Restriction map for
pF1KE2487
(Circular) (Six-base) MAPSORT of: pF1KE2487.seq Check: 4996 from: 1 to: 5165
pF1KE2487 5165 bp
With 182 enzymes: SgfI *
October 10, 2013 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1908 1908
Size: 5165
AccI GT'mk_AC
Cuts at: 1622 2203 1622
Size: 581 4584
Acc65I G'GTAC_C
Cuts at: 131 2181 131
Size: 2050 3115
AclI AA'CG_TT
Cuts at: 5005 5005
Size: 5165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 784 2990 3422 4169 4437 390
Size: 394 2206 432 747 268 1118
Fragments arranged by size:
2206 1118 747 432 394 268
AflIII A'CryG_T
Cuts at: 2517 3621 4598 2517
Size: 1104 977 3084
Fragments arranged by size:
3084 1104 977
AgeI A'CCGG_T
Cuts at: 3553 3553
Size: 5165
AhdI GACnn_n'nnGTC
Cuts at: 1044 1044
Size: 5165
AlwNI CAG_nnn'CTG
Cuts at: 2470 4037 2470
Size: 1567 3598
ApaLI G'TGCA_C
Cuts at: 3935 3935
Size: 5165
ApoI r'AATT_y
Cuts at: 547 2169 4518 4774 547
Size: 1622 2349 256 938
Fragments arranged by size:
2349 1622 938 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5165
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
AvaI C'yCGr_G
Cuts at: 959 1463 2185 959
Size: 504 722 3939
Fragments arranged by size:
3939 722 504
BamHI G'GATC_C
Cuts at: 311 2190 311
Size: 1879 3286
BanI G'GyrC_C
Cuts at: 131 540 2181 2595 2843 2878 131
Size: 409 1641 414 248 35 2418
Fragments arranged by size:
2418 1641 414 409 248 35
BanII G_rGCy'C
Cuts at: 355 748 2179 3209 355
Size: 393 1431 1030 2311
Fragments arranged by size:
2311 1431 1030 393
BbeI G_GCGC'C
Cuts at: 2599 2847 2599
Size: 248 4917
BbsI GAAGACnn'nnnn_
Cuts at: 359 482 714 1335 1947 359
Size: 123 232 621 612 3577
Fragments arranged by size:
3577 621 612 232 123
BbvCI CC'TCA_GC
Cuts at: 881 881
Size: 5165
BciVI GTATCCnnnnn_n'
Cuts at: 3063 3824 5165 3063
Size: 761 1341 3063
Fragments arranged by size:
3063 1341 761
BclI T'GATC_A
Cuts at: 612 1521 1646 2685 612
Size: 909 125 1039 3092
Fragments arranged by size:
3092 1039 909 125
BglI GCCn_nnn'nGGC
Cuts at: 1194 1194
Size: 5165
BglII A'GATC_T
Cuts at: 2680 4717 2680
Size: 2037 3128
BlpI GC'TnA_GC
Cuts at: 532 1833 2277 532
Size: 1301 444 3420
Fragments arranged by size:
3420 1301 444
Bme1580I G_kGCm'C
Cuts at: 348 2790 2883 3939 348
Size: 2442 93 1056 1574
Fragments arranged by size:
2442 1574 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1536 2377 2458 2793 4937 1536
Size: 841 81 335 2144 1764
Fragments arranged by size:
2144 1764 841 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2124 3532 2124
Size: 1408 3757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1302 1156
Size: 146 5019
Bpu10I CC'TnA_GC
Cuts at: 747 881 2109 747
Size: 134 1228 3803
Fragments arranged by size:
3803 1228 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1694 1984 2005 2341 3220 3712 4010 661
Size: 1033 290 21 336 879 492 298 1816
Fragments arranged by size:
1816 1033 879 492 336 298 290 21
BsaI GGTCTCn'nnnn_
Cuts at: 1109 4869 1109
Size: 3760 1405
BsaAI yAC'GTr
Cuts at: 2518 2518
Size: 5165
BsaBI GATnn'nnATC
Cuts at: 6 2679 4716 6
Size: 2673 2037 455
Fragments arranged by size:
2673 2037 455
BsaHI Gr'CG_yC
Cuts at: 2596 2844 3546 5042 2596
Size: 248 702 1496 2719
Fragments arranged by size:
2719 1496 702 248
BsaWI w'CCGG_w
Cuts at: 569 2354 2875 3553 3827 3974 4385 569
Size: 1785 521 678 274 147 411 1349
Fragments arranged by size:
1785 1349 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1963 1993 1963
Size: 30 5135
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 109 203 577 634 1246 109
Size: 94 374 57 612 4028
Fragments arranged by size:
4028 612 374 94 57
BseYI C'CCAG_C
Cuts at: 470 829 2590 3348 3925 4421 470
Size: 359 1761 758 577 496 1214
Fragments arranged by size:
1761 1214 758 577 496 359
BsiEI CG_ry'CG
Cuts at: 104 2707 2753 3961 104
Size: 2603 46 1208 1308
Fragments arranged by size:
2603 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2179 2957 3147 3939 2179
Size: 778 190 792 3405
Fragments arranged by size:
3405 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 348 355 748 2179 2790 2883 2957 3147 3209
Size: 7 393 1431 611 93 74 190 62
Cuts at: 3209 3939 348
Size: 730 1574
Fragments arranged by size:
1574 1431 730 611 393 190 93 74
62 7
BspEI T'CCGG_A
Cuts at: 569 4385 569
Size: 3816 1349
BspHI T'CATG_A
Cuts at: 1562 1667 4341 1562
Size: 105 2674 2386
Fragments arranged by size:
2674 2386 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 714 979 1556 1667 1908 2215 2429 2731 3112
Size: 265 577 111 241 307 214 302 381
Cuts at: 3112 714
Size: 2767
Fragments arranged by size:
2767 577 381 307 302 265 241 214
111
BsrBI CCG'CTC
Cuts at: 50 3457 3511 4995 5160 50
Size: 3407 54 1484 165 55
Fragments arranged by size:
3407 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1455 3077 1455
Size: 1622 3543
BsrFI r'CCGG_y
Cuts at: 3163 3344 3553 3163
Size: 181 209 4775
Fragments arranged by size:
4775 209 181
BssSI C'ACGA_G
Cuts at: 1678 3436 3794 1678
Size: 1758 358 3049
Fragments arranged by size:
3049 1758 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2542 2542
Size: 5165
BstBI TT'CG_AA
Cuts at: 3526 3526
Size: 5165
BstEII G'GTnAC_C
Cuts at: 1890 1890
Size: 5165
BstXI CCAn_nnnn'nTGG
Cuts at: 793 793
Size: 5165
BstYI r'GATC_y
Cuts at: 1 311 575 2190 2657 2680 3015 3261 4273
Size: 310 264 1615 467 23 335 246 1012
Cuts at: 4273 4359 4371 4717 1
Size: 86 12 346 449
Fragments arranged by size:
1615 1012 467 449 346 335 310 264
246 86 23 12
BtgI C'CryG_G
Cuts at: 932 1572 932
Size: 640 4525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3115 3296 3459 3115
Size: 181 163 4821
Fragments arranged by size:
4821 181 163
BtsI GCAGTG_nn'
Cuts at: 1368 2408 1368
Size: 1040 4125
DraI TTT'AAA
Cuts at: 2164 2164
Size: 5165
DraIII CAC_nnn'GTG
Cuts at: 1427 1427
Size: 5165
DrdI GACnn_nn'nnGTC
Cuts at: 2871 3729 2871
Size: 858 4307
EaeI y'GGCC_r
Cuts at: 2051 2750 2924 3315 3342 4687 2051
Size: 699 174 391 27 1345 2529
Fragments arranged by size:
2529 1345 699 391 174 27
EagI C'GGCC_G
Cuts at: 2750 2750
Size: 5165
EarI CTCTTCn'nnn_
Cuts at: 418 2070 3188 3398 418
Size: 1652 1118 210 2185
Fragments arranged by size:
2185 1652 1118 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 277 1029 3373 3683 3829 4975 277
Size: 752 2344 310 146 1146 467
Fragments arranged by size:
2344 1146 752 467 310 146
EcoICRI GAG'CTC
Cuts at: 2177 2177
Size: 5165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 784 1156 1302 2990 3422 4169 4437 390
Size: 394 372 146 1688 432 747 268 1118
Fragments arranged by size:
1688 1118 747 432 394 372 268 146
EcoNI CCTnn'n_nnAGG
Cuts at: 655 655
Size: 5165
EcoO109I rG'GnC_Cy
Cuts at: 114 183 208 1140 1545 2118 2304 114
Size: 69 25 932 405 573 186 2975
Fragments arranged by size:
2975 932 573 405 186 69 25
EcoRI G'AATT_C
Cuts at: 2169 2169
Size: 5165
EcoRV GAT'ATC
Cuts at: 697 697
Size: 5165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1950 3184 1950
Size: 1234 3931
FspI TGC'GCA
Cuts at: 2946 2946
Size: 5165
HaeII r_GCGC'y
Cuts at: 836 2599 2847 3869 4848 836
Size: 1763 248 1022 979 1153
Fragments arranged by size:
1763 1153 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 855 887 1030 1062 1672 1692 1704 1724 3007
Size: 32 143 32 610 20 12 20 1283
Cuts at: 3007 3037 3039 3069 855
Size: 30 2 30 2951
Fragments arranged by size:
2951 1283 610 143 32 32 30 30
20 20 12 2
HincII GTy'rAC
Cuts at: 2204 2204
Size: 5165
KasI G'GCGC_C
Cuts at: 2595 2843 2595
Size: 248 4917
KpnI G_GTAC'C
Cuts at: 135 2185 135
Size: 2050 3115
MluI A'CGCG_T
Cuts at: 4598 4598
Size: 5165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 157 3836 4020 157
Size: 3679 184 1302
Fragments arranged by size:
3679 1302 184
MscI TGG'CCA
Cuts at: 2053 2926 2053
Size: 873 4292
MslI CAynn'nnrTG
Cuts at: 3154 3281 3154
Size: 127 5038
MspA1I CmG'CkG
Cuts at: 630 1270 1280 1686 2276 2590 2950 3963 4208
Size: 640 10 406 590 314 360 1013 245
Cuts at: 4208 630
Size: 1587
Fragments arranged by size:
1587 1013 640 590 406 360 314 245
10
NaeI GCC'GGC
Cuts at: 3346 3346
Size: 5165
NarI GG'CG_CC
Cuts at: 2596 2844 2596
Size: 248 4917
NcoI C'CATG_G
Cuts at: 1572 1572
Size: 5165
NgoMIV G'CCGG_C
Cuts at: 3344 3344
Size: 5165
NspI r_CATG'y
Cuts at: 1618 2218 2718 3625 1618
Size: 600 500 907 3158
Fragments arranged by size:
3158 907 600 500
PasI CC'CwG_GG
Cuts at: 739 1410 739
Size: 671 4494
PciI A'CATG_T
Cuts at: 3621 3621
Size: 5165
PflMI CCAn_nnn'nTGG
Cuts at: 249 249
Size: 5165
PfoI T'CCnGG_A
Cuts at: 320 320
Size: 5165
PmeI GTTT'AAAC
Cuts at: 2164 2164
Size: 5165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 5133
PpuMI rG'GwC_Cy
Cuts at: 114 1545 114
Size: 1431 3734
PshAI GACnn'nnGTC
Cuts at: 1637 1771 1637
Size: 134 5031
PstI C_TGCA'G
Cuts at: 1664 1814 1877 1919 2212 2897 1664
Size: 150 63 42 293 685 3932
Fragments arranged by size:
3932 685 293 150 63 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5165
PvuII CAG'CTG
Cuts at: 630 1270 1686 2590 2950 630
Size: 640 416 904 360 2845
Fragments arranged by size:
2845 904 640 416 360
RsrII CG'GwC_CG
Cuts at: 3360 3360
Size: 5165
SacI G_AGCT'C
Cuts at: 2179 2179
Size: 5165
SalI G'TCGA_C
Cuts at: 2202 2202
Size: 5165
SapI GCTCTTCn'nnn_
Cuts at: 3188 3398 3188
Size: 210 4955
SbfI CC_TGCA'GG
Cuts at: 1877 2212 1877
Size: 335 4830
ScaI AGT'ACT
Cuts at: 4682 4682
Size: 5165
SfcI C'TryA_G
Cuts at: 33 1660 1810 1873 1915 2208 2893 3886 4077
Size: 1627 150 63 42 293 685 993 191
Cuts at: 4077 33
Size: 1121
Fragments arranged by size:
1627 1121 993 685 293 191 150 63
42
SfoI GGC'GCC
Cuts at: 2597 2845 2597
Size: 248 4917
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5165
SmaI CCC'GGG
Cuts at: 1465 2187 1465
Size: 722 4443
SmlI C'TyrA_G
Cuts at: 676 1709 1963 2020 2320 3235 3727 3989 676
Size: 1033 254 57 300 915 492 262 1852
Fragments arranged by size:
1852 1033 915 492 300 262 254 57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5165
SphI G_CATG'C
Cuts at: 2218 2718 2218
Size: 500 4665
StuI AGG'CCT
Cuts at: 761 761
Size: 5165
StyI C'CwwG_G
Cuts at: 248 656 727 1572 2299 2653 248
Size: 408 71 845 727 354 2760
Fragments arranged by size:
2760 845 727 408 354 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1034 2748 2881 3340 3549 1034
Size: 1714 133 459 209 2650
Fragments arranged by size:
2650 1714 459 209 133
TatI w'GTAC_w
Cuts at: 4680 4680
Size: 5165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4682 4714 4682
Size: 32 5133
Tth111I GACn'n_nGTC
Cuts at: 1818 2962 1818
Size: 1144 4021
XbaI T'CTAG_A
Cuts at: 2196 2196
Size: 5165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 847 2660 847
Size: 1813 3352
XmaI C'CCGG_G
Cuts at: 1463 2185 1463
Size: 722 4443
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI