Restriction map for
pF1KE2510
(Circular) (Six-base) MAPSORT of: pF1KE2510.seq Check: 5881 from: 1 to: 6491
pF1KE2510 6491 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 354 1827 2082 2343 2406 2629 3174 354
Size: 1473 255 261 63 223 545 3671
Fragments arranged by size:
3671 1473 545 261 255 223 63
AatII G_ACGT'C
Cuts at: 1631 1631
Size: 6491
AccI GT'mk_AC
Cuts at: 697 3529 697
Size: 2832 3659
Acc65I G'GTAC_C
Cuts at: 3507 3507
Size: 6491
AclI AA'CG_TT
Cuts at: 275 6331 275
Size: 6056 435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1955 4316 4748 5495 5763 889
Size: 1066 2361 432 747 268 1617
Fragments arranged by size:
2361 1617 1066 747 432 268
AflIII A'CryG_T
Cuts at: 3843 4947 5924 3843
Size: 1104 977 4410
Fragments arranged by size:
4410 1104 977
AgeI A'CCGG_T
Cuts at: 440 4879 440
Size: 4439 2052
AleI CACnn'nnGTG
Cuts at: 1621 2501 1621
Size: 880 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1000 1695 1000
Size: 695 5796
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1618 1650 1618
Size: 32 6459
AlwNI CAG_nnn'CTG
Cuts at: 300 519 543 1011 1101 1354 1401 1877 3796
Size: 219 24 468 90 253 47 476 1919
Cuts at: 3796 5363 300
Size: 1567 1428
Fragments arranged by size:
1919 1567 1428 476 468 253 219 90
47 24
ApaI G_GGCC'C
Cuts at: 185 326 1909 2221 2794 2848 3152 3307 185
Size: 141 1583 312 573 54 304 155 3369
Fragments arranged by size:
3369 1583 573 312 304 155 141 54
ApaLI G'TGCA_C
Cuts at: 1381 5261 1381
Size: 3880 2611
ApoI r'AATT_y
Cuts at: 3495 5844 6100 3495
Size: 2349 256 3886
Fragments arranged by size:
3886 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6491
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6491
AvaI C'yCGr_G
Cuts at: 224 493 2499 2514 2758 3511 224
Size: 269 2006 15 244 753 3204
Fragments arranged by size:
3204 2006 753 269 244 15
BamHI G'GATC_C
Cuts at: 1214 3516 1214
Size: 2302 4189
BanI G'GyrC_C
Cuts at: 499 522 708 1442 1512 1948 2284 2389 2503
Size: 23 186 734 70 436 336 105 114
Cuts at: 2503 3104 3258 3288 3507 3921 4169 4204 499
Size: 601 154 30 219 414 248 35 2786
Fragments arranged by size:
2786 734 601 436 414 336 248 219
186 154 114 105 70 35 30 23
BanII G_rGCy'C
Cuts at: 154 185 326 1793 1909 2214 2221 2349 2453
Size: 31 141 1467 116 305 7 128 104
Cuts at: 2453 2794 2839 2848 3125 3152 3307 3368 3505
Size: 341 45 9 277 27 155 61 137
Cuts at: 3505 4535 154
Size: 1030 2110
Fragments arranged by size:
2110 1467 1030 341 305 277 155 141
137 128 116 104 61 45 31 27
9 7
BbeI G_GCGC'C
Cuts at: 712 3108 3925 4173 712
Size: 2396 817 248 3030
Fragments arranged by size:
3030 2396 817 248
BbsI GAAGACnn'nnnn_
Cuts at: 1163 1888 1163
Size: 725 5766
BbvCI CC'TCA_GC
Cuts at: 933 1069 1438 2079 2563 933
Size: 136 369 641 484 4861
Fragments arranged by size:
4861 641 484 369 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1287 1321 3082 3116 3464 3498 1287
Size: 34 1761 34 348 34 4280
Fragments arranged by size:
4280 1761 348 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4389 5150 6491 4389
Size: 761 1341 4389
Fragments arranged by size:
4389 1341 761
BclI T'GATC_A
Cuts at: 686 4011 686
Size: 3325 3166
BfrBI ATG'CAT
Cuts at: 1485 1485
Size: 6491
BglI GCCn_nnn'nGGC
Cuts at: 112 137 1456 2044 112
Size: 25 1319 588 4559
Fragments arranged by size:
4559 1319 588 25
BglII A'GATC_T
Cuts at: 2002 4006 6043 2002
Size: 2004 2037 2450
Fragments arranged by size:
2450 2037 2004
BlpI GC'TnA_GC
Cuts at: 2339 2348 3031 3603 2339
Size: 9 683 572 5227
Fragments arranged by size:
5227 683 572 9
Bme1580I G_kGCm'C
Cuts at: 185 326 1094 1385 1515 1909 1951 1959 2221
Size: 141 768 291 130 394 42 8 262
Cuts at: 2221 2289 2392 2628 2794 2848 3152 3261 3307
Size: 68 103 236 166 54 304 109 46
Cuts at: 3307 4116 4209 5265 185
Size: 809 93 1056 1411
Fragments arranged by size:
1411 1056 809 768 394 304 291 262
236 166 141 130 109 103 93 68
54 46 42 8
BmrI ACTGGGnnnn_n'
Cuts at: 179 818 1904 1906 2670 2804 2818 3223 3703
Size: 639 1086 2 764 134 14 405 480
Cuts at: 3703 3784 4119 6263 179
Size: 81 335 2144 407
Fragments arranged by size:
2144 1086 764 639 480 407 405 335
134 81 14 2
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 4858 2855
Size: 2003 4488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 356 565 1447 1561 1762 2455 2850 2955
Size: 63 209 882 114 201 693 395 105
Cuts at: 2955 293
Size: 3829
Fragments arranged by size:
3829 882 693 395 209 201 114 105
63
Bpu10I CC'TnA_GC
Cuts at: 933 1069 1438 2079 2563 2632 3119 3311 933
Size: 136 369 641 484 69 487 192 4113
Fragments arranged by size:
4113 641 487 484 369 192 136 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2574 3667 4546 5038 5336 2574
Size: 1093 879 492 298 3729
Fragments arranged by size:
3729 1093 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6195 6195
Size: 6491
BsaAI yAC'GTr
Cuts at: 3844 3844
Size: 6491
BsaBI GATnn'nnATC
Cuts at: 6 4005 6042 6
Size: 3999 2037 455
Fragments arranged by size:
3999 2037 455
BsaHI Gr'CG_yC
Cuts at: 649 709 1628 1780 2174 3105 3922 4170 4872
Size: 60 919 152 394 931 817 248 702
Cuts at: 4872 6368 649
Size: 1496 772
Fragments arranged by size:
1496 931 919 817 772 702 394 248
152 60
BsaWI w'CCGG_w
Cuts at: 398 440 455 474 3680 4201 4879 5153 5300
Size: 42 15 19 3206 521 678 274 147
Cuts at: 5300 5711 398
Size: 411 1178
Fragments arranged by size:
3206 1178 678 521 411 274 147 42
19 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 728 758 1528 1558 2011 2041 2990
Size: 30 160 30 770 30 453 30 949
Cuts at: 2990 3020 538
Size: 30 4009
Fragments arranged by size:
4009 949 770 453 160 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 212 625 658 1375 1378 2032 2165 2580 2847
Size: 413 33 717 3 654 133 415 267
Cuts at: 2847 3168 3171 212
Size: 321 3 3532
Fragments arranged by size:
3532 717 654 415 413 321 267 133
33 3 3
BseYI C'CCAG_C
Cuts at: 175 325 1773 1941 2055 2062 2310 2627 2727
Size: 150 1448 168 114 7 248 317 100
Cuts at: 2727 2817 3089 3442 3916 4674 5251 5747 175
Size: 90 272 353 474 758 577 496 919
Fragments arranged by size:
1448 919 758 577 496 474 353 317
272 248 168 150 114 100 90 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 382 1609 2214 2837 178
Size: 204 1227 605 623 3832
Fragments arranged by size:
3832 1227 623 605 204
BsiEI CG_ry'CG
Cuts at: 104 624 677 2112 4033 4079 5287 104
Size: 520 53 1435 1921 46 1208 1308
Fragments arranged by size:
1921 1435 1308 1208 520 53 46
BsiHKAI G_wGCw'C
Cuts at: 596 1067 1385 2073 2839 3368 3505 4283 4473
Size: 471 318 688 766 529 137 778 190
Cuts at: 4473 5265 596
Size: 792 1822
Fragments arranged by size:
1822 792 778 766 688 529 471 318
190 137
BsmI GAATG_Cn'
Cuts at: 1237 1237
Size: 6491
BsmBI CGTCTCn'nnnn_
Cuts at: 929 929
Size: 6491
Bsp1286I G_dGCh'C
Cuts at: 154 185 326 596 1067 1094 1385 1515 1793
Size: 31 141 270 471 27 291 130 278
Cuts at: 1793 1909 1951 1959 2073 2214 2221 2289 2349
Size: 116 42 8 114 141 7 68 60
Cuts at: 2349 2392 2453 2628 2794 2839 2848 3125 3152
Size: 43 61 175 166 45 9 277 27
Cuts at: 3152 3261 3307 3368 3505 4116 4209 4283 4473
Size: 109 46 61 137 611 93 74 190
Cuts at: 4473 4535 5265 154
Size: 62 730 1380
Fragments arranged by size:
1380 730 611 471 291 278 277 270
190 175 166 141 141 137 130 116
114 109 93 74 68 62 61 61
60 46 45 43 42 31 27 27
9 8 7
BspEI T'CCGG_A
Cuts at: 398 474 5711 398
Size: 76 5237 1178
Fragments arranged by size:
5237 1178 76
BspHI T'CATG_A
Cuts at: 5667 5667
Size: 6491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 354 1442 1529 1827 2082 2343 2406 2629 3174
Size: 1088 87 298 255 261 63 223 545
Cuts at: 3174 3541 3755 4057 4438 354
Size: 367 214 302 381 2407
Fragments arranged by size:
2407 1088 545 381 367 302 298 261
255 223 214 87 63
BsrBI CCG'CTC
Cuts at: 50 852 2343 2423 2930 4783 4837 6321 6486
Size: 802 1491 80 507 1853 54 1484 165
Cuts at: 6486 50
Size: 55
Fragments arranged by size:
1853 1491 1484 802 507 165 80 55
54
BsrDI GCAATG_nn'
Cuts at: 4403 4403
Size: 6491
BsrFI r'CCGG_y
Cuts at: 390 440 623 964 1154 1657 1769 4489 4670
Size: 50 183 341 190 503 112 2720 181
Cuts at: 4670 4879 390
Size: 209 2002
Fragments arranged by size:
2720 2002 503 341 209 190 183 181
112 50
BsrGI T'GTAC_A
Cuts at: 3383 3383
Size: 6491
BssSI C'ACGA_G
Cuts at: 950 1195 4762 5120 950
Size: 245 3567 358 2321
Fragments arranged by size:
3567 2321 358 245
BstAPI GCAn_nnn'nTGC
Cuts at: 1011 3868 1011
Size: 2857 3634
BstBI TT'CG_AA
Cuts at: 4852 4852
Size: 6491
BstEII G'GTnAC_C
Cuts at: 366 366
Size: 6491
BstXI CCAn_nnnn'nTGG
Cuts at: 1324 1539 1324
Size: 215 6276
BstYI r'GATC_y
Cuts at: 1 1148 1214 2002 3216 3516 3983 4006 4341
Size: 1147 66 788 1214 300 467 23 335
Cuts at: 4341 4587 5599 5685 5697 6043 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1214 1147 1012 788 467 449 346 335
300 246 86 66 23 12
BstZ17I GTA'TAC
Cuts at: 698 698
Size: 6491
Bsu36I CC'TnA_GG
Cuts at: 2463 2463
Size: 6491
BtgI C'CryG_G
Cuts at: 107 238 943 1131 1532 1784 2579 107
Size: 131 705 188 401 252 795 4019
Fragments arranged by size:
4019 795 705 401 252 188 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2015 4441 4622 4785 2015
Size: 2426 181 163 3721
Fragments arranged by size:
3721 2426 181 163
BtsI GCAGTG_nn'
Cuts at: 170 526 674 2245 3734 170
Size: 356 148 1571 1489 2927
Fragments arranged by size:
2927 1571 1489 356 148
DraI TTT'AAA
Cuts at: 3490 3490
Size: 6491
DraIII CAC_nnn'GTG
Cuts at: 1137 1260 1137
Size: 123 6368
DrdI GACnn_nn'nnGTC
Cuts at: 4197 5055 4197
Size: 858 5633
EaeI y'GGCC_r
Cuts at: 285 388 621 674 853 962 1499 2109 2357
Size: 103 233 53 179 109 537 610 248
Cuts at: 2357 2991 4076 4250 4641 4668 6013 285
Size: 634 1085 174 391 27 1345 763
Fragments arranged by size:
1345 1085 763 634 610 537 391 248
233 179 174 109 103 53 27
EagI C'GGCC_G
Cuts at: 621 674 2109 4076 621
Size: 53 1435 1967 3036
Fragments arranged by size:
3036 1967 1435 53
EarI CTCTTCn'nnn_
Cuts at: 202 847 3177 3189 4514 4724 202
Size: 645 2330 12 1325 210 1969
Fragments arranged by size:
2330 1969 1325 645 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 889 1644 4699 5009 5155 6301 889
Size: 755 3055 310 146 1146 1079
Fragments arranged by size:
3055 1146 1079 755 310 146
EcoICRI GAG'CTC
Cuts at: 2837 3366 3503 2837
Size: 529 137 5825
Fragments arranged by size:
5825 529 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 356 565 889 1447 1561 1762 1955 2455
Size: 63 209 324 558 114 201 193 500
Cuts at: 2455 2850 2955 4316 4748 5495 5763 293
Size: 395 105 1361 432 747 268 1021
Fragments arranged by size:
1361 1021 747 558 500 432 395 324
268 209 201 193 114 105 63
EcoNI CCTnn'n_nnAGG
Cuts at: 640 2592 3351 640
Size: 1952 759 3780
Fragments arranged by size:
3780 1952 759
EcoO109I rG'GnC_Cy
Cuts at: 181 229 322 1421 1806 1905 1906 2160 2217
Size: 48 93 1099 385 99 1 254 57
Cuts at: 2217 2459 2511 2518 2585 2608 2763 2791 2845
Size: 242 52 7 67 23 155 28 54
Cuts at: 2845 2906 3014 3148 3157 3268 3295 3303 3304
Size: 61 108 134 9 111 27 8 1
Cuts at: 3304 3377 3630 181
Size: 73 253 3042
Fragments arranged by size:
3042 1099 385 254 253 242 155 134
111 108 99 93 73 67 61 57
54 52 48 28 27 23 9 8
7 1 1
EcoRI G'AATT_C
Cuts at: 3495 3495
Size: 6491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 819 1299 1905 2793 4510 819
Size: 480 606 888 1717 2800
Fragments arranged by size:
2800 1717 888 606 480
FseI GG_CCGG'CC
Cuts at: 968 968
Size: 6491
FspI TGC'GCA
Cuts at: 4272 4272
Size: 6491
HaeII r_GCGC'y
Cuts at: 712 3108 3925 4173 5195 6174 712
Size: 2396 817 248 1022 979 1029
Fragments arranged by size:
2396 1029 1022 979 817 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 620 634 652 666 706 728 730
Size: 32 50 14 18 14 40 22 2
Cuts at: 730 738 760 762 1330 1362 1528 1560 2011
Size: 8 22 2 568 32 166 32 451
Cuts at: 2011 2043 2224 2256 4333 4363 4365 4395 538
Size: 32 181 32 2077 30 2 30 2634
Fragments arranged by size:
2634 2077 568 451 181 166 50 40
32 32 32 32 32 30 30 22
22 18 14 14 8 2 2 2
HincII GTy'rAC
Cuts at: 279 3135 3530 279
Size: 2856 395 3240
Fragments arranged by size:
3240 2856 395
KasI G'GCGC_C
Cuts at: 708 3104 3921 4169 708
Size: 2396 817 248 3030
Fragments arranged by size:
3030 2396 817 248
KpnI G_GTAC'C
Cuts at: 3511 3511
Size: 6491
MluI A'CGCG_T
Cuts at: 5924 5924
Size: 6491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1199 5162 5346 1199
Size: 3963 184 2344
Fragments arranged by size:
3963 2344 184
MscI TGG'CCA
Cuts at: 287 1501 2993 4252 287
Size: 1214 1492 1259 2526
Fragments arranged by size:
2526 1492 1259 1214
MslI CAynn'nnrTG
Cuts at: 601 982 1348 1480 1621 2501 4480 4607 601
Size: 381 366 132 141 880 1979 127 2485
Fragments arranged by size:
2485 1979 880 381 366 141 132 127
MspA1I CmG'CkG
Cuts at: 240 937 1008 1020 1401 1679 1715 1857 1877
Size: 697 71 12 381 278 36 142 20
Cuts at: 1877 2042 2166 2574 3446 3602 3916 4276 5289
Size: 165 124 408 872 156 314 360 1013
Cuts at: 5289 5534 240
Size: 245 1197
Fragments arranged by size:
1197 1013 872 697 408 381 360 314
278 245 165 156 142 124 71 36
20 12
NaeI GCC'GGC
Cuts at: 625 966 1659 1771 4672 625
Size: 341 693 112 2901 2444
Fragments arranged by size:
2901 2444 693 341 112
NarI GG'CG_CC
Cuts at: 709 3105 3922 4170 709
Size: 2396 817 248 3030
Fragments arranged by size:
3030 2396 817 248
NcoI C'CATG_G
Cuts at: 107 1532 2579 107
Size: 1425 1047 4019
Fragments arranged by size:
4019 1425 1047
NgoMIV G'CCGG_C
Cuts at: 623 964 1657 1769 4670 623
Size: 341 693 112 2901 2444
Fragments arranged by size:
2901 2444 693 341 112
NotI GC'GGCC_GC
Cuts at: 674 674
Size: 6491
NsiI A_TGCA'T
Cuts at: 1487 1487
Size: 6491
NspI r_CATG'y
Cuts at: 762 1485 1489 2988 3544 4044 4951 762
Size: 723 4 1499 556 500 907 2302
Fragments arranged by size:
2302 1499 907 723 556 500 4
PasI CC'CwG_GG
Cuts at: 142 912 1764 2214 2278 2454 2735 2787 3152
Size: 770 852 450 64 176 281 52 365
Cuts at: 3152 3263 3299 142
Size: 111 36 3334
Fragments arranged by size:
3334 852 770 450 365 281 176 111
64 52 36
PciI A'CATG_T
Cuts at: 4947 4947
Size: 6491
PflMI CCAn_nnn'nTGG
Cuts at: 519 1137 1508 519
Size: 618 371 5502
Fragments arranged by size:
5502 618 371
PfoI T'CCnGG_A
Cuts at: 1217 1574 2932 1217
Size: 357 1358 4776
Fragments arranged by size:
4776 1358 357
PmeI GTTT'AAAC
Cuts at: 3490 3490
Size: 6491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 337 369 5630 5662 337
Size: 32 5261 32 1166
Fragments arranged by size:
5261 1166 32 32
PpuMI rG'GwC_Cy
Cuts at: 1421 2459 2511 2518 2585 2763 2906 3014 3157
Size: 1038 52 7 67 178 143 108 143
Cuts at: 3157 3268 3295 3377 1421
Size: 111 27 82 4535
Fragments arranged by size:
4535 1038 178 143 143 111 108 82
67 52 27 7
PspOMI G'GGCC_C
Cuts at: 181 322 1905 2217 2790 2844 3148 3303 181
Size: 141 1583 312 573 54 304 155 3369
Fragments arranged by size:
3369 1583 573 312 304 155 141 54
PspXI vC'TCGA_Gb
Cuts at: 224 224
Size: 6491
PstI C_TGCA'G
Cuts at: 539 669 1247 1674 3538 4223 539
Size: 130 578 427 1864 685 2807
Fragments arranged by size:
2807 1864 685 578 427 130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6491
PvuII CAG'CTG
Cuts at: 937 1401 1715 1877 3446 3916 4276 937
Size: 464 314 162 1569 470 360 3152
Fragments arranged by size:
3152 1569 470 464 360 314 162
RsrII CG'GwC_CG
Cuts at: 4686 4686
Size: 6491
SacI G_AGCT'C
Cuts at: 2839 3368 3505 2839
Size: 529 137 5825
Fragments arranged by size:
5825 529 137
SacII CC_GC'GG
Cuts at: 241 241
Size: 6491
SalI G'TCGA_C
Cuts at: 3528 3528
Size: 6491
SanDI GG'GwC_CC
Cuts at: 2511 2518 2763 2906 3157 3268 3295 2511
Size: 7 245 143 251 111 27 5707
Fragments arranged by size:
5707 251 245 143 111 27 7
SapI GCTCTTCn'nnn_
Cuts at: 4514 4724 4514
Size: 210 6281
SbfI CC_TGCA'GG
Cuts at: 1247 1674 3538 1247
Size: 427 1864 4200
Fragments arranged by size:
4200 1864 427
ScaI AGT'ACT
Cuts at: 6008 6008
Size: 6491
SfcI C'TryA_G
Cuts at: 33 535 665 1243 1670 3327 3534 4219 5212
Size: 502 130 578 427 1657 207 685 993
Cuts at: 5212 5403 33
Size: 191 1121
Fragments arranged by size:
1657 1121 993 685 578 502 427 207
191 130
SfoI GGC'GCC
Cuts at: 710 3106 3923 4171 710
Size: 2396 817 248 3030
Fragments arranged by size:
3030 2396 817 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6491
SgrAI Cr'CCGG_yG
Cuts at: 440 440
Size: 6491
SmaI CCC'GGG
Cuts at: 2501 2516 3513 2501
Size: 15 997 5479
Fragments arranged by size:
5479 997 15
SmlI C'TyrA_G
Cuts at: 224 2589 3646 4561 5053 5315 224
Size: 2365 1057 915 492 262 1400
Fragments arranged by size:
2365 1400 1057 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6491
SphI G_CATG'C
Cuts at: 1485 1489 2988 3544 4044 1485
Size: 4 1499 556 500 3932
Fragments arranged by size:
3932 1499 556 500 4
StyI C'CwwG_G
Cuts at: 107 1532 1802 2536 2579 3072 3625 3979 107
Size: 1425 270 734 43 493 553 354 2619
Fragments arranged by size:
2619 1425 734 553 493 354 270 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 780 1530 1546 1633 1777 2215 4074 4207 4666
Size: 750 16 87 144 438 1859 133 459
Cuts at: 4666 4875 780
Size: 209 2396
Fragments arranged by size:
2396 1859 750 459 438 209 144 133
87 16
TatI w'GTAC_w
Cuts at: 2879 3383 6006 2879
Size: 504 2623 3364
Fragments arranged by size:
3364 2623 504
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 644 676 1246 1278 1320 1352 2990 3022 3361
Size: 32 570 32 42 32 1638 32 339
Cuts at: 3361 3393 6008 6040 644
Size: 32 2615 32 1095
Fragments arranged by size:
2615 1638 1095 570 339 42 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 552 742 1990 4288 552
Size: 190 1248 2298 2755
Fragments arranged by size:
2755 2298 1248 190
XbaI T'CTAG_A
Cuts at: 3522 3522
Size: 6491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 831 2380 2825 3986 831
Size: 1549 445 1161 3336
Fragments arranged by size:
3336 1549 1161 445
XhoI C'TCGA_G
Cuts at: 224 224
Size: 6491
XmaI C'CCGG_G
Cuts at: 2499 2514 3511 2499
Size: 15 997 5479
Fragments arranged by size:
5479 997 15
XmnI GAAnn'nnTTC
Cuts at: 1885 3210 1885
Size: 1325 5166
ZraI GAC'GTC
Cuts at: 1629 1629
Size: 6491
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BmgBI BmtI
BsiWI BssHII ClaI CspCI EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NruI PacI PmlI PshAI PsiI
PsrI SexAI SfiI SnaBI SrfI SspI StuI SwaI