Restriction map for
pF1KE2522
(Circular) (Six-base) MAPSORT of: pF1KE2522.seq Check: 5618 from: 1 to: 5885
pF1KE2522 5885 bp
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 435 435
Size: 5885
AccI GT'mk_AC
Cuts at: 1307 1748 2923 1307
Size: 441 1175 4269
Fragments arranged by size:
4269 1175 441
Acc65I G'GTAC_C
Cuts at: 2901 2901
Size: 5885
AclI AA'CG_TT
Cuts at: 5725 5725
Size: 5885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1002 3710 4142 4889 5157 665
Size: 337 2708 432 747 268 1393
Fragments arranged by size:
2708 1393 747 432 337 268
AflIII A'CryG_T
Cuts at: 3237 4341 5318 3237
Size: 1104 977 3804
Fragments arranged by size:
3804 1104 977
AgeI A'CCGG_T
Cuts at: 4273 4273
Size: 5885
AleI CACnn'nnGTG
Cuts at: 501 501
Size: 5885
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1441 1441
Size: 5885
AlwNI CAG_nnn'CTG
Cuts at: 681 1025 1482 1872 1965 2381 2565 2693 3190
Size: 344 457 390 93 416 184 128 497
Cuts at: 3190 4757 681
Size: 1567 1809
Fragments arranged by size:
1809 1567 497 457 416 390 344 184
128 93
ApaI G_GGCC'C
Cuts at: 173 326 173
Size: 153 5732
ApaLI G'TGCA_C
Cuts at: 2425 4655 2425
Size: 2230 3655
ApoI r'AATT_y
Cuts at: 2889 5238 5494 2889
Size: 2349 256 3280
Fragments arranged by size:
3280 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5885
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5885
AvaI C'yCGr_G
Cuts at: 1801 2364 2905 1801
Size: 563 541 4781
Fragments arranged by size:
4781 563 541
BamHI G'GATC_C
Cuts at: 451 929 2910 451
Size: 478 1981 3426
Fragments arranged by size:
3426 1981 478
BanI G'GyrC_C
Cuts at: 180 1393 2261 2294 2335 2901 3315 3563 3598
Size: 1213 868 33 41 566 414 248 35
Cuts at: 3598 180
Size: 2467
Fragments arranged by size:
2467 1213 868 566 414 248 41 35
33
BanII G_rGCy'C
Cuts at: 173 326 653 1084 1459 1919 2000 2458 2899
Size: 153 327 431 375 460 81 458 441
Cuts at: 2899 3929 173
Size: 1030 2129
Fragments arranged by size:
2129 1030 460 458 441 431 375 327
153 81
BbeI G_GCGC'C
Cuts at: 1397 2265 3319 3567 1397
Size: 868 1054 248 3715
Fragments arranged by size:
3715 1054 868 248
BbsI GAAGACnn'nnnn_
Cuts at: 747 1482 747
Size: 735 5150
BbvCI CC'TCA_GC
Cuts at: 909 1282 909
Size: 373 5512
BciVI GTATCCnnnnn_n'
Cuts at: 3783 4544 5885 3783
Size: 761 1341 3783
Fragments arranged by size:
3783 1341 761
BclI T'GATC_A
Cuts at: 3405 3405
Size: 5885
BglI GCCn_nnn'nGGC
Cuts at: 122 1928 2344 2410 122
Size: 1806 416 66 3597
Fragments arranged by size:
3597 1806 416 66
BglII A'GATC_T
Cuts at: 162 701 2448 3400 5437 162
Size: 539 1747 952 2037 610
Fragments arranged by size:
2037 1747 952 610 539
BlpI GC'TnA_GC
Cuts at: 1078 1453 2374 2997 1078
Size: 375 921 623 3966
Fragments arranged by size:
3966 921 623 375
Bme1580I G_kGCm'C
Cuts at: 173 185 326 508 2055 2276 2297 2429 3510
Size: 12 141 182 1547 221 21 132 1081
Cuts at: 3510 3603 4659 173
Size: 93 1056 1399
Fragments arranged by size:
1547 1399 1081 1056 221 182 141 132
93 21 12
BmgBI CAC'GTC
Cuts at: 1488 1761 1488
Size: 273 5612
BmrI ACTGGGnnnn_n'
Cuts at: 909 1035 2561 3097 3178 3513 5657 909
Size: 126 1526 536 81 335 2144 1137
Fragments arranged by size:
2144 1526 1137 536 335 126 81
BmtI G_CTAG'C
Cuts at: 1768 1768
Size: 5885
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1098 4252 1098
Size: 3154 2731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 766 921 2011 2077 2682 2742 589
Size: 177 155 1090 66 605 60 3732
Fragments arranged by size:
3732 1090 605 177 155 66 60
Bpu10I CC'TnA_GC
Cuts at: 909 1282 909
Size: 373 5512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3061 3940 4432 4730 3061
Size: 879 492 298 4216
Fragments arranged by size:
4216 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1986 5589 1986
Size: 3603 2282
BsaAI yAC'GTr
Cuts at: 1503 1779 3238 1503
Size: 276 1459 4150
Fragments arranged by size:
4150 1459 276
BsaBI GATnn'nnATC
Cuts at: 6 253 3399 5436 6
Size: 247 3146 2037 455
Fragments arranged by size:
3146 2037 455 247
BsaHI Gr'CG_yC
Cuts at: 1394 2262 3316 3564 4266 5762 1394
Size: 868 1054 248 702 1496 1517
Fragments arranged by size:
1517 1496 1054 868 702 248
BsaWI w'CCGG_w
Cuts at: 1836 3074 3595 4273 4547 4694 5105 1836
Size: 1238 521 678 274 147 411 2616
Fragments arranged by size:
2616 1238 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 474 504 1987 2017 474
Size: 30 1483 30 4342
Fragments arranged by size:
4342 1483 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 200 203 206 288 631 946 1087 1927
Size: 22 3 3 82 343 315 141 840
Cuts at: 1927 2002 2008 2743 178
Size: 75 6 735 3320
Fragments arranged by size:
3320 840 735 343 315 141 82 75
22 6 3 3
BseYI C'CCAG_C
Cuts at: 125 147 387 762 1352 1409 1533 2098 2527
Size: 22 240 375 590 57 124 565 429
Cuts at: 2527 2570 3310 4068 4645 5141 125
Size: 43 740 758 577 496 869
Fragments arranged by size:
869 758 740 590 577 565 496 429
375 240 124 57 43 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1123 1168 1468 2367 2605 1042
Size: 81 45 300 899 238 4322
Fragments arranged by size:
4322 899 300 238 81 45
BsiEI CG_ry'CG
Cuts at: 104 3427 3473 4681 104
Size: 3323 46 1208 1308
Fragments arranged by size:
3323 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 378 1084 1742 1778 1784 2000 2375 2429 2899
Size: 706 658 36 6 216 375 54 470
Cuts at: 2899 3677 3867 4659 378
Size: 778 190 792 1604
Fragments arranged by size:
1604 792 778 706 658 470 375 216
190 54 36 6
Bsp1286I G_dGCh'C
Cuts at: 173 185 326 378 508 653 1084 1459 1742
Size: 12 141 52 130 145 431 375 283
Cuts at: 1742 1778 1784 1919 2000 2055 2276 2297 2375
Size: 36 6 135 81 55 221 21 78
Cuts at: 2375 2429 2458 2899 3510 3603 3677 3867 3929
Size: 54 29 441 611 93 74 190 62
Cuts at: 3929 4659 173
Size: 730 1399
Fragments arranged by size:
1399 730 611 441 431 375 283 221
190 145 141 135 130 93 81 78
74 62 55 54 52 36 29 21
12 6
BspEI T'CCGG_A
Cuts at: 1836 5105 1836
Size: 3269 2616
BspHI T'CATG_A
Cuts at: 5061 5061
Size: 5885
BspMI ACCTGCnnnn'nnnn_
Cuts at: 423 435 1319 1467 2627 2935 3149 3451 3832
Size: 12 884 148 1160 308 214 302 381
Cuts at: 3832 423
Size: 2476
Fragments arranged by size:
2476 1160 884 381 308 302 214 148
12
BsrBI CCG'CTC
Cuts at: 50 564 935 1133 1978 2010 4177 4231 5715
Size: 514 371 198 845 32 2167 54 1484
Cuts at: 5715 5880 50
Size: 165 55
Fragments arranged by size:
2167 1484 845 514 371 198 165 55
54 32
BsrDI GCAATG_nn'
Cuts at: 425 3797 425
Size: 3372 2513
BsrFI r'CCGG_y
Cuts at: 973 1239 1946 3883 4064 4273 973
Size: 266 707 1937 181 209 2585
Fragments arranged by size:
2585 1937 707 266 209 181
BssHII G'CGCG_C
Cuts at: 1405 1529 1540 1561 1605 1607 1609 1405
Size: 124 11 21 44 2 2 5681
Fragments arranged by size:
5681 124 44 21 11 2 2
BssSI C'ACGA_G
Cuts at: 1597 2048 4156 4514 1597
Size: 451 2108 358 2968
Fragments arranged by size:
2968 2108 451 358
BstAPI GCAn_nnn'nTGC
Cuts at: 630 1482 1602 1872 1965 3262 630
Size: 852 120 270 93 1297 3253
Fragments arranged by size:
3253 1297 852 270 120 93
BstBI TT'CG_AA
Cuts at: 4246 4246
Size: 5885
BstEII G'GTnAC_C
Cuts at: 1806 2832 1806
Size: 1026 4859
BstXI CCAn_nnnn'nTGG
Cuts at: 724 724
Size: 5885
BstYI r'GATC_y
Cuts at: 1 162 248 451 701 929 2448 2910 3377
Size: 161 86 203 250 228 1519 462 467
Cuts at: 3377 3400 3735 3981 4993 5079 5091 5437 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1519 1012 467 462 449 346 335 250
246 228 203 161 86 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 584 2399 584
Size: 1815 4070
BtgI C'CryG_G
Cuts at: 107 609 1153 1287 1323 1344 1769 2145 2656
Size: 502 544 134 36 21 425 376 511
Cuts at: 2656 107
Size: 3336
Fragments arranged by size:
3336 544 511 502 425 376 134 36
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3835 4016 4179 3835
Size: 181 163 5541
Fragments arranged by size:
5541 181 163
BtsI GCAGTG_nn'
Cuts at: 199 508 1120 1275 1852 3128 199
Size: 309 612 155 577 1276 2956
Fragments arranged by size:
2956 1276 612 577 309 155
DraI TTT'AAA
Cuts at: 2884 2884
Size: 5885
DrdI GACnn_nn'nnGTC
Cuts at: 3591 4449 3591
Size: 858 5027
EaeI y'GGCC_r
Cuts at: 1220 1433 2411 2480 3470 3644 4035 4062 5407
Size: 213 978 69 990 174 391 27 1345
Cuts at: 5407 1220
Size: 1698
Fragments arranged by size:
1698 1345 990 978 391 213 174 69
27
EagI C'GGCC_G
Cuts at: 3470 3470
Size: 5885
EarI CTCTTCn'nnn_
Cuts at: 1021 1324 1345 1726 1835 2425 3908 4118 1021
Size: 303 21 381 109 590 1483 210 2788
Fragments arranged by size:
2788 1483 590 381 303 210 109 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 4093 4403 4549 5695 2032
Size: 2061 310 146 1146 2222
Fragments arranged by size:
2222 2061 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1082 1998 2897 1082
Size: 916 899 4070
Fragments arranged by size:
4070 916 899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 665 766 921 1002 2011 2077 2682 2742
Size: 76 101 155 81 1009 66 605 60
Cuts at: 2742 3710 4142 4889 5157 589
Size: 968 432 747 268 1317
Fragments arranged by size:
1317 1009 968 747 605 432 268 155
101 81 76 66 60
EcoNI CCTnn'n_nnAGG
Cuts at: 239 1909 1990 2398 239
Size: 1670 81 408 3726
Fragments arranged by size:
3726 1670 408 81
EcoO109I rG'GnC_Cy
Cuts at: 169 232 322 323 535 590 1053 1137 2523
Size: 63 90 1 212 55 463 84 1386
Cuts at: 2523 2690 3024 169
Size: 167 334 3030
Fragments arranged by size:
3030 1386 463 334 212 167 90 84
63 55 1
EcoRI G'AATT_C
Cuts at: 2889 2889
Size: 5885
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3904 3904
Size: 5885
FspI TGC'GCA
Cuts at: 1651 1678 3666 1651
Size: 27 1988 3870
Fragments arranged by size:
3870 1988 27
HaeII r_GCGC'y
Cuts at: 1219 1300 1397 1453 2145 2265 3319 3567 4589
Size: 81 97 56 692 120 1054 248 1022
Cuts at: 4589 5568 1219
Size: 979 1536
Fragments arranged by size:
1536 1054 1022 979 692 248 120 97
81 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 233 265 1090 1122 1987 2019 2515 2547 2722
Size: 32 825 32 865 32 496 32 175
Cuts at: 2722 2754 2851 2883 3727 3757 3759 3789 233
Size: 32 97 32 844 30 2 30 2329
Fragments arranged by size:
2329 865 844 825 496 175 97 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1308 2924 1308
Size: 1616 4269
KasI G'GCGC_C
Cuts at: 1393 2261 3315 3563 1393
Size: 868 1054 248 3715
Fragments arranged by size:
3715 1054 868 248
KpnI G_GTAC'C
Cuts at: 2905 2905
Size: 5885
MluI A'CGCG_T
Cuts at: 5318 5318
Size: 5885
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1014 4556 4740 1014
Size: 3542 184 2159
Fragments arranged by size:
3542 2159 184
MscI TGG'CCA
Cuts at: 1222 2413 3646 1222
Size: 1191 1233 3461
Fragments arranged by size:
3461 1233 1191
MslI CAynn'nnrTG
Cuts at: 501 1036 1789 3874 4001 501
Size: 535 753 2085 127 2385
Fragments arranged by size:
2385 2085 753 535 127
MspA1I CmG'CkG
Cuts at: 720 762 945 1128 1155 1247 1254 1289 1325
Size: 42 183 183 27 92 7 35 36
Cuts at: 1325 1346 1389 1461 1572 1657 1962 2022 2326
Size: 21 43 72 111 85 305 60 304
Cuts at: 2326 2476 2996 3310 3670 4683 4928 720
Size: 150 520 314 360 1013 245 1677
Fragments arranged by size:
1677 1013 520 360 314 305 304 245
183 183 150 111 92 85 72 60
43 42 36 35 27 21 7
NaeI GCC'GGC
Cuts at: 975 1241 4066 975
Size: 266 2825 2794
Fragments arranged by size:
2825 2794 266
NarI GG'CG_CC
Cuts at: 1394 2262 3316 3564 1394
Size: 868 1054 248 3715
Fragments arranged by size:
3715 1054 868 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5885
NgoMIV G'CCGG_C
Cuts at: 973 1239 4064 973
Size: 266 2825 2794
Fragments arranged by size:
2825 2794 266
NheI G'CTAG_C
Cuts at: 1764 1764
Size: 5885
NspI r_CATG'y
Cuts at: 1899 2938 3438 4345 1899
Size: 1039 500 907 3439
Fragments arranged by size:
3439 1039 907 500
PasI CC'CwG_GG
Cuts at: 1091 1259 2269 1091
Size: 168 1010 4707
Fragments arranged by size:
4707 1010 168
PciI A'CATG_T
Cuts at: 4341 4341
Size: 5885
PflMI CCAn_nnn'nTGG
Cuts at: 681 2565 681
Size: 1884 4001
PfoI T'CCnGG_A
Cuts at: 1907 1988 1907
Size: 81 5804
PmeI GTTT'AAAC
Cuts at: 2884 2884
Size: 5885
PmlI CAC'GTG
Cuts at: 1503 1779 1503
Size: 276 5609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5024 5056 5024
Size: 32 5853
PpuMI rG'GwC_Cy
Cuts at: 232 590 2523 232
Size: 358 1933 3594
Fragments arranged by size:
3594 1933 358
PspOMI G'GGCC_C
Cuts at: 169 322 169
Size: 153 5732
PstI C_TGCA'G
Cuts at: 1127 1880 1970 2673 2932 3617 1127
Size: 753 90 703 259 685 3395
Fragments arranged by size:
3395 753 703 685 259 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5885
PvuII CAG'CTG
Cuts at: 762 1572 1962 2326 2476 3310 3670 762
Size: 810 390 364 150 834 360 2977
Fragments arranged by size:
2977 834 810 390 364 360 150
RsrII CG'GwC_CG
Cuts at: 4080 4080
Size: 5885
SacI G_AGCT'C
Cuts at: 1084 2000 2899 1084
Size: 916 899 4070
Fragments arranged by size:
4070 916 899
SacII CC_GC'GG
Cuts at: 1156 1290 1326 1347 1156
Size: 134 36 21 5694
Fragments arranged by size:
5694 134 36 21
SalI G'TCGA_C
Cuts at: 1306 2922 1306
Size: 1616 4269
SanDI GG'GwC_CC
Cuts at: 590 590
Size: 5885
SapI GCTCTTCn'nnn_
Cuts at: 1324 1345 1835 3908 4118 1324
Size: 21 490 2073 210 3091
Fragments arranged by size:
3091 2073 490 210 21
SbfI CC_TGCA'GG
Cuts at: 2932 2932
Size: 5885
ScaI AGT'ACT
Cuts at: 5402 5402
Size: 5885
SexAI A'CCwGG_T
Cuts at: 1208 1208
Size: 5885
SfcI C'TryA_G
Cuts at: 33 340 705 1123 1494 1876 1966 2669 2928
Size: 307 365 418 371 382 90 703 259
Cuts at: 2928 3613 4606 4797 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 703 685 418 382 371 365
307 259 191 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 2410 2410
Size: 5885
SfoI GGC'GCC
Cuts at: 1395 2263 3317 3565 1395
Size: 868 1054 248 3715
Fragments arranged by size:
3715 1054 868 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5885
SmaI CCC'GGG
Cuts at: 2366 2907 2366
Size: 541 5344
SmlI C'TyrA_G
Cuts at: 3040 3955 4447 4709 3040
Size: 915 492 262 4216
Fragments arranged by size:
4216 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5885
SphI G_CATG'C
Cuts at: 2938 3438 2938
Size: 500 5385
StuI AGG'CCT
Cuts at: 1232 2185 2404 1232
Size: 953 219 4713
Fragments arranged by size:
4713 953 219
StyI C'CwwG_G
Cuts at: 107 3019 3373 107
Size: 2912 354 2619
Fragments arranged by size:
2912 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 618 2860 3468 3601 4060 4269 618
Size: 2242 608 133 459 209 2234
Fragments arranged by size:
2242 2234 608 459 209 133
TatI w'GTAC_w
Cuts at: 2310 5400 2310
Size: 3090 2795
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1831 1863 5402 5434 1831
Size: 32 3539 32 2282
Fragments arranged by size:
3539 2282 32 32
Tth111I GACn'n_nGTC
Cuts at: 888 3682 888
Size: 2794 3091
XbaI T'CTAG_A
Cuts at: 2916 2916
Size: 5885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 517 721 859 945 1209 3380 468
Size: 49 204 138 86 264 2171 2973
Fragments arranged by size:
2973 2171 264 204 138 86 49
XmaI C'CCGG_G
Cuts at: 2364 2905 2364
Size: 541 5344
XmnI GAAnn'nnTTC
Cuts at: 2812 2812
Size: 5885
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BfrBI BsiWI BsmI BsmBI BsrGI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI