Restriction map for
pF1KE2477
(Circular) (Six-base) MAPSORT of: pF1KE2477.seq Check: 5527 from: 1 to: 4925
pF1KE2477 4925 bp
With 182 enzymes: SgfI *
June 18, 2013 11:58 ..
AatII G_ACGT'C
Cuts at: 371 797 371
Size: 426 4499
AccI GT'mk_AC
Cuts at: 1844 1963 1844
Size: 119 4806
Acc65I G'GTAC_C
Cuts at: 1941 1941
Size: 4925
AclI AA'CG_TT
Cuts at: 4765 4765
Size: 4925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1310 2750 3182 3929 4197 265
Size: 1045 1440 432 747 268 993
Fragments arranged by size:
1440 1045 993 747 432 268
AflIII A'CryG_T
Cuts at: 1097 1580 1736 1772 2277 3381 4358 1097
Size: 483 156 36 505 1104 977 1664
Fragments arranged by size:
1664 1104 977 505 483 156 36
AgeI A'CCGG_T
Cuts at: 3313 3313
Size: 4925
AlwNI CAG_nnn'CTG
Cuts at: 1383 1692 2230 3797 1383
Size: 309 538 1567 2511
Fragments arranged by size:
2511 1567 538 309
ApaLI G'TGCA_C
Cuts at: 859 1455 3695 859
Size: 596 2240 2089
Fragments arranged by size:
2240 2089 596
ApoI r'AATT_y
Cuts at: 624 1929 4278 4534 624
Size: 1305 2349 256 1015
Fragments arranged by size:
2349 1305 1015 256
AscI GG'CGCG_CC
Cuts at: 229 229
Size: 4925
AseI AT'TA_AT
Cuts at: 20 20
Size: 4925
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
AvaI C'yCGr_G
Cuts at: 1588 1945 1588
Size: 357 4568
BamHI G'GATC_C
Cuts at: 1619 1950 1619
Size: 331 4594
BanI G'GyrC_C
Cuts at: 112 166 799 1941 2355 2603 2638 112
Size: 54 633 1142 414 248 35 2399
Fragments arranged by size:
2399 1142 633 414 248 54 35
BanII G_rGCy'C
Cuts at: 1229 1939 2969 1229
Size: 710 1030 3185
Fragments arranged by size:
3185 1030 710
BbeI G_GCGC'C
Cuts at: 170 2359 2607 170
Size: 2189 248 2488
Fragments arranged by size:
2488 2189 248
BbsI GAAGACnn'nnnn_
Cuts at: 644 953 644
Size: 309 4616
BciVI GTATCCnnnnn_n'
Cuts at: 250 2823 3584 4925 250
Size: 2573 761 1341 250
Fragments arranged by size:
2573 1341 761 250
BclI T'GATC_A
Cuts at: 2445 2445
Size: 4925
BglII A'GATC_T
Cuts at: 1202 1640 2440 4477 1202
Size: 438 800 2037 1650
Fragments arranged by size:
2037 1650 800 438
BlpI GC'TnA_GC
Cuts at: 2037 2037
Size: 4925
Bme1580I G_kGCm'C
Cuts at: 242 863 1459 2550 2643 3699 242
Size: 621 596 1091 93 1056 1468
Fragments arranged by size:
1468 1091 1056 621 596 93
BmgBI CAC'GTC
Cuts at: 1737 1737
Size: 4925
BmrI ACTGGGnnnn_n'
Cuts at: 1609 2137 2218 2553 4697 1609
Size: 528 81 335 2144 1837
Fragments arranged by size:
2144 1837 528 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1547 3292 1547
Size: 1745 3180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 1835 1324
Size: 511 4414
Bpu10I CC'TnA_GC
Cuts at: 1429 1429
Size: 4925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 2980 3472 3770 2101
Size: 879 492 298 3256
Fragments arranged by size:
3256 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 686 818 4629 686
Size: 132 3811 982
Fragments arranged by size:
3811 982 132
BsaAI yAC'GTr
Cuts at: 1098 1581 2278 1098
Size: 483 697 3745
Fragments arranged by size:
3745 697 483
BsaBI GATnn'nnATC
Cuts at: 6 2439 4476 6
Size: 2433 2037 455
Fragments arranged by size:
2433 2037 455
BsaHI Gr'CG_yC
Cuts at: 167 368 794 2356 2604 3306 4802 167
Size: 201 426 1562 248 702 1496 290
Fragments arranged by size:
1562 1496 702 426 290 248 201
BsaWI w'CCGG_w
Cuts at: 452 2114 2635 3313 3587 3734 4145 452
Size: 1662 521 678 274 147 411 1232
Fragments arranged by size:
1662 1232 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1381 1411 1846 1876 1381
Size: 30 435 30 4430
Fragments arranged by size:
4430 435 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 826 1257 1321 367
Size: 459 431 64 3971
Fragments arranged by size:
3971 459 431 64
BseYI C'CCAG_C
Cuts at: 120 2350 3108 3685 4181 120
Size: 2230 758 577 496 864
Fragments arranged by size:
2230 864 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1136 1136
Size: 4925
BsiEI CG_ry'CG
Cuts at: 104 1919 2467 2513 3721 104
Size: 1815 548 46 1208 1308
Fragments arranged by size:
1815 1308 1208 548 46
BsiHKAI G_wGCw'C
Cuts at: 863 1459 1939 2717 2907 3699 863
Size: 596 480 778 190 792 2089
Fragments arranged by size:
2089 792 778 596 480 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1727 1727
Size: 4925
Bsp1286I G_dGCh'C
Cuts at: 242 863 1229 1459 1939 2550 2643 2717 2907
Size: 621 366 230 480 611 93 74 190
Cuts at: 2907 2969 3699 242
Size: 62 730 1468
Fragments arranged by size:
1468 730 621 611 480 366 230 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4145 4145
Size: 4925
BspHI T'CATG_A
Cuts at: 437 594 4101 437
Size: 157 3507 1261
Fragments arranged by size:
3507 1261 157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1975 2189 2491 2872 1975
Size: 214 302 381 4028
Fragments arranged by size:
4028 381 302 214
BsrBI CCG'CTC
Cuts at: 50 402 3217 3271 4755 4920 50
Size: 352 2815 54 1484 165 55
Fragments arranged by size:
2815 1484 352 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1136 2837 1136
Size: 1701 3224
BsrFI r'CCGG_y
Cuts at: 1612 2923 3104 3313 1612
Size: 1311 181 209 3224
Fragments arranged by size:
3224 1311 209 181
BsrGI T'GTAC_A
Cuts at: 515 740 833 515
Size: 225 93 4607
Fragments arranged by size:
4607 225 93
BssHII G'CGCG_C
Cuts at: 229 229
Size: 4925
BssSI C'ACGA_G
Cuts at: 1405 3196 3554 1405
Size: 1791 358 2776
Fragments arranged by size:
2776 1791 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2302 2302
Size: 4925
BstBI TT'CG_AA
Cuts at: 3286 3286
Size: 4925
BstEII G'GTnAC_C
Cuts at: 1479 1479
Size: 4925
BstYI r'GATC_y
Cuts at: 1 1052 1202 1619 1640 1834 1950 2417 2440
Size: 1051 150 417 21 194 116 467 23
Cuts at: 2440 2775 3021 4033 4119 4131 4477 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1051 1012 467 449 417 346 335 246
194 150 116 86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 1811 1811
Size: 4925
BtgI C'CryG_G
Cuts at: 107 333 527 573 653 107
Size: 226 194 46 80 4379
Fragments arranged by size:
4379 226 194 80 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1341 1593 2875 3056 3219 1341
Size: 252 1282 181 163 3047
Fragments arranged by size:
3047 1282 252 181 163
BtsI GCAGTG_nn'
Cuts at: 778 2168 778
Size: 1390 3535
DraI TTT'AAA
Cuts at: 1924 1924
Size: 4925
DrdI GACnn_nn'nnGTC
Cuts at: 2631 3489 2631
Size: 858 4067
EaeI y'GGCC_r
Cuts at: 148 631 2510 2684 3075 3102 4447 148
Size: 483 1879 174 391 27 1345 626
Fragments arranged by size:
1879 1345 626 483 391 174 27
EagI C'GGCC_G
Cuts at: 2510 2510
Size: 4925
EarI CTCTTCn'nnn_
Cuts at: 472 2948 3158 472
Size: 2476 210 2239
Fragments arranged by size:
2476 2239 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 961 3133 3443 3589 4735 961
Size: 2172 310 146 1146 1151
Fragments arranged by size:
2172 1151 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1937 1937
Size: 4925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1310 1324 1835 2750 3182 3929 4197 265
Size: 1045 14 511 915 432 747 268 993
Fragments arranged by size:
1045 993 915 747 511 432 268 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1306 1306
Size: 4925
EcoO109I rG'GnC_Cy
Cuts at: 569 2064 569
Size: 1495 3430
EcoRI G'AATT_C
Cuts at: 624 1929 624
Size: 1305 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 625 2944 625
Size: 2319 2606
FspI TGC'GCA
Cuts at: 2706 2706
Size: 4925
HaeII r_GCGC'y
Cuts at: 170 1413 2359 2607 3629 4608 170
Size: 1243 946 248 1022 979 487
Fragments arranged by size:
1243 1022 979 946 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 280 569 601 1381 1413 1695 1727 1826
Size: 32 289 32 780 32 282 32 99
Cuts at: 1826 1846 1858 1878 2767 2797 2799 2829 248
Size: 20 12 20 889 30 2 30 2344
Fragments arranged by size:
2344 889 780 289 282 99 32 32
32 32 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1296 1964 1296
Size: 668 4257
HindIII A'AGCT_T
Cuts at: 1084 1084
Size: 4925
KasI G'GCGC_C
Cuts at: 166 2355 2603 166
Size: 2189 248 2488
Fragments arranged by size:
2488 2189 248
KpnI G_GTAC'C
Cuts at: 1945 1945
Size: 4925
MluI A'CGCG_T
Cuts at: 1772 4358 1772
Size: 2586 2339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3596 3780 3596
Size: 184 4741
MscI TGG'CCA
Cuts at: 633 2686 633
Size: 2053 2872
MslI CAynn'nnrTG
Cuts at: 436 721 2914 3041 436
Size: 285 2193 127 2320
Fragments arranged by size:
2320 2193 285 127
MspA1I CmG'CkG
Cuts at: 2036 2350 2710 3723 3968 2036
Size: 314 360 1013 245 2993
Fragments arranged by size:
2993 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1614 3106 1614
Size: 1492 3433
NarI GG'CG_CC
Cuts at: 167 2356 2604 167
Size: 2189 248 2488
Fragments arranged by size:
2488 2189 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4925
NgoMIV G'CCGG_C
Cuts at: 1612 3104 1612
Size: 1492 3433
NspI r_CATG'y
Cuts at: 752 1008 1978 2478 3385 752
Size: 256 970 500 907 2292
Fragments arranged by size:
2292 970 907 500 256
PasI CC'CwG_GG
Cuts at: 1698 1698
Size: 4925
PciI A'CATG_T
Cuts at: 3381 3381
Size: 4925
PmeI GTTT'AAAC
Cuts at: 1924 1924
Size: 4925
PmlI CAC'GTG
Cuts at: 1098 1098
Size: 4925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4893
PshAI GACnn'nnGTC
Cuts at: 602 602
Size: 4925
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 991 1023 991
Size: 32 4893
PstI C_TGCA'G
Cuts at: 323 1972 2657 323
Size: 1649 685 2591
Fragments arranged by size:
2591 1649 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4925
PvuII CAG'CTG
Cuts at: 2350 2710 2350
Size: 360 4565
RsrII CG'GwC_CG
Cuts at: 3120 3120
Size: 4925
SacI G_AGCT'C
Cuts at: 1939 1939
Size: 4925
SalI G'TCGA_C
Cuts at: 1962 1962
Size: 4925
SapI GCTCTTCn'nnn_
Cuts at: 472 2948 3158 472
Size: 2476 210 2239
Fragments arranged by size:
2476 2239 210
SbfI CC_TGCA'GG
Cuts at: 1972 1972
Size: 4925
ScaI AGT'ACT
Cuts at: 1000 4442 1000
Size: 3442 1483
SfcI C'TryA_G
Cuts at: 33 319 1968 2653 3646 3837 33
Size: 286 1649 685 993 191 1121
Fragments arranged by size:
1649 1121 993 685 286 191
SfoI GGC'GCC
Cuts at: 168 2357 2605 168
Size: 2189 248 2488
Fragments arranged by size:
2488 2189 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
SgrAI Cr'CCGG_yG
Cuts at: 1612 1612
Size: 4925
SmaI CCC'GGG
Cuts at: 1590 1947 1590
Size: 357 4568
SmlI C'TyrA_G
Cuts at: 2080 2995 3487 3749 2080
Size: 915 492 262 3256
Fragments arranged by size:
3256 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4925
SphI G_CATG'C
Cuts at: 752 1978 2478 752
Size: 1226 500 3199
Fragments arranged by size:
3199 1226 500
SspI AAT'ATT
Cuts at: 645 645
Size: 4925
StyI C'CwwG_G
Cuts at: 107 201 1023 1110 1892 2059 2413 107
Size: 94 822 87 782 167 354 2619
Fragments arranged by size:
2619 822 782 354 167 94 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 201 2508 2641 3100 3309 100
Size: 101 2307 133 459 209 1716
Fragments arranged by size:
2307 1716 459 209 133 101
TatI w'GTAC_w
Cuts at: 425 515 740 833 998 4440 425
Size: 90 225 93 165 3442 910
Fragments arranged by size:
3442 910 225 165 93 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4442 4474 4442
Size: 32 4893
Tth111I GACn'n_nGTC
Cuts at: 2722 2722
Size: 4925
XbaI T'CTAG_A
Cuts at: 1956 1956
Size: 4925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 217 2420 217
Size: 2203 2722
XmaI C'CCGG_G
Cuts at: 1588 1945 1588
Size: 357 4568
XmnI GAAnn'nnTTC
Cuts at: 466 466
Size: 4925
ZraI GAC'GTC
Cuts at: 369 795 369
Size: 426 4499
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PmlI PpiI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI
BsmI BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PpuMI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SnaBI SrfI StuI SwaI XhoI