Restriction map for
pF1KE2521
(Circular) (Six-base) MAPSORT of: pF1KE2521.seq Check: 9129 from: 1 to: 4766
pF1KE2521 4766 bp
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AccI GT'mk_AC
Cuts at: 1804 1804
Size: 4766
Acc65I G'GTAC_C
Cuts at: 1782 1782
Size: 4766
AclI AA'CG_TT
Cuts at: 4606 4606
Size: 4766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2591 3023 3770 4038 2591
Size: 432 747 268 3319
Fragments arranged by size:
3319 747 432 268
AflIII A'CryG_T
Cuts at: 2118 3222 4199 2118
Size: 1104 977 2685
Fragments arranged by size:
2685 1104 977
AgeI A'CCGG_T
Cuts at: 1341 3154 1341
Size: 1813 2953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 335 367 335
Size: 32 4734
AlwNI CAG_nnn'CTG
Cuts at: 2071 3638 2071
Size: 1567 3199
ApaLI G'TGCA_C
Cuts at: 3536 3536
Size: 4766
ApoI r'AATT_y
Cuts at: 1254 1611 1770 4119 4375 1254
Size: 357 159 2349 256 1645
Fragments arranged by size:
2349 1645 357 256 159
AseI AT'TA_AT
Cuts at: 20 20
Size: 4766
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 1786 1786
Size: 4766
BamHI G'GATC_C
Cuts at: 1704 1791 1704
Size: 87 4679
BanI G'GyrC_C
Cuts at: 796 810 1782 2196 2444 2479 796
Size: 14 972 414 248 35 3083
Fragments arranged by size:
3083 972 414 248 35 14
BanII G_rGCy'C
Cuts at: 1219 1780 2810 1219
Size: 561 1030 3175
Fragments arranged by size:
3175 1030 561
BbeI G_GCGC'C
Cuts at: 2200 2448 2200
Size: 248 4518
BbsI GAAGACnn'nnnn_
Cuts at: 141 212 467 667 677 1385 141
Size: 71 255 200 10 708 3522
Fragments arranged by size:
3522 708 255 200 71 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1158 1192 1158
Size: 34 4732
BciVI GTATCCnnnnn_n'
Cuts at: 361 2664 3425 4766 361
Size: 2303 761 1341 361
Fragments arranged by size:
2303 1341 761 361
BclI T'GATC_A
Cuts at: 2286 2286
Size: 4766
BglII A'GATC_T
Cuts at: 378 472 2281 4318 378
Size: 94 1809 2037 826
Fragments arranged by size:
2037 1809 826 94
BlpI GC'TnA_GC
Cuts at: 119 1213 1433 1878 119
Size: 1094 220 445 3007
Fragments arranged by size:
3007 1094 445 220
Bme1580I G_kGCm'C
Cuts at: 2391 2484 3540 2391
Size: 93 1056 3617
Fragments arranged by size:
3617 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 997 1978 2059 2394 4538 997
Size: 981 81 335 2144 1225
Fragments arranged by size:
2144 1225 981 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 873 3133 873
Size: 2260 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 686 1304 1709 124
Size: 562 618 405 3181
Fragments arranged by size:
3181 618 562 405
Bpu10I CC'TnA_GC
Cuts at: 1644 1644
Size: 4766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 382 1942 2821 3313 3611 264
Size: 118 1560 879 492 298 1419
Fragments arranged by size:
1560 1419 879 492 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 4470 4470
Size: 4766
BsaAI yAC'GTr
Cuts at: 2119 2119
Size: 4766
BsaBI GATnn'nnATC
Cuts at: 6 2280 4317 6
Size: 2274 2037 455
Fragments arranged by size:
2274 2037 455
BsaHI Gr'CG_yC
Cuts at: 2197 2445 3147 4643 2197
Size: 248 702 1496 2320
Fragments arranged by size:
2320 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1341 1955 2476 3154 3428 3575 3986 1341
Size: 614 521 678 274 147 411 2121
Fragments arranged by size:
2121 678 614 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1471 1474 1477 1471
Size: 3 3 4760
Fragments arranged by size:
4760 3 3
BseYI C'CCAG_C
Cuts at: 1743 2191 2949 3526 4022 1743
Size: 448 758 577 496 2487
Fragments arranged by size:
2487 758 577 496 448
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 803
Size: 4766
BsiEI CG_ry'CG
Cuts at: 104 2308 2354 3562 104
Size: 2204 46 1208 1308
Fragments arranged by size:
2204 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1780 2558 2748 3540 1780
Size: 778 190 792 3006
Fragments arranged by size:
3006 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1219 1780 2391 2484 2558 2748 2810 3540 1219
Size: 561 611 93 74 190 62 730 2445
Fragments arranged by size:
2445 730 611 561 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3986 3986
Size: 4766
BspHI T'CATG_A
Cuts at: 879 1578 3942 879
Size: 699 2364 1703
Fragments arranged by size:
2364 1703 699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1816 2030 2332 2713 1816
Size: 214 302 381 3869
Fragments arranged by size:
3869 381 302 214
BsrBI CCG'CTC
Cuts at: 50 450 3058 3112 4596 4761 50
Size: 400 2608 54 1484 165 55
Fragments arranged by size:
2608 1484 400 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1032 2678 1032
Size: 1646 3120
BsrFI r'CCGG_y
Cuts at: 1341 2764 2945 3154 1341
Size: 1423 181 209 2953
Fragments arranged by size:
2953 1423 209 181
BssSI C'ACGA_G
Cuts at: 3037 3395 3037
Size: 358 4408
BstAPI GCAn_nnn'nTGC
Cuts at: 2143 2143
Size: 4766
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstEII G'GTnAC_C
Cuts at: 936 936
Size: 4766
BstYI r'GATC_y
Cuts at: 1 329 371 378 472 1704 1791 2258 2281
Size: 328 42 7 94 1232 87 467 23
Cuts at: 2281 2616 2862 3874 3960 3972 4318 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1232 1012 467 449 346 335 328 246
94 87 86 42 23 12 7
Bsu36I CC'TnA_GG
Cuts at: 355 355
Size: 4766
BtgI C'CryG_G
Cuts at: 107 632 107
Size: 525 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2716 2897 3060 2716
Size: 181 163 4422
Fragments arranged by size:
4422 181 163
BtsI GCAGTG_nn'
Cuts at: 2009 2009
Size: 4766
DraI TTT'AAA
Cuts at: 428 714 1765 428
Size: 286 1051 3429
Fragments arranged by size:
3429 1051 286
DrdI GACnn_nn'nnGTC
Cuts at: 2472 3330 2472
Size: 858 3908
EaeI y'GGCC_r
Cuts at: 2351 2525 2916 2943 4288 2351
Size: 174 391 27 1345 2829
Fragments arranged by size:
2829 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2351 2351
Size: 4766
EarI CTCTTCn'nnn_
Cuts at: 287 410 500 1112 2789 2999 287
Size: 123 90 612 1677 210 2054
Fragments arranged by size:
2054 1677 612 210 123 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1067 2974 3284 3430 4576 1067
Size: 1907 310 146 1146 1257
Fragments arranged by size:
1907 1257 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1778 1778
Size: 4766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 686 1304 1709 2591 3023 3770 4038 124
Size: 562 618 405 882 432 747 268 852
Fragments arranged by size:
882 852 747 618 562 432 405 268
EcoNI CCTnn'n_nnAGG
Cuts at: 906 906
Size: 4766
EcoO109I rG'GnC_Cy
Cuts at: 1905 1905
Size: 4766
EcoRI G'AATT_C
Cuts at: 1254 1770 1254
Size: 516 4250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1141 2785 1141
Size: 1644 3122
FspI TGC'GCA
Cuts at: 2547 2547
Size: 4766
HaeII r_GCGC'y
Cuts at: 2200 2448 3470 4449 2200
Size: 248 1022 979 2517
Fragments arranged by size:
2517 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 363 395 1180 1212 1286 1318 1474 1506 1689
Size: 32 785 32 74 32 156 32 183
Cuts at: 1689 1693 1721 1725 2608 2638 2640 2670 363
Size: 4 28 4 883 30 2 30 2459
Fragments arranged by size:
2459 883 785 183 156 74 32 32
32 32 30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 1805 1805
Size: 4766
HindIII A'AGCT_T
Cuts at: 620 1123 1641 620
Size: 503 518 3745
Fragments arranged by size:
3745 518 503
KasI G'GCGC_C
Cuts at: 2196 2444 2196
Size: 248 4518
KpnI G_GTAC'C
Cuts at: 1786 1786
Size: 4766
MfeI C'AATT_G
Cuts at: 443 512 443
Size: 69 4697
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3437 3621 3437
Size: 184 4582
MscI TGG'CCA
Cuts at: 2527 2527
Size: 4766
MslI CAynn'nnrTG
Cuts at: 2755 2882 2755
Size: 127 4639
MspA1I CmG'CkG
Cuts at: 278 1877 2191 2551 3564 3809 278
Size: 1599 314 360 1013 245 1235
Fragments arranged by size:
1599 1235 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 4766
NarI GG'CG_CC
Cuts at: 2197 2445 2197
Size: 248 4518
NcoI C'CATG_G
Cuts at: 107 632 107
Size: 525 4241
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 4766
NspI r_CATG'y
Cuts at: 1819 2319 3226 1819
Size: 500 907 3359
Fragments arranged by size:
3359 907 500
PciI A'CATG_T
Cuts at: 3222 3222
Size: 4766
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4734
PsiI TTA'TAA
Cuts at: 469 469
Size: 4766
PstI C_TGCA'G
Cuts at: 1813 2498 1813
Size: 685 4081
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 2191 2551 2191
Size: 360 4406
RsrII CG'GwC_CG
Cuts at: 2961 2961
Size: 4766
SacI G_AGCT'C
Cuts at: 1780 1780
Size: 4766
SalI G'TCGA_C
Cuts at: 1803 1803
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 2789 2999 2789
Size: 210 4556
SbfI CC_TGCA'GG
Cuts at: 1813 1813
Size: 4766
ScaI AGT'ACT
Cuts at: 4283 4283
Size: 4766
SfcI C'TryA_G
Cuts at: 33 1809 2494 3487 3678 33
Size: 1776 685 993 191 1121
Fragments arranged by size:
1776 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2198 2446 2198
Size: 248 4518
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1788 1788
Size: 4766
SmlI C'TyrA_G
Cuts at: 243 361 1921 2836 3328 3590 243
Size: 118 1560 915 492 262 1419
Fragments arranged by size:
1560 1419 915 492 262 118
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 1819 2319 1819
Size: 500 4266
SspI AAT'ATT
Cuts at: 1232 1232
Size: 4766
StuI AGG'CCT
Cuts at: 241 912 241
Size: 671 4095
StyI C'CwwG_G
Cuts at: 107 632 1156 1207 1900 2254 107
Size: 525 524 51 693 354 2619
Fragments arranged by size:
2619 693 525 524 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 2349 2482 2941 3150 1278
Size: 1071 133 459 209 2894
Fragments arranged by size:
2894 1071 459 209 133
TatI w'GTAC_w
Cuts at: 673 4281 673
Size: 3608 1158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1597 1629 1739 1771 4283 4315 1597
Size: 32 110 32 2512 32 2048
Fragments arranged by size:
2512 2048 110 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2563 2563
Size: 4766
XbaI T'CTAG_A
Cuts at: 261 339 1797 261
Size: 78 1458 3230
Fragments arranged by size:
3230 1458 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1017 2261 1017
Size: 1244 3522
XmaI C'CCGG_G
Cuts at: 1786 1786
Size: 4766
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI