Restriction map for
pF1KE2496
(Circular) (Six-base) MAPSORT of: pF1KE2496.seq Check: 989 from: 1 to: 5078
pF1KE2496 5078 bp
With 182 enzymes: SgfI *
January 16, 2014 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1425 1499 2033 1425
Size: 74 534 4470
Fragments arranged by size:
4470 534 74
AatII G_ACGT'C
Cuts at: 1274 1625 1274
Size: 351 4727
AccI GT'mk_AC
Cuts at: 809 1280 2116 809
Size: 471 836 3771
Fragments arranged by size:
3771 836 471
Acc65I G'GTAC_C
Cuts at: 2094 2094
Size: 5078
AclI AA'CG_TT
Cuts at: 4918 4918
Size: 5078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 896 1528 1924 2903 3335 4082 4350 642
Size: 254 632 396 979 432 747 268 1370
Fragments arranged by size:
1370 979 747 632 432 396 268 254
AflIII A'CryG_T
Cuts at: 2430 3534 4511 2430
Size: 1104 977 2997
Fragments arranged by size:
2997 1104 977
AgeI A'CCGG_T
Cuts at: 3466 3466
Size: 5078
AleI CACnn'nnGTG
Cuts at: 767 767
Size: 5078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1507 1507
Size: 5078
AlwNI CAG_nnn'CTG
Cuts at: 733 796 2383 3950 733
Size: 63 1587 1567 1861
Fragments arranged by size:
1861 1587 1567 63
ApaI G_GGCC'C
Cuts at: 174 931 174
Size: 757 4321
ApaLI G'TGCA_C
Cuts at: 1794 3848 1794
Size: 2054 3024
ApoI r'AATT_y
Cuts at: 611 1984 2082 4431 4687 611
Size: 1373 98 2349 256 1002
Fragments arranged by size:
2349 1373 1002 256 98
AseI AT'TA_AT
Cuts at: 20 20
Size: 5078
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
AvaI C'yCGr_G
Cuts at: 698 709 1001 1600 1875 2098 698
Size: 11 292 599 275 223 3678
Fragments arranged by size:
3678 599 292 275 223 11
BamHI G'GATC_C
Cuts at: 2103 2103
Size: 5078
BanI G'GyrC_C
Cuts at: 761 1060 1124 2094 2508 2756 2791 761
Size: 299 64 970 414 248 35 3048
Fragments arranged by size:
3048 970 414 299 248 64 35
BanII G_rGCy'C
Cuts at: 174 931 1419 1876 2092 3122 174
Size: 757 488 457 216 1030 2130
Fragments arranged by size:
2130 1030 757 488 457 216
BbeI G_GCGC'C
Cuts at: 2512 2760 2512
Size: 248 4830
BbsI GAAGACnn'nnnn_
Cuts at: 416 2045 416
Size: 1629 3449
BciVI GTATCCnnnnn_n'
Cuts at: 2976 3737 5078 2976
Size: 761 1341 2976
Fragments arranged by size:
2976 1341 761
BclI T'GATC_A
Cuts at: 2598 2598
Size: 5078
BglI GCCn_nnn'nGGC
Cuts at: 430 935 430
Size: 505 4573
BglII A'GATC_T
Cuts at: 2593 4630 2593
Size: 2037 3041
BlpI GC'TnA_GC
Cuts at: 2190 2190
Size: 5078
Bme1580I G_kGCm'C
Cuts at: 174 460 931 1798 1960 2703 2796 3852 174
Size: 286 471 867 162 743 93 1056 1400
Fragments arranged by size:
1400 1056 867 743 471 286 162 93
BmgBI CAC'GTC
Cuts at: 1359 1359
Size: 5078
BmrI ACTGGGnnnn_n'
Cuts at: 2290 2371 2706 4850 2290
Size: 81 335 2144 2518
Fragments arranged by size:
2518 2144 335 81
BmtI G_CTAG'C
Cuts at: 656 656
Size: 5078
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 5078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 1843 1693
Size: 150 4928
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2254 3133 3625 3923 1411
Size: 843 879 492 298 2566
Fragments arranged by size:
2566 879 843 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1158 4782 1158
Size: 3624 1454
BsaAI yAC'GTr
Cuts at: 2431 2431
Size: 5078
BsaBI GATnn'nnATC
Cuts at: 6 2592 4629 6
Size: 2586 2037 455
Fragments arranged by size:
2586 2037 455
BsaHI Gr'CG_yC
Cuts at: 943 1271 1622 2509 2757 3459 4955 943
Size: 328 351 887 248 702 1496 1066
Fragments arranged by size:
1496 1066 887 702 351 328 248
BsaWI w'CCGG_w
Cuts at: 1520 2267 2788 3466 3740 3887 4298 1520
Size: 747 521 678 274 147 411 2300
Fragments arranged by size:
2300 747 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 607 637 1726 1756 1939 1969 607
Size: 30 1089 30 183 30 3716
Fragments arranged by size:
3716 1089 183 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 1318 1569 1618 1647 1747 1846 1960 2077
Size: 447 251 49 29 100 99 114 117
Cuts at: 2077 2080 871
Size: 3 3869
Fragments arranged by size:
3869 447 251 117 114 100 99 49
29 3
BseYI C'CCAG_C
Cuts at: 789 1027 2000 2503 3261 3838 4334 789
Size: 238 973 503 758 577 496 1533
Fragments arranged by size:
1533 973 758 577 503 496 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 267 1094 1225 1453 1467 139
Size: 128 827 131 228 14 3750
Fragments arranged by size:
3750 827 228 131 128 14
BsiEI CG_ry'CG
Cuts at: 104 867 1917 2620 2666 3874 104
Size: 763 1050 703 46 1208 1308
Fragments arranged by size:
1308 1208 1050 763 703 46
BsiHKAI G_wGCw'C
Cuts at: 1419 1798 1876 2092 2870 3060 3852 1419
Size: 379 78 216 778 190 792 2645
Fragments arranged by size:
2645 792 778 379 216 190 78
Bsp1286I G_dGCh'C
Cuts at: 174 460 931 1419 1798 1876 1960 2092 2703
Size: 286 471 488 379 78 84 132 611
Cuts at: 2703 2796 2870 3060 3122 3852 174
Size: 93 74 190 62 730 1400
Fragments arranged by size:
1400 730 611 488 471 379 286 190
132 93 84 78 74 62
BspEI T'CCGG_A
Cuts at: 4298 4298
Size: 5078
BspHI T'CATG_A
Cuts at: 4254 4254
Size: 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1425 1499 1547 1817 2033 2128 2342 2644 3025
Size: 74 48 270 216 95 214 302 381
Cuts at: 3025 1425
Size: 3478
Fragments arranged by size:
3478 381 302 270 216 214 95 74
48
BsrBI CCG'CTC
Cuts at: 50 3370 3424 4908 5073 50
Size: 3320 54 1484 165 55
Fragments arranged by size:
3320 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 591 2990 591
Size: 2399 2679
BsrFI r'CCGG_y
Cuts at: 203 383 424 866 1916 3076 3257 3466 203
Size: 180 41 442 1050 1160 181 209 1815
Fragments arranged by size:
1815 1160 1050 442 209 181 180 41
BsrGI T'GTAC_A
Cuts at: 116 1079 116
Size: 963 4115
BssSI C'ACGA_G
Cuts at: 3349 3707 3349
Size: 358 4720
BstAPI GCAn_nnn'nTGC
Cuts at: 2455 2455
Size: 5078
BstBI TT'CG_AA
Cuts at: 3439 3439
Size: 5078
BstXI CCAn_nnnn'nTGG
Cuts at: 191 309 191
Size: 118 4960
BstYI r'GATC_y
Cuts at: 1 2103 2570 2593 2928 3174 4186 4272 4284
Size: 2102 467 23 335 246 1012 86 12
Cuts at: 4284 4630 1
Size: 346 449
Fragments arranged by size:
2102 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1451 1451
Size: 5078
BtgI C'CryG_G
Cuts at: 107 1741 107
Size: 1634 3444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 701 1461 3028 3209 3372 701
Size: 760 1567 181 163 2407
Fragments arranged by size:
2407 1567 760 181 163
BtsI GCAGTG_nn'
Cuts at: 284 367 488 2321 284
Size: 83 121 1833 3041
Fragments arranged by size:
3041 1833 121 83
DraI TTT'AAA
Cuts at: 2077 2077
Size: 5078
DraIII CAC_nnn'GTG
Cuts at: 162 162
Size: 5078
DrdI GACnn_nn'nnGTC
Cuts at: 1150 1276 2784 3642 1150
Size: 126 1508 858 2586
Fragments arranged by size:
2586 1508 858 126
EaeI y'GGCC_r
Cuts at: 422 864 868 1914 2663 2837 3228 3255 4600
Size: 442 4 1046 749 174 391 27 1345
Cuts at: 4600 422
Size: 900
Fragments arranged by size:
1345 1046 900 749 442 391 174 27
4
EagI C'GGCC_G
Cuts at: 864 1914 2663 864
Size: 1050 749 3279
Fragments arranged by size:
3279 1050 749
EarI CTCTTCn'nnn_
Cuts at: 298 361 3101 3311 298
Size: 63 2740 210 2065
Fragments arranged by size:
2740 2065 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 138 1146 1905 3286 3596 3742 4888 138
Size: 1008 759 1381 310 146 1146 328
Fragments arranged by size:
1381 1146 1008 759 328 310 146
EcoICRI GAG'CTC
Cuts at: 1417 1874 2090 1417
Size: 457 216 4405
Fragments arranged by size:
4405 457 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 896 1528 1693 1843 1924 2903 3335 4082
Size: 254 632 165 150 81 979 432 747
Cuts at: 4082 4350 642
Size: 268 1370
Fragments arranged by size:
1370 979 747 632 432 268 254 165
150 81
EcoNI CCTnn'n_nnAGG
Cuts at: 1015 1081 1195 1543 1813 1015
Size: 66 114 348 270 4280
Fragments arranged by size:
4280 348 270 114 66
EcoO109I rG'GnC_Cy
Cuts at: 171 417 463 801 927 1707 1723 1818 2217
Size: 246 46 338 126 780 16 95 399
Cuts at: 2217 171
Size: 3032
Fragments arranged by size:
3032 780 399 338 246 126 95 46
16
EcoRI G'AATT_C
Cuts at: 1984 2082 1984
Size: 98 4980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 5078
FseI GG_CCGG'CC
Cuts at: 870 870
Size: 5078
FspI TGC'GCA
Cuts at: 844 2859 844
Size: 2015 3063
FspAI rTGC'GCAy
Cuts at: 844 844
Size: 5078
HaeII r_GCGC'y
Cuts at: 246 650 1517 2512 2760 3782 4761 246
Size: 404 867 995 248 1022 979 563
Fragments arranged by size:
1022 995 979 867 563 404 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1639 1671 2920 2950 2952 2982 1639
Size: 32 1249 30 2 30 3735
Fragments arranged by size:
3735 1249 32 30 30 2
HincII GTy'rAC
Cuts at: 531 810 2117 531
Size: 279 1307 3492
Fragments arranged by size:
3492 1307 279
HindIII A'AGCT_T
Cuts at: 474 474
Size: 5078
KasI G'GCGC_C
Cuts at: 2508 2756 2508
Size: 248 4830
KpnI G_GTAC'C
Cuts at: 2098 2098
Size: 5078
MluI A'CGCG_T
Cuts at: 4511 4511
Size: 5078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3749 3933 3749
Size: 184 4894
MscI TGG'CCA
Cuts at: 2839 2839
Size: 5078
MslI CAynn'nnrTG
Cuts at: 767 1357 1493 3067 3194 767
Size: 590 136 1574 127 2651
Fragments arranged by size:
2651 1574 590 136 127
MspA1I CmG'CkG
Cuts at: 140 249 276 1049 1239 1322 1533 2004 2189
Size: 109 27 773 190 83 211 471 185
Cuts at: 2189 2503 2863 3876 4121 140
Size: 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 773 471 360 314 245 211
190 185 109 83 27
NaeI GCC'GGC
Cuts at: 385 868 1918 3259 385
Size: 483 1050 1341 2204
Fragments arranged by size:
2204 1341 1050 483
NarI GG'CG_CC
Cuts at: 2509 2757 2509
Size: 248 4830
NcoI C'CATG_G
Cuts at: 107 107
Size: 5078
NgoMIV G'CCGG_C
Cuts at: 383 866 1916 3257 383
Size: 483 1050 1341 2204
Fragments arranged by size:
2204 1341 1050 483
NheI G'CTAG_C
Cuts at: 652 652
Size: 5078
NspI r_CATG'y
Cuts at: 1498 1935 1942 2131 2631 3538 1498
Size: 437 7 189 500 907 3038
Fragments arranged by size:
3038 907 500 437 189 7
PciI A'CATG_T
Cuts at: 3534 3534
Size: 5078
PflMI CCAn_nnn'nTGG
Cuts at: 796 796
Size: 5078
PfoI T'CCnGG_A
Cuts at: 1820 1820
Size: 5078
PmeI GTTT'AAAC
Cuts at: 2077 2077
Size: 5078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 5046
PpuMI rG'GwC_Cy
Cuts at: 801 1707 1818 801
Size: 906 111 4061
Fragments arranged by size:
4061 906 111
PspOMI G'GGCC_C
Cuts at: 170 927 170
Size: 757 4321
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1762 1794 1762
Size: 32 5046
PstI C_TGCA'G
Cuts at: 275 374 1196 1303 1814 2125 2810 275
Size: 99 822 107 511 311 685 2543
Fragments arranged by size:
2543 822 685 511 311 107 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5078
PvuII CAG'CTG
Cuts at: 140 276 1239 1533 2004 2503 2863 140
Size: 136 963 294 471 499 360 2355
Fragments arranged by size:
2355 963 499 471 360 294 136
RsrII CG'GwC_CG
Cuts at: 3273 3273
Size: 5078
SacI G_AGCT'C
Cuts at: 1419 1876 2092 1419
Size: 457 216 4405
Fragments arranged by size:
4405 457 216
SalI G'TCGA_C
Cuts at: 808 2115 808
Size: 1307 3771
SanDI GG'GwC_CC
Cuts at: 801 1707 801
Size: 906 4172
SapI GCTCTTCn'nnn_
Cuts at: 3101 3311 3101
Size: 210 4868
SbfI CC_TGCA'GG
Cuts at: 1196 2125 1196
Size: 929 4149
ScaI AGT'ACT
Cuts at: 4595 4595
Size: 5078
SfcI C'TryA_G
Cuts at: 33 271 370 1192 1299 1701 1810 2121 2806
Size: 238 99 822 107 402 109 311 685
Cuts at: 2806 3799 3990 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 822 685 402 311 238 191
109 107 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 935 935
Size: 5078
SfoI GGC'GCC
Cuts at: 2510 2758 2510
Size: 248 4830
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
SmaI CCC'GGG
Cuts at: 700 711 1003 2100 700
Size: 11 292 1097 3678
Fragments arranged by size:
3678 1097 292 11
SmlI C'TyrA_G
Cuts at: 1426 2233 3148 3640 3902 1426
Size: 807 915 492 262 2602
Fragments arranged by size:
2602 915 807 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5078
SphI G_CATG'C
Cuts at: 1498 1942 2131 2631 1498
Size: 444 189 500 3945
Fragments arranged by size:
3945 500 444 189
SrfI GCCC'GGGC
Cuts at: 700 700
Size: 5078
StuI AGG'CCT
Cuts at: 1087 1138 1087
Size: 51 5027
StyI C'CwwG_G
Cuts at: 107 560 931 2212 2566 107
Size: 453 371 1281 354 2619
Fragments arranged by size:
2619 1281 453 371 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 1711 2014 2661 2794 3253 3462 562
Size: 1149 303 647 133 459 209 2178
Fragments arranged by size:
2178 1149 647 459 303 209 133
TatI w'GTAC_w
Cuts at: 116 740 1079 1256 4593 116
Size: 624 339 177 3337 601
Fragments arranged by size:
3337 624 601 339 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 1171 1203 1726 1758 1939 1971 4595
Size: 32 991 32 523 32 181 32 2624
Cuts at: 4595 4627 148
Size: 32 599
Fragments arranged by size:
2624 991 599 523 181 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 805 1624 2875 805
Size: 819 1251 3008
Fragments arranged by size:
3008 1251 819
XbaI T'CTAG_A
Cuts at: 2109 2109
Size: 5078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1356 2043 2573 1356
Size: 687 530 3861
Fragments arranged by size:
3861 687 530
XmaI C'CCGG_G
Cuts at: 698 709 1001 2098 698
Size: 11 292 1097 3678
Fragments arranged by size:
3678 1097 292 11
ZraI GAC'GTC
Cuts at: 1272 1623 1272
Size: 351 4727
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI Bpu10I BsiWI BsmI BsmBI BssHII BstEII
BstZ17I ClaI CspCI EcoRV HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
SacII SexAI SgrAI SnaBI SspI SwaI XhoI XmnI