Restriction map for
pF1KE2523
(Circular) (Six-base) MAPSORT of: pF1KE2523.seq Check: 8535 from: 1 to: 6866
pF1KE2523 6866 bp
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1036 3684 1036
Size: 2648 4218
AatII G_ACGT'C
Cuts at: 704 704
Size: 6866
AccI GT'mk_AC
Cuts at: 2441 3904 2441
Size: 1463 5403
Acc65I G'GTAC_C
Cuts at: 1220 1305 3882 1220
Size: 85 2577 4204
Fragments arranged by size:
4204 2577 85
AclI AA'CG_TT
Cuts at: 6706 6706
Size: 6866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2590 2596 4691 5123 5870 6138 2378
Size: 212 6 2095 432 747 268 3106
Fragments arranged by size:
3106 2095 747 432 268 212 6
AfeI AGC'GCT
Cuts at: 3799 3799
Size: 6866
AflIII A'CryG_T
Cuts at: 637 3474 4218 5322 6299 637
Size: 2837 744 1104 977 1204
Fragments arranged by size:
2837 1204 1104 977 744
AgeI A'CCGG_T
Cuts at: 5254 5254
Size: 6866
AhdI GACnn_n'nnGTC
Cuts at: 216 1736 3315 216
Size: 1520 1579 3767
Fragments arranged by size:
3767 1579 1520
AleI CACnn'nnGTG
Cuts at: 541 541
Size: 6866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2715 2715
Size: 6866
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 3461 3493 646
Size: 32 2783 32 4019
Fragments arranged by size:
4019 2783 32 32
AlwNI CAG_nnn'CTG
Cuts at: 776 1051 4171 5738 776
Size: 275 3120 1567 1904
Fragments arranged by size:
3120 1904 1567 275
ApaLI G'TGCA_C
Cuts at: 5636 5636
Size: 6866
ApoI r'AATT_y
Cuts at: 355 686 820 983 2101 2531 3870 6219 6475
Size: 331 134 163 1118 430 1339 2349 256
Cuts at: 6475 355
Size: 746
Fragments arranged by size:
2349 1339 1118 746 430 331 256 163
134
AseI AT'TA_AT
Cuts at: 20 2390 20
Size: 2370 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6866
AvaI C'yCGr_G
Cuts at: 1441 2539 3886 1441
Size: 1098 1347 4421
Fragments arranged by size:
4421 1347 1098
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1289 1322 1289
Size: 33 6833
BamHI G'GATC_C
Cuts at: 3891 3891
Size: 6866
BanI G'GyrC_C
Cuts at: 1220 1305 1774 3300 3829 3882 4296 4544 4579
Size: 85 469 1526 529 53 414 248 35
Cuts at: 4579 1220
Size: 3507
Fragments arranged by size:
3507 1526 529 469 414 248 85 53
35
BanII G_rGCy'C
Cuts at: 3880 4910 3880
Size: 1030 5836
BbeI G_GCGC'C
Cuts at: 4300 4548 4300
Size: 248 6618
BbsI GAAGACnn'nnnn_
Cuts at: 1244 2234 2366 1244
Size: 990 132 5744
Fragments arranged by size:
5744 990 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1756 1790 1756
Size: 34 6832
BciVI GTATCCnnnnn_n'
Cuts at: 3372 4764 5525 6866 3372
Size: 1392 761 1341 3372
Fragments arranged by size:
3372 1392 1341 761
BclI T'GATC_A
Cuts at: 2807 4386 2807
Size: 1579 5287
BglI GCCn_nnn'nGGC
Cuts at: 2140 3820 2140
Size: 1680 5186
BglII A'GATC_T
Cuts at: 2288 4381 6418 2288
Size: 2093 2037 2736
Fragments arranged by size:
2736 2093 2037
BlpI GC'TnA_GC
Cuts at: 3978 3978
Size: 6866
Bme1580I G_kGCm'C
Cuts at: 548 1779 1858 4491 4584 5640 548
Size: 1231 79 2633 93 1056 1774
Fragments arranged by size:
2633 1774 1231 1056 93 79
BmgBI CAC'GTC
Cuts at: 638 2799 638
Size: 2161 4705
BmrI ACTGGGnnnn_n'
Cuts at: 1377 1861 4078 4159 4494 6638 1377
Size: 484 2217 81 335 2144 1605
Fragments arranged by size:
2217 2144 1605 484 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5233 5233
Size: 6866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2324
Size: 6866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4921 5413 5711 4042
Size: 879 492 298 5197
Fragments arranged by size:
5197 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6570 6570
Size: 6866
BsaAI yAC'GTr
Cuts at: 994 4219 994
Size: 3225 3641
BsaBI GATnn'nnATC
Cuts at: 6 4380 6417 6
Size: 4374 2037 455
Fragments arranged by size:
4374 2037 455
BsaHI Gr'CG_yC
Cuts at: 566 701 2438 4297 4545 5247 6743 566
Size: 135 1737 1859 248 702 1496 689
Fragments arranged by size:
1859 1737 1496 702 689 248 135
BsaWI w'CCGG_w
Cuts at: 1904 4055 4576 5254 5528 5675 6086 1904
Size: 2151 521 678 274 147 411 2684
Fragments arranged by size:
2684 2151 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3128 3158 3661 3691 3754 3784 3128
Size: 30 503 30 63 30 6210
Fragments arranged by size:
6210 503 63 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 2197 3091 3685 3762 277
Size: 1920 894 594 77 3381
Fragments arranged by size:
3381 1920 894 594 77
BseYI C'CCAG_C
Cuts at: 561 4291 5049 5626 6122 561
Size: 3730 758 577 496 1305
Fragments arranged by size:
3730 1305 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2485 2706 2485
Size: 221 6645
BsiEI CG_ry'CG
Cuts at: 104 318 4408 4454 5662 104
Size: 214 4090 46 1208 1308
Fragments arranged by size:
4090 1308 1208 214 46
BsiHKAI G_wGCw'C
Cuts at: 3101 3880 4658 4848 5640 3101
Size: 779 778 190 792 4327
Fragments arranged by size:
4327 792 779 778 190
BsmI GAATG_Cn'
Cuts at: 2858 3408 2858
Size: 550 6316
BsmBI CGTCTCn'nnnn_
Cuts at: 692 692
Size: 6866
Bsp1286I G_dGCh'C
Cuts at: 548 1779 1858 3101 3880 4491 4584 4658 4848
Size: 1231 79 1243 779 611 93 74 190
Cuts at: 4848 4910 5640 548
Size: 62 730 1774
Fragments arranged by size:
1774 1243 1231 779 730 611 190 93
79 74 62
BspEI T'CCGG_A
Cuts at: 6086 6086
Size: 6866
BspHI T'CATG_A
Cuts at: 1496 6042 1496
Size: 4546 2320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 650 1036 1382 3684 3916 4130 4432 4813 650
Size: 386 346 2302 232 214 302 381 2703
Fragments arranged by size:
2703 2302 386 381 346 302 232 214
BsrBI CCG'CTC
Cuts at: 50 5158 5212 6696 6861 50
Size: 5108 54 1484 165 55
Fragments arranged by size:
5108 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3332 3425 4778 3332
Size: 93 1353 5420
Fragments arranged by size:
5420 1353 93
BsrFI r'CCGG_y
Cuts at: 4864 5045 5254 4864
Size: 181 209 6476
Fragments arranged by size:
6476 209 181
BsrGI T'GTAC_A
Cuts at: 292 3080 292
Size: 2788 4078
BssSI C'ACGA_G
Cuts at: 5137 5495 5137
Size: 358 6508
BstAPI GCAn_nnn'nTGC
Cuts at: 2452 4243 2452
Size: 1791 5075
BstBI TT'CG_AA
Cuts at: 5227 5227
Size: 6866
BstXI CCAn_nnnn'nTGG
Cuts at: 2736 2772 3542 2736
Size: 36 770 6060
Fragments arranged by size:
6060 770 36
BstYI r'GATC_y
Cuts at: 1 1448 2288 3638 3891 4358 4381 4716 4962
Size: 1447 840 1350 253 467 23 335 246
Cuts at: 4962 5974 6060 6072 6418 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1447 1350 1012 840 467 449 346 335
253 246 86 23 12
BtgI C'CryG_G
Cuts at: 1026 1257 1301 2729 3833 1026
Size: 231 44 1428 1104 4059
Fragments arranged by size:
4059 1428 1104 231 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3634 4816 4997 5160 3634
Size: 1182 181 163 5340
Fragments arranged by size:
5340 1182 181 163
BtsI GCAGTG_nn'
Cuts at: 569 3682 4109 569
Size: 3113 427 3326
Fragments arranged by size:
3326 3113 427
ClaI AT'CG_AT
Cuts at: 3238 3238
Size: 6866
DraI TTT'AAA
Cuts at: 818 1092 1506 3865 818
Size: 274 414 2359 3819
Fragments arranged by size:
3819 2359 414 274
DraIII CAC_nnn'GTG
Cuts at: 2488 2488
Size: 6866
DrdI GACnn_nn'nnGTC
Cuts at: 4572 5430 4572
Size: 858 6008
EaeI y'GGCC_r
Cuts at: 1179 2774 2925 3619 4451 4625 5016 5043 6388
Size: 1595 151 694 832 174 391 27 1345
Cuts at: 6388 1179
Size: 1657
Fragments arranged by size:
1657 1595 1345 832 694 391 174 151
27
EagI C'GGCC_G
Cuts at: 4451 4451
Size: 6866
EarI CTCTTCn'nnn_
Cuts at: 491 2402 2743 3253 4889 5099 491
Size: 1911 341 510 1636 210 2258
Fragments arranged by size:
2258 1911 1636 510 341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3682 3691 3838 5074 5384 5530 6676 3682
Size: 9 147 1236 310 146 1146 3872
Fragments arranged by size:
3872 1236 1146 310 147 146 9
EcoICRI GAG'CTC
Cuts at: 3878 3878
Size: 6866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2378 2590 2596 4691 5123 5870 6138 2324
Size: 54 212 6 2095 432 747 268 3052
Fragments arranged by size:
3052 2095 747 432 268 212 54 6
EcoO109I rG'GnC_Cy
Cuts at: 1371 1823 3228 4005 1371
Size: 452 1405 777 4232
Fragments arranged by size:
4232 1405 777 452
EcoRI G'AATT_C
Cuts at: 3870 3870
Size: 6866
EcoRV GAT'ATC
Cuts at: 2040 2899 2040
Size: 859 6007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 220 983 3222 4885 220
Size: 763 2239 1663 2201
Fragments arranged by size:
2239 2201 1663 763
FspI TGC'GCA
Cuts at: 3035 4647 3035
Size: 1612 5254
HaeII r_GCGC'y
Cuts at: 3726 3801 4300 4548 5570 6549 3726
Size: 75 499 248 1022 979 4043
Fragments arranged by size:
4043 1022 979 499 248 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 1234 1266 1722 1754 2423 2455 3301
Size: 32 1000 32 456 32 669 32 846
Cuts at: 3301 3333 3754 3786 4708 4738 4740 4770 202
Size: 32 421 32 922 30 2 30 2298
Fragments arranged by size:
2298 1000 922 846 669 456 421 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 249 3548 3716 3905 249
Size: 3299 168 189 3210
Fragments arranged by size:
3299 3210 189 168
HindIII A'AGCT_T
Cuts at: 1618 1951 3213 1618
Size: 333 1262 5271
Fragments arranged by size:
5271 1262 333
HpaI GTT'AAC
Cuts at: 3548 3548
Size: 6866
KasI G'GCGC_C
Cuts at: 4296 4544 4296
Size: 248 6618
KpnI G_GTAC'C
Cuts at: 1224 1309 3886 1224
Size: 85 2577 4204
Fragments arranged by size:
4204 2577 85
MluI A'CGCG_T
Cuts at: 6299 6299
Size: 6866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 675 2811 3332 5537 5721 675
Size: 2136 521 2205 184 1820
Fragments arranged by size:
2205 2136 1820 521 184
MscI TGG'CCA
Cuts at: 2776 3621 4627 2776
Size: 845 1006 5015
Fragments arranged by size:
5015 1006 845
MslI CAynn'nnrTG
Cuts at: 541 1547 1717 2734 2944 4855 4982 541
Size: 1006 170 1017 210 1911 127 2425
Fragments arranged by size:
2425 1911 1017 1006 210 170 127
MspA1I CmG'CkG
Cuts at: 736 2652 3750 3835 3977 4291 4651 5664 5909
Size: 1916 1098 85 142 314 360 1013 245
Cuts at: 5909 736
Size: 1693
Fragments arranged by size:
1916 1693 1098 1013 360 314 245 142
85
NaeI GCC'GGC
Cuts at: 5047 5047
Size: 6866
NarI GG'CG_CC
Cuts at: 4297 4545 4297
Size: 248 6618
NcoI C'CATG_G
Cuts at: 1301 2729 1301
Size: 1428 5438
NdeI CA'TA_TG
Cuts at: 1605 1605
Size: 6866
NgoMIV G'CCGG_C
Cuts at: 5045 5045
Size: 6866
NspI r_CATG'y
Cuts at: 3478 3919 4419 5326 3478
Size: 441 500 907 5018
Fragments arranged by size:
5018 907 500 441
PasI CC'CwG_GG
Cuts at: 3154 3154
Size: 6866
PciI A'CATG_T
Cuts at: 3474 5322 3474
Size: 1848 5018
PflMI CCAn_nnn'nTGG
Cuts at: 612 2488 612
Size: 1876 4990
PmeI GTTT'AAAC
Cuts at: 3865 3865
Size: 6866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3072 6005 6037 3040
Size: 32 2933 32 3869
Fragments arranged by size:
3869 2933 32 32
PpuMI rG'GwC_Cy
Cuts at: 1371 1823 3228 1371
Size: 452 1405 5009
Fragments arranged by size:
5009 1405 452
PstI C_TGCA'G
Cuts at: 2906 3913 4598 2906
Size: 1007 685 5174
Fragments arranged by size:
5174 1007 685
PvuI CG_AT'CG
Cuts at: 104 318 104
Size: 214 6652
PvuII CAG'CTG
Cuts at: 736 4291 4651 736
Size: 3555 360 2951
Fragments arranged by size:
3555 2951 360
RsrII CG'GwC_CG
Cuts at: 5061 5061
Size: 6866
SacI G_AGCT'C
Cuts at: 3880 3880
Size: 6866
SacII CC_GC'GG
Cuts at: 3836 3836
Size: 6866
SalI G'TCGA_C
Cuts at: 3903 3903
Size: 6866
SapI GCTCTTCn'nnn_
Cuts at: 491 4889 5099 491
Size: 4398 210 2258
Fragments arranged by size:
4398 2258 210
SbfI CC_TGCA'GG
Cuts at: 3913 3913
Size: 6866
ScaI AGT'ACT
Cuts at: 6383 6383
Size: 6866
SexAI A'CCwGG_T
Cuts at: 584 2741 584
Size: 2157 4709
SfcI C'TryA_G
Cuts at: 33 177 2216 2236 2902 3909 4594 5587 5778
Size: 144 2039 20 666 1007 685 993 191
Cuts at: 5778 33
Size: 1121
Fragments arranged by size:
2039 1121 1007 993 685 666 191 144
20
SfoI GGC'GCC
Cuts at: 4298 4546 4298
Size: 248 6618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6866
SmaI CCC'GGG
Cuts at: 3888 3888
Size: 6866
SmlI C'TyrA_G
Cuts at: 2539 4021 4936 5428 5690 2539
Size: 1482 915 492 262 3715
Fragments arranged by size:
3715 1482 915 492 262
SpeI A'CTAG_T
Cuts at: 67 540 67
Size: 473 6393
SphI G_CATG'C
Cuts at: 3919 4419 3919
Size: 500 6366
StyI C'CwwG_G
Cuts at: 1301 2729 4000 4354 1301
Size: 1428 1271 354 3813
Fragments arranged by size:
3813 1428 1271 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2283 4449 4582 5041 5250 2283
Size: 2166 133 459 209 3899
Fragments arranged by size:
3899 2166 459 209 133
TatI w'GTAC_w
Cuts at: 112 292 410 1382 3080 3839 6381 112
Size: 180 118 972 1698 759 2542 597
Fragments arranged by size:
2542 1698 972 759 597 180 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 948 980 2418 2450 3661 3693 6383 6415 948
Size: 32 1438 32 1211 32 2690 32 1399
Fragments arranged by size:
2690 1438 1399 1211 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1352 4663 1352
Size: 3311 3555
XbaI T'CTAG_A
Cuts at: 3897 3897
Size: 6866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1848 2827 4361 1848
Size: 979 1534 4353
Fragments arranged by size:
4353 1534 979
XhoI C'TCGA_G
Cuts at: 2539 2539
Size: 6866
XmaI C'CCGG_G
Cuts at: 3886 3886
Size: 6866
XmnI GAAnn'nnTTC
Cuts at: 170 1337 2534 170
Size: 1167 1197 4502
Fragments arranged by size:
4502 1197 1167
ZraI GAC'GTC
Cuts at: 702 702
Size: 6866
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII ApaI AscI AvrII BbvCI BfrBI BmtI Bpu10I
BsiWI BssHII BstEII BstZ17I Bsu36I CspCI EcoNI FseI
FspAI MfeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI