_TG
Cuts at: 1541 1541
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 136 2858 136
Size: 2722 1957
NsiI A_TGCA'T
Cuts at: 479 479
Size: 4679
NspI r_CATG'y
Cuts at: 1732 2232 3139 1732
Size: 500 907 3272
Fragments arranged by size:
3272 907 500
PasI CC'CwG_GG
Cuts at: 864 864
Size: 4679
PciI A'CATG_T
Cuts at: 3135 3135
Size: 4679
PflMI CCAn_nnn'nTGG
Cuts at: 399 399
Size: 4679
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4647
PpuMI rG'GwC_Cy
Cuts at: 1113 1128 1113
Size: 15 4664
PshAI GACnn'nnGTC
Cuts at: 1169 1169
Size: 4679
PsiI TTA'TAA
Cuts at: 1331 1331
Size: 4679
PspOMI G'GGCC_C
Cuts at: 267 267
Size: 4679
PstI C_TGCA'G
Cuts at: 247 1044 1574 1726 2411 247
Size: 797 530 152 685 2515
Fragments arranged by size:
2515 797 685 530 152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 457 786 2104 2464 457
Size: 329 1318 360 2672
Fragments arranged by size:
2672 1318 360 329
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1693 1693
Size: 4679
SacII CC_GC'GG
Cuts at: 209 209
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SanDI GG'GwC_CC
Cuts at: 1113 1113
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 1253 1649 2702 2912 1253
Size: 396 1053 210 3020
Fragments arranged by size:
3020 1053 396 210
SbfI CC_TGCA'GG
Cuts at: 247 1726 247
Size: 1479 3200
ScaI AGT'ACT
Cuts at: 4196 4196
Size: 4679
SexAI A'CCwGG_T
Cuts at: 308 308
Size: 4679
SfcI C'TryA_G
Cuts at: 33 243 1040 1570 1722 2407 3400 3591 33
Size: 210 797 530 152 685 993 191 1121
Fragments arranged by size:
1121 993 797 685 530 210 191 152
SfoI GGC'GCC
Cuts at: 288 2111 2359 288
Size: 1823 248 2608
Fragments arranged by size:
2608 1823 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SgrAI Cr'CCGG_yG
Cuts at: 136 136
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 1834 2749 3241 3503 1834
Size: 915 492 262 3010
Fragments arranged by size:
3010 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
StyI C'CwwG_G
Cuts at: 107 236 1124 1813 2167 107
Size: 129 888 689 354 2619
Fragments arranged by size:
2619 888 689 354 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 162 355 391 1544 2262 2395 2854 3063 162
Size: 193 36 1153 718 133 459 209 1778
Fragments arranged by size:
1778 1153 718 459 209 193 133 36
TatI w'GTAC_w
Cuts at: 677 4194 677
Size: 3517 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 627 659 4196 4228 627
Size: 32 3537 32 1078
Fragments arranged by size:
3537 1078 32 32
Tth111I GACn'n_nGTC
Cuts at: 1011 2476 1011
Size: 1465 3214
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 378 400 1366 2174 378
Size: 22 966 808 2883
Fragments arranged by size:
2883 966 808 22
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
XmnI GAAnn'nnTTC
Cuts at: 1400 1400
Size: 4679
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI PacI
PfoI PmlI PspXI PsrI SfiI SnaBI SrfI SspI
StuI SwaI XhoI ZraI