Restriction map for
pF1KE2492
(Circular) (Six-base) MAPSORT of: pF1KE2492.seq Check: 1393 from: 1 to: 8477
pF1KE2492 8477 bp
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1404 1455 5430 1404
Size: 51 3975 4451
Fragments arranged by size:
4451 3975 51
AatII G_ACGT'C
Cuts at: 2857 5273 2857
Size: 2416 6061
AccI GT'mk_AC
Cuts at: 1468 4265 5515 1468
Size: 2797 1250 4430
Fragments arranged by size:
4430 2797 1250
Acc65I G'GTAC_C
Cuts at: 379 5493 379
Size: 5114 3363
AclI AA'CG_TT
Cuts at: 2981 8317 2981
Size: 5336 3141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 1169 2644 2938 3066 3904 5371 5470 6302
Size: 321 1475 294 128 838 1467 99 832
Cuts at: 6302 6734 7481 7749 848
Size: 432 747 268 1576
Fragments arranged by size:
1576 1475 1467 838 832 747 432 321
294 268 128 99
AflIII A'CryG_T
Cuts at: 3186 5829 6933 7910 3186
Size: 2643 1104 977 3753
Fragments arranged by size:
3753 2643 1104 977
AgeI A'CCGG_T
Cuts at: 6865 6865
Size: 8477
AleI CACnn'nnGTG
Cuts at: 829 1090 1462 4204 4724 5260 829
Size: 261 372 2742 520 536 4046
Fragments arranged by size:
4046 2742 536 520 372 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1199 1199
Size: 8477
AlwNI CAG_nnn'CTG
Cuts at: 638 2038 5782 7349 638
Size: 1400 3744 1567 1766
Fragments arranged by size:
3744 1766 1567 1400
ApaI G_GGCC'C
Cuts at: 4356 4356
Size: 8477
ApaLI G'TGCA_C
Cuts at: 936 4423 7247 936
Size: 3487 2824 2166
Fragments arranged by size:
3487 2824 2166
ApoI r'AATT_y
Cuts at: 1578 1879 2758 3575 4111 4512 5481 7830 8086
Size: 301 879 817 536 401 969 2349 256
Cuts at: 8086 1578
Size: 1969
Fragments arranged by size:
2349 1969 969 879 817 536 401 301
256
AseI AT'TA_AT
Cuts at: 20 3398 20
Size: 3378 5099
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8477
AvaI C'yCGr_G
Cuts at: 484 610 1861 3212 3907 4428 4895 5497 484
Size: 126 1251 1351 695 521 467 602 3464
Fragments arranged by size:
3464 1351 1251 695 602 521 467 126
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3601 3634 3601
Size: 33 8444
BamHI G'GATC_C
Cuts at: 480 3753 5502 480
Size: 3273 1749 3455
Fragments arranged by size:
3455 3273 1749
BanI G'GyrC_C
Cuts at: 134 379 2292 2554 4000 4497 5493 5907 6155
Size: 245 1913 262 1446 497 996 414 248
Cuts at: 6155 6190 134
Size: 35 2421
Fragments arranged by size:
2421 1913 1446 996 497 414 262 248
245 35
BanII G_rGCy'C
Cuts at: 632 892 2012 4356 4385 4902 5165 5491 6521
Size: 260 1120 2344 29 517 263 326 1030
Cuts at: 6521 632
Size: 2588
Fragments arranged by size:
2588 2344 1120 1030 517 326 263 260
29
BbeI G_GCGC'C
Cuts at: 138 2296 5911 6159 138
Size: 2158 3615 248 2456
Fragments arranged by size:
3615 2456 2158 248
BbsI GAAGACnn'nnnn_
Cuts at: 224 2431 2849 3017 3189 3938 5273 5384 224
Size: 2207 418 168 172 749 1335 111 3317
Fragments arranged by size:
3317 2207 1335 749 418 172 168 111
BbvCI CC'TCA_GC
Cuts at: 4846 4846
Size: 8477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 403 437 917 951 2821 2855 403
Size: 34 480 34 1870 34 6025
Fragments arranged by size:
6025 1870 480 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6375 7136 8477 6375
Size: 761 1341 6375
Fragments arranged by size:
6375 1341 761
BclI T'GATC_A
Cuts at: 5997 5997
Size: 8477
BglII A'GATC_T
Cuts at: 1506 2946 4938 5360 5992 8029 1506
Size: 1440 1992 422 632 2037 1954
Fragments arranged by size:
2037 1992 1954 1440 632 422
BlpI GC'TnA_GC
Cuts at: 1000 1238 5589 1000
Size: 238 4351 3888
Fragments arranged by size:
4351 3888 238
Bme1580I G_kGCm'C
Cuts at: 940 3146 4312 4356 4427 4502 6102 6195 7251
Size: 2206 1166 44 71 75 1600 93 1056
Cuts at: 7251 940
Size: 2166
Fragments arranged by size:
2206 2166 1600 1166 1056 93 75 71
44
BmgBI CAC'GTC
Cuts at: 4313 4313
Size: 8477
BmrI ACTGGGnnnn_n'
Cuts at: 1408 2454 2515 3576 3816 5689 5770 6105 8249
Size: 1046 61 1061 240 1873 81 335 2144
Cuts at: 8249 1408
Size: 1636
Fragments arranged by size:
2144 1873 1636 1061 1046 335 240 81
61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 510 804 2965 6844 510
Size: 294 2161 3879 2143
Fragments arranged by size:
3879 2161 2143 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 421 1574 2168 3488 3779 4354 5077 5179
Size: 189 1153 594 1320 291 575 723 102
Cuts at: 5179 232
Size: 3530
Fragments arranged by size:
3530 1320 1153 723 594 575 291 189
102
Bpu10I CC'TnA_GC
Cuts at: 631 1200 4846 631
Size: 569 3646 4262
Fragments arranged by size:
4262 3646 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1062 1941 5101 5377 5653 6532 7024 7322 1062
Size: 879 3160 276 276 879 492 298 2217
Fragments arranged by size:
3160 2217 879 879 492 298 276 276
BsaI GGTCTCn'nnnn_
Cuts at: 3464 8181 3464
Size: 4717 3760
BsaAI yAC'GTr
Cuts at: 3642 5830 3642
Size: 2188 6289
BsaBI GATnn'nnATC
Cuts at: 6 5991 8028 6
Size: 5985 2037 455
Fragments arranged by size:
5985 2037 455
BsaHI Gr'CG_yC
Cuts at: 135 2293 2500 2854 5270 5908 6156 6858 8354
Size: 2158 207 354 2416 638 248 702 1496
Cuts at: 8354 135
Size: 258
Fragments arranged by size:
2416 2158 1496 702 638 354 258 248
207
BsaWI w'CCGG_w
Cuts at: 346 5066 5666 6187 6865 7139 7286 7697 346
Size: 4720 600 521 678 274 147 411 1126
Fragments arranged by size:
4720 1126 678 600 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 847 877 1473 1503 4007 4037 847
Size: 30 596 30 2504 30 5287
Fragments arranged by size:
5287 2504 596 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 807 852 1374 2398 2428 2453 4845 298
Size: 509 45 522 1024 30 25 2392 3930
Fragments arranged by size:
3930 2392 1024 522 509 45 30 25
BseYI C'CCAG_C
Cuts at: 771 1133 1307 2038 2191 3920 4955 5054 5188
Size: 362 174 731 153 1729 1035 99 134
Cuts at: 5188 5192 5902 6660 7237 7733 771
Size: 4 710 758 577 496 1515
Fragments arranged by size:
1729 1515 1035 758 731 710 577 496
362 174 153 134 99 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 318 1438 2293 3211 168
Size: 150 1120 855 918 5434
Fragments arranged by size:
5434 1120 918 855 150
BsiEI CG_ry'CG
Cuts at: 104 270 4029 5067 6019 6065 7273 104
Size: 166 3759 1038 952 46 1208 1308
Fragments arranged by size:
3759 1308 1208 1038 952 166 46
BsiHKAI G_wGCw'C
Cuts at: 940 2696 2873 3589 4201 4232 4427 5093 5201
Size: 1756 177 716 612 31 195 666 108
Cuts at: 5201 5491 6269 6459 7251 940
Size: 290 778 190 792 2166
Fragments arranged by size:
2166 1756 792 778 716 666 612 290
195 190 177 108 31
BsmI GAATG_Cn'
Cuts at: 1601 1905 3769 1601
Size: 304 1864 6309
Fragments arranged by size:
6309 1864 304
BsmBI CGTCTCn'nnnn_
Cuts at: 613 3535 3835 613
Size: 2922 300 5255
Fragments arranged by size:
5255 2922 300
Bsp1286I G_dGCh'C
Cuts at: 632 892 940 2012 2696 2873 3146 3589 4201
Size: 260 48 1072 684 177 273 443 612
Cuts at: 4201 4232 4312 4356 4385 4427 4502 4902 5093
Size: 31 80 44 29 42 75 400 191
Cuts at: 5093 5165 5201 5491 6102 6195 6269 6459 6521
Size: 72 36 290 611 93 74 190 62
Cuts at: 6521 7251 632
Size: 730 1858
Fragments arranged by size:
1858 1072 730 684 612 611 443 400
290 273 260 191 190 177 93 80
75 74 72 62 48 44 42 36
31 29
BspEI T'CCGG_A
Cuts at: 7697 7697
Size: 8477
BspHI T'CATG_A
Cuts at: 7653 7653
Size: 8477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1404 1455 5430 5527 5741 6043 6424 1404
Size: 51 3975 97 214 302 381 3457
Fragments arranged by size:
3975 3457 381 302 214 97 51
BsrBI CCG'CTC
Cuts at: 50 1993 2443 6769 6823 8307 8472 50
Size: 1943 450 4326 54 1484 165 55
Fragments arranged by size:
4326 1943 1484 450 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1171 6389 1171
Size: 5218 3259
BsrFI r'CCGG_y
Cuts at: 643 2295 2704 4246 4283 6475 6656 6865 643
Size: 1652 409 1542 37 2192 181 209 2255
Fragments arranged by size:
2255 2192 1652 1542 409 209 181 37
BsrGI T'GTAC_A
Cuts at: 4943 4943
Size: 8477
BssHII G'CGCG_C
Cuts at: 4949 4949
Size: 8477
BssSI C'ACGA_G
Cuts at: 1350 4720 4987 5260 6748 7106 1350
Size: 3370 267 273 1488 358 2721
Fragments arranged by size:
3370 2721 1488 358 273 267
BstAPI GCAn_nnn'nTGC
Cuts at: 5854 5854
Size: 8477
BstBI TT'CG_AA
Cuts at: 6838 6838
Size: 8477
BstEII G'GTnAC_C
Cuts at: 901 901
Size: 8477
BstXI CCAn_nnnn'nTGG
Cuts at: 138 2577 3204 3466 138
Size: 2439 627 262 5149
Fragments arranged by size:
5149 2439 627 262
BstYI r'GATC_y
Cuts at: 1 480 1506 2946 3753 4806 4938 5360 5502
Size: 479 1026 1440 807 1053 132 422 142
Cuts at: 5502 5969 5992 6327 6573 7585 7671 7683 8029
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8029 1
Size: 449
Fragments arranged by size:
1440 1053 1026 1012 807 479 467 449
422 346 335 246 142 132 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 920 4546 4764 920
Size: 3626 218 4633
Fragments arranged by size:
4633 3626 218
BtgI C'CryG_G
Cuts at: 354 383 835 1481 1886 2861 4029 4088 4502
Size: 29 452 646 405 975 1168 59 414
Cuts at: 4502 4562 354
Size: 60 4269
Fragments arranged by size:
4269 1168 975 646 452 414 405 60
59 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1146 2149 5092 5196 6427 6608 6771 1146
Size: 1003 2943 104 1231 181 163 2852
Fragments arranged by size:
2943 2852 1231 1003 181 163 104
BtsI GCAGTG_nn'
Cuts at: 3261 4211 4405 5720 3261
Size: 950 194 1315 6018
Fragments arranged by size:
6018 1315 950 194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4450 4485 4863 4898 4993 5028 4450
Size: 35 378 35 95 35 7899
Fragments arranged by size:
7899 378 95 35 35 35
DraI TTT'AAA
Cuts at: 5476 5476
Size: 8477
DraIII CAC_nnn'GTG
Cuts at: 4236 4992 4236
Size: 756 7721
DrdI GACnn_nn'nnGTC
Cuts at: 2821 6183 7041 2821
Size: 3362 858 4257
Fragments arranged by size:
4257 3362 858
EaeI y'GGCC_r
Cuts at: 267 1274 2297 2444 2864 3444 4026 5306 5318
Size: 1007 1023 147 420 580 582 1280 12
Cuts at: 5318 6062 6236 6627 6654 7999 267
Size: 744 174 391 27 1345 745
Fragments arranged by size:
1345 1280 1023 1007 745 744 582 580
420 391 174 147 27 12
EagI C'GGCC_G
Cuts at: 267 4026 6062 267
Size: 3759 2036 2682
Fragments arranged by size:
3759 2682 2036
EarI CTCTTCn'nnn_
Cuts at: 275 572 690 1145 1815 1937 3880 4436 5065
Size: 297 118 455 670 122 1943 556 629
Cuts at: 5065 6500 6710 275
Size: 1435 210 2042
Fragments arranged by size:
2042 1943 1435 670 629 556 455 297
210 122 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5031 6685 6995 7141 8287 5031
Size: 1654 310 146 1146 5221
Fragments arranged by size:
5221 1654 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5489 5489
Size: 8477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 421 848 1169 1574 2168 2644 2938 3066
Size: 189 427 321 405 594 476 294 128
Cuts at: 3066 3488 3779 3904 4354 5077 5179 5371 5470
Size: 422 291 125 450 723 102 192 99
Cuts at: 5470 6302 6734 7481 7749 232
Size: 832 432 747 268 960
Fragments arranged by size:
960 832 747 723 594 476 450 432
427 422 405 321 294 291 268 192
189 128 125 102 99
EcoNI CCTnn'n_nnAGG
Cuts at: 211 871 3150 4767 211
Size: 660 2279 1617 3921
Fragments arranged by size:
3921 2279 1617 660
EcoO109I rG'GnC_Cy
Cuts at: 635 1124 1730 2175 4352 4587 5616 635
Size: 489 606 445 2177 235 1029 3496
Fragments arranged by size:
3496 2177 1029 606 489 445 235
EcoRI G'AATT_C
Cuts at: 4111 5481 4111
Size: 1370 7107
EcoRV GAT'ATC
Cuts at: 2586 2586
Size: 8477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1305 2968 6496 1305
Size: 1663 3528 3286
Fragments arranged by size:
3528 3286 1663
FspI TGC'GCA
Cuts at: 6258 6258
Size: 8477
HaeII r_GCGC'y
Cuts at: 138 2296 2898 3140 3226 4321 5911 6159 7181
Size: 2158 602 242 86 1095 1590 248 1022
Cuts at: 7181 8160 138
Size: 979 455
Fragments arranged by size:
2158 1590 1095 1022 979 602 455 248
242 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 465 490 497 522 4007 4039 4720 4752 5195
Size: 25 7 25 3485 32 681 32 443
Cuts at: 5195 5227 6319 6349 6351 6381 465
Size: 32 1092 30 2 30 2561
Fragments arranged by size:
3485 2561 1092 681 443 32 32 32
30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 3108 4581 4977 5516 3108
Size: 1473 396 539 6069
Fragments arranged by size:
6069 1473 539 396
HindIII A'AGCT_T
Cuts at: 1269 1616 3433 5353 1269
Size: 347 1817 1920 4393
Fragments arranged by size:
4393 1920 1817 347
KasI G'GCGC_C
Cuts at: 134 2292 5907 6155 134
Size: 2158 3615 248 2456
Fragments arranged by size:
3615 2456 2158 248
KpnI G_GTAC'C
Cuts at: 383 5497 383
Size: 5114 3363
MluI A'CGCG_T
Cuts at: 7910 7910
Size: 8477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 2609 3053 3059 4620 5087 7148 7332 713
Size: 1896 444 6 1561 467 2061 184 1858
Fragments arranged by size:
2061 1896 1858 1561 467 444 184 6
MscI TGG'CCA
Cuts at: 3446 5320 6238 3446
Size: 1874 918 5685
Fragments arranged by size:
5685 1874 918
MslI CAynn'nnrTG
Cuts at: 674 829 1090 1462 2404 3410 4204 4567 4724
Size: 155 261 372 942 1006 794 363 157
Cuts at: 4724 5179 5227 5260 6466 6593 674
Size: 455 48 33 1206 127 2558
Fragments arranged by size:
2558 1206 1006 942 794 455 372 363
261 157 155 127 48 33
MspA1I CmG'CkG
Cuts at: 453 1090 2035 3693 4651 5054 5192 5304 5588
Size: 637 945 1658 958 403 138 112 284
Cuts at: 5588 5902 6262 7275 7520 453
Size: 314 360 1013 245 1410
Fragments arranged by size:
1658 1410 1013 958 945 637 403 360
314 284 245 138 112
NaeI GCC'GGC
Cuts at: 2297 4248 4285 6658 2297
Size: 1951 37 2373 4116
Fragments arranged by size:
4116 2373 1951 37
NarI GG'CG_CC
Cuts at: 135 2293 5908 6156 135
Size: 2158 3615 248 2456
Fragments arranged by size:
3615 2456 2158 248
NcoI C'CATG_G
Cuts at: 383 1886 4562 383
Size: 1503 2676 4298
Fragments arranged by size:
4298 2676 1503
NgoMIV G'CCGG_C
Cuts at: 2295 4246 4283 6656 2295
Size: 1951 37 2373 4116
Fragments arranged by size:
4116 2373 1951 37
NspI r_CATG'y
Cuts at: 3190 4800 5530 6030 6937 3190
Size: 1610 730 500 907 4730
Fragments arranged by size:
4730 1610 907 730 500
PciI A'CATG_T
Cuts at: 3186 6933 3186
Size: 3747 4730
PflMI CCAn_nnn'nTGG
Cuts at: 399 399
Size: 8477
PfoI T'CCnGG_A
Cuts at: 523 883 2074 3756 4007 4106 4809 5330 523
Size: 360 1191 1682 251 99 703 521 3670
Fragments arranged by size:
3670 1682 1191 703 521 360 251 99
PmeI GTTT'AAAC
Cuts at: 5476 5476
Size: 8477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7616 7648 7616
Size: 32 8445
PpuMI rG'GwC_Cy
Cuts at: 635 1124 1730 635
Size: 489 606 7382
Fragments arranged by size:
7382 606 489
PshAI GACnn'nnGTC
Cuts at: 3946 3946
Size: 8477
PsiI TTA'TAA
Cuts at: 2780 2780
Size: 8477
PspOMI G'GGCC_C
Cuts at: 4352 4352
Size: 8477
PspXI vC'TCGA_Gb
Cuts at: 3212 3212
Size: 8477
PstI C_TGCA'G
Cuts at: 872 1118 4400 4934 5524 6209 872
Size: 246 3282 534 590 685 3140
Fragments arranged by size:
3282 3140 685 590 534 246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8477
PvuII CAG'CTG
Cuts at: 453 1090 2035 3693 5192 5902 6262 453
Size: 637 945 1658 1499 710 360 2668
Fragments arranged by size:
2668 1658 1499 945 710 637 360
RsrII CG'GwC_CG
Cuts at: 6672 6672
Size: 8477
SacI G_AGCT'C
Cuts at: 5491 5491
Size: 8477
SalI G'TCGA_C
Cuts at: 5514 5514
Size: 8477
SapI GCTCTTCn'nnn_
Cuts at: 690 6500 6710 690
Size: 5810 210 2457
Fragments arranged by size:
5810 2457 210
SbfI CC_TGCA'GG
Cuts at: 4934 5524 4934
Size: 590 7887
ScaI AGT'ACT
Cuts at: 5098 7994 5098
Size: 2896 5581
SfcI C'TryA_G
Cuts at: 33 868 1114 4119 4396 4536 4930 5520 6205
Size: 835 246 3005 277 140 394 590 685
Cuts at: 6205 7198 7389 33
Size: 993 191 1121
Fragments arranged by size:
3005 1121 993 835 685 590 394 277
246 191 140
SfoI GGC'GCC
Cuts at: 136 2294 5909 6157 136
Size: 2158 3615 248 2456
Fragments arranged by size:
3615 2456 2158 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8477
SmaI CCC'GGG
Cuts at: 612 3909 5499 612
Size: 3297 1590 3590
Fragments arranged by size:
3590 3297 1590
SmlI C'TyrA_G
Cuts at: 1077 1920 3212 5116 5356 5632 6547 7039 7301
Size: 843 1292 1904 240 276 915 492 262
Cuts at: 7301 1077
Size: 2253
Fragments arranged by size:
2253 1904 1292 915 843 492 276 262
240
SnaBI TAC'GTA
Cuts at: 3642 3642
Size: 8477
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8477
SphI G_CATG'C
Cuts at: 4800 5530 6030 4800
Size: 730 500 7247
Fragments arranged by size:
7247 730 500
StuI AGG'CCT
Cuts at: 1180 1198 2419 2908 3316 5465 1180
Size: 18 1221 489 408 2149 4192
Fragments arranged by size:
4192 2149 1221 489 408 18
StyI C'CwwG_G
Cuts at: 383 653 1205 1886 4562 5611 5965 383
Size: 270 552 681 2676 1049 354 2895
Fragments arranged by size:
2895 2676 1049 681 552 354 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 631 664 1147 1870 2205 2329 2547 2859
Size: 225 33 483 723 335 124 218 312
Cuts at: 2859 3430 6060 6193 6652 6861 406
Size: 571 2630 133 459 209 2022
Fragments arranged by size:
2630 2022 723 571 483 459 335 312
225 218 209 133 124 33
TatI w'GTAC_w
Cuts at: 4478 4943 5096 7992 4478
Size: 465 153 2896 4963
Fragments arranged by size:
4963 2896 465 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 1473 1505 7994 8026 847
Size: 32 594 32 6489 32 1298
Fragments arranged by size:
6489 1298 594 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 728 6274 728
Size: 5546 2931
XbaI T'CTAG_A
Cuts at: 306 5508 306
Size: 5202 3275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1152 1269 3609 4086 4560 4751 5301 5972 1152
Size: 117 2340 477 474 191 550 671 3657
Fragments arranged by size:
3657 2340 671 550 477 474 191 117
XhoI C'TCGA_G
Cuts at: 3212 3212
Size: 8477
XmaI C'CCGG_G
Cuts at: 610 3907 5497 610
Size: 3297 1590 3590
Fragments arranged by size:
3590 3297 1590
XmnI GAAnn'nnTTC
Cuts at: 2645 2645
Size: 8477
ZraI GAC'GTC
Cuts at: 2855 5271 2855
Size: 2416 6061
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BfrBI BglI
BmtI BsiWI BstZ17I ClaI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI