Restriction map for
pF1KE2493
(Circular) (Six-base) MAPSORT of: pF1KE2493.seq Check: 7490 from: 1 to: 3902
pF1KE2493 3902 bp
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AccI GT'mk_AC
Cuts at: 940 940
Size: 3902
Acc65I G'GTAC_C
Cuts at: 918 918
Size: 3902
AclI AA'CG_TT
Cuts at: 3742 3742
Size: 3902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 408 1727 2159 2906 3174 272
Size: 136 1319 432 747 268 1000
Fragments arranged by size:
1319 1000 747 432 268 136
AflIII A'CryG_T
Cuts at: 1254 2358 3335 1254
Size: 1104 977 1821
Fragments arranged by size:
1821 1104 977
AgeI A'CCGG_T
Cuts at: 2290 2290
Size: 3902
AlwNI CAG_nnn'CTG
Cuts at: 892 1207 2774 892
Size: 315 1567 2020
Fragments arranged by size:
2020 1567 315
ApaI G_GGCC'C
Cuts at: 581 581
Size: 3902
ApaLI G'TGCA_C
Cuts at: 702 2672 702
Size: 1970 1932
ApoI r'AATT_y
Cuts at: 906 3255 3511 906
Size: 2349 256 1297
Fragments arranged by size:
2349 1297 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3902
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
AvaI C'yCGr_G
Cuts at: 496 922 496
Size: 426 3476
BamHI G'GATC_C
Cuts at: 927 927
Size: 3902
BanI G'GyrC_C
Cuts at: 520 918 1332 1580 1615 520
Size: 398 414 248 35 2807
Fragments arranged by size:
2807 414 398 248 35
BanII G_rGCy'C
Cuts at: 245 505 581 622 916 1946 245
Size: 260 76 41 294 1030 2201
Fragments arranged by size:
2201 1030 294 260 76 41
BbeI G_GCGC'C
Cuts at: 1336 1584 1336
Size: 248 3654
BbsI GAAGACnn'nnnn_
Cuts at: 834 834
Size: 3902
BbvCI CC'TCA_GC
Cuts at: 507 507
Size: 3902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3868
BciVI GTATCCnnnnn_n'
Cuts at: 346 1800 2561 3902 346
Size: 1454 761 1341 346
Fragments arranged by size:
1454 1341 761 346
BclI T'GATC_A
Cuts at: 1422 1422
Size: 3902
BglI GCCn_nnn'nGGC
Cuts at: 323 323
Size: 3902
BglII A'GATC_T
Cuts at: 1417 3454 1417
Size: 2037 1865
BlpI GC'TnA_GC
Cuts at: 1014 1014
Size: 3902
Bme1580I G_kGCm'C
Cuts at: 581 706 1527 1620 2676 581
Size: 125 821 93 1056 1807
Fragments arranged by size:
1807 1056 821 125 93
BmrI ACTGGGnnnn_n'
Cuts at: 1114 1195 1530 3674 1114
Size: 81 335 2144 1342
Fragments arranged by size:
2144 1342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 264 522 2269 264
Size: 258 1747 1897
Fragments arranged by size:
1897 1747 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 3902
Bpu10I CC'TnA_GC
Cuts at: 507 507
Size: 3902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1078 1957 2449 2747 253
Size: 825 879 492 298 1408
Fragments arranged by size:
1408 879 825 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 572 685 3606 572
Size: 113 2921 868
Fragments arranged by size:
2921 868 113
BsaAI yAC'GTr
Cuts at: 1255 1255
Size: 3902
BsaBI GATnn'nnATC
Cuts at: 6 1416 3453 6
Size: 1410 2037 455
Fragments arranged by size:
2037 1410 455
BsaHI Gr'CG_yC
Cuts at: 326 362 1333 1581 2283 3779 326
Size: 36 971 248 702 1496 449
Fragments arranged by size:
1496 971 702 449 248 36
BsaWI w'CCGG_w
Cuts at: 611 1091 1612 2290 2564 2711 3122 611
Size: 480 521 678 274 147 411 1391
Fragments arranged by size:
1391 678 521 480 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 475 445
Size: 30 3872
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 495 640 726 756 495
Size: 145 86 30 3641
Fragments arranged by size:
3641 145 86 30
BseYI C'CCAG_C
Cuts at: 1327 2085 2662 3158 1327
Size: 758 577 496 2071
Fragments arranged by size:
2071 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 451 552 423
Size: 28 101 3773
Fragments arranged by size:
3773 101 28
BsiEI CG_ry'CG
Cuts at: 104 1444 1490 2698 104
Size: 1340 46 1208 1308
Fragments arranged by size:
1340 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 245 277 410 706 916 1694 1884 2676 245
Size: 32 133 296 210 778 190 792 1471
Fragments arranged by size:
1471 792 778 296 210 190 133 32
BsmI GAATG_Cn'
Cuts at: 777 777
Size: 3902
Bsp1286I G_dGCh'C
Cuts at: 245 277 410 505 581 622 706 916 1527
Size: 32 133 95 76 41 84 210 611
Cuts at: 1527 1620 1694 1884 1946 2676 245
Size: 93 74 190 62 730 1471
Fragments arranged by size:
1471 730 611 210 190 133 95 93
84 76 74 62 41 32
BspEI T'CCGG_A
Cuts at: 611 3122 611
Size: 2511 1391
BspHI T'CATG_A
Cuts at: 3078 3078
Size: 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 473 952 1166 1468 1849 473
Size: 479 214 302 381 2526
Fragments arranged by size:
2526 479 381 302 214
BsrBI CCG'CTC
Cuts at: 50 630 2194 2248 3732 3897 50
Size: 580 1564 54 1484 165 55
Fragments arranged by size:
1564 1484 580 165 55 54
BsrDI GCAATG_nn'
Cuts at: 553 1814 553
Size: 1261 2641
BsrFI r'CCGG_y
Cuts at: 761 1900 2081 2290 761
Size: 1139 181 209 2373
Fragments arranged by size:
2373 1139 209 181
BssSI C'ACGA_G
Cuts at: 2173 2531 2173
Size: 358 3544
BstAPI GCAn_nnn'nTGC
Cuts at: 395 892 1279 395
Size: 497 387 3018
Fragments arranged by size:
3018 497 387
BstBI TT'CG_AA
Cuts at: 2263 2263
Size: 3902
BstYI r'GATC_y
Cuts at: 1 927 1394 1417 1752 1998 3010 3096 3108
Size: 926 467 23 335 246 1012 86 12
Cuts at: 3108 3454 1
Size: 346 449
Fragments arranged by size:
1012 926 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 728 728
Size: 3902
BtgI C'CryG_G
Cuts at: 524 571 524
Size: 47 3855
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1852 2033 2196 1852
Size: 181 163 3558
Fragments arranged by size:
3558 181 163
BtsI GCAGTG_nn'
Cuts at: 1145 1145
Size: 3902
DraI TTT'AAA
Cuts at: 901 901
Size: 3902
DraIII CAC_nnn'GTG
Cuts at: 446 446
Size: 3902
DrdI GACnn_nn'nnGTC
Cuts at: 1608 2466 1608
Size: 858 3044
EaeI y'GGCC_r
Cuts at: 788 1487 1661 2052 2079 3424 788
Size: 699 174 391 27 1345 1266
Fragments arranged by size:
1345 1266 699 391 174 27
EagI C'GGCC_G
Cuts at: 1487 1487
Size: 3902
EarI CTCTTCn'nnn_
Cuts at: 733 1925 2135 733
Size: 1192 210 2500
Fragments arranged by size:
2500 1192 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 213 304 2110 2420 2566 3712 213
Size: 91 1806 310 146 1146 403
Fragments arranged by size:
1806 1146 403 310 146 91
EcoICRI GAG'CTC
Cuts at: 243 914 243
Size: 671 3231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 319 408 1727 2159 2906 3174 272
Size: 47 89 1319 432 747 268 1000
Fragments arranged by size:
1319 1000 747 432 268 89 47
EcoNI CCTnn'n_nnAGG
Cuts at: 469 469
Size: 3902
EcoO109I rG'GnC_Cy
Cuts at: 577 578 889 1041 577
Size: 1 311 152 3438
Fragments arranged by size:
3438 311 152 1
EcoRI G'AATT_C
Cuts at: 906 906
Size: 3902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 474 1921 474
Size: 1447 2455
FspI TGC'GCA
Cuts at: 545 1683 545
Size: 1138 2764
HaeII r_GCGC'y
Cuts at: 1336 1584 2606 3585 1336
Size: 248 1022 979 1653
Fragments arranged by size:
1653 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1744 1774 1776 1806 1744
Size: 30 2 30 3840
Fragments arranged by size:
3840 30 30 2
HincII GTy'rAC
Cuts at: 258 941 258
Size: 683 3219
KasI G'GCGC_C
Cuts at: 1332 1580 1332
Size: 248 3654
KpnI G_GTAC'C
Cuts at: 922 922
Size: 3902
MluI A'CGCG_T
Cuts at: 3335 3335
Size: 3902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2573 2757 2573
Size: 184 3718
MscI TGG'CCA
Cuts at: 790 1663 790
Size: 873 3029
MslI CAynn'nnrTG
Cuts at: 1891 2018 1891
Size: 127 3775
MspA1I CmG'CkG
Cuts at: 479 511 1013 1327 1687 2700 2945 479
Size: 32 502 314 360 1013 245 1436
Fragments arranged by size:
1436 1013 502 360 314 245 32
NaeI GCC'GGC
Cuts at: 763 2083 763
Size: 1320 2582
NarI GG'CG_CC
Cuts at: 1333 1581 1333
Size: 248 3654
NcoI C'CATG_G
Cuts at: 524 524
Size: 3902
NgoMIV G'CCGG_C
Cuts at: 761 2081 761
Size: 1320 2582
NspI r_CATG'y
Cuts at: 652 955 1455 2362 652
Size: 303 500 907 2192
Fragments arranged by size:
2192 907 500 303
PciI A'CATG_T
Cuts at: 2358 2358
Size: 3902
PmeI GTTT'AAAC
Cuts at: 901 901
Size: 3902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 3870
PspOMI G'GGCC_C
Cuts at: 577 577
Size: 3902
PstI C_TGCA'G
Cuts at: 888 949 1634 888
Size: 61 685 3156
Fragments arranged by size:
3156 685 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3902
PvuII CAG'CTG
Cuts at: 511 1327 1687 511
Size: 816 360 2726
Fragments arranged by size:
2726 816 360
RsrII CG'GwC_CG
Cuts at: 2097 2097
Size: 3902
SacI G_AGCT'C
Cuts at: 245 916 245
Size: 671 3231
SalI G'TCGA_C
Cuts at: 939 939
Size: 3902
SapI GCTCTTCn'nnn_
Cuts at: 1925 2135 1925
Size: 210 3692
SbfI CC_TGCA'GG
Cuts at: 949 949
Size: 3902
ScaI AGT'ACT
Cuts at: 3419 3419
Size: 3902
SfcI C'TryA_G
Cuts at: 33 884 945 1630 2623 2814 33
Size: 851 61 685 993 191 1121
Fragments arranged by size:
1121 993 851 685 191 61
SfoI GGC'GCC
Cuts at: 1334 1582 1334
Size: 248 3654
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
SmaI CCC'GGG
Cuts at: 924 924
Size: 3902
SmlI C'TyrA_G
Cuts at: 268 1057 1972 2464 2726 268
Size: 789 915 492 262 1444
Fragments arranged by size:
1444 915 789 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3902
SphI G_CATG'C
Cuts at: 955 1455 955
Size: 500 3402
StuI AGG'CCT
Cuts at: 223 418 223
Size: 195 3707
StyI C'CwwG_G
Cuts at: 524 686 1036 1390 524
Size: 162 350 354 3036
Fragments arranged by size:
3036 354 350 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 1485 1618 2077 2286 294
Size: 1191 133 459 209 1910
Fragments arranged by size:
1910 1191 459 209 133
TatI w'GTAC_w
Cuts at: 3417 3417
Size: 3902
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 3870
Tth111I GACn'n_nGTC
Cuts at: 1699 1699
Size: 3902
XbaI T'CTAG_A
Cuts at: 933 933
Size: 3902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 783 1397 522
Size: 261 614 3027
Fragments arranged by size:
3027 614 261
XmaI C'CCGG_G
Cuts at: 922 922
Size: 3902
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI